BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= 030725E6_G04_e511_14.seq (1524 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC146.05c |cwf25||complexed with Cdc5 protein Cwf25 |Schizosac... 35 0.026 SPAC19G12.07c |rsd1||RNA-binding protein Rsd1|Schizosaccharomyce... 34 0.059 SPAC30D11.09 |cwf19||complexed with Cdc5 protein Cwf19 |Schizosa... 33 0.14 SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase Y|Schizosacch... 31 0.55 SPBC887.05c |cwf29||RNA-binding protein Cwf29|Schizosaccharomyce... 31 0.55 SPBC1604.09c |||exoribonuclease Rex4 |Schizosaccharomyces pombe|... 29 1.7 SPAC6F12.12 |par2|pbp2|protein phosphatase regulatory subunit Pa... 29 2.2 SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 28 3.0 SPBC1711.05 |||nucleocytoplasmic transport chaperone Srp40 |Schi... 27 5.2 SPAC31A2.07c |dbp10||ATP-dependent RNA helicase Dbp10 |Schizosac... 27 5.2 SPBC646.08c |||oxysterol binding protein |Schizosaccharomyces po... 27 6.8 SPAC1D4.14 |tho2|SPAC22F3.14c|THO complex subunit Tho2 |Schizosa... 27 6.8 SPBC146.07 |prp2|mis11|U2AF large subunit |Schizosaccharomyces p... 27 9.0 SPAC4G9.19 |||DNAJ domain protein DNAJB family|Schizosaccharomyc... 27 9.0 >SPBC146.05c |cwf25||complexed with Cdc5 protein Cwf25 |Schizosaccharomyces pombe|chr 2|||Manual Length = 376 Score = 35.1 bits (77), Expect = 0.026 Identities = 19/51 (37%), Positives = 31/51 (60%), Gaps = 3/51 (5%) Frame = +1 Query: 631 RDKHHKSSHHERDRKSEHNKSSSKDSKRHSGDQKSHKRARDESRE---NDR 774 RD+HH+ S+ +R R+ N+ S D + HS ++S++ R+ RE NDR Sbjct: 173 RDRHHR-SNQDRSRERSDNEQHSSDKREHS--RRSYRNDRNNWRERTHNDR 220 Score = 32.3 bits (70), Expect = 0.18 Identities = 14/50 (28%), Positives = 28/50 (56%) Frame = +1 Query: 631 RDKHHKSSHHERDRKSEHNKSSSKDSKRHSGDQKSHKRARDESRENDRSS 780 +++ H+ HH ++ +S D+++HS D++ H R S NDR++ Sbjct: 168 KERRHRDRHHRSNQDRSRERS---DNEQHSSDKREHSR---RSYRNDRNN 211 >SPAC19G12.07c |rsd1||RNA-binding protein Rsd1|Schizosaccharomyces pombe|chr 1|||Manual Length = 604 Score = 33.9 bits (74), Expect = 0.059 Identities = 15/48 (31%), Positives = 25/48 (52%) Frame = +1 Query: 637 KHHKSSHHERDRKSEHNKSSSKDSKRHSGDQKSHKRARDESRENDRSS 780 +H +S +R S +++ D + D +SH+R R SR +RSS Sbjct: 183 RHSRSRRRYEERSSRSSRAHDYDYEDLRDDDRSHERKRSRSRPRERSS 230 Score = 28.7 bits (61), Expect = 2.2 Identities = 14/46 (30%), Positives = 20/46 (43%) Frame = +1 Query: 643 HKSSHHERDRKSEHNKSSSKDSKRHSGDQKSHKRARDESRENDRSS 780 H++ H R S + S+ HS R+ SR+ DRSS Sbjct: 110 HRTPRHHRRSYSPRSDYGSRSPSPHSSVDSHQSRSPVRSRDRDRSS 155 >SPAC30D11.09 |cwf19||complexed with Cdc5 protein Cwf19 |Schizosaccharomyces pombe|chr 1|||Manual Length = 639 Score = 32.7 bits (71), Expect = 0.14 Identities = 15/39 (38%), Positives = 22/39 (56%), Gaps = 2/39 (5%) Frame = +1 Query: 655 HHERDRKSEHNKS--SSKDSKRHSGDQKSHKRARDESRE 765 HH R R+S+H + S + S R +GD+ H+R E E Sbjct: 31 HHSRHRESKHGRHDRSERPSSREAGDRSRHERFSKEPLE 69 >SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase Y|Schizosaccharomyces pombe|chr 1|||Manual Length = 1002 Score = 30.7 bits (66), Expect = 0.55 Identities = 14/43 (32%), Positives = 22/43 (51%), Gaps = 2/43 (4%) Frame = +1 Query: 634 DKHHKSSHHERDRKSEHNKSSSKDSKRHSG--DQKSHKRARDE 756 ++H HH EH+K KD + H G D++ HK +D+ Sbjct: 348 EEHEGPEHHRGPEDKEHHKGP-KDKEHHKGPKDKEHHKGPKDK 389 Score = 29.9 bits (64), Expect = 0.97 Identities = 16/53 (30%), Positives = 25/53 (47%), Gaps = 5/53 (9%) Frame = +1 Query: 631 RDK-HHKS----SHHERDRKSEHNKSSSKDSKRHSGDQKSHKRARDESRENDR 774 +DK HHK HH+ + EH+K KD + H G + + + N+R Sbjct: 378 KDKEHHKGPKDKEHHKGPKDKEHHKGP-KDKEHHKGPKDKEHHQGPKEKHNER 429 Score = 29.1 bits (62), Expect = 1.7 Identities = 15/45 (33%), Positives = 19/45 (42%) Frame = +1 Query: 631 RDKHHKSSHHERDRKSEHNKSSSKDSKRHSGDQKSHKRARDESRE 765 + KHHK H + K H K DS ++ H DE RE Sbjct: 153 KGKHHKGKHAKGKGKKSHPKPED-DSVFFDDERPKHHEFDDEDRE 196 >SPBC887.05c |cwf29||RNA-binding protein Cwf29|Schizosaccharomyces pombe|chr 2|||Manual Length = 217 Score = 30.7 bits (66), Expect = 0.55 Identities = 14/35 (40%), Positives = 20/35 (57%), Gaps = 3/35 (8%) Frame = +1 Query: 655 HHERDRKSEH---NKSSSKDSKRHSGDQKSHKRAR 750 H E+ RK +H ++S DS RHS + H R+R Sbjct: 176 HREKRRKLKHESSDRSDKSDSNRHSRHHRRHSRSR 210 >SPBC1604.09c |||exoribonuclease Rex4 |Schizosaccharomyces pombe|chr 2|||Manual Length = 260 Score = 29.1 bits (62), Expect = 1.7 Identities = 13/43 (30%), Positives = 25/43 (58%) Frame = +1 Query: 649 SSHHERDRKSEHNKSSSKDSKRHSGDQKSHKRARDESRENDRS 777 SS+ E+ +K + S + K+HS ++K K + ++ EN+ S Sbjct: 3 SSNWEKLKKKLDSAKSGRVEKKHSNEKKYLKSVKGKNNENENS 45 >SPAC6F12.12 |par2|pbp2|protein phosphatase regulatory subunit Par2|Schizosaccharomyces pombe|chr 1|||Manual Length = 627 Score = 28.7 bits (61), Expect = 2.2 Identities = 11/43 (25%), Positives = 18/43 (41%) Frame = +1 Query: 634 DKHHKSSHHERDRKSEHNKSSSKDSKRHSGDQKSHKRARDESR 762 D HH S H + KS K + K + H + + K + + Sbjct: 47 DTHHSSKHELKKLKSHFLKDTLKHKRNHHANSNNEKHENSDKK 89 >SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family |Schizosaccharomyces pombe|chr 1|||Manual Length = 1420 Score = 28.3 bits (60), Expect = 3.0 Identities = 15/48 (31%), Positives = 23/48 (47%), Gaps = 1/48 (2%) Frame = +1 Query: 637 KHHKSSHHERDRKSEHNKS-SSKDSKRHSGDQKSHKRARDESRENDRS 777 KH + S HE +S+H SK +++ D+K K SR + S Sbjct: 596 KHKRDSKHEARPESKHESHRESKSAEKDKKDKKDKKEDSKRSRSHSVS 643 >SPBC1711.05 |||nucleocytoplasmic transport chaperone Srp40 |Schizosaccharomyces pombe|chr 2|||Manual Length = 451 Score = 27.5 bits (58), Expect = 5.2 Identities = 17/51 (33%), Positives = 23/51 (45%), Gaps = 2/51 (3%) Frame = +1 Query: 634 DKHHKSSHHERDRKSEHN--KSSSKDSKRHSGDQKSHKRARDESRENDRSS 780 D SS E + SE + SSS DS+ S + S + E+D SS Sbjct: 235 DSSSSSSDSESESSSEDSDSSSSSSDSESESSSKDSDSSSNSSDSEDDSSS 285 >SPAC31A2.07c |dbp10||ATP-dependent RNA helicase Dbp10 |Schizosaccharomyces pombe|chr 1|||Manual Length = 848 Score = 27.5 bits (58), Expect = 5.2 Identities = 15/45 (33%), Positives = 23/45 (51%) Frame = +2 Query: 299 IVKKKMKNPVRTGRIWNEKRPRKIKRSVSTTVKISTVNGREGTPD 433 I+K +K+ VR I N P+K KR++ +K G+PD Sbjct: 307 IIKLPLKDNVRPREIGNVNNPKKRKRALELALK----GSESGSPD 347 >SPBC646.08c |||oxysterol binding protein |Schizosaccharomyces pombe|chr 2|||Manual Length = 516 Score = 27.1 bits (57), Expect = 6.8 Identities = 15/35 (42%), Positives = 20/35 (57%) Frame = +1 Query: 676 SEHNKSSSKDSKRHSGDQKSHKRARDESRENDRSS 780 S KSS+K SK+ S + S + +S NDRSS Sbjct: 188 STMTKSSTKTSKKKSSKKNSKSESNQDS-SNDRSS 221 >SPAC1D4.14 |tho2|SPAC22F3.14c|THO complex subunit Tho2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1628 Score = 27.1 bits (57), Expect = 6.8 Identities = 13/42 (30%), Positives = 22/42 (52%) Frame = +1 Query: 631 RDKHHKSSHHERDRKSEHNKSSSKDSKRHSGDQKSHKRARDE 756 R ++ S++ R+R SE N +S D+KR + K R + Sbjct: 1484 RVSNNNSTNVSRERSSEANHRTSNDNKRDEVTEGKDKNKRQD 1525 >SPBC146.07 |prp2|mis11|U2AF large subunit |Schizosaccharomyces pombe|chr 2|||Manual Length = 517 Score = 26.6 bits (56), Expect = 9.0 Identities = 12/42 (28%), Positives = 20/42 (47%), Gaps = 1/42 (2%) Frame = +1 Query: 637 KHHKSSHHERDRKSEHNKSSSKDSKR-HSGDQKSHKRARDES 759 K H+ E R+ + ++ R H G ++ +R RDES Sbjct: 16 KRHRDYRDEEPRRERGSGGIGREDPRGHYGSERPRRRRRDES 57 >SPAC4G9.19 |||DNAJ domain protein DNAJB family|Schizosaccharomyces pombe|chr 1|||Manual Length = 270 Score = 26.6 bits (56), Expect = 9.0 Identities = 15/53 (28%), Positives = 24/53 (45%), Gaps = 1/53 (1%) Frame = -3 Query: 400 NLHGRRYAPFYLPRPLLVPDPTSPYRILHLLLHYPQ-THPTHLTPRNRNRNRH 245 N H Y L L+ D +H+ Q +HPTH +P+N ++ R+ Sbjct: 80 NKHNEEYFRLLLAANALLSDKRRREEYDRFGIHWNQPSHPTHPSPQNWSQARY 132 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,423,688 Number of Sequences: 5004 Number of extensions: 51041 Number of successful extensions: 246 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 215 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 238 length of database: 2,362,478 effective HSP length: 76 effective length of database: 1,982,174 effective search space used: 854316994 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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