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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 030725E6_G03_e503_13.seq
         (1562 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC22G7.01c ||SPAPJ696.03c|aminopeptidase |Schizosaccharomyces ...   118   2e-27
SPAC1F3.02c |mkh1||MEK kinase |Schizosaccharomyces pombe|chr 1||...    29   1.7  
SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyce...    29   1.7  
SPCC18.01c |adg3|SPCC74.07c|beta-glucosidase Adg3 |Schizosacchar...    28   3.0  

>SPAC22G7.01c ||SPAPJ696.03c|aminopeptidase |Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 598

 Score =  118 bits (285), Expect = 2e-27
 Identities = 95/326 (29%), Positives = 143/326 (43%), Gaps = 1/326 (0%)
 Frame = +3

Query: 99   YNPVFFSYLMITQKSVVLFWGDGKLPLTVQEHLNREGVDGF-EGAGYTEVVERLECLAKE 275
            YNPVFF+Y ++T     L+  + K+   V +HL     DGF +   Y  V    +     
Sbjct: 214  YNPVFFAYSLVTLDEAFLYVDERKVTPEVSKHL-----DGFVKILPYDRVFS--DAKNSN 266

Query: 276  LCQSGDGRHSVWLSSDASEAIHRAASGKNVLKKPLELISEVSPVALAKLIKNEVELEGFR 455
            L + G       +SS  S  I  +     V+  P+     +SP++ AK IKN+ EL+G +
Sbjct: 267  LTRIG-------ISSKTSWCIATSFGETKVM--PI-----LSPISQAKGIKNDAELKGMK 312

Query: 456  NCHVRDGVAVVRFFRWLHDRIDSGDNITEIEAADKLLEFRGDESDFVGPSFETXXXXXXX 635
             CH+RDG A+V +F WL + ++SG+ I E +AA KL +FR   + F+G SFET       
Sbjct: 313  ECHIRDGCALVEYFAWLDEYLNSGNKINEFDAATKLEQFRRKNNLFMGLSFETISSTGPN 372

Query: 636  XXXXHYSPSREGEQTVINPGSMFLLDSGGQYRGWNDRYNKDTGI*VENPTDDQKGAFTAS 815
                HYSP   G   +I+P  ++L DSG QY+       +        P++ ++   T +
Sbjct: 373  GAVIHYSPPATG-SAIIDPTKIYLCDSGAQYKDGTTDVTRTWHF--GEPSEFERQTATLA 429

Query: 816  PQGXR**SAAPYSLRXSXVTCXTVFAXLSLVGTSGLDXAHGXGPRXRPYLNVPEGPSGVF 995
             +G    + A                    +   GLD  HG G     +LNV E P G+ 
Sbjct: 430  LKGHI--ALANIVFPKGTTGYMIDVLARQYLWKYGLDYLHGTGHGVGSFLNVHELPVGIG 487

Query: 996  WXXXXXXXXXXXXXXLSTDPGXSRAG 1073
                            S +PG    G
Sbjct: 488  SREVFNSAPLQAGMVTSNEPGFYEDG 513


>SPAC1F3.02c |mkh1||MEK kinase |Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 1116

 Score = 29.1 bits (62), Expect = 1.7
 Identities = 20/63 (31%), Positives = 33/63 (52%), Gaps = 2/63 (3%)
 Frame = +3

Query: 231  GYTEVVE--RLECLAKELCQSGDGRHSVWLSSDASEAIHRAASGKNVLKKPLELISEVSP 404
            GY+  V+   L C+  E+     GR   W + +A +A+ +  + K     P EL+S+VSP
Sbjct: 1010 GYSAKVDVWSLGCVVLEMLA---GRRP-WSTDEAIQAMFKLGTEKKAPPIPSELVSQVSP 1065

Query: 405  VAL 413
             A+
Sbjct: 1066 EAI 1068


>SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1036

 Score = 29.1 bits (62), Expect = 1.7
 Identities = 32/147 (21%), Positives = 62/147 (42%)
 Frame = -2

Query: 583 SSPRNXXXXXXXXXXXXXSPESILSCSQRKNLTTATPSRT*QFRKPSNSTSFLISLARAT 404
           SS  +             +  S L+ S   + T+A+P+ +      ++S++   SLA ++
Sbjct: 68  SSASSSSLTSSSAASSSLTSSSSLASSSTNSTTSASPTSS----SLTSSSATSSSLASSS 123

Query: 403 GDTSDINSRGFFNTFFPEAALCIASLASLESQTECLPSPL*HSSFAKHSSLSTTSVYPAP 224
             +S + S    ++    +++  +SLAS  + +  L      +S + +S+ S T    A 
Sbjct: 124 TTSSSLASSSITSSSLASSSITSSSLASSSTTSSSL------ASSSTNSTTSATPTSSAT 177

Query: 223 SKPSTPSLLRCSCTVRGSFPSPQNSTT 143
           S   + +    S T      S  NSTT
Sbjct: 178 SSSLSSTAASNSATSSSLASSSLNSTT 204


>SPCC18.01c |adg3|SPCC74.07c|beta-glucosidase Adg3
            |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1131

 Score = 28.3 bits (60), Expect = 3.0
 Identities = 24/80 (30%), Positives = 34/80 (42%), Gaps = 2/80 (2%)
 Frame = -2

Query: 613  VSKDGPTKSDSSPRNXXXXXXXXXXXXXSPESILSCSQRKNLTTATPSRT*QFRKPSNST 434
            +S +  T S SS  +              PES LS S    LT+++ +     R  SNS 
Sbjct: 952  LSTEPKTFSSSSTLSESISSINTNSLTVKPESSLSSSTTSGLTSSSSTIPSSTRSESNSE 1011

Query: 433  SFLISLA--RATGDTSDINS 380
            S   S A  R++  TS + S
Sbjct: 1012 SASTSSASKRSSSSTSLVQS 1031


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 5,211,868
Number of Sequences: 5004
Number of extensions: 106251
Number of successful extensions: 274
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 249
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 273
length of database: 2,362,478
effective HSP length: 76
effective length of database: 1,982,174
effective search space used: 880085256
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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