Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030725E6_G03_e503_13.seq
(1562 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g05350.1 68416.m00583 aminopeptidase P, cytosolic, putative s... 102 6e-22
At4g36760.1 68417.m05216 aminopeptidase P similar to Xaa-Pro ami... 93 5e-19
At4g36760.2 68417.m05215 aminopeptidase P similar to Xaa-Pro ami... 88 2e-17
At3g62800.2 68416.m07056 double-stranded RNA-binding domain (DsR... 33 0.39
At3g62800.1 68416.m07055 double-stranded RNA-binding domain (DsR... 33 0.39
At4g21070.1 68417.m03047 BRCT domain-containing protein / zinc f... 33 0.51
At3g09000.1 68416.m01053 proline-rich family protein 31 2.1
At1g30050.1 68414.m03674 hypothetical protein 31 2.1
At3g57380.1 68416.m06387 expressed protein contains Pfam domain,... 29 6.3
At5g34910.1 68418.m04116 hypothetical protein 29 8.3
>At3g05350.1 68416.m00583 aminopeptidase P, cytosolic, putative
similar to cytosolic aminopeptidase P from [Homo sapiens]
GI:8489879, [Rattus norvegicus] GI:2760920; contains Pfam
profile PF00557: metallopeptidase family M24
Length = 569
Score = 102 bits (245), Expect = 6e-22
Identities = 92/336 (27%), Positives = 138/336 (41%), Gaps = 38/336 (11%)
Frame = +3
Query: 99 YNPVFFSYLMITQKSVVLFWGDGKLPLTVQEHLNREGVDGFEGAGYTEVVERLECLAKEL 278
++PV ++YL++ LF + K+ + V++HL G+ E Y +++ ++ LA
Sbjct: 128 HSPVMYAYLIVEVDQAQLFVDNSKVTVEVKDHLKNAGI---ELRPYDSILQGIDSLAARG 184
Query: 279 CQ-----------------SGDGRHSVWLSSDASEAIHRAASGKNVLKKPLELISEVSPV 407
Q S R+S S+A S P + + SP+
Sbjct: 185 AQLLMDPSTLNVAIISTYKSACERYSRNFESEAKVKTKFTDSSSGYTANPSGIYMQ-SPI 243
Query: 408 ALAKLIKNEVELEGFRNCHVRDGVAVVRFFRWLHDRIDSGDNITEIEAADKLLEFRGDES 587
+ AK IKN+ EL+G +N H+RD A+ F+ WL + + N+TE++ AD+LLEFR +
Sbjct: 244 SWAKAIKNDAELKGMKNSHLRDAAALAHFWAWLEEEVHKNANLTEVDVADRLLEFRSMQD 303
Query: 588 DFVGPSFET---------------------XXXXXXXXXXXHYSPSREGEQTVINPGSMF 704
F+ SF+T HY P E V +P +F
Sbjct: 304 GFMDTSFDTISGMVIVTLVGGTCFLTHSQYTASSGANGAIIHYKPEPESCSRV-DPQKLF 362
Query: 705 LLDSGGQYRGWNDRYNKDTGI*VENPTDDQKGAFTASPQGXR**SAAPYSLRXSXVTCXT 884
LLDSG QY + + P+ +K FT QG A +
Sbjct: 363 LLDSGAQYVDGTTDITRT--VHFSEPSAREKECFTRVLQGHIALDQAVFPEGTPGFVLDG 420
Query: 885 VFAXLSLVGTSGLDXAHGXGPRXRPYLNVPEGPSGV 992
FA SL GLD HG G LNV EGP +
Sbjct: 421 -FARSSL-WKIGLDYRHGTGHGVGAALNVHEGPQSI 454
>At4g36760.1 68417.m05216 aminopeptidase P similar to Xaa-Pro
aminopeptidase 2 [Lycopersicon esculentum] GI:15384991;
contains Pfam profile PF00557: metallopeptidase family
M24
Length = 634
Score = 93.1 bits (221), Expect = 5e-19
Identities = 96/357 (26%), Positives = 147/357 (41%), Gaps = 32/357 (8%)
Frame = +3
Query: 99 YNPVFFSYLMITQKSVVLFWGDGKLPLTVQEHLNREGVDGFEGAGYTEVVERLECLAKEL 278
Y PV ++ ++T S L+ K+ + N GV+ E YT+V+ + LA +
Sbjct: 197 YCPVVHAFAILTTDSAFLYVDKKKVSDEANSYFNGLGVEVRE---YTDVISDVALLASDR 253
Query: 279 CQSGDGRHSVW--------LSSDASEAIH-RAASGKNVLKKPLE---LISEVSPVALAKL 422
S +V + SD + + AS L L+ ++ + SP++L+K
Sbjct: 254 LISSFASKTVQHEAAKDMEIDSDQPDRLWVDPASCCYALYSKLDAEKVLLQPSPISLSKA 313
Query: 423 IKNEVELEGFRNCHVRDGVAVVRFFRWLHDRID-----SG---------------DNITE 542
+KN VELEG +N HVRDG AVV++ WL +++ SG +TE
Sbjct: 314 LKNPVELEGIKNAHVRDGAAVVQYLVWLDNQMQELYGASGYFLEAEASKKKPSETSKLTE 373
Query: 543 IEAADKLLEFRGDESDFVGPSFETXXXXXXXXXXXHYSPSREGEQTVINPGSMFLLDSGG 722
+ +DKL R + F G SF T HYSP E ++P ++L DSG
Sbjct: 374 VTVSDKLESLRASKEHFRGLSFPTISSVGSNAAVIHYSPEPEA-CAEMDPDKIYLCDSGA 432
Query: 723 QYRGWNDRYNKDTGI*VENPTDDQKGAFTASPQGXR**SAAPYSLRXSXVTCXTVFAXLS 902
QY + + P+ +K +TA +G A + + T + A
Sbjct: 433 QYLDGTTDITRT--VHFGKPSAHEKECYTAVFKGHVALGNARFPKGTNGYT-LDILARAP 489
Query: 903 LVGTSGLDXAHGXGPRXRPYLNVPEGPSGVFWXXXXXXXXXXXXXXLSTDPGXSRAG 1073
L GLD HG G YL V EGP V + ++ +PG G
Sbjct: 490 L-WKYGLDYRHGTGHGVGSYLCVHEGPHQVSFRPSARNVPLQATMTVTDEPGYYEDG 545
>At4g36760.2 68417.m05215 aminopeptidase P similar to Xaa-Pro
aminopeptidase 2 [Lycopersicon esculentum] GI:15384991;
contains Pfam profile PF00557: metallopeptidase family
M24
Length = 519
Score = 87.8 bits (208), Expect = 2e-17
Identities = 91/326 (27%), Positives = 138/326 (42%), Gaps = 32/326 (9%)
Frame = +3
Query: 99 YNPVFFSYLMITQKSVVLFWGDGKLPLTVQEHLNREGVDGFEGAGYTEVVERLECLAKEL 278
Y PV ++ ++T S L+ K+ + N GV+ E YT+V+ + LA +
Sbjct: 197 YCPVVHAFAILTTDSAFLYVDKKKVSDEANSYFNGLGVEVRE---YTDVISDVALLASDR 253
Query: 279 CQSGDGRHSVW--------LSSDASEAIH-RAASGKNVLKKPLE---LISEVSPVALAKL 422
S +V + SD + + AS L L+ ++ + SP++L+K
Sbjct: 254 LISSFASKTVQHEAAKDMEIDSDQPDRLWVDPASCCYALYSKLDAEKVLLQPSPISLSKA 313
Query: 423 IKNEVELEGFRNCHVRDGVAVVRFFRWLHDRID-----SG---------------DNITE 542
+KN VELEG +N HVRDG AVV++ WL +++ SG +TE
Sbjct: 314 LKNPVELEGIKNAHVRDGAAVVQYLVWLDNQMQELYGASGYFLEAEASKKKPSETSKLTE 373
Query: 543 IEAADKLLEFRGDESDFVGPSFETXXXXXXXXXXXHYSPSREGEQTVINPGSMFLLDSGG 722
+ +DKL R + F G SF T HYSP E ++P ++L DSG
Sbjct: 374 VTVSDKLESLRASKEHFRGLSFPTISSVGSNAAVIHYSPEPEA-CAEMDPDKIYLCDSGA 432
Query: 723 QYRGWNDRYNKDTGI*VENPTDDQKGAFTASPQGXR**SAAPYSLRXSXVTCXTVFAXLS 902
QY + + P+ +K +TA +G A + + T + A
Sbjct: 433 QYLDGTTDITRT--VHFGKPSAHEKECYTAVFKGHVALGNARFPKGTNGYT-LDILARAP 489
Query: 903 LVGTSGLDXAHGXGPRXRPYLNVPEG 980
L GLD HG G YL V EG
Sbjct: 490 L-WKYGLDYRHGTGHGVGSYLCVHEG 514
>At3g62800.2 68416.m07056 double-stranded RNA-binding domain
(DsRBD)-containing protein weak similarity to SP|P19525
Interferon-induced, double-stranded RNA-activated
protein kinase (EC 2.7.1.-) {Homo sapiens}; contains
Pfam profile PF00035: Double-stranded RNA binding motif
Length = 355
Score = 33.5 bits (73), Expect = 0.39
Identities = 17/57 (29%), Positives = 29/57 (50%)
Frame = +1
Query: 631 PTGLLSTTAPQGRANRR**ILAPCSSWIPVDSTGDGTTDITRTPAYESKTRLMIRRG 801
PT +++ AP +R +A CSSW+P + T DG+ + K +L++ G
Sbjct: 259 PTNVITLNAPAANGIKR--NIAACSSWMPQNPTNDGSETSSCVVDESEKKKLIMGTG 313
>At3g62800.1 68416.m07055 double-stranded RNA-binding domain
(DsRBD)-containing protein weak similarity to SP|P19525
Interferon-induced, double-stranded RNA-activated
protein kinase (EC 2.7.1.-) {Homo sapiens}; contains
Pfam profile PF00035: Double-stranded RNA binding motif
Length = 355
Score = 33.5 bits (73), Expect = 0.39
Identities = 17/57 (29%), Positives = 29/57 (50%)
Frame = +1
Query: 631 PTGLLSTTAPQGRANRR**ILAPCSSWIPVDSTGDGTTDITRTPAYESKTRLMIRRG 801
PT +++ AP +R +A CSSW+P + T DG+ + K +L++ G
Sbjct: 259 PTNVITLNAPAANGIKR--NIAACSSWMPQNPTNDGSETSSCVVDESEKKKLIMGTG 313
>At4g21070.1 68417.m03047 BRCT domain-containing protein / zinc
finger (C3HC4-type RING finger) family protein (BRCA1)
contains Pfam profiles PF00533: BRCA1 C Terminus (BRCT)
domain, PF00097: Zinc finger, C3HC4 type (RING finger),
PF01535: PPR repeat; identical to cDNA BRCA1 GI:28372473
Length = 1276
Score = 33.1 bits (72), Expect = 0.51
Identities = 27/70 (38%), Positives = 33/70 (47%)
Frame = +3
Query: 495 FRWLHDRIDSGDNITEIEAADKLLEFRGDESDFVGPSFETXXXXXXXXXXXHYSPSREGE 674
F WL D D G+N ++ +D+LL G V PSF H SPS+E E
Sbjct: 540 FFWLRDE-DDGENSSQRTESDQLL---GTTPVNV-PSFSDLMDSD------HESPSKEDE 588
Query: 675 QTVINPGSMF 704
Q NPG MF
Sbjct: 589 QQKPNPGDMF 598
>At3g09000.1 68416.m01053 proline-rich family protein
Length = 541
Score = 31.1 bits (67), Expect = 2.1
Identities = 32/123 (26%), Positives = 47/123 (38%), Gaps = 5/123 (4%)
Frame = -2
Query: 511 SCSQRKNLTTATPSRT*QFRKPSNSTSFLISLARATGDTSDINSRGFFN-----TFFPEA 347
S S R ATP+R + P+ STS ++ + +S SR T
Sbjct: 150 SGSSRSTSRPATPTR--RSTTPTTSTSRPVTTRASNSRSSTPTSRATLTAARATTSTAAP 207
Query: 346 ALCIASLASLESQTECLPSPL*HSSFAKHSSLSTTSVYPAPSKPSTPSLLRCSCTVRGSF 167
S S S T +P S+ +K + PS P+ PS++ RG+
Sbjct: 208 RTTTTSSGSARSATPTRSNPRPSSASSKKPVSRPATPTRRPSTPTGPSIVSSKAPSRGTS 267
Query: 166 PSP 158
PSP
Sbjct: 268 PSP 270
>At1g30050.1 68414.m03674 hypothetical protein
Length = 389
Score = 31.1 bits (67), Expect = 2.1
Identities = 20/60 (33%), Positives = 29/60 (48%)
Frame = -2
Query: 319 LESQTECLPSPL*HSSFAKHSSLSTTSVYPAPSKPSTPSLLRCSCTVRGSFPSPQNSTTD 140
+E E PS S A +S+ S+ P+PS P++PS L S + Q+ST D
Sbjct: 297 IEEVAEVNPSITRTLSMASYSASELPSISPSPSSPASPSRLSVSTDIYPILVQ-QSSTND 355
>At3g57380.1 68416.m06387 expressed protein contains Pfam domain,
PF04577: Protein of unknown function (DUF563)
Length = 504
Score = 29.5 bits (63), Expect = 6.3
Identities = 21/70 (30%), Positives = 33/70 (47%), Gaps = 4/70 (5%)
Frame = -1
Query: 701 HGARIYHRLFALP*GAVVDNSPVGT----SSRYGLERRSHEIRFIPAKLQEFISSFYLSD 534
HGA + H LF P + P+GT + YG + +++I K++ SS Y D
Sbjct: 379 HGAAMTHLLFLKPKTVFIQIIPIGTEWAAETYYGKPAKKMRLKYIGYKIKPKESSLY--D 436
Query: 533 IITGIDPIVQ 504
DPI++
Sbjct: 437 EYGIDDPIIR 446
>At5g34910.1 68418.m04116 hypothetical protein
Length = 203
Score = 29.1 bits (62), Expect = 8.3
Identities = 13/45 (28%), Positives = 22/45 (48%), Gaps = 1/45 (2%)
Frame = +1
Query: 925 TXPTXPDPXVGPT*TSPRVRPA-SSGSPXLXIPGLXPXXXXAPTL 1056
T T P+P PT T+P P ++ +P + + G P + T+
Sbjct: 152 TDSTEPEPETHPTTTTPSTLPTPTTTAPAIIVAGTTPLVSTSATI 196
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 29,594,555
Number of Sequences: 28952
Number of extensions: 659967
Number of successful extensions: 1860
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1570
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1829
length of database: 12,070,560
effective HSP length: 84
effective length of database: 9,638,592
effective search space used: 4202426112
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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