Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030725E6_G02_e495_14.seq
(1532 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC685.06 |rps001|rps0-1, rpsa-1, rps0|40S ribosomal protein S0... 200 4e-52
SPAPJ698.02c |rps002|rpsa-2, rps0-2, rps0|40S ribosomal protein ... 198 2e-51
SPAC24C9.10c |mrp4||mitochondrial ribosomal protein subunit S2|S... 42 3e-04
SPAC17C9.13c |cut8||tethering factor for nuclear proteasome Cut8... 29 2.3
SPAC27E2.06c |||methionine-tRNA ligase, mitochondrial|Schizosacc... 29 2.3
SPCC1840.08c |||protein disulfide isomerase |Schizosaccharomyces... 27 6.9
SPAC24B11.10c |chr3|cfh1|chitin synthase regulatory factor Chr3 ... 27 6.9
>SPBC685.06 |rps001|rps0-1, rpsa-1, rps0|40S ribosomal protein S0A
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 292
Score = 200 bits (488), Expect = 4e-52
Identities = 95/137 (69%), Positives = 109/137 (79%)
Frame = +1
Query: 103 DPADVFVISSRAFGQRAVLKFAAHTGATPIAGRFTPGAFTNQIQAAFREPRLLIVLDPAQ 282
+PADV V+S+R +G RAVLKFAAHTGAT IAGRFTPG FTN I +REPRL++V DP
Sbjct: 72 NPADVCVVSTRTYGHRAVLKFAAHTGATAIAGRFTPGNFTNYITRTYREPRLIVVTDPRA 131
Query: 283 DHQPITEASYVNIPVIALCNTDSPLRFVDIAIPCNTKSSHSIGLMWWLLAREVLRLRGVL 462
D Q I EAS+VNIPVIALC+TDS L VDIAIP N K SIGL+W+LLAREVLR+RG L
Sbjct: 132 DAQAIKEASFVNIPVIALCDTDSILNHVDIAIPTNNKGRKSIGLIWYLLAREVLRVRGTL 191
Query: 463 SRDQRWDVVVDLFFYRD 513
SR WDV+ DL+FYRD
Sbjct: 192 SRSAPWDVMPDLYFYRD 208
>SPAPJ698.02c |rps002|rpsa-2, rps0-2, rps0|40S ribosomal protein
S0B|Schizosaccharomyces pombe|chr 1|||Manual
Length = 287
Score = 198 bits (482), Expect = 2e-51
Identities = 96/137 (70%), Positives = 108/137 (78%)
Frame = +1
Query: 103 DPADVFVISSRAFGQRAVLKFAAHTGATPIAGRFTPGAFTNQIQAAFREPRLLIVLDPAQ 282
+PADV VISSR +G RAVLKFAAHTGAT IAGRFTPG FTN I +REPRL+IV DP
Sbjct: 73 NPADVCVISSRPYGHRAVLKFAAHTGATAIAGRFTPGNFTNYITRTYREPRLIIVTDPRA 132
Query: 283 DHQPITEASYVNIPVIALCNTDSPLRFVDIAIPCNTKSSHSIGLMWWLLAREVLRLRGVL 462
D Q I EAS+VNIPVIALC+TDS L VD+AIP N K SIGL W+LLAREVLRLRG +
Sbjct: 133 DAQAIKEASFVNIPVIALCDTDSILNHVDVAIPINNKGYKSIGLAWYLLAREVLRLRGNI 192
Query: 463 SRDQRWDVVVDLFFYRD 513
SR W+V+ DL+FYRD
Sbjct: 193 SRTTAWEVMPDLYFYRD 209
>SPAC24C9.10c |mrp4||mitochondrial ribosomal protein subunit
S2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 263
Score = 41.5 bits (93), Expect = 3e-04
Identities = 20/62 (32%), Positives = 33/62 (53%)
Frame = +1
Query: 250 PRLLIVLDPAQDHQPITEASYVNIPVIALCNTDSPLRFVDIAIPCNTKSSHSIGLMWWLL 429
P L+++L+P ++ EA ++P I + +TD+ R V IP N S L+ LL
Sbjct: 180 PDLMVILNPLENKSACLEAQKTHVPTIGIIDTDADPRMVTYPIPANDDSLRCTDLIAGLL 239
Query: 430 AR 435
+R
Sbjct: 240 SR 241
>SPAC17C9.13c |cut8||tethering factor for nuclear proteasome
Cut8|Schizosaccharomyces pombe|chr 1|||Manual
Length = 262
Score = 28.7 bits (61), Expect = 2.3
Identities = 16/61 (26%), Positives = 33/61 (54%)
Frame = -2
Query: 655 PVLHLQPMMQLVQHSQSIPHELAAALPLWERRQPVP*LVALPSHSLQGHDRRTSQRQHPN 476
PV+ L P+ +L +H+++ + + AL ++ Q LV SH+ Q + + + +H N
Sbjct: 184 PVVPLLPLEELEEHNKTSQNRMEEALNYLKQLQKNEPLVHERSHTFQQTNPQNNFHRHTN 243
Query: 475 A 473
+
Sbjct: 244 S 244
>SPAC27E2.06c |||methionine-tRNA ligase,
mitochondrial|Schizosaccharomyces pombe|chr 1|||Manual
Length = 539
Score = 28.7 bits (61), Expect = 2.3
Identities = 23/84 (27%), Positives = 38/84 (45%), Gaps = 5/84 (5%)
Frame = +1
Query: 172 HTGATPIAGRFTPGAFTNQIQAAFR--EPRLLIVLDPAQDHQPI---TEASYVNIPVIAL 336
+ A P G T+ I A F+ +P + ++ D + T A + + L
Sbjct: 29 YVNAAPHLGHLYSLVLTDAI-ARFQNLKPDVSVISSTGTDEHGLKVQTVAQTEGVSPLQL 87
Query: 337 CNTDSPLRFVDIAIPCNTKSSHSI 408
C+ +S RF D+A+ NTK +H I
Sbjct: 88 CDRNSK-RFADLAVAANTKFTHFI 110
>SPCC1840.08c |||protein disulfide isomerase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 561
Score = 27.1 bits (57), Expect = 6.9
Identities = 12/26 (46%), Positives = 17/26 (65%)
Frame = +1
Query: 406 IGLMWWLLAREVLRLRGVLSRDQRWD 483
IGL W L REV R + + +R++ WD
Sbjct: 365 IGLKWTLKLREVERKQLLTAREKWWD 390
>SPAC24B11.10c |chr3|cfh1|chitin synthase regulatory factor Chr3
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 932
Score = 27.1 bits (57), Expect = 6.9
Identities = 17/47 (36%), Positives = 22/47 (46%)
Frame = +2
Query: 941 TWENPGVTNLIALQHIPLSPAGVIAXRPARSPXPTVAXPEWRMANXS 1081
T +NP +L+ LQ P P + PARSP V P + N S
Sbjct: 341 TLKNPRNDDLLTLQQPPRYPHSQMVMLPARSPV-EVPAPTFDRRNVS 386
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 4,515,912
Number of Sequences: 5004
Number of extensions: 78969
Number of successful extensions: 189
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 187
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 189
length of database: 2,362,478
effective HSP length: 76
effective length of database: 1,982,174
effective search space used: 860263516
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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