Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030725E6_G01_e487_13.seq
(1526 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 412 e-116
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 412 e-116
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 412 e-116
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 136 6e-33
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 129 9e-31
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 93 1e-19
SPBC21D10.06c |map4||cell agglutination protein Map4|Schizosacch... 31 0.42
SPAC631.01c |acp2||F-actin capping protein beta subunit |Schizos... 27 9.0
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 412 bits (1015), Expect = e-116
Identities = 187/250 (74%), Positives = 218/250 (87%)
Frame = +2
Query: 80 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSESRFEEIKKEVSSYIKK 259
GEFEAGISK+GQTREHALLA+TLGVKQLIV VNKMD+T +S++RFEEI KE S++IKK
Sbjct: 121 GEFEAGISKDGQTREHALLAYTLGVKQLIVAVNKMDTTG--WSQARFEEIVKETSNFIKK 178
Query: 260 IGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKGWLVERKEGKAEGKCLIEALDAILPPAR 439
+G+NP V FVP+SG+ GDNM+EP+T MPW++GW E K G +GK L+EA+D+I PPAR
Sbjct: 179 VGFNPKTVPFVPVSGFQGDNMIEPTTNMPWYQGWQKETKAGVVKGKTLLEAIDSIEPPAR 238
Query: 440 PTDKALRLPLQDVYKIGGIGTVPVGRVETGILKPGTVVVFAPANITTEVKSVEMHHEALQ 619
PTDK LRLPLQDVYKIGGIGTVPVGRVETG++KPG +V FAPA +TTEVKSVEMHHE+L
Sbjct: 239 PTDKPLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMIVTFAPAGVTTEVKSVEMHHESLD 298
Query: 620 EAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPRGAADFTAQVIVLNHPGQISNGYTPV 799
+PGDNVGFNVKNVSVK++RRG V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PV
Sbjct: 299 AGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPV 358
Query: 800 LDCHTAHIAC 829
LDCHTAHIAC
Sbjct: 359 LDCHTAHIAC 368
Score = 55.2 bits (127), Expect = 2e-08
Identities = 25/57 (43%), Positives = 38/57 (66%), Gaps = 1/57 (1%)
Frame = +1
Query: 811 HSPH-SLQFAEIKEKVDRRTGKSTEDXLKSIKSGDAXIVNLVXFQPLCVXSXQXFHP 978
H+ H + +FAE+ EK+DRR+GK E+ K +KSGDA I +V +P+CV + + P
Sbjct: 362 HTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAP 418
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 412 bits (1015), Expect = e-116
Identities = 187/250 (74%), Positives = 218/250 (87%)
Frame = +2
Query: 80 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSESRFEEIKKEVSSYIKK 259
GEFEAGISK+GQTREHALLA+TLGVKQLIV VNKMD+T +S++RFEEI KE S++IKK
Sbjct: 121 GEFEAGISKDGQTREHALLAYTLGVKQLIVAVNKMDTTG--WSQARFEEIVKETSNFIKK 178
Query: 260 IGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKGWLVERKEGKAEGKCLIEALDAILPPAR 439
+G+NP V FVP+SG+ GDNM+EP+T MPW++GW E K G +GK L+EA+D+I PPAR
Sbjct: 179 VGFNPKTVPFVPVSGFQGDNMIEPTTNMPWYQGWQKETKAGVVKGKTLLEAIDSIEPPAR 238
Query: 440 PTDKALRLPLQDVYKIGGIGTVPVGRVETGILKPGTVVVFAPANITTEVKSVEMHHEALQ 619
PTDK LRLPLQDVYKIGGIGTVPVGRVETG++KPG +V FAPA +TTEVKSVEMHHE+L
Sbjct: 239 PTDKPLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMIVTFAPAGVTTEVKSVEMHHESLD 298
Query: 620 EAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPRGAADFTAQVIVLNHPGQISNGYTPV 799
+PGDNVGFNVKNVSVK++RRG V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PV
Sbjct: 299 AGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPV 358
Query: 800 LDCHTAHIAC 829
LDCHTAHIAC
Sbjct: 359 LDCHTAHIAC 368
Score = 55.2 bits (127), Expect = 2e-08
Identities = 25/57 (43%), Positives = 38/57 (66%), Gaps = 1/57 (1%)
Frame = +1
Query: 811 HSPH-SLQFAEIKEKVDRRTGKSTEDXLKSIKSGDAXIVNLVXFQPLCVXSXQXFHP 978
H+ H + +FAE+ EK+DRR+GK E+ K +KSGDA I +V +P+CV + + P
Sbjct: 362 HTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAP 418
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 412 bits (1015), Expect = e-116
Identities = 187/250 (74%), Positives = 218/250 (87%)
Frame = +2
Query: 80 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSESRFEEIKKEVSSYIKK 259
GEFEAGISK+GQTREHALLA+TLGVKQLIV VNKMD+T +S++RFEEI KE S++IKK
Sbjct: 121 GEFEAGISKDGQTREHALLAYTLGVKQLIVAVNKMDTTG--WSQARFEEIVKETSNFIKK 178
Query: 260 IGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKGWLVERKEGKAEGKCLIEALDAILPPAR 439
+G+NP V FVP+SG+ GDNM+EP+T MPW++GW E K G +GK L+EA+D+I PPAR
Sbjct: 179 VGFNPKTVPFVPVSGFQGDNMIEPTTNMPWYQGWQKETKAGVVKGKTLLEAIDSIEPPAR 238
Query: 440 PTDKALRLPLQDVYKIGGIGTVPVGRVETGILKPGTVVVFAPANITTEVKSVEMHHEALQ 619
PTDK LRLPLQDVYKIGGIGTVPVGRVETG++KPG +V FAPA +TTEVKSVEMHHE+L
Sbjct: 239 PTDKPLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMIVTFAPAGVTTEVKSVEMHHESLD 298
Query: 620 EAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPRGAADFTAQVIVLNHPGQISNGYTPV 799
+PGDNVGFNVKNVSVK++RRG V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PV
Sbjct: 299 AGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPV 358
Query: 800 LDCHTAHIAC 829
LDCHTAHIAC
Sbjct: 359 LDCHTAHIAC 368
Score = 55.2 bits (127), Expect = 2e-08
Identities = 25/57 (43%), Positives = 38/57 (66%), Gaps = 1/57 (1%)
Frame = +1
Query: 811 HSPH-SLQFAEIKEKVDRRTGKSTEDXLKSIKSGDAXIVNLVXFQPLCVXSXQXFHP 978
H+ H + +FAE+ EK+DRR+GK E+ K +KSGDA I +V +P+CV + + P
Sbjct: 362 HTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAP 418
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 136 bits (330), Expect = 6e-33
Identities = 82/251 (32%), Positives = 136/251 (54%), Gaps = 5/251 (1%)
Frame = +2
Query: 80 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSESRFEEIKKEVSSYIKK 259
GEFEAG + GQTREHA+LA T G+ L+V +NKMD +SE R++E ++S ++++
Sbjct: 352 GEFEAGFERGGQTREHAVLARTQGINHLVVVINKMDEPSVQWSEERYKECVDKLSMFLRR 411
Query: 260 I-GYNPAA-VAFVPISGWHGDNMLE--PSTKMPWFKGWLVERKEGKAEGKCLIEALDAIL 427
+ GYN V ++P+S + G N+ + S+ PW+ +G L+E LD++
Sbjct: 412 VAGYNSKTDVKYMPVSAYTGQNVKDRVDSSVCPWY------------QGPSLLEYLDSMT 459
Query: 428 PPARPTDKALRLPLQDVYKIGGIGTVPVGRVETGILKPGTVVVFAPANITTEVKSV-EMH 604
R + +P+ YK +GT+ G++E G +K + V+ P N T EV ++ +
Sbjct: 460 HLERKVNAPFIMPIASKYK--DLGTILEGKIEAGSIKKNSNVLVMPINQTLEVTAIYDEA 517
Query: 605 HEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPRGAADFTAQVIVLNHPGQISN 784
E + ++ GD V V+ +++ GYV +K NP F AQ+ +L P ++
Sbjct: 518 DEEISSSICGDQVRLRVRG-DDSDVQTGYVLTSTK-NPVHATTRFIAQIAILELPSILTT 575
Query: 785 GYTPVLDCHTA 817
GY+ V+ HTA
Sbjct: 576 GYSCVMHIHTA 586
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 129 bits (312), Expect = 9e-31
Identities = 88/246 (35%), Positives = 129/246 (52%), Gaps = 4/246 (1%)
Frame = +2
Query: 77 EGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSESRFEEIKKEVSSY-I 253
+ FE G +NGQTREHA L LG+ +++V VNK+D +SE RF+EIK VS + I
Sbjct: 290 QNNFERGFLENGQTREHAYLLRALGISEIVVSVNKLDLMS--WSEDRFQEIKNIVSDFLI 347
Query: 254 KKIGYNPAAVAFVPISGWHGDNML--EPSTKMPWFKGWLVERKEGKAEGKCLIEALDAIL 427
K +G+ + V FVPIS G N++ + S W+K G L+ ALD ++
Sbjct: 348 KMVGFKTSNVHFVPISAISGTNLIQKDSSDLYKWYK------------GPTLLSALDQLV 395
Query: 428 PPARPTDKALRLPLQDVYKIGGIGTVPVGRVETGILKPGTVVVFAPANITTEVKSVEMHH 607
PP +P K LRL + DVY+ TV GRVE G ++ V+ + VK+V +
Sbjct: 396 PPEKPYRKPLRLSIDDVYRSPRSVTV-TGRVEAGNVQVNQVLYDVSSQEDAYVKNVIRNS 454
Query: 608 EALQE-AVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPRGAADFTAQVIVLNHPGQISN 784
+ AV GD V + ++ V +LR G + + + NP R F A++ + G I +
Sbjct: 455 DPSSTWAVAGDTVTLQLADIEVNQLRPGDILSNYE-NPVRRVRSFVAEIQTFDIHGPILS 513
Query: 785 GYTPVL 802
G T VL
Sbjct: 514 GSTLVL 519
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 92.7 bits (220), Expect = 1e-19
Identities = 74/248 (29%), Positives = 118/248 (47%), Gaps = 7/248 (2%)
Frame = +2
Query: 113 QTREHALLAFTLGVKQLIVGVNKMDSTEPPYSESRFEEIKKEVSSYIKKIGYNPAAVAFV 292
QTREH LLA +GVKQ++V +NK+D EP E E+++ +S Y
Sbjct: 156 QTREHLLLARQVGVKQIVVYINKVDMVEPDMIELVEMEMRELLSEY-------------- 201
Query: 293 PISGWHGDNM-LEPSTKMPWFKGWLVERKEGKAEGKCLIEALDAILP-PARPTDKALRLP 466
G+ GDN + + + +G E + G L+EA+D+ + P R TD +
Sbjct: 202 ---GFDGDNTPIVSGSALCALEGR--EPEIGLNSITKLMEAVDSYITLPERKTDVPFLMA 256
Query: 467 LQDVYKIGGIGTVPVGRVETGILKPGT--VVVFAPANITTEVKSVEMHHEALQEAVPGDN 640
++DV+ I G GTV GRVE G LK G +V +++ T V +EM + L AV GDN
Sbjct: 257 IEDVFSISGRGTVVTGRVERGTLKKGAEIEIVGYGSHLKTTVTGIEMFKKQLDAAVAGDN 316
Query: 641 VGFNVKNVSVKELRRGYVAGDSKNNPPRGAADFTAQVIVLNHPGQ---ISNGYTPVLDCH 811
G ++++ ++L+RG + P + ++ G+ + Y P L
Sbjct: 317 CGLLLRSIKREQLKRGMIVAQPGTVAPHQKFKASFYILTKEEGGRRTGFVDKYRPQLYSR 376
Query: 812 TAHIACNL 835
T+ + L
Sbjct: 377 TSDVTVEL 384
>SPBC21D10.06c |map4||cell agglutination protein
Map4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 948
Score = 31.1 bits (67), Expect = 0.42
Identities = 21/70 (30%), Positives = 36/70 (51%)
Frame = -3
Query: 678 NSLTDTFLTLKPTLSPGTASCRASWCISTDLTSVVMLAGAKTTTVPGFRIPVSTLPTGTV 499
N+ + + T+ + + ASC S + + +SVV+ + +T TV + VST TGTV
Sbjct: 57 NTTSASVQTIAISQTDNAASCIPSASLLS--SSVVLYSAKETVTVSSYWSLVSTSVTGTV 114
Query: 498 PIPPILYTSC 469
+P +C
Sbjct: 115 YVPYTSSVAC 124
Score = 26.6 bits (56), Expect = 9.0
Identities = 12/34 (35%), Positives = 20/34 (58%)
Frame = +3
Query: 189 PLSHHTVSLDLRKSRRKSLPTSRKLVTIRLPSLS 290
P S +++ +K +LPTS K++T PS+S
Sbjct: 402 PTSFPSLTSSTKKIPSTTLPTSSKMITTTTPSVS 435
>SPAC631.01c |acp2||F-actin capping protein beta subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 268
Score = 26.6 bits (56), Expect = 9.0
Identities = 13/34 (38%), Positives = 19/34 (55%)
Frame = -1
Query: 920 MXASPDLMDLRXSSVDLPVRRSTFSLISANCRLC 819
+ +PDL D+ SSVD P++ +T S LC
Sbjct: 27 LSVAPDLADVLLSSVDQPLKVNTCSESGNQYLLC 60
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 5,294,762
Number of Sequences: 5004
Number of extensions: 105532
Number of successful extensions: 325
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 283
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 316
length of database: 2,362,478
effective HSP length: 76
effective length of database: 1,982,174
effective search space used: 856299168
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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