Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030725E6_G01_e487_13.seq
(1526 letters)
Database: bee
438 sequences; 146,343 total letters
Searching......................................................done
Score E
Sequences producing significant alignments: (bits) Value
AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1al... 514 e-147
X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alp... 492 e-141
AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor 1-a... 425 e-121
EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor 1-a... 257 2e-70
EF013227-1|ABK54581.1| 119|Apis mellifera elongation factor 1-a... 175 1e-45
AF080430-1|AAC28863.2| 208|Apis mellifera ribosomal protein S8 ... 25 1.7
AY686596-1|AAT96374.1| 1946|Apis mellifera Dscam protein. 24 4.0
AB207270-1|BAE72137.1| 429|Apis mellifera broad-complex protein. 23 7.0
AB167961-1|BAD51404.1| 554|Apis mellifera E74 protein. 23 9.2
>AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1alpha
F2 protein.
Length = 461
Score = 514 bits (1267), Expect = e-147
Identities = 240/258 (93%), Positives = 245/258 (94%)
Frame = +2
Query: 80 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSESRFEEIKKEVSSYIKK 259
GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSE+RFEEIKKEVSSYIKK
Sbjct: 121 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKK 180
Query: 260 IGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKGWLVERKEGKAEGKCLIEALDAILPPAR 439
IGYNPAAVAFVPISGWHGDNMLE S+KMPWFKGW VERKEGK EGKCLIEALDAILPP R
Sbjct: 181 IGYNPAAVAFVPISGWHGDNMLEVSSKMPWFKGWTVERKEGKVEGKCLIEALDAILPPTR 240
Query: 440 PTDKALRLPLQDVYKIGGIGTVPVGRVETGILKPGTVVVFAPANITTEVKSVEMHHEALQ 619
PTDKALRLPLQDVYKIGGIGTVPVGRVETG+LKPG VV FAPA +TTEVKSVEMHHEALQ
Sbjct: 241 PTDKALRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFAPAGLTTEVKSVEMHHEALQ 300
Query: 620 EAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPRGAADFTAQVIVLNHPGQISNGYTPV 799
EAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPP+GAADFTAQVIVLNHPGQISNGYTPV
Sbjct: 301 EAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPV 360
Query: 800 LDCHTAHIACNLPKSKRK 853
LDCHTAHIAC K K
Sbjct: 361 LDCHTAHIACKFADIKEK 378
Score = 65.7 bits (153), Expect = 1e-12
Identities = 32/57 (56%), Positives = 41/57 (71%), Gaps = 1/57 (1%)
Frame = +1
Query: 811 HSPH-SLQFAEIKEKVDRRTGKSTEDXLKSIKSGDAXIVNLVXFQPLCVXSXQXFHP 978
H+ H + +FA+IKEK DRR GK+TE+ KSIKSGDA IV LV +P+C + Q F P
Sbjct: 364 HTAHIACKFADIKEKCDRRNGKTTEENPKSIKSGDAAIVMLVPSKPMCAEAFQEFPP 420
Score = 28.3 bits (60), Expect = 0.19
Identities = 18/50 (36%), Positives = 23/50 (46%), Gaps = 2/50 (4%)
Frame = +3
Query: 945 PVCX-VLXXIPPLGRFAVRE*GKRXR-RCIKXXNFXKQXXQVXKLPKKPQ 1088
P+C PPLGRFAVR+ + IK F +V K +K Q
Sbjct: 409 PMCAEAFQEFPPLGRFAVRDMRQTVAVGVIKAVTFKDAAGKVTKAAEKAQ 458
>X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alpha
protein.
Length = 461
Score = 492 bits (1214), Expect = e-141
Identities = 229/258 (88%), Positives = 241/258 (93%)
Frame = +2
Query: 80 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSESRFEEIKKEVSSYIKK 259
GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMD T+PPYSE+RFEEIKKEVSSYIKK
Sbjct: 121 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDMTDPPYSEARFEEIKKEVSSYIKK 180
Query: 260 IGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKGWLVERKEGKAEGKCLIEALDAILPPAR 439
IGYN A+VAFVPISGWHGDNMLEPS K PW+KGW VERK+G A+GK LIEALDAILPP+R
Sbjct: 181 IGYNTASVAFVPISGWHGDNMLEPSPKTPWYKGWKVERKDGNADGKTLIEALDAILPPSR 240
Query: 440 PTDKALRLPLQDVYKIGGIGTVPVGRVETGILKPGTVVVFAPANITTEVKSVEMHHEALQ 619
PTDKALRLPLQDVYKIGGIGTVPVGRVETGILKPG +V FAPA +TTEVKSVEMHHEAL
Sbjct: 241 PTDKALRLPLQDVYKIGGIGTVPVGRVETGILKPGMLVTFAPAALTTEVKSVEMHHEALT 300
Query: 620 EAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPRGAADFTAQVIVLNHPGQISNGYTPV 799
EA+PGDNVGFNVKN+SVKELRRGYVAGDSKN PPRGAADFTAQVIVLNHPGQISNGYTPV
Sbjct: 301 EALPGDNVGFNVKNISVKELRRGYVAGDSKNQPPRGAADFTAQVIVLNHPGQISNGYTPV 360
Query: 800 LDCHTAHIACNLPKSKRK 853
LDCHTAHIAC + K K
Sbjct: 361 LDCHTAHIACKFAEIKEK 378
Score = 68.1 bits (159), Expect = 2e-13
Identities = 34/57 (59%), Positives = 42/57 (73%), Gaps = 1/57 (1%)
Frame = +1
Query: 811 HSPH-SLQFAEIKEKVDRRTGKSTEDXLKSIKSGDAXIVNLVXFQPLCVXSXQXFHP 978
H+ H + +FAEIKEK DRRTGK+TE+ KSIKSGDA IV L +P+CV + Q F P
Sbjct: 364 HTAHIACKFAEIKEKCDRRTGKTTEENPKSIKSGDAAIVMLQPTKPMCVEAFQEFPP 420
Score = 27.5 bits (58), Expect = 0.32
Identities = 18/50 (36%), Positives = 23/50 (46%), Gaps = 2/50 (4%)
Frame = +3
Query: 945 PVCX-VLXXIPPLGRFAVRE*GKRXR-RCIKXXNFXKQXXQVXKLPKKPQ 1088
P+C PPLGRFAVR+ + IK F +V K +K Q
Sbjct: 409 PMCVEAFQEFPPLGRFAVRDMRQTVAVGVIKSVTFKDTQGKVTKAAEKAQ 458
>AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor
1-alpha protein.
Length = 274
Score = 425 bits (1047), Expect = e-121
Identities = 200/211 (94%), Positives = 204/211 (96%)
Frame = +2
Query: 80 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSESRFEEIKKEVSSYIKK 259
GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSE+RFEEIKKEVSSYIKK
Sbjct: 64 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKK 123
Query: 260 IGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKGWLVERKEGKAEGKCLIEALDAILPPAR 439
IGYNPAAVAFVPISGWHGDNMLE S+KMPWFKGW VERKEGK EGKCLIEALDAILPP R
Sbjct: 124 IGYNPAAVAFVPISGWHGDNMLEVSSKMPWFKGWTVERKEGKVEGKCLIEALDAILPPTR 183
Query: 440 PTDKALRLPLQDVYKIGGIGTVPVGRVETGILKPGTVVVFAPANITTEVKSVEMHHEALQ 619
PTDKALRLPLQDVYKIGGIGTVPVGRVETG+LKPG VV FAPA +TTEVKSVEMHHEALQ
Sbjct: 184 PTDKALRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFAPAGLTTEVKSVEMHHEALQ 243
Query: 620 EAVPGDNVGFNVKNVSVKELRRGYVAGDSKN 712
EAVPGDNVGFNVKNVSVKELRRGYVAGDSKN
Sbjct: 244 EAVPGDNVGFNVKNVSVKELRRGYVAGDSKN 274
>EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor
1-alpha protein.
Length = 172
Score = 257 bits (629), Expect = 2e-70
Identities = 119/125 (95%), Positives = 121/125 (96%)
Frame = +2
Query: 80 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSESRFEEIKKEVSSYIKK 259
GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSE+RFEEIKKEVSSYIKK
Sbjct: 48 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKK 107
Query: 260 IGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKGWLVERKEGKAEGKCLIEALDAILPPAR 439
IGYNPAAVAFVPISGWHGDNMLE S+KMPWFKGW VERKEGK EGKCLIEALDAILPP R
Sbjct: 108 IGYNPAAVAFVPISGWHGDNMLEVSSKMPWFKGWTVERKEGKVEGKCLIEALDAILPPTR 167
Query: 440 PTDKA 454
PTDKA
Sbjct: 168 PTDKA 172
>EF013227-1|ABK54581.1| 119|Apis mellifera elongation factor
1-alpha protein.
Length = 119
Score = 175 bits (425), Expect = 1e-45
Identities = 79/89 (88%), Positives = 82/89 (92%)
Frame = +2
Query: 587 KSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPRGAADFTAQVIVLNH 766
KSVEMHHEAL EA+PGDNVGFNVKN+SVKELRRGYVAGDSKN PPRGAADFTAQVIVLNH
Sbjct: 1 KSVEMHHEALTEALPGDNVGFNVKNISVKELRRGYVAGDSKNQPPRGAADFTAQVIVLNH 60
Query: 767 PGQISNGYTPVLDCHTAHIACNLPKSKRK 853
PGQISNGYTPVLDCHTAHIAC + K K
Sbjct: 61 PGQISNGYTPVLDCHTAHIACKFAEIKEK 89
Score = 55.6 bits (128), Expect = 1e-09
Identities = 28/41 (68%), Positives = 33/41 (80%), Gaps = 1/41 (2%)
Frame = +1
Query: 811 HSPH-SLQFAEIKEKVDRRTGKSTEDXLKSIKSGDAXIVNL 930
H+ H + +FAEIKEK DRRTGK+TE+ KSIKSGDA IV L
Sbjct: 75 HTAHIACKFAEIKEKCDRRTGKTTEENPKSIKSGDAAIVML 115
>AF080430-1|AAC28863.2| 208|Apis mellifera ribosomal protein S8
protein.
Length = 208
Score = 25.0 bits (52), Expect = 1.7
Identities = 14/36 (38%), Positives = 19/36 (52%), Gaps = 1/36 (2%)
Frame = +3
Query: 279 PSLSYPFLAGTEITC-WSRPPKCHGSRDGWLSVKKE 383
P+L F G + C SRP +C G DG++ KE
Sbjct: 160 PALEEQFATGRVLACISSRPGQC-GREDGYILEGKE 194
>AY686596-1|AAT96374.1| 1946|Apis mellifera Dscam protein.
Length = 1946
Score = 23.8 bits (49), Expect = 4.0
Identities = 12/37 (32%), Positives = 17/37 (45%)
Frame = +1
Query: 709 KQPTQGSCRLHSASHCAKSPRSNIKRIHTCIGLPHSP 819
K TQ S + + + P S +R HT G+P P
Sbjct: 1048 KTYTQYSVVVQAFNKVGSGPMSEERRQHTAEGVPEQP 1084
>AB207270-1|BAE72137.1| 429|Apis mellifera broad-complex protein.
Length = 429
Score = 23.0 bits (47), Expect = 7.0
Identities = 9/15 (60%), Positives = 10/15 (66%)
Frame = -2
Query: 1021 RRXRLPHSRTAKRPR 977
RR PH TAK+PR
Sbjct: 181 RRTPDPHDETAKKPR 195
>AB167961-1|BAD51404.1| 554|Apis mellifera E74 protein.
Length = 554
Score = 22.6 bits (46), Expect = 9.2
Identities = 8/17 (47%), Positives = 12/17 (70%)
Frame = -2
Query: 283 DGSRIVTNFLDVGRDFL 233
DG R+V F+DV +D +
Sbjct: 531 DGQRLVYQFVDVPKDII 547
Database: bee
Posted date: Oct 23, 2007 1:17 PM
Number of letters in database: 146,343
Number of sequences in database: 438
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 377,186
Number of Sequences: 438
Number of extensions: 7853
Number of successful extensions: 27
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 20
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 27
length of database: 146,343
effective HSP length: 61
effective length of database: 119,625
effective search space used: 53472375
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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