BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= 030725E6_F11_e566_11.seq (1546 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 308 1e-84 SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 150 4e-37 SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 137 3e-33 SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 118 2e-27 SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 95 2e-20 SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 81 4e-16 SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 75 3e-14 SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 70 8e-13 SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb... 44 4e-05 SPBC18E5.09c |||sequence orphan|Schizosaccharomyces pombe|chr 2|... 28 3.0 SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 28 4.0 SPBC21B10.07 |||glycosyl hydrolase family 16|Schizosaccharomyces... 27 6.9 SPBP4H10.15 |||aconitate hydratase|Schizosaccharomyces pombe|chr... 27 9.2 >SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2|||Manual Length = 375 Score = 308 bits (756), Expect = 1e-84 Identities = 143/163 (87%), Positives = 151/163 (92%) Frame = +3 Query: 87 RXKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMEAC 266 + KLCYVALDFEQE+ TAA SSSLEKSYELPDGQVITIGNERFR PEALFQPS LG+E Sbjct: 213 KEKLCYVALDFEQELQTAAQSSSLEKSYELPDGQVITIGNERFRAPEALFQPSALGLENA 272 Query: 267 GIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAP 446 GIHE TYNSIMKCDVDIRKDLY N V+SGGTTMYPGIADRMQKEI ALAPS+MK+KI+AP Sbjct: 273 GIHEATYNSIMKCDVDIRKDLYGNVVMSGGTTMYPGIADRMQKEIQALAPSSMKVKIVAP 332 Query: 447 PERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF 575 PERKYSVWIGGSILASLSTFQQMWISKQEYDESGP IV+RKCF Sbjct: 333 PERKYSVWIGGSILASLSTFQQMWISKQEYDESGPGIVYRKCF 375 >SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 150 bits (364), Expect = 4e-37 Identities = 69/163 (42%), Positives = 104/163 (63%) Frame = +3 Query: 87 RXKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMEAC 266 + K CYVA DF E+ + +Y+LPD Q IT+G E F PE LF P +G EA Sbjct: 218 KEKCCYVASDFRSEIESWTEHKPQIHTYQLPDNQTITLGTECFSAPEVLFNPEMMGSEAS 277 Query: 267 GIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAP 446 G+H + SI+ D+D+R LY+N VLSGG+T+ G +R E+ A++ ++KI A Sbjct: 278 GLHIQLFKSILLSDIDLRSTLYSNIVLSGGSTLLRGFGERFISELRAISGKKNQVKIYAS 337 Query: 447 PERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF 575 PER ++ W+GGSILASLSTF+++ I+ +EY ++ +++ R+ F Sbjct: 338 PERMHNAWLGGSILASLSTFRRLLITSEEY-KNDQNVIFRRRF 379 >SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 137 bits (332), Expect = 3e-33 Identities = 70/166 (42%), Positives = 106/166 (63%), Gaps = 12/166 (7%) Frame = +3 Query: 87 RXKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMEAC 266 + KLCYV+ D E + + ++ L ++Y LPDG+VI +G+ER+ CPE LFQP +G E Sbjct: 213 KEKLCYVSYDLELDHKLSEETTVLMRNYTLPDGRVIKVGSERYECPECLFQPHLVGSEQP 272 Query: 267 GIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITAL--------APST 422 G+ E +++I DVDIRK LY VLSGG++MY G+ R++KEI L P+ Sbjct: 273 GLSEFIFDTIQAADVDIRKYLYRAIVLSGGSSMYAGLPSRLEKEIKQLWFERVLHGDPAR 332 Query: 423 M---KIKIIAPPERKYSVWIGGSILAS-LSTFQQMWISKQEYDESG 548 + K+KI P R+++V+IGG++LA ++ MW+SK E++E G Sbjct: 333 LPNFKVKIEDAPRRRHAVFIGGAVLADIMAQNDHMWVSKAEWEEYG 378 >SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 430 Score = 118 bits (285), Expect = 2e-27 Identities = 63/148 (42%), Positives = 84/148 (56%), Gaps = 11/148 (7%) Frame = +3 Query: 138 AASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMEAC---------GIHETTYN 290 A +SS K +E PDG G ERFR E LF PSF + G+HE Y Sbjct: 271 AKASSRNPKPFEFPDGVTHKFGQERFRISEILFNPSFSASRSAETTPPQGSVGLHELVYQ 330 Query: 291 SIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAP-STMKIKII-APPERKYS 464 SI+ CD ++R L N V++GGT++ PG+++R+Q E+ LA S + + + Sbjct: 331 SILACDSELRSPLLNNIVVTGGTSLIPGLSERLQAEVQRLATGSRINVHTAETASATSNA 390 Query: 465 VWIGGSILASLSTFQQMWISKQEYDESG 548 VW GGSILASL FQ +W+SKQEYDE G Sbjct: 391 VWFGGSILASLDNFQHLWVSKQEYDEVG 418 >SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 95.1 bits (226), Expect = 2e-20 Identities = 47/108 (43%), Positives = 71/108 (65%), Gaps = 6/108 (5%) Frame = +3 Query: 267 GIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAP 446 GI + N I +CDVDIR L N ++ GGT++ G + R+Q E++ L P + ++KI A Sbjct: 326 GIPQLFQNCISECDVDIRASLLNNVIVCGGTSLMQGFSLRLQNELSKLYPGS-RLKIHAS 384 Query: 447 P---ERKYSVWIGGSILASLSTFQQMWISKQEYDESGP---SIVHRKC 572 ER Y+ W+GGSIL+SL TF Q+WIS+QEY+E G +++ ++C Sbjct: 385 GHVVERSYASWLGGSILSSLGTFHQLWISRQEYEEHGSDRLALIEKRC 432 >SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces pombe|chr 3|||Manual Length = 401 Score = 81.0 bits (191), Expect = 4e-16 Identities = 52/169 (30%), Positives = 79/169 (46%), Gaps = 2/169 (1%) Frame = +3 Query: 69 IRHEXSRXKL--CYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQP 242 I HE R KL CY D+ +T + + + QV+ + NERF PE LF P Sbjct: 235 ICHEKPRSKLEICYALPDY----STGKHGYIVRDINQKIEQQVLNLSNERFMIPELLFSP 290 Query: 243 SFLGMEACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPST 422 S + + GI E S+ +I+ L N V GG +PG R+ E+ +LAP+ Sbjct: 291 SDIEIREAGIPEAVMQSVTHFPENIQAMLLENVVTIGGNCKFPGFHKRLSSELRSLAPAN 350 Query: 423 MKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRK 569 ++K+ P + W S + L + I++ EY E G +I+ RK Sbjct: 351 WEVKVFEPSDPICFPWKKASHM-PLEHWNANKITRSEYSEHGANIMTRK 398 >SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 74.9 bits (176), Expect = 3e-14 Identities = 51/182 (28%), Positives = 82/182 (45%), Gaps = 19/182 (10%) Frame = +3 Query: 78 EXSRXKLCYVALDFEQEMATAASSSS--LEKSYELPDGQVITI--GNERFRCPEALFQPS 245 E + + CYV D +E + L+ + E G TI G ERF PE F P Sbjct: 238 ERIKEECCYVCPDIVKEFSRFDREPDRYLKYASESITGHSTTIDVGFERFLAPEIFFNPE 297 Query: 246 FLGME-ACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPST 422 + + E N + +D+RK LY N VLSGG+T++ +R+Q+++ + Sbjct: 298 IASSDFLTPLPELVDNVVQSSPIDVRKGLYKNIVLSGGSTLFKNFGNRLQRDLKRIVDER 357 Query: 423 --------------MKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIV 560 + + +I+ ++ +VW GGS+LA F +K +Y+E G SI Sbjct: 358 IHRSEMLSGAKSGGVDVNVISHKRQRNAVWFGGSLLAQTPEFGSYCHTKADYEEYGASIA 417 Query: 561 HR 566 R Sbjct: 418 RR 419 >SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 721 Score = 70.1 bits (164), Expect = 8e-13 Identities = 31/118 (26%), Positives = 62/118 (52%) Frame = +3 Query: 207 ERFRCPEALFQPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADR 386 E+ R PE +F PS +G++ GI E + + + ++ ++ L +N +++GG PG+ R Sbjct: 594 EQIRVPEVIFSPSIVGIDQAGILEIMRSILQRHSLEEQQKLVSNVLITGGLGSLPGMETR 653 Query: 387 MQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIV 560 +++E+T++ P I + W G S + F+ ++++EY E GP + Sbjct: 654 IKRELTSIMPVGSSINVFRASNPLLDAWKGASEWSVTEKFKAAKVTREEYLEKGPEYI 711 >SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pombe|chr 1|||Manual Length = 620 Score = 44.4 bits (100), Expect = 4e-05 Identities = 20/86 (23%), Positives = 42/86 (48%), Gaps = 3/86 (3%) Frame = +3 Query: 321 KDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPP---ERKYSVWIGGSILA 491 K+LY++ ++ GG +PG A +++ I + + I +I PP + ++ W G I Sbjct: 527 KNLYSSILIVGGAGQFPGFAHLLEERIHSKRANIPTISVIPPPRSMDAQFVAWKGACIYN 586 Query: 492 SLSTFQQMWISKQEYDESGPSIVHRK 569 + ++WI ++ G ++ K Sbjct: 587 RIRIVSELWIKNSDWKMLGSRVLQYK 612 >SPBC18E5.09c |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 128 Score = 28.3 bits (60), Expect = 3.0 Identities = 18/66 (27%), Positives = 28/66 (42%), Gaps = 1/66 (1%) Frame = +3 Query: 30 ALXTSGXPPGLQXIRHEXSRXKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNE 209 AL T+ P +E CYV+ +E + + SL SY +PD + I + Sbjct: 16 ALTTNFSFPSYDLSFNETEHGVFCYVSRPLTKERSCSHPYISLGSSYGIPDAENIEYPRD 75 Query: 210 -RFRCP 224 R+ P Sbjct: 76 ARYHSP 81 >SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosaccharomyces pombe|chr 1|||Manual Length = 523 Score = 27.9 bits (59), Expect = 4.0 Identities = 12/37 (32%), Positives = 24/37 (64%), Gaps = 3/37 (8%) Frame = +3 Query: 468 WIGGSILASLSTFQQM---WISKQEYDESGPSIVHRK 569 ++GGSI+A S + + +++ +EY + GP+ +H K Sbjct: 486 FLGGSIVAKTSFNESVSSHYVTLEEYAQHGPTAIHTK 522 >SPBC21B10.07 |||glycosyl hydrolase family 16|Schizosaccharomyces pombe|chr 2|||Manual Length = 419 Score = 27.1 bits (57), Expect = 6.9 Identities = 19/59 (32%), Positives = 25/59 (42%) Frame = -3 Query: 386 TVGNTRVHGGTTRQYCVGVQVFTDVNVTLHDGVVCRLVDATSFHTQERGLEESLWATEP 210 T+G+ +GG GV V T VTLH G C + D T L+ + W P Sbjct: 227 TLGDDWPNGGEI-DIVEGVNVGTSNQVTLHTGDGCEMEDIKRVMT-GTALQTNCWVDAP 283 >SPBP4H10.15 |||aconitate hydratase|Schizosaccharomyces pombe|chr 2|||Manual Length = 905 Score = 26.6 bits (56), Expect = 9.2 Identities = 10/22 (45%), Positives = 12/22 (54%) Frame = -3 Query: 419 GWSEGCDFLLHTVGNTRVHGGT 354 GW+ D +LH G V GGT Sbjct: 240 GWTTPKDLILHLAGKLTVRGGT 261 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 4,265,502 Number of Sequences: 5004 Number of extensions: 75563 Number of successful extensions: 216 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 198 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 209 length of database: 2,362,478 effective HSP length: 76 effective length of database: 1,982,174 effective search space used: 868192212 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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