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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 030725E6_F11_e566_11.seq
         (1546 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||...   308   1e-84
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb...   150   4e-37
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s...   137   3e-33
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc...   118   2e-27
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy...    95   2e-20
SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po...    81   4e-16
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce...    75   3e-14
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe...    70   8e-13
SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb...    44   4e-05
SPBC18E5.09c |||sequence orphan|Schizosaccharomyces pombe|chr 2|...    28   3.0  
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa...    28   4.0  
SPBC21B10.07 |||glycosyl hydrolase family 16|Schizosaccharomyces...    27   6.9  
SPBP4H10.15 |||aconitate hydratase|Schizosaccharomyces pombe|chr...    27   9.2  

>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 375

 Score =  308 bits (756), Expect = 1e-84
 Identities = 143/163 (87%), Positives = 151/163 (92%)
 Frame = +3

Query: 87  RXKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMEAC 266
           + KLCYVALDFEQE+ TAA SSSLEKSYELPDGQVITIGNERFR PEALFQPS LG+E  
Sbjct: 213 KEKLCYVALDFEQELQTAAQSSSLEKSYELPDGQVITIGNERFRAPEALFQPSALGLENA 272

Query: 267 GIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAP 446
           GIHE TYNSIMKCDVDIRKDLY N V+SGGTTMYPGIADRMQKEI ALAPS+MK+KI+AP
Sbjct: 273 GIHEATYNSIMKCDVDIRKDLYGNVVMSGGTTMYPGIADRMQKEIQALAPSSMKVKIVAP 332

Query: 447 PERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF 575
           PERKYSVWIGGSILASLSTFQQMWISKQEYDESGP IV+RKCF
Sbjct: 333 PERKYSVWIGGSILASLSTFQQMWISKQEYDESGPGIVYRKCF 375


>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 379

 Score =  150 bits (364), Expect = 4e-37
 Identities = 69/163 (42%), Positives = 104/163 (63%)
 Frame = +3

Query: 87  RXKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMEAC 266
           + K CYVA DF  E+ +         +Y+LPD Q IT+G E F  PE LF P  +G EA 
Sbjct: 218 KEKCCYVASDFRSEIESWTEHKPQIHTYQLPDNQTITLGTECFSAPEVLFNPEMMGSEAS 277

Query: 267 GIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAP 446
           G+H   + SI+  D+D+R  LY+N VLSGG+T+  G  +R   E+ A++    ++KI A 
Sbjct: 278 GLHIQLFKSILLSDIDLRSTLYSNIVLSGGSTLLRGFGERFISELRAISGKKNQVKIYAS 337

Query: 447 PERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF 575
           PER ++ W+GGSILASLSTF+++ I+ +EY ++  +++ R+ F
Sbjct: 338 PERMHNAWLGGSILASLSTFRRLLITSEEY-KNDQNVIFRRRF 379


>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
           subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 390

 Score =  137 bits (332), Expect = 3e-33
 Identities = 70/166 (42%), Positives = 106/166 (63%), Gaps = 12/166 (7%)
 Frame = +3

Query: 87  RXKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMEAC 266
           + KLCYV+ D E +   +  ++ L ++Y LPDG+VI +G+ER+ CPE LFQP  +G E  
Sbjct: 213 KEKLCYVSYDLELDHKLSEETTVLMRNYTLPDGRVIKVGSERYECPECLFQPHLVGSEQP 272

Query: 267 GIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITAL--------APST 422
           G+ E  +++I   DVDIRK LY   VLSGG++MY G+  R++KEI  L         P+ 
Sbjct: 273 GLSEFIFDTIQAADVDIRKYLYRAIVLSGGSSMYAGLPSRLEKEIKQLWFERVLHGDPAR 332

Query: 423 M---KIKIIAPPERKYSVWIGGSILAS-LSTFQQMWISKQEYDESG 548
           +   K+KI   P R+++V+IGG++LA  ++    MW+SK E++E G
Sbjct: 333 LPNFKVKIEDAPRRRHAVFIGGAVLADIMAQNDHMWVSKAEWEEYG 378


>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
           Arp42|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 430

 Score =  118 bits (285), Expect = 2e-27
 Identities = 63/148 (42%), Positives = 84/148 (56%), Gaps = 11/148 (7%)
 Frame = +3

Query: 138 AASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMEAC---------GIHETTYN 290
           A +SS   K +E PDG     G ERFR  E LF PSF    +          G+HE  Y 
Sbjct: 271 AKASSRNPKPFEFPDGVTHKFGQERFRISEILFNPSFSASRSAETTPPQGSVGLHELVYQ 330

Query: 291 SIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAP-STMKIKII-APPERKYS 464
           SI+ CD ++R  L  N V++GGT++ PG+++R+Q E+  LA  S + +           +
Sbjct: 331 SILACDSELRSPLLNNIVVTGGTSLIPGLSERLQAEVQRLATGSRINVHTAETASATSNA 390

Query: 465 VWIGGSILASLSTFQQMWISKQEYDESG 548
           VW GGSILASL  FQ +W+SKQEYDE G
Sbjct: 391 VWFGGSILASLDNFQHLWVSKQEYDEVG 418


>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 433

 Score = 95.1 bits (226), Expect = 2e-20
 Identities = 47/108 (43%), Positives = 71/108 (65%), Gaps = 6/108 (5%)
 Frame = +3

Query: 267 GIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAP 446
           GI +   N I +CDVDIR  L  N ++ GGT++  G + R+Q E++ L P + ++KI A 
Sbjct: 326 GIPQLFQNCISECDVDIRASLLNNVIVCGGTSLMQGFSLRLQNELSKLYPGS-RLKIHAS 384

Query: 447 P---ERKYSVWIGGSILASLSTFQQMWISKQEYDESGP---SIVHRKC 572
               ER Y+ W+GGSIL+SL TF Q+WIS+QEY+E G    +++ ++C
Sbjct: 385 GHVVERSYASWLGGSILSSLGTFHQLWISRQEYEEHGSDRLALIEKRC 432


>SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 401

 Score = 81.0 bits (191), Expect = 4e-16
 Identities = 52/169 (30%), Positives = 79/169 (46%), Gaps = 2/169 (1%)
 Frame = +3

Query: 69  IRHEXSRXKL--CYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQP 242
           I HE  R KL  CY   D+    +T      +    +  + QV+ + NERF  PE LF P
Sbjct: 235 ICHEKPRSKLEICYALPDY----STGKHGYIVRDINQKIEQQVLNLSNERFMIPELLFSP 290

Query: 243 SFLGMEACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPST 422
           S + +   GI E    S+     +I+  L  N V  GG   +PG   R+  E+ +LAP+ 
Sbjct: 291 SDIEIREAGIPEAVMQSVTHFPENIQAMLLENVVTIGGNCKFPGFHKRLSSELRSLAPAN 350

Query: 423 MKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRK 569
            ++K+  P +     W   S +  L  +    I++ EY E G +I+ RK
Sbjct: 351 WEVKVFEPSDPICFPWKKASHM-PLEHWNANKITRSEYSEHGANIMTRK 398


>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 427

 Score = 74.9 bits (176), Expect = 3e-14
 Identities = 51/182 (28%), Positives = 82/182 (45%), Gaps = 19/182 (10%)
 Frame = +3

Query: 78  EXSRXKLCYVALDFEQEMATAASSSS--LEKSYELPDGQVITI--GNERFRCPEALFQPS 245
           E  + + CYV  D  +E +         L+ + E   G   TI  G ERF  PE  F P 
Sbjct: 238 ERIKEECCYVCPDIVKEFSRFDREPDRYLKYASESITGHSTTIDVGFERFLAPEIFFNPE 297

Query: 246 FLGME-ACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPST 422
               +    + E   N +    +D+RK LY N VLSGG+T++    +R+Q+++  +    
Sbjct: 298 IASSDFLTPLPELVDNVVQSSPIDVRKGLYKNIVLSGGSTLFKNFGNRLQRDLKRIVDER 357

Query: 423 --------------MKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIV 560
                         + + +I+   ++ +VW GGS+LA    F     +K +Y+E G SI 
Sbjct: 358 IHRSEMLSGAKSGGVDVNVISHKRQRNAVWFGGSLLAQTPEFGSYCHTKADYEEYGASIA 417

Query: 561 HR 566
            R
Sbjct: 418 RR 419


>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 721

 Score = 70.1 bits (164), Expect = 8e-13
 Identities = 31/118 (26%), Positives = 62/118 (52%)
 Frame = +3

Query: 207 ERFRCPEALFQPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADR 386
           E+ R PE +F PS +G++  GI E   + + +  ++ ++ L +N +++GG    PG+  R
Sbjct: 594 EQIRVPEVIFSPSIVGIDQAGILEIMRSILQRHSLEEQQKLVSNVLITGGLGSLPGMETR 653

Query: 387 MQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIV 560
           +++E+T++ P    I +          W G S  +    F+   ++++EY E GP  +
Sbjct: 654 IKRELTSIMPVGSSINVFRASNPLLDAWKGASEWSVTEKFKAAKVTREEYLEKGPEYI 711


>SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 620

 Score = 44.4 bits (100), Expect = 4e-05
 Identities = 20/86 (23%), Positives = 42/86 (48%), Gaps = 3/86 (3%)
 Frame = +3

Query: 321 KDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPP---ERKYSVWIGGSILA 491
           K+LY++ ++ GG   +PG A  +++ I +   +   I +I PP   + ++  W G  I  
Sbjct: 527 KNLYSSILIVGGAGQFPGFAHLLEERIHSKRANIPTISVIPPPRSMDAQFVAWKGACIYN 586

Query: 492 SLSTFQQMWISKQEYDESGPSIVHRK 569
            +    ++WI   ++   G  ++  K
Sbjct: 587 RIRIVSELWIKNSDWKMLGSRVLQYK 612


>SPBC18E5.09c |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 128

 Score = 28.3 bits (60), Expect = 3.0
 Identities = 18/66 (27%), Positives = 28/66 (42%), Gaps = 1/66 (1%)
 Frame = +3

Query: 30  ALXTSGXPPGLQXIRHEXSRXKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNE 209
           AL T+   P      +E      CYV+    +E + +    SL  SY +PD + I    +
Sbjct: 16  ALTTNFSFPSYDLSFNETEHGVFCYVSRPLTKERSCSHPYISLGSSYGIPDAENIEYPRD 75

Query: 210 -RFRCP 224
            R+  P
Sbjct: 76  ARYHSP 81


>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
           Arp9|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 523

 Score = 27.9 bits (59), Expect = 4.0
 Identities = 12/37 (32%), Positives = 24/37 (64%), Gaps = 3/37 (8%)
 Frame = +3

Query: 468 WIGGSILASLSTFQQM---WISKQEYDESGPSIVHRK 569
           ++GGSI+A  S  + +   +++ +EY + GP+ +H K
Sbjct: 486 FLGGSIVAKTSFNESVSSHYVTLEEYAQHGPTAIHTK 522


>SPBC21B10.07 |||glycosyl hydrolase family 16|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 419

 Score = 27.1 bits (57), Expect = 6.9
 Identities = 19/59 (32%), Positives = 25/59 (42%)
 Frame = -3

Query: 386 TVGNTRVHGGTTRQYCVGVQVFTDVNVTLHDGVVCRLVDATSFHTQERGLEESLWATEP 210
           T+G+   +GG       GV V T   VTLH G  C + D     T    L+ + W   P
Sbjct: 227 TLGDDWPNGGEI-DIVEGVNVGTSNQVTLHTGDGCEMEDIKRVMT-GTALQTNCWVDAP 283


>SPBP4H10.15 |||aconitate hydratase|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 905

 Score = 26.6 bits (56), Expect = 9.2
 Identities = 10/22 (45%), Positives = 12/22 (54%)
 Frame = -3

Query: 419 GWSEGCDFLLHTVGNTRVHGGT 354
           GW+   D +LH  G   V GGT
Sbjct: 240 GWTTPKDLILHLAGKLTVRGGT 261


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 4,265,502
Number of Sequences: 5004
Number of extensions: 75563
Number of successful extensions: 216
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 198
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 209
length of database: 2,362,478
effective HSP length: 76
effective length of database: 1,982,174
effective search space used: 868192212
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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