BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= 030725E6_F10_e558_12.seq (1539 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBP22H7.08 |rps1002|rps10-2, rps10B|40S ribosomal protein S10|S... 78 3e-15 SPAC31G5.17c |rps1001|rps10-1|40S ribosomal protein S10|Schizosa... 74 5e-14 SPBC56F2.07c |||AAA family ATPase, unknown biological role|Schiz... 28 3.0 SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 27 9.1 SPCC1223.01 ||SPCC285.18|ubiquitin-protein ligase E3 |Schizosacc... 27 9.1 >SPBP22H7.08 |rps1002|rps10-2, rps10B|40S ribosomal protein S10|Schizosaccharomyces pombe|chr 2|||Manual Length = 147 Score = 78.2 bits (184), Expect = 3e-15 Identities = 45/90 (50%), Positives = 54/90 (60%), Gaps = 1/90 (1%) Frame = +3 Query: 129 FYWYLTNEGIEYLRIFLHLPPEIVPATLKRSVRTETVRRGAVGRPDAPARTAEDRSAYRR 308 FY+ LTNEG+EYLR +LHLP E+VPAT KR VR R GRP+ R++ + YRR Sbjct: 64 FYYTLTNEGVEYLREYLHLPAEVVPATHKRQVRPAAPR---AGRPEPRERSSAADAGYRR 120 Query: 309 APPAGAPHDKKAD-VGPGSADVEFRGGFGR 395 A +KK D PG FRGGFGR Sbjct: 121 A-------EKKDDGAAPGGFAPSFRGGFGR 143 >SPAC31G5.17c |rps1001|rps10-1|40S ribosomal protein S10|Schizosaccharomyces pombe|chr 1|||Manual Length = 144 Score = 74.1 bits (174), Expect = 5e-14 Identities = 43/89 (48%), Positives = 50/89 (56%) Frame = +3 Query: 129 FYWYLTNEGIEYLRIFLHLPPEIVPATLKRSVRTETVRRGAVGRPDAPARTAEDRSAYRR 308 FY+ LTNEG+EYLR +LHLP E+VPAT KR VR R GRP+ R + D + YRR Sbjct: 64 FYYTLTNEGVEYLREYLHLPAEVVPATHKRQVRPTAPR---AGRPEPRERASAD-AGYRR 119 Query: 309 APPAGAPHDKKADVGPGSADVEFRGGFGR 395 A K P FRGGFGR Sbjct: 120 A------EKKDEGAAPSGFAPSFRGGFGR 142 >SPBC56F2.07c |||AAA family ATPase, unknown biological role|Schizosaccharomyces pombe|chr 2|||Manual Length = 809 Score = 28.3 bits (60), Expect = 3.0 Identities = 12/34 (35%), Positives = 21/34 (61%) Frame = +1 Query: 250 QLAGLMLQHEQLKIDQLIGAHHQLELLMIRRLMS 351 Q AGL+ HE L+ ++ AH + LL +R+ ++ Sbjct: 758 QEAGLIAMHEDLEAKEICQAHFKTALLALRKAIT 791 >SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1275 Score = 26.6 bits (56), Expect = 9.1 Identities = 19/63 (30%), Positives = 31/63 (49%), Gaps = 5/63 (7%) Frame = +3 Query: 474 PIRPIVSRITIHWPSFYNVVTGKTLALPNLIALQ-----HIPLSPAGVIAKRPAPIALPN 638 P P+ S ++ H + + +L N I+L ++PLSP A+ P+PI L + Sbjct: 172 PRPPLPSSVSSHSSPYSTTSSTSLYSLYNDISLSCSPEPYLPLSPTRSPARTPSPIRLYS 231 Query: 639 SCA 647 S A Sbjct: 232 SDA 234 >SPCC1223.01 ||SPCC285.18|ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 732 Score = 26.6 bits (56), Expect = 9.1 Identities = 12/25 (48%), Positives = 15/25 (60%) Frame = +2 Query: 581 PPFASWRNSEEARTDRPSQQLRSLN 655 PP AS RN E + PS+Q S+N Sbjct: 353 PPGASGRNRRERTSSTPSEQSTSVN 377 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 4,528,577 Number of Sequences: 5004 Number of extensions: 82296 Number of successful extensions: 184 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 171 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 182 length of database: 2,362,478 effective HSP length: 76 effective length of database: 1,982,174 effective search space used: 864227864 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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