Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030725E6_F07_e534_11.seq
(1510 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g33140.1 68414.m04093 60S ribosomal protein L9 (RPL90A/C) sim... 218 9e-57
At1g33120.1 68414.m04090 60S ribosomal protein L9 (RPL90B) simil... 218 9e-57
At4g10450.1 68417.m01717 60S ribosomal protein L9 (RPL90D) ribos... 216 4e-56
At5g56000.1 68418.m06988 heat shock protein 81-4 (HSP81-4) nearl... 61 2e-09
At5g52640.1 68418.m06535 heat shock protein 81-1 (HSP81-1) / hea... 61 2e-09
At5g56030.1 68418.m06991 heat shock protein 81-2 (HSP81-2) nearl... 58 2e-08
At5g56010.1 68418.m06989 heat shock protein, putative strong sim... 58 2e-08
At4g24190.2 68417.m03473 shepherd protein (SHD) / clavata format... 42 8e-04
At4g24190.1 68417.m03472 shepherd protein (SHD) / clavata format... 42 8e-04
At3g07770.1 68416.m00947 heat shock protein-related strong simil... 40 0.006
At1g05190.1 68414.m00523 ribosomal protein L6 family protein Sim... 39 0.010
At2g04030.2 68415.m00372 heat shock protein, putative strong sim... 32 0.86
At2g04030.1 68415.m00371 heat shock protein, putative strong sim... 32 0.86
At3g58060.1 68416.m06472 cation efflux family protein / metal to... 31 2.6
At1g76660.1 68414.m08920 expressed protein 30 4.6
>At1g33140.1 68414.m04093 60S ribosomal protein L9 (RPL90A/C)
similar to RIBOSOMAL PROTEIN L9 GB:P49209 from
[Arabidopsis thaliana]
Length = 194
Score = 218 bits (532), Expect = 9e-57
Identities = 101/194 (52%), Positives = 144/194 (74%), Gaps = 5/194 (2%)
Frame = +2
Query: 104 MKQIVANQKVKIPESLTVHVKSRLVTVKGPRGVLKRNFKHLAVDIRMVNP-----RQLKV 268
MK I++++ + IP+S+T+ V ++++ V+GPRG L R+FKHL +D +++ ++LK+
Sbjct: 1 MKTILSSETMDIPDSVTIKVHAKVIEVEGPRGKLVRDFKHLNLDFQLIKDPETGKKKLKI 60
Query: 269 EKWFGSKKELAAVRTVCSHVENMIKGVTKGFQYKMRAVYAHFPINCVTTEGNTIIEIRNF 448
+ WFG++K A++RT SHV+N+I GVT+GF+YKMR VYAHFPIN IEIRNF
Sbjct: 61 DSWFGTRKTSASIRTALSHVDNLISGVTRGFRYKMRFVYAHFPINASIGGDGKSIEIRNF 120
Query: 449 LGEKYIRRVKMAPGVTVVNSPKQKDELIIEGNSLEDVSSSAALIQQSTTVKNKDIRKFLD 628
LGEK +R+V+M GVT+V S K KDE++++GN +E VS S ALI Q VK KDIRKFLD
Sbjct: 121 LGEKKVRKVEMLDGVTIVRSEKVKDEIVLDGNDIELVSRSCALINQKCHVKKKDIRKFLD 180
Query: 629 GLYVSEKTTVVADE 670
G+YVSEK+ +V +E
Sbjct: 181 GIYVSEKSKIVEEE 194
>At1g33120.1 68414.m04090 60S ribosomal protein L9 (RPL90B) similar
to RIBOSOMAL PROTEIN L9 GB:P49209 from [Arabidopsis
thaliana]
Length = 194
Score = 218 bits (532), Expect = 9e-57
Identities = 101/194 (52%), Positives = 144/194 (74%), Gaps = 5/194 (2%)
Frame = +2
Query: 104 MKQIVANQKVKIPESLTVHVKSRLVTVKGPRGVLKRNFKHLAVDIRMVNP-----RQLKV 268
MK I++++ + IP+S+T+ V ++++ V+GPRG L R+FKHL +D +++ ++LK+
Sbjct: 1 MKTILSSETMDIPDSVTIKVHAKVIEVEGPRGKLVRDFKHLNLDFQLIKDPETGKKKLKI 60
Query: 269 EKWFGSKKELAAVRTVCSHVENMIKGVTKGFQYKMRAVYAHFPINCVTTEGNTIIEIRNF 448
+ WFG++K A++RT SHV+N+I GVT+GF+YKMR VYAHFPIN IEIRNF
Sbjct: 61 DSWFGTRKTSASIRTALSHVDNLISGVTRGFRYKMRFVYAHFPINASIGGDGKSIEIRNF 120
Query: 449 LGEKYIRRVKMAPGVTVVNSPKQKDELIIEGNSLEDVSSSAALIQQSTTVKNKDIRKFLD 628
LGEK +R+V+M GVT+V S K KDE++++GN +E VS S ALI Q VK KDIRKFLD
Sbjct: 121 LGEKKVRKVEMLDGVTIVRSEKVKDEIVLDGNDIELVSRSCALINQKCHVKKKDIRKFLD 180
Query: 629 GLYVSEKTTVVADE 670
G+YVSEK+ +V +E
Sbjct: 181 GIYVSEKSKIVEEE 194
>At4g10450.1 68417.m01717 60S ribosomal protein L9 (RPL90D)
ribosomal protein L9, cytosolic - garden pea,
PIR2:S19978
Length = 194
Score = 216 bits (527), Expect = 4e-56
Identities = 102/194 (52%), Positives = 139/194 (71%), Gaps = 5/194 (2%)
Frame = +2
Query: 104 MKQIVANQKVKIPESLTVHVKSRLVTVKGPRGVLKRNFKHLAVDIRMVNP-----RQLKV 268
MK I++++ + IP+ + + V ++++ V+GPRG L R+FKHL +D +++ RQLK+
Sbjct: 1 MKTILSSETMDIPDGVAIKVNAKVIEVEGPRGKLTRDFKHLNLDFQLIKDQVTGKRQLKI 60
Query: 269 EKWFGSKKELAAVRTVCSHVENMIKGVTKGFQYKMRAVYAHFPINCVTTEGNTIIEIRNF 448
+ WFGS+K A++RT SHV+N+I GVT+GF Y+MR VYAHFPIN N IEIRNF
Sbjct: 61 DSWFGSRKTSASIRTALSHVDNLIAGVTQGFLYRMRFVYAHFPINASIDGNNKSIEIRNF 120
Query: 449 LGEKYIRRVKMAPGVTVVNSPKQKDELIIEGNSLEDVSSSAALIQQSTTVKNKDIRKFLD 628
LGEK +R+V+M GV +V S K KDE+I+EGN +E VS S ALI Q VK KDIRKFLD
Sbjct: 121 LGEKKVRKVEMLDGVKIVRSEKVKDEIILEGNDIELVSRSCALINQKCHVKKKDIRKFLD 180
Query: 629 GLYVSEKTTVVADE 670
G+YVSEK + +E
Sbjct: 181 GIYVSEKGKIAVEE 194
>At5g56000.1 68418.m06988 heat shock protein 81-4 (HSP81-4) nearly
identical to heat shock protein hsp81.4 [Arabidopsis
thaliana] GI:1906828; contains Pfam profiles PF02518:
ATPase, histidine kinase-, DNA gyrase B-, and HSP90-like
domain protein, PF00183: Hsp90 protein
Length = 699
Score = 61.3 bits (142), Expect = 2e-09
Identities = 28/68 (41%), Positives = 43/68 (63%)
Frame = +1
Query: 742 KLSDLLRYHTSASGDEACSLKSMFPA*KKPKNTSITLLVRTXTXVANSSFVERVKKRGYE 921
K+++LLRYH++ SGDE SLK K+ +N + + V NS F+E++KK+GYE
Sbjct: 429 KIAELLRYHSTKSGDELTSLKDYVTRMKEGQNEIFYITGESKKAVENSPFLEKLKKKGYE 488
Query: 922 XVYMTDPL 945
+YM D +
Sbjct: 489 VLYMVDAI 496
Score = 55.2 bits (127), Expect = 1e-07
Identities = 32/75 (42%), Positives = 43/75 (57%)
Frame = +3
Query: 798 LEEYVSRMKETQKHIYYITGENRDXSC*LIICGKSQEAWV*GCIHD*PIDEXVVQQMKEF 977
L++YV+RMKE Q I+YITGE++ K ++ IDE + Q+KEF
Sbjct: 448 LKDYVTRMKEGQNEIFYITGESKKAVENSPFLEKLKKKGYEVLYMVDAIDEYAIGQLKEF 507
Query: 978 DGKTLVSXXKXGLKL 1022
+GK LVS K GLKL
Sbjct: 508 EGKKLVSATKEGLKL 522
>At5g52640.1 68418.m06535 heat shock protein 81-1 (HSP81-1) / heat
shock protein 83 (HSP83) nearly identical to SP|P27323
Heat shock protein 81-1 (HSP81-1) (Heat shock protein
83) {Arabidopsis thaliana}; contains Pfam profiles
PF02518: ATPase, histidine kinase-, DNA gyrase B-, and
HSP90-like domain protein, PF00183: Hsp90 protein
Length = 705
Score = 61.3 bits (142), Expect = 2e-09
Identities = 29/68 (42%), Positives = 41/68 (60%)
Frame = +1
Query: 742 KLSDLLRYHTSASGDEACSLKSMFPA*KKPKNTSITLLVRTXTXVANSSFVERVKKRGYE 921
K++DLLRYH++ SGDE S K K+ + + + V NS F+ER+KKRGYE
Sbjct: 435 KIADLLRYHSTKSGDEMTSFKDYVTRMKEGQKDIFYITGESKKAVENSPFLERLKKRGYE 494
Query: 922 XVYMTDPL 945
+YM D +
Sbjct: 495 VLYMVDAI 502
Score = 55.6 bits (128), Expect = 8e-08
Identities = 33/83 (39%), Positives = 46/83 (55%)
Frame = +3
Query: 801 EEYVSRMKETQKHIYYITGENRDXSC*LIICGKSQEAWV*GCIHD*PIDEXVVQQMKEFD 980
++YV+RMKE QK I+YITGE++ + ++ IDE V Q+KE+D
Sbjct: 455 KDYVTRMKEGQKDIFYITGESKKAVENSPFLERLKKRGYEVLYMVDAIDEYAVGQLKEYD 514
Query: 981 GKTLVSXXKXGLKLPXMRXKENR 1049
GK LVS K GLKL +E +
Sbjct: 515 GKKLVSATKEGLKLEDETEEEKK 537
>At5g56030.1 68418.m06991 heat shock protein 81-2 (HSP81-2) nearly
identical to SP|P55737 Heat shock protein 81-2 (HSP81-2)
{Arabidopsis thaliana}
Length = 699
Score = 58.0 bits (134), Expect = 2e-08
Identities = 27/68 (39%), Positives = 42/68 (61%)
Frame = +1
Query: 742 KLSDLLRYHTSASGDEACSLKSMFPA*KKPKNTSITLLVRTXTXVANSSFVERVKKRGYE 921
K+++LLRYH++ SGDE SLK K+ +N + + V NS F+E++KK+G E
Sbjct: 429 KIAELLRYHSTKSGDELTSLKDYVTRMKEGQNDIFYITGESKKAVENSPFLEKLKKKGIE 488
Query: 922 XVYMTDPL 945
+YM D +
Sbjct: 489 VLYMVDAI 496
Score = 56.4 bits (130), Expect = 5e-08
Identities = 32/75 (42%), Positives = 44/75 (58%)
Frame = +3
Query: 798 LEEYVSRMKETQKHIYYITGENRDXSC*LIICGKSQEAWV*GCIHD*PIDEXVVQQMKEF 977
L++YV+RMKE Q I+YITGE++ K ++ + IDE + Q+KEF
Sbjct: 448 LKDYVTRMKEGQNDIFYITGESKKAVENSPFLEKLKKKGIEVLYMVDAIDEYAIGQLKEF 507
Query: 978 DGKTLVSXXKXGLKL 1022
+GK LVS K GLKL
Sbjct: 508 EGKKLVSATKEGLKL 522
>At5g56010.1 68418.m06989 heat shock protein, putative strong
similarity to SP|P55737 Heat shock protein 81-2
(HSP81-2) {Arabidopsis thaliana}; contains Pfam profiles
PF02518: ATPase, histidine kinase-, DNA gyrase B-, and
HSP90-like domain protein, PF00183: Hsp90 protein
Length = 699
Score = 58.0 bits (134), Expect = 2e-08
Identities = 27/68 (39%), Positives = 42/68 (61%)
Frame = +1
Query: 742 KLSDLLRYHTSASGDEACSLKSMFPA*KKPKNTSITLLVRTXTXVANSSFVERVKKRGYE 921
K+++LLRYH++ SGDE SLK K+ +N + + V NS F+E++KK+G E
Sbjct: 429 KIAELLRYHSTKSGDELTSLKDYVTRMKEGQNDIFYITGESKKAVENSPFLEKLKKKGIE 488
Query: 922 XVYMTDPL 945
+YM D +
Sbjct: 489 VLYMVDAI 496
Score = 56.4 bits (130), Expect = 5e-08
Identities = 32/75 (42%), Positives = 44/75 (58%)
Frame = +3
Query: 798 LEEYVSRMKETQKHIYYITGENRDXSC*LIICGKSQEAWV*GCIHD*PIDEXVVQQMKEF 977
L++YV+RMKE Q I+YITGE++ K ++ + IDE + Q+KEF
Sbjct: 448 LKDYVTRMKEGQNDIFYITGESKKAVENSPFLEKLKKKGIEVLYMVDAIDEYAIGQLKEF 507
Query: 978 DGKTLVSXXKXGLKL 1022
+GK LVS K GLKL
Sbjct: 508 EGKKLVSATKEGLKL 522
>At4g24190.2 68417.m03473 shepherd protein (SHD) / clavata formation
protein, putative nearly identical to SHEPHERD
[Arabidopsis thaliana] GI:19570872; contains Pfam
profiles PF02518: ATPase, histidine kinase-, DNA gyrase
B-, and HSP90-like domain protein, PF00183: Hsp90
protein
Length = 823
Score = 42.3 bits (95), Expect = 8e-04
Identities = 21/68 (30%), Positives = 36/68 (52%)
Frame = +1
Query: 742 KLSDLLRYHTSASGDEACSLKSMFPA*KKPKNTSITLLVRTXTXVANSSFVERVKKRGYE 921
+L+ LLR+ T+ S + SL KK + + + + S F+ER+ K+GYE
Sbjct: 535 RLAKLLRFETTKSDGKLTSLDQYIKRMKKSQKDIFYITGSSKEQLEKSPFLERLIKKGYE 594
Query: 922 XVYMTDPL 945
++ TDP+
Sbjct: 595 VIFFTDPV 602
>At4g24190.1 68417.m03472 shepherd protein (SHD) / clavata formation
protein, putative nearly identical to SHEPHERD
[Arabidopsis thaliana] GI:19570872; contains Pfam
profiles PF02518: ATPase, histidine kinase-, DNA gyrase
B-, and HSP90-like domain protein, PF00183: Hsp90
protein
Length = 823
Score = 42.3 bits (95), Expect = 8e-04
Identities = 21/68 (30%), Positives = 36/68 (52%)
Frame = +1
Query: 742 KLSDLLRYHTSASGDEACSLKSMFPA*KKPKNTSITLLVRTXTXVANSSFVERVKKRGYE 921
+L+ LLR+ T+ S + SL KK + + + + S F+ER+ K+GYE
Sbjct: 535 RLAKLLRFETTKSDGKLTSLDQYIKRMKKSQKDIFYITGSSKEQLEKSPFLERLIKKGYE 594
Query: 922 XVYMTDPL 945
++ TDP+
Sbjct: 595 VIFFTDPV 602
>At3g07770.1 68416.m00947 heat shock protein-related strong similarity
to heat-shock protein [Secale cereale] GI:556673;
contains Pfam profiles PF02518: ATPase, histidine
kinase-, DNA gyrase B-, and HSP90-like domain protein,
PF00183: Hsp90 protein
Length = 803
Score = 39.5 bits (88), Expect = 0.006
Identities = 27/90 (30%), Positives = 39/90 (43%)
Frame = +3
Query: 798 LEEYVSRMKETQKHIYYITGENRDXSC*LIICGKSQEAWV*GCIHD*PIDEXVVQQMKEF 977
L+EYV MK QK IY+I ++ + K E + PIDE VQ +K +
Sbjct: 543 LDEYVENMKPEQKAIYFIASDSITSAKNAPFLEKMLEKGLEVLYLVEPIDEVAVQSLKAY 602
Query: 978 DGKTLVSXXKXGLKLPXMRXKENRGXQVSL 1067
K V K L L M +N + ++
Sbjct: 603 KEKDFVDISKEDLDLGNMSGDKNEEKEAAV 632
>At1g05190.1 68414.m00523 ribosomal protein L6 family protein
Similar to Mycobacterium RlpF (gb|Z84395). ESTs
gb|T75785,gb|R30580,gb|T04698 come from this gene
Length = 223
Score = 38.7 bits (86), Expect = 0.010
Identities = 21/86 (24%), Positives = 45/86 (52%)
Frame = +2
Query: 116 VANQKVKIPESLTVHVKSRLVTVKGPRGVLKRNFKHLAVDIRMVNPRQLKVEKWFGSKKE 295
+ Q + +P ++T+ ++ + + VKGP G L + V++ L+V+K +++
Sbjct: 46 IGKQPIAVPSNVTIALEGQDLKVKGPLGELALTYPR-EVELTKEESGFLRVKKTVETRRA 104
Query: 296 LAAVRTVCSHVENMIKGVTKGFQYKM 373
+ +NM+ GV+KGF+ K+
Sbjct: 105 NQMHGLFRTLTDNMVVGVSKGFEKKL 130
>At2g04030.2 68415.m00372 heat shock protein, putative strong
similarity to heat shock protein [Arabidopsis thaliana]
GI:1906830; contains Pfam profiles PF02518: ATPase,
histidine kinase-, DNA gyrase B-, and HSP90-like domain
protein, PF00183: Hsp90 protein
Length = 777
Score = 32.3 bits (70), Expect = 0.86
Identities = 20/75 (26%), Positives = 34/75 (45%)
Frame = +3
Query: 798 LEEYVSRMKETQKHIYYITGENRDXSC*LIICGKSQEAWV*GCIHD*PIDEXVVQQMKEF 977
L++Y+ M E QK IYY+ ++ + K + + PIDE +Q ++ +
Sbjct: 517 LDDYIENMGENQKAIYYLATDSLKSAKSAPFLEKLIQKDIEVLYLVEPIDEVAIQNLQTY 576
Query: 978 DGKTLVSXXKXGLKL 1022
K V K L+L
Sbjct: 577 KEKKFVDISKEDLEL 591
>At2g04030.1 68415.m00371 heat shock protein, putative strong
similarity to heat shock protein [Arabidopsis thaliana]
GI:1906830; contains Pfam profiles PF02518: ATPase,
histidine kinase-, DNA gyrase B-, and HSP90-like domain
protein, PF00183: Hsp90 protein
Length = 780
Score = 32.3 bits (70), Expect = 0.86
Identities = 20/75 (26%), Positives = 34/75 (45%)
Frame = +3
Query: 798 LEEYVSRMKETQKHIYYITGENRDXSC*LIICGKSQEAWV*GCIHD*PIDEXVVQQMKEF 977
L++Y+ M E QK IYY+ ++ + K + + PIDE +Q ++ +
Sbjct: 520 LDDYIENMGENQKAIYYLATDSLKSAKSAPFLEKLIQKDIEVLYLVEPIDEVAIQNLQTY 579
Query: 978 DGKTLVSXXKXGLKL 1022
K V K L+L
Sbjct: 580 KEKKFVDISKEDLEL 594
>At3g58060.1 68416.m06472 cation efflux family protein / metal
tolerance protein, putative (MTPc3) member of the cation
diffusion facilitator (CDF) family, or cation efflux
(CE) family, PMID:11500563
Length = 411
Score = 30.7 bits (66), Expect = 2.6
Identities = 16/43 (37%), Positives = 22/43 (51%)
Frame = -1
Query: 442 TDLNNCVSLSGDTVDREMSVHCTHLVLESLGHTFDHVFDMRAY 314
T + N VSL G + E+ T+LV+ G HV +RAY
Sbjct: 302 TVMENAVSLIGQSAPPEVLQKLTYLVMRQGGDNIKHVDTVRAY 344
>At1g76660.1 68414.m08920 expressed protein
Length = 431
Score = 29.9 bits (64), Expect = 4.6
Identities = 20/68 (29%), Positives = 32/68 (47%), Gaps = 2/68 (2%)
Frame = -1
Query: 340 DHVFDMRAYCPNSS*FFFRTEPFLHFQLARINHANVNSQVFEVSF*NSARTF--HCHQTR 167
D F+ AY P+ R E L Q + + A+++SQV + S+ ++ H + R
Sbjct: 323 DGSFNTSAYSPSDGQKLLRREANLLSQTSPKSEADLDSQVVDFQSPKSSNSYKDHKQRNR 382
Query: 166 FHMDGETL 143
H D E L
Sbjct: 383 IHADEEAL 390
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 25,101,086
Number of Sequences: 28952
Number of extensions: 467169
Number of successful extensions: 1081
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 1036
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1072
length of database: 12,070,560
effective HSP length: 84
effective length of database: 9,638,592
effective search space used: 4028931456
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -