Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030725E6_F05_e518_11.seq
(1568 letters)
Database: bee
438 sequences; 146,343 total letters
Searching......................................................done
Score E
Sequences producing significant alignments: (bits) Value
AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1al... 429 e-122
X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alp... 413 e-117
AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor 1-a... 408 e-116
EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor 1-a... 354 1e-99
AF069739-1|AAC63272.2| 690|Apis mellifera translation initiatio... 38 3e-04
EF013227-1|ABK54581.1| 119|Apis mellifera elongation factor 1-a... 35 0.002
AF080430-1|AAC28863.2| 208|Apis mellifera ribosomal protein S8 ... 25 1.8
AB161181-1|BAD08343.1| 933|Apis mellifera metabotropic glutamat... 23 5.4
AB167961-1|BAD51404.1| 554|Apis mellifera E74 protein. 23 9.5
>AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1alpha
F2 protein.
Length = 461
Score = 429 bits (1057), Expect = e-122
Identities = 217/287 (75%), Positives = 225/287 (78%), Gaps = 1/287 (0%)
Frame = +1
Query: 97 MGKGSFKYAWVLDKLKAERERGITIDIALWKFETGKYYVTIIDAPGHRDFIKNMITGTSQ 276
MGKGSFKYAWVLDKLKAERERGITIDIALWKFET KYYVTIIDAPGHRDFIKNMITGTSQ
Sbjct: 49 MGKGSFKYAWVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQ 108
Query: 277 ADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSESRFE 456
ADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSE+RFE
Sbjct: 109 ADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFE 168
Query: 457 EIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKGWLVERKEGKAEGKCL 636
EIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLE S+KMPWFKGW VERKEGK EGKCL
Sbjct: 169 EIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEVSSKMPWFKGWTVERKEGKVEGKCL 228
Query: 637 IEALDAILPPARPTDKALRLPLQGRIQNRRYWNSTPWVELKL-VS*AWYCCRFXPC*HXT 813
IEALDAILPP RPTDKALRLPLQ + + P ++ V F P T
Sbjct: 229 IEALDAILPPTRPTDKALRLPLQD-VYKIGGIGTVPVGRVETGVLKPGMVVTFAPAGLTT 287
Query: 814 EVKSXEXXHEXSTRSXXR*QCWFXXSKTFX*GIAPGYVXGDSKTXHP 954
EVKS E HE + F + GYV GDSK P
Sbjct: 288 EVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPP 334
Score = 75.4 bits (177), Expect = 1e-15
Identities = 52/149 (34%), Positives = 64/149 (42%), Gaps = 2/149 (1%)
Frame = +2
Query: 593 GWLSVKKE-RLKVNALLRPSTPSFRLLAPLTRLSVFPCKDVYKIGGIGTVPRG*S*NWYP 769
GW +KE +++ L+ P + P +DVYKIGGIGTVP G
Sbjct: 213 GWTVERKEGKVEGKCLIEALDAILPPTRPTDKALRLPLQDVYKIGGIGTVPVGRVETGVL 272
Query: 770 KPGTVVVXAPANXLLKSSPXKXPTRXLQEAXPGDNVGXNXQKRXCKELPRG-TXQEIPKX 946
KPG VV APA + + LQEA PGDNVG N + KEL RG +
Sbjct: 273 KPGMVVTFAPAGLTTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNN 332
Query: 947 PTXGSCRXXXXXXXAXXPGQX*XGXHLVL 1033
P G+ PGQ G VL
Sbjct: 333 PPKGAADFTAQVIVLNHPGQISNGYTPVL 361
>X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alpha
protein.
Length = 461
Score = 413 bits (1018), Expect = e-117
Identities = 210/288 (72%), Positives = 223/288 (77%), Gaps = 2/288 (0%)
Frame = +1
Query: 97 MGKGSFKYAWVLDKLKAERERGITIDIALWKFETGKYYVTIIDAPGHRDFIKNMITGTSQ 276
MGKGSFKYAWVLDKLKAERERGITIDIALWKFET KYYVTIIDAPGHRDFIKNMITGTSQ
Sbjct: 49 MGKGSFKYAWVLDKLKAERERGITIDIALWKFETAKYYVTIIDAPGHRDFIKNMITGTSQ 108
Query: 277 ADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSESRFE 456
ADCAVLIVAAG GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMD T+PPYSE+RFE
Sbjct: 109 ADCAVLIVAAGIGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDMTDPPYSEARFE 168
Query: 457 EIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKGWLVERKEGKAEGKCL 636
EIKKEVSSYIKKIGYN A+VAFVPISGWHGDNMLEPS K PW+KGW VERK+G A+GK L
Sbjct: 169 EIKKEVSSYIKKIGYNTASVAFVPISGWHGDNMLEPSPKTPWYKGWKVERKDGNADGKTL 228
Query: 637 IEALDAILPPARPTDKALRLPLQGRIQNRRYWNSTP--WVELKLVS*AWYCCRFXPC*HX 810
IEALDAILPP+RPTDKALRLPLQ + + P VE ++ F P
Sbjct: 229 IEALDAILPPSRPTDKALRLPLQD-VYKIGGIGTVPVGRVETGILK-PGMLVTFAPAALT 286
Query: 811 TEVKSXEXXHEXSTRSXXR*QCWFXXSKTFX*GIAPGYVXGDSKTXHP 954
TEVKS E HE T + F + GYV GDSK P
Sbjct: 287 TEVKSVEMHHEALTEALPGDNVGFNVKNISVKELRRGYVAGDSKNQPP 334
Score = 70.9 bits (166), Expect = 3e-14
Identities = 52/137 (37%), Positives = 64/137 (46%), Gaps = 1/137 (0%)
Frame = +2
Query: 626 VNALLRPSTPSFRLLAPLTRLSVFPCKDVYKIGGIGTVPRG*S*NWYPKPGTVVVXAPAN 805
++A+L PS P+ + L RL P +DVYKIGGIGTVP G KPG +V APA
Sbjct: 232 LDAILPPSRPTDKAL----RL---PLQDVYKIGGIGTVPVGRVETGILKPGMLVTFAPAA 284
Query: 806 XLLKSSPXKXPTRXLQEAXPGDNVGXNXQKRXCKELPRG-TXQEIPKXPTXGSCRXXXXX 982
+ + L EA PGDNVG N + KEL RG + P G+
Sbjct: 285 LTTEVKSVEMHHEALTEALPGDNVGFNVKNISVKELRRGYVAGDSKNQPPRGAADFTAQV 344
Query: 983 XXAXXPGQX*XGXHLVL 1033
PGQ G VL
Sbjct: 345 IVLNHPGQISNGYTPVL 361
>AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor
1-alpha protein.
Length = 274
Score = 408 bits (1005), Expect = e-116
Identities = 207/274 (75%), Positives = 215/274 (78%), Gaps = 1/274 (0%)
Frame = +1
Query: 124 WVLDKLKAERERGITIDIALWKFETGKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 303
WVLDKLKAERERGITIDIALWKFET KYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA
Sbjct: 1 WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 60
Query: 304 AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSESRFEEIKKEVSSY 483
AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSE+RFEEIKKEVSSY
Sbjct: 61 AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSY 120
Query: 484 IKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKGWLVERKEGKAEGKCLIEALDAILP 663
IKKIGYNPAAVAFVPISGWHGDNMLE S+KMPWFKGW VERKEGK EGKCLIEALDAILP
Sbjct: 121 IKKIGYNPAAVAFVPISGWHGDNMLEVSSKMPWFKGWTVERKEGKVEGKCLIEALDAILP 180
Query: 664 PARPTDKALRLPLQGRIQNRRYWNSTPWVELKL-VS*AWYCCRFXPC*HXTEVKSXEXXH 840
P RPTDKALRLPLQ + + P ++ V F P TEVKS E H
Sbjct: 181 PTRPTDKALRLPLQD-VYKIGGIGTVPVGRVETGVLKPGMVVTFAPAGLTTEVKSVEMHH 239
Query: 841 EXSTRSXXR*QCWFXXSKTFX*GIAPGYVXGDSK 942
E + F + GYV GDSK
Sbjct: 240 EALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSK 273
Score = 72.5 bits (170), Expect = 9e-15
Identities = 44/111 (39%), Positives = 54/111 (48%), Gaps = 1/111 (0%)
Frame = +2
Query: 593 GWLSVKKE-RLKVNALLRPSTPSFRLLAPLTRLSVFPCKDVYKIGGIGTVPRG*S*NWYP 769
GW +KE +++ L+ P + P +DVYKIGGIGTVP G
Sbjct: 156 GWTVERKEGKVEGKCLIEALDAILPPTRPTDKALRLPLQDVYKIGGIGTVPVGRVETGVL 215
Query: 770 KPGTVVVXAPANXLLKSSPXKXPTRXLQEAXPGDNVGXNXQKRXCKELPRG 922
KPG VV APA + + LQEA PGDNVG N + KEL RG
Sbjct: 216 KPGMVVTFAPAGLTTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRG 266
>EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor
1-alpha protein.
Length = 172
Score = 354 bits (870), Expect = 1e-99
Identities = 165/172 (95%), Positives = 167/172 (97%)
Frame = +1
Query: 172 DIALWKFETGKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 351
DIALWKFET KYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT
Sbjct: 1 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 60
Query: 352 REHALLAFTLGVKQLIVGVNKMDSTEPPYSESRFEEIKKEVSSYIKKIGYNPAAVAFVPI 531
REHALLAFTLGVKQLIVGVNKMDSTEPPYSE+RFEEIKKEVSSYIKKIGYNPAAVAFVPI
Sbjct: 61 REHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFVPI 120
Query: 532 SGWHGDNMLEPSTKMPWFKGWLVERKEGKAEGKCLIEALDAILPPARPTDKA 687
SGWHGDNMLE S+KMPWFKGW VERKEGK EGKCLIEALDAILPP RPTDKA
Sbjct: 121 SGWHGDNMLEVSSKMPWFKGWTVERKEGKVEGKCLIEALDAILPPTRPTDKA 172
>AF069739-1|AAC63272.2| 690|Apis mellifera translation initiation
factor 2 protein.
Length = 690
Score = 37.5 bits (83), Expect = 3e-04
Identities = 36/117 (30%), Positives = 52/117 (44%)
Frame = +1
Query: 211 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVK 390
VT +D PGH FI G D VL+VAA G E QT + +A V
Sbjct: 195 VTFLDTPGHAAFISMRHRGAHITDIVVLVVAADDGVKE-------QTLQSIEMAKDAKV- 246
Query: 391 QLIVGVNKMDSTEPPYSESRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLE 561
+IV +NK+D + ++E K + I+++G + V IS G N+ E
Sbjct: 247 PIIVAINKIDKPNIDIIKVQYELAKHGI--VIEELG---GEIQCVKISALKGINLRE 298
>EF013227-1|ABK54581.1| 119|Apis mellifera elongation factor 1-alpha
protein.
Length = 119
Score = 35.1 bits (77), Expect = 0.002
Identities = 23/63 (36%), Positives = 26/63 (41%), Gaps = 1/63 (1%)
Frame = +2
Query: 848 LQEAXPGDNVGXNXQKRXCKELPRG-TXQEIPKXPTXGSCRXXXXXXXAXXPGQX*XGXH 1024
L EA PGDNVG N + KEL RG + P G+ PGQ G
Sbjct: 10 LTEALPGDNVGFNVKNISVKELRRGYVAGDSKNQPPRGAADFTAQVIVLNHPGQISNGYT 69
Query: 1025 LVL 1033
VL
Sbjct: 70 PVL 72
>AF080430-1|AAC28863.2| 208|Apis mellifera ribosomal protein S8
protein.
Length = 208
Score = 25.0 bits (52), Expect = 1.8
Identities = 14/36 (38%), Positives = 19/36 (52%), Gaps = 1/36 (2%)
Frame = +2
Query: 512 PSLSYPFLAGTEITC-WSRPPKCHGSRDGWLSVKKE 616
P+L F G + C SRP +C G DG++ KE
Sbjct: 160 PALEEQFATGRVLACISSRPGQC-GREDGYILEGKE 194
>AB161181-1|BAD08343.1| 933|Apis mellifera metabotropic glutamate
receptor protein.
Length = 933
Score = 23.4 bits (48), Expect = 5.4
Identities = 12/44 (27%), Positives = 20/44 (45%)
Frame = +2
Query: 596 WLSVKKERLKVNALLRPSTPSFRLLAPLTRLSVFPCKDVYKIGG 727
W++ E+ K RP LL L RLS++ +++ G
Sbjct: 3 WINRDGEKWKGKMSQRPLARMLALLVSLHRLSIYSGGSDWRVAG 46
>AB167961-1|BAD51404.1| 554|Apis mellifera E74 protein.
Length = 554
Score = 22.6 bits (46), Expect = 9.5
Identities = 8/17 (47%), Positives = 12/17 (70%)
Frame = -3
Query: 516 DGSRIVTNFLDVGRDFL 466
DG R+V F+DV +D +
Sbjct: 531 DGQRLVYQFVDVPKDII 547
Database: bee
Posted date: Oct 23, 2007 1:17 PM
Number of letters in database: 146,343
Number of sequences in database: 438
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 358,429
Number of Sequences: 438
Number of extensions: 7376
Number of successful extensions: 39
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 20
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 24
length of database: 146,343
effective HSP length: 61
effective length of database: 119,625
effective search space used: 55147125
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -