Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030725E6_F04_e510_12.seq
(1507 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g22390.1 68418.m02612 expressed protein 35 0.12
At5g26160.1 68418.m03111 expressed protein 31 1.5
At2g40740.1 68415.m05025 WRKY family transcription factor contai... 31 2.6
At1g79860.1 68414.m09329 expressed protein contains Pfam profile... 31 2.6
At2g21470.2 68415.m02555 SUMO activating enzyme 2 (SAE2) nearly ... 30 3.4
At2g21470.1 68415.m02554 SUMO activating enzyme 2 (SAE2) nearly ... 30 3.4
At1g09460.1 68414.m01058 glucan endo-1,3-beta-glucosidase-relate... 30 3.4
At1g19870.1 68414.m02492 calmodulin-binding family protein conta... 29 6.0
At1g01730.1 68414.m00092 expressed protein 29 8.0
>At5g22390.1 68418.m02612 expressed protein
Length = 202
Score = 35.1 bits (77), Expect = 0.12
Identities = 21/60 (35%), Positives = 31/60 (51%)
Frame = +3
Query: 249 PATEIIGELPARDKSPSEVETTQEKIISLELTTSPNNEATRVDIEDAGISLGEGEAEVGE 428
P TEI GEL R+ S S E T + SL+L T + +ED ++ G+G+ + E
Sbjct: 51 PFTEIFGELHFRESSHSSYEKTSAENSSLQLCTEGLGSESYYGLEDEKVN-GDGDGDDDE 109
>At5g26160.1 68418.m03111 expressed protein
Length = 1058
Score = 31.5 bits (68), Expect = 1.5
Identities = 19/58 (32%), Positives = 30/58 (51%)
Frame = +3
Query: 285 DKSPSEVETTQEKIISLELTTSPNNEATRVDIEDAGISLGEGEAEVGEHSLQPDIVQV 458
D S VET Q++ LE + PN E++R++I D L + + V E + D + V
Sbjct: 315 DDSGKGVETFQQERSGLEESNDPNTESSRIEIIDVHEILKDEDESVFEETYFIDQLSV 372
>At2g40740.1 68415.m05025 WRKY family transcription factor contains
Pfam profile: PF03106 WRKY DNA -binding domain
Length = 292
Score = 30.7 bits (66), Expect = 2.6
Identities = 15/37 (40%), Positives = 26/37 (70%), Gaps = 1/37 (2%)
Frame = -1
Query: 427 SPTSASPSPKLIPASSISTRVASLFGL-VVNSNDIIF 320
+PT++S +P IP SS++T + +GL VV+ D++F
Sbjct: 237 APTASSATPSTIPISSVTTGHSVDYGLAVVDMADVMF 273
>At1g79860.1 68414.m09329 expressed protein contains Pfam profile
PF03759: Domain of unknown function (DUF315)
Length = 515
Score = 30.7 bits (66), Expect = 2.6
Identities = 16/59 (27%), Positives = 30/59 (50%)
Frame = +3
Query: 291 SPSEVETTQEKIISLELTTSPNNEATRVDIEDAGISLGEGEAEVGEHSLQPDIVQVVNP 467
S E ET + ++ + + + NN ATR + + + G EA G H +P + +++P
Sbjct: 5 SEQEQETYRSRLFNFKWRNNDNNSATRHN-KSLSVETGLDEAATGSHDAEP--LTIIHP 60
>At2g21470.2 68415.m02555 SUMO activating enzyme 2 (SAE2) nearly
identical to SUMO activating enzyme 2 [Arabidopsis
thaliana] GI:22652854; contains Pfam profiles PF00899:
ThiF family, PF02134: Repeat in ubiquitin-activating
(UBA) protein
Length = 700
Score = 30.3 bits (65), Expect = 3.4
Identities = 17/68 (25%), Positives = 31/68 (45%), Gaps = 2/68 (2%)
Frame = -1
Query: 466 GFTT*TISG*RECSPTSASPSPKLIPASSISTRVASLFGLVVNSNDIIFSCVV--STSDG 293
G T I G EC P+PK P +I++ +V + D++F+ + D
Sbjct: 141 GQVTVHIKGKTECYECQTKPAPKTYPVCTITSTPTKFVHCIVWAKDLLFAKLFGDKNQDN 200
Query: 292 DLSLAGSS 269
DL++ ++
Sbjct: 201 DLNVRSNN 208
>At2g21470.1 68415.m02554 SUMO activating enzyme 2 (SAE2) nearly
identical to SUMO activating enzyme 2 [Arabidopsis
thaliana] GI:22652854; contains Pfam profiles PF00899:
ThiF family, PF02134: Repeat in ubiquitin-activating
(UBA) protein
Length = 625
Score = 30.3 bits (65), Expect = 3.4
Identities = 17/68 (25%), Positives = 31/68 (45%), Gaps = 2/68 (2%)
Frame = -1
Query: 466 GFTT*TISG*RECSPTSASPSPKLIPASSISTRVASLFGLVVNSNDIIFSCVV--STSDG 293
G T I G EC P+PK P +I++ +V + D++F+ + D
Sbjct: 141 GQVTVHIKGKTECYECQTKPAPKTYPVCTITSTPTKFVHCIVWAKDLLFAKLFGDKNQDN 200
Query: 292 DLSLAGSS 269
DL++ ++
Sbjct: 201 DLNVRSNN 208
>At1g09460.1 68414.m01058 glucan endo-1,3-beta-glucosidase-related
similar to glucan endo-1,3-beta-glucosidase precursor
SP:P52409 from [Triticum aestivum]
Length = 330
Score = 30.3 bits (65), Expect = 3.4
Identities = 12/32 (37%), Positives = 18/32 (56%)
Frame = +1
Query: 511 GPRLNSPLTQKNHNKFHLQNFIQKTQSPVSCD 606
G SP++ ++H F ++ QK SP SCD
Sbjct: 168 GGNCYSPISLQSHASFAFNSYYQKNPSPQSCD 199
>At1g19870.1 68414.m02492 calmodulin-binding family protein contains
Pfam profile: PF00612 IQ calmodulin-binding motif
Length = 794
Score = 29.5 bits (63), Expect = 6.0
Identities = 13/56 (23%), Positives = 30/56 (53%)
Frame = +3
Query: 195 SLEQEAIESQKVIKSMVGPATEIIGELPARDKSPSEVETTQEKIISLELTTSPNNE 362
+L + A+ + +S PA++ + AR + ++Q++ +S ++T+SP E
Sbjct: 629 ALSEGALTPMTITESQATPASQASSSVKARKGKSEKSGSSQKRKVSKKITSSPKQE 684
>At1g01730.1 68414.m00092 expressed protein
Length = 224
Score = 29.1 bits (62), Expect = 8.0
Identities = 21/71 (29%), Positives = 33/71 (46%)
Frame = +3
Query: 213 IESQKVIKSMVGPATEIIGELPARDKSPSEVETTQEKIISLELTTSPNNEATRVDIEDAG 392
IE Q+ +K M+ E+IGE P R+K+ + K + TT+ R +
Sbjct: 110 IEIQEKMKLMLQLYEEMIGESPKREKTAKSDSLSNGKATT---TTTTTTSVLRSSETEKH 166
Query: 393 ISLGEGEAEVG 425
S G+G+ VG
Sbjct: 167 SSDGDGDKVVG 177
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 21,274,387
Number of Sequences: 28952
Number of extensions: 383456
Number of successful extensions: 877
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 850
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 877
length of database: 12,070,560
effective HSP length: 84
effective length of database: 9,638,592
effective search space used: 4019292864
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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