Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030725E6_E11_e565_09.seq
(1558 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 33 0.081
SPBC16G5.09 |||serine carboxypeptidase |Schizosaccharomyces pomb... 29 2.3
SPCC553.01c ||SPCC736.01c|meiotic chromosome segregation protein... 28 4.0
SPAC1486.05 |nup189||nucleoporin Nup189|Schizosaccharomyces pomb... 28 4.0
SPBC1105.04c |cbp1|abp1|CENP-B homolog|Schizosaccharomyces pombe... 27 5.3
SPBC725.06c |ppk31|mug25|serine/threonine protein kinase Ppk31 |... 27 9.3
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with
EF hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 33.5 bits (73), Expect = 0.081
Identities = 27/105 (25%), Positives = 40/105 (38%), Gaps = 3/105 (2%)
Frame = +1
Query: 130 MSPMLPQGVPPQVADPFINLPIDFVSNGKPTGVYQWSGAPGFQQVAQMPVDPFSYGSQPI 309
M PM+PQ Q P + P+ G + Q +G Q P+ P G QP+
Sbjct: 680 MQPMMPQRTGMQPQMPGMQQPMAPQRTGMQPMMPQRTGMQPQMPGMQQPMAPQRTGMQPM 739
Query: 310 LPSRPNYNVPSYQSKPKPQQTN---TKITNLKGQYVFNGKPADSV 435
P R PQ+T + T + GQ+ F P ++
Sbjct: 740 APQRTGMQPQMTGGPMLPQRTGGMAPQPTGMPGQWGFINTPLSNL 784
Score = 32.3 bits (70), Expect = 0.19
Identities = 23/81 (28%), Positives = 33/81 (40%), Gaps = 2/81 (2%)
Frame = +1
Query: 136 PMLPQGVPPQVADPFINLPIDFVSNGKPTGVYQWSGAPGFQQVAQMPVDPFSYGSQPILP 315
PM+PQ Q P + P+ G + Q +G Q P+ P G QP++P
Sbjct: 511 PMVPQRTGMQPMMPGLQQPMAPQRTGMQPMMPQRTGMQPQMTGFQQPMAPQRTGMQPMMP 570
Query: 316 SRPNY--NVPSYQSKPKPQQT 372
R +P Q PQ+T
Sbjct: 571 QRTGMQPQMPGMQQPMAPQRT 591
Score = 31.1 bits (67), Expect = 0.43
Identities = 27/93 (29%), Positives = 37/93 (39%), Gaps = 12/93 (12%)
Frame = +1
Query: 130 MSPMLPQGVPPQVADPFINLPIDFVSNGKPTGVYQWSGA----PGFQQ------VAQMPV 279
M PM+PQ Q P + P+ G + Q +G PG QQ P+
Sbjct: 614 MQPMMPQRTGMQPQMPGMQQPMAPQRTGMQPMMPQRTGMQPQMPGMQQPMAPQRTGMQPM 673
Query: 280 DPFSYGSQPILPSRPNY--NVPSYQSKPKPQQT 372
P G QP++P R +P Q PQ+T
Sbjct: 674 APQRTGMQPMMPQRTGMQPQMPGMQQPMAPQRT 706
Score = 29.9 bits (64), Expect = 0.99
Identities = 24/93 (25%), Positives = 37/93 (39%), Gaps = 2/93 (2%)
Frame = +1
Query: 130 MSPMLPQ--GVPPQVADPFINLPIDFVSNGKPTGVYQWSGAPGFQQVAQMPVDPFSYGSQ 303
M PM+PQ G+ PQ+ P+ G + Q +G Q P+ P G Q
Sbjct: 537 MQPMMPQRTGMQPQMTG--FQQPMAPQRTGMQPMMPQRTGMQPQMPGMQQPMAPQRTGMQ 594
Query: 304 PILPSRPNYNVPSYQSKPKPQQTNTKITNLKGQ 402
P++P R P + Q + T ++ Q
Sbjct: 595 PMMPQRTGMQQPMAPQRTGMQPMMPQRTGMQPQ 627
>SPBC16G5.09 |||serine carboxypeptidase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 510
Score = 28.7 bits (61), Expect = 2.3
Identities = 13/37 (35%), Positives = 19/37 (51%)
Frame = +1
Query: 196 DFVSNGKPTGVYQWSGAPGFQQVAQMPVDPFSYGSQP 306
+++S K T +W+GA GFQ + P Y QP
Sbjct: 371 NYLSGEKLTRSLEWNGAVGFQNQSAQPFYLPGYSDQP 407
>SPCC553.01c ||SPCC736.01c|meiotic chromosome segregation
protein|Schizosaccharomyces pombe|chr 3|||Manual
Length = 715
Score = 27.9 bits (59), Expect = 4.0
Identities = 31/112 (27%), Positives = 47/112 (41%), Gaps = 1/112 (0%)
Frame = +1
Query: 100 ISQPPTI-GPPMSPMLPQGVPPQVADPFINLPIDFVSNGKPTGVYQWSGAPGFQQVAQMP 276
IS PT+ +SP Q PP++ +P F GK + + P FQ+ +P
Sbjct: 609 ISSSPTVRAVNLSPSKNQK-PPKI---LTKIPDIFSKAGKKSPIL-----PTFQESCSLP 659
Query: 277 VDPFSYGSQPILPSRPNYNVPSYQSKPKPQQTNTKITNLKGQYVFNGKPADS 432
++PFS + LP + S SK K + K+ K G+ DS
Sbjct: 660 IEPFS-SNITDLPFVNTVTLASTSSKIK--RAKLKMLKFKHAMTTQGRDLDS 708
>SPAC1486.05 |nup189||nucleoporin Nup189|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1778
Score = 27.9 bits (59), Expect = 4.0
Identities = 17/59 (28%), Positives = 26/59 (44%)
Frame = +1
Query: 220 TGVYQWSGAPGFQQVAQMPVDPFSYGSQPILPSRPNYNVPSYQSKPKPQQTNTKITNLK 396
TG++ S A QQ Q +D YG+ P+ S + P+ +P +K T K
Sbjct: 616 TGLFGGSQAGQQQQPLQASIDQNPYGNNPLFSSTTSQVAPTSIQEPIASPLTSKPTPKK 674
>SPBC1105.04c |cbp1|abp1|CENP-B homolog|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 522
Score = 27.5 bits (58), Expect = 5.3
Identities = 14/45 (31%), Positives = 25/45 (55%), Gaps = 3/45 (6%)
Frame = +1
Query: 328 YNVPSYQSKPKPQQTNTKITNLKGQYV---FNGKPADSVYVLRNT 453
+ +P YQ +P P +N + + +++ N +P +SV VL NT
Sbjct: 113 HKIPEYQDQPVPNFSNGWLEGFRKRHILHAINEQPTESV-VLNNT 156
>SPBC725.06c |ppk31|mug25|serine/threonine protein kinase Ppk31
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1032
Score = 26.6 bits (56), Expect = 9.3
Identities = 14/52 (26%), Positives = 27/52 (51%), Gaps = 1/52 (1%)
Frame = -2
Query: 861 VGIGTKVLANHKVGFRAINYFPL-PQQRQAVALQELTLIKYYYRLLPVMCVI 709
+GIG +L +H +G NY + P + +L E L ++Y+ + C++
Sbjct: 151 LGIGAFILHDHLLGIIKYNYIHVPPPSGKLCSLCEDNLPEWYFEVHSDFCLV 202
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 4,525,448
Number of Sequences: 5004
Number of extensions: 88926
Number of successful extensions: 246
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 220
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 242
length of database: 2,362,478
effective HSP length: 76
effective length of database: 1,982,174
effective search space used: 876120908
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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