BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030725E6_E05_e517_09.seq
(1617 letters)
Database: nematostella
59,808 sequences; 16,821,457 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SB_35225| Best HMM Match : Ribosomal_L18p (HMM E-Value=4e-30) 114 2e-25
SB_12441| Best HMM Match : Ribosomal_L18p (HMM E-Value=0) 106 6e-23
>SB_35225| Best HMM Match : Ribosomal_L18p (HMM E-Value=4e-30)
Length = 113
Score = 114 bits (275), Expect = 2e-25
Identities = 50/88 (56%), Positives = 60/88 (68%)
Frame = +1
Query: 301 VAYSRIEGDHIVCAAYSHELPRYGIKVGLTNYAAAYCTGXXXXXXXXXXXXXDSLYTGAT 480
+AY+++EGD I+CAAY+HELPRYG+KVGLTNYAAAYCTG +YTG
Sbjct: 1 IAYAKLEGDVIICAAYAHELPRYGVKVGLTNYAAAYCTGLLLARRLLTKLNLHEIYTGTE 60
Query: 481 EVTGDEYNVEPVDNGPGAFMMLLGCRLS 564
EV GDEYNVE +D PGAF L L+
Sbjct: 61 EVNGDEYNVESIDGSPGAFRCFLDVGLA 88
Score = 39.9 bits (89), Expect = 0.006
Identities = 16/19 (84%), Positives = 18/19 (94%)
Frame = +3
Query: 582 ARVFGAMKGAVDGGLXVPH 638
ARVFGA+KGAVDGGL +PH
Sbjct: 94 ARVFGALKGAVDGGLEIPH 112
>SB_12441| Best HMM Match : Ribosomal_L18p (HMM E-Value=0)
Length = 328
Score = 106 bits (254), Expect = 6e-23
Identities = 66/167 (39%), Positives = 86/167 (51%), Gaps = 37/167 (22%)
Frame = +1
Query: 175 RRREGKTDYYARKR------------------------LVVQDKNKYNTP------KYRL 264
RR +GKTDYYARKR ++ Q++NK P KYR
Sbjct: 17 RRSQGKTDYYARKRLITQDKNKYNTPKYRFVVRITNKDIICQERNKVGGPIFGSTQKYRR 76
Query: 265 IVRLS-NK------DVTCQVAYSRIEGDHIVCAAYSHELPRYGIKVGLTNYAAAYCTGXX 423
R NK ++AY++++GD ++ +AY+HELP +G+KVGLTNYAAAYCTG
Sbjct: 77 NSRGKYNKRNIFILQTYARIAYAKLDGDRVLASAYAHELPNFGVKVGLTNYAAAYCTGLL 136
Query: 424 XXXXXXXXXXXDSLYTGATEVTGDEYNVEPVDNGPGAFMMLLGCRLS 564
+YTG +V GDEYNVE VD PGAF L L+
Sbjct: 137 LARRLLTMLNLHEIYTGTDDVNGDEYNVESVDGSPGAFRCFLDVGLA 183
Score = 59.3 bits (137), Expect = 9e-09
Identities = 25/31 (80%), Positives = 29/31 (93%)
Frame = +3
Query: 582 ARVFGAMKGAVDGGLXVPHSIKRFXGYDAES 674
ARVFGA+KGAVDGGL +PHS+KRF GYD+ES
Sbjct: 189 ARVFGALKGAVDGGLEIPHSMKRFPGYDSES 219
Score = 32.7 bits (71), Expect = 0.85
Identities = 23/66 (34%), Positives = 29/66 (43%)
Frame = +2
Query: 542 CYLDVGLARTTTGRSRIWCHEGRR*WRFKXSTFYQEVXWI*C*I*XNLMQXXHRXHIFGL 721
C+LDVGLART+TG +G + + + HR HIFG
Sbjct: 176 CFLDVGLARTSTGARVFGALKGAVDGGLEIPHSMKRFPGYDSES-KDFSAEVHRNHIFGK 234
Query: 722 HVAXYM 739
HVA YM
Sbjct: 235 HVAEYM 240
Database: nematostella
Posted date: Oct 22, 2007 1:22 PM
Number of letters in database: 16,821,457
Number of sequences in database: 59,808
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 30,511,448
Number of Sequences: 59808
Number of extensions: 549656
Number of successful extensions: 1154
Number of sequences better than 10.0: 2
Number of HSP's better than 10.0 without gapping: 1021
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1154
length of database: 16,821,457
effective HSP length: 86
effective length of database: 11,677,969
effective search space used: 5278441988
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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