BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030725E6_E04_e509_10.seq
(1534 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC36B7.04 |||tRNA dihydrouridine synthase Dus1 |Schizosaccharo... 33 0.14
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 28 3.9
SPAC3G9.13c |msw1||mitochondrial tryptophan-tRNA ligase Msw1 |Sc... 28 3.9
SPAC2H10.01 |||transcription factor, zf-fungal binuclear cluster... 28 3.9
SPAC1F7.05 |cdc22||ribonucleoside reductase large subunit Cdc22|... 27 6.9
SPBC21.05c |ral2||Ras guanyl-nucleotide exchange factor Ral2 |Sc... 27 9.1
>SPBC36B7.04 |||tRNA dihydrouridine synthase Dus1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 399
Score = 32.7 bits (71), Expect = 0.14
Identities = 23/88 (26%), Positives = 41/88 (46%), Gaps = 3/88 (3%)
Frame = +2
Query: 335 NEEDWTLLSKKAEAAGADALELNLSCPHGMGESG-MGLACGQDPVLVKGISQWVRKAIKI 511
N+ + L + K A DA+++NL CP G+ + G G ++ L++ I + + I
Sbjct: 84 NDPEIMLKAAKIAAPYCDAVDVNLGCPQGIAKKGKYGSFLQENWNLIESIITKLHTELSI 143
Query: 512 PFFVKLT--PNITDIVSIAVAAYEGGAS 589
P K+ P+ + A + GAS
Sbjct: 144 PVTAKIRIFPDPQKTLDYAKMILKAGAS 171
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 27.9 bits (59), Expect = 3.9
Identities = 23/83 (27%), Positives = 31/83 (37%)
Frame = -2
Query: 807 SADSAESIPPIPRIGKPGSLLPIAETARSPIGRVALPDSPPYVVCFSRPTAGHGVPSALI 628
++ SA IPP +P +P P P PP S P P A +
Sbjct: 225 TSTSAPPIPPSIPSSRPPERVPSLSAPAPP------PIPPPSNGTVSSPPNSPPRPIAPV 278
Query: 627 DINPDTVFIADTPLAPPSYAATA 559
+NP + PL PPS +A
Sbjct: 279 SMNPAINSTSKPPLPPPSSRVSA 301
>SPAC3G9.13c |msw1||mitochondrial tryptophan-tRNA ligase Msw1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 361
Score = 27.9 bits (59), Expect = 3.9
Identities = 26/93 (27%), Positives = 43/93 (46%), Gaps = 10/93 (10%)
Frame = +2
Query: 170 VRGVTSGENYGPGQGSFLNI-ELISEKCEAYWCQSVTELKK-DFPNKVIIASIMC----- 328
++G+T G++ PG + +NI IS+ + Q+ +F KV A I C
Sbjct: 258 IKGITYGDSNRPGINNLINIFAAISDSTPSDIAQANASCSNAEFKEKVSSAIIRCLQPIS 317
Query: 329 -SYNE--EDWTLLSKKAEAAGADALELNLSCPH 418
S+NE ++ LL A+ +A+ SC H
Sbjct: 318 TSFNEWRQNRELLRDIAKKGAEEAVAEASSCMH 350
>SPAC2H10.01 |||transcription factor, zf-fungal binuclear cluster
type|Schizosaccharomyces pombe|chr 1|||Manual
Length = 480
Score = 27.9 bits (59), Expect = 3.9
Identities = 12/33 (36%), Positives = 17/33 (51%)
Frame = -2
Query: 579 PSYAATAMLTMSVIFGVSFTKNGILIALRTHCD 481
PSYA+T L+ + +G LI+ HCD
Sbjct: 76 PSYASTPSLSPMTSSHFPYASDGTLISATNHCD 108
>SPAC1F7.05 |cdc22||ribonucleoside reductase large subunit
Cdc22|Schizosaccharomyces pombe|chr 1|||Manual
Length = 811
Score = 27.1 bits (57), Expect = 6.9
Identities = 16/50 (32%), Positives = 23/50 (46%), Gaps = 2/50 (4%)
Frame = -1
Query: 877 NCEVLILYSTAYLYDRCTAHYK-LQCGFCRINPTDSQDW-ETRESVTDSG 734
+CE+ + T Y+ A LQ +NPTD DW E +E + G
Sbjct: 542 SCEIAQVEGTYESYEGSPASQGILQYDMWNVNPTDLWDWAELKEKIAKHG 591
>SPBC21.05c |ral2||Ras guanyl-nucleotide exchange factor Ral2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 611
Score = 26.6 bits (56), Expect = 9.1
Identities = 18/48 (37%), Positives = 26/48 (54%), Gaps = 9/48 (18%)
Frame = -1
Query: 652 PWSTISSDRHQSRYGVYS*YSTCSTFV----C-----SYSDANNVCNI 536
P+S+I S ++ Y YS + TCS F+ C SY A N+CN+
Sbjct: 491 PFSSIHSTGSRALYMPYS-FETCSAFLHYIYCGTLNGSYCTAKNLCNL 537
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 5,095,703
Number of Sequences: 5004
Number of extensions: 100110
Number of successful extensions: 277
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 257
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 277
length of database: 2,362,478
effective HSP length: 76
effective length of database: 1,982,174
effective search space used: 860263516
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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