SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 030725E6_D12_e572_08.seq
         (1514 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces pomb...    30   0.73 
SPBC354.13 |rga6||GTPase activating protein Rga6|Schizosaccharom...    29   1.3  
SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1 |Schi...    27   5.1  
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M...    27   6.8  
SPAC824.02 |||GPI inositol deacylase|Schizosaccharomyces pombe|c...    27   9.0  
SPBC947.11c |elg1||DNA replication factor C complex subunit Elg1...    27   9.0  
SPBC16A3.08c |||nuclear telomere cap complex subunit |Schizosacc...    27   9.0  

>SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 1334

 Score = 30.3 bits (65), Expect = 0.73
 Identities = 22/65 (33%), Positives = 31/65 (47%), Gaps = 3/65 (4%)
 Frame = +1

Query: 232 RHPYPANSRDLHQVVGPPCGEPTHAASSSTRQLLENPWA-PSGIGSTNNVASLLPLTF-- 402
           R P P  S  L      P   PTH   +S  + +E+  + PS  G++NN   ++PLT   
Sbjct: 59  RKPLPFRSTSLQPPSSQPPAPPTHQREASPVKNIEHSESFPSVFGTSNN-HQIVPLTLKD 117

Query: 403 GNSPG 417
           GN  G
Sbjct: 118 GNDFG 122


>SPBC354.13 |rga6||GTPase activating protein
           Rga6|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 733

 Score = 29.5 bits (63), Expect = 1.3
 Identities = 21/71 (29%), Positives = 33/71 (46%)
 Frame = +1

Query: 283 PCGEPTHAASSSTRQLLENPWAPSGIGSTNNVASLLPLTFGNSPGNVGYLGSSTDLLFSA 462
           PCG PT    S+  +  + P +   +      +++  + F + P N G LG S+  L S 
Sbjct: 136 PCGSPTKLIHSTLLEERDTPSSLEHVSFYLQESAVSEVRF-DKPSNNGPLGRSSLNLSSL 194

Query: 463 SITQ*NSEDSP 495
           S     S+DSP
Sbjct: 195 SHELQTSQDSP 205


>SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 374

 Score = 27.5 bits (58), Expect = 5.1
 Identities = 15/45 (33%), Positives = 23/45 (51%)
 Frame = +3

Query: 150 ITNPSIH*TTSTAGHRSPPKISTTTGLAPPVSSELPRPSPGRRST 284
           +++ S+  TTS++   SP   STTT  +P  SS     S    S+
Sbjct: 127 VSSSSVSSTTSSSSSSSPSSSSTTTTTSPSSSSSSSSSSSSSSSS 171


>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 309

 Score = 27.1 bits (57), Expect = 6.8
 Identities = 15/52 (28%), Positives = 22/52 (42%)
 Frame = +1

Query: 199 LPQRFPRPPVLRHPYPANSRDLHQVVGPPCGEPTHAASSSTRQLLENPWAPS 354
           +P + P PP+ + P  AN+        PP G   +  S S +     P  PS
Sbjct: 197 MPPKVPPPPLSQAPV-ANTSSRPSSFAPPAGHAPNVTSESPKFPNRGPSIPS 247


>SPAC824.02 |||GPI inositol deacylase|Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 1142

 Score = 26.6 bits (56), Expect = 9.0
 Identities = 13/36 (36%), Positives = 16/36 (44%)
 Frame = -3

Query: 669  LPKWYELRLGRYMSMQTHSRVGFYLILTFFIYHKNG 562
            L  +Y L  G       H+ VG Y     F+Y KNG
Sbjct: 1079 LLSFYSLIYGAEKPYLIHNVVGLYFFWLLFLYAKNG 1114


>SPBC947.11c |elg1||DNA replication factor C complex subunit
           Elg1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 920

 Score = 26.6 bits (56), Expect = 9.0
 Identities = 22/82 (26%), Positives = 34/82 (41%)
 Frame = +1

Query: 241 YPANSRDLHQVVGPPCGEPTHAASSSTRQLLENPWAPSGIGSTNNVASLLPLTFGNSPGN 420
           +P  S D +    PP      A  S T  L+EN  +   + S+  V+ L      +SP N
Sbjct: 110 HPKLSNDTNSTAFPPAKSSGEA--SDTNILVENINSQETVNSSPLVSELHYSNLADSPSN 167

Query: 421 VGYLGSSTDLLFSASITQ*NSE 486
           +    +S    F +   + NSE
Sbjct: 168 LRNTVTSMHPFFMSKSVKKNSE 189


>SPBC16A3.08c |||nuclear telomere cap complex subunit
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 284

 Score = 26.6 bits (56), Expect = 9.0
 Identities = 18/59 (30%), Positives = 28/59 (47%)
 Frame = +3

Query: 90  SPRQQKKLKKIVHIIEQIMHITNPSIH*TTSTAGHRSPPKISTTTGLAPPVSSELPRPS 266
           S  ++ K KK++  IEQ      P+     + A  R P + ++ T  APP  SE   P+
Sbjct: 225 SAAKESKPKKVLLDIEQTF-TARPARGGRPNRAPRRGPSETASKTQQAPPTLSETDFPA 282


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 5,067,553
Number of Sequences: 5004
Number of extensions: 101991
Number of successful extensions: 213
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 197
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 213
length of database: 2,362,478
effective HSP length: 76
effective length of database: 1,982,174
effective search space used: 848370472
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -