Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030725E6_D10_e556_08.seq
(1476 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_Q16T78 Cluster: Rer1 protein; n=2; Culicidae|Rep: Rer1 ... 75 4e-12
UniRef50_O15258 Cluster: Protein RER1; n=71; Eukaryota|Rep: Prot... 71 6e-11
UniRef50_Q4P895 Cluster: Putative uncharacterized protein; n=1; ... 58 6e-07
UniRef50_Q10358 Cluster: Protein rer1; n=10; Ascomycota|Rep: Pro... 56 2e-06
UniRef50_Q9ZWI7 Cluster: Protein RER1C; n=4; core eudicotyledons... 54 1e-05
UniRef50_Q54D10 Cluster: Putative uncharacterized protein; n=1; ... 53 2e-05
UniRef50_A2YTT4 Cluster: Putative uncharacterized protein; n=4; ... 52 4e-05
UniRef50_Q5KPE9 Cluster: ER to Golgi transport-related protein, ... 52 4e-05
UniRef50_Q00W53 Cluster: Golgi proteins involved in ER retention... 52 5e-05
UniRef50_A2ZNC4 Cluster: Putative uncharacterized protein; n=1; ... 50 2e-04
UniRef50_P25560 Cluster: Protein RER1; n=8; Ascomycota|Rep: Prot... 48 6e-04
UniRef50_Q86FF2 Cluster: Clone ZZD1243 mRNA sequence; n=2; Schis... 48 8e-04
UniRef50_O48671 Cluster: Protein RER1B; n=11; Magnoliophyta|Rep:... 47 0.001
UniRef50_Q7RNJ3 Cluster: Drosophila melanogaster RE24638p; n=7; ... 46 0.003
UniRef50_A2FCE5 Cluster: Putative uncharacterized protein; n=1; ... 39 0.38
UniRef50_A2F502 Cluster: Formin Homology 2 Domain containing pro... 38 0.88
UniRef50_Q8SRC9 Cluster: PROTEIN INVOLVED IN RETRIEVAL OF ER MEM... 37 1.5
UniRef50_Q02202 Cluster: Protein ECM9; n=2; Saccharomyces cerevi... 36 2.0
UniRef50_Q2AGZ0 Cluster: Hydrophobe/amphiphile efflux-3 HAE3 pre... 36 3.6
UniRef50_Q8IU42 Cluster: Formin homology protein A; n=2; Dictyos... 35 4.7
UniRef50_A0CS82 Cluster: Chromosome undetermined scaffold_26, wh... 35 6.2
UniRef50_UPI0000499927 Cluster: RER1 protein; n=1; Entamoeba his... 34 8.2
>UniRef50_Q16T78 Cluster: Rer1 protein; n=2; Culicidae|Rep: Rer1
protein - Aedes aegypti (Yellowfever mosquito)
Length = 186
Score = 75.4 bits (177), Expect = 4e-12
Identities = 31/45 (68%), Positives = 37/45 (82%)
Frame = -2
Query: 380 MCFVSIFFIFYLQGWYIITYALGIYHLNLFIAFLTPKIDPAMDFD 246
+ + + +F QGWYI+TYALGIYHLNLFIAFLTPKIDPA+D D
Sbjct: 45 LILIFLLRVFTKQGWYIVTYALGIYHLNLFIAFLTPKIDPALDLD 89
>UniRef50_O15258 Cluster: Protein RER1; n=71; Eukaryota|Rep: Protein
RER1 - Homo sapiens (Human)
Length = 196
Score = 71.3 bits (167), Expect = 6e-11
Identities = 28/42 (66%), Positives = 37/42 (88%)
Frame = -2
Query: 380 MCFVSIFFIFYLQGWYIITYALGIYHLNLFIAFLTPKIDPAM 255
+ FV + ++ LQGWYI+TYALGIYHLNLFIAFL+PK+DP++
Sbjct: 50 LSFVYMIRVYLLQGWYIVTYALGIYHLNLFIAFLSPKVDPSL 91
>UniRef50_Q4P895 Cluster: Putative uncharacterized protein; n=1;
Ustilago maydis|Rep: Putative uncharacterized protein -
Ustilago maydis (Smut fungus)
Length = 364
Score = 58.0 bits (134), Expect = 6e-07
Identities = 25/46 (54%), Positives = 33/46 (71%)
Frame = -2
Query: 383 SMCFVSIFFIFYLQGWYIITYALGIYHLNLFIAFLTPKIDPAMDFD 246
++ F+ + I QGWYI+ YAL IY LNLF+AFLTPK DP+ + D
Sbjct: 148 ALLFIFMLRIVLSQGWYIVCYALFIYLLNLFLAFLTPKFDPSYEAD 193
>UniRef50_Q10358 Cluster: Protein rer1; n=10; Ascomycota|Rep:
Protein rer1 - Schizosaccharomyces pombe (Fission yeast)
Length = 184
Score = 56.0 bits (129), Expect = 2e-06
Identities = 24/42 (57%), Positives = 33/42 (78%), Gaps = 2/42 (4%)
Frame = -2
Query: 371 VSIFFIFYL--QGWYIITYALGIYHLNLFIAFLTPKIDPAMD 252
+++FFI L +GWYI+ Y L IY LNLF+AFLTPK DP+++
Sbjct: 44 IALFFIRILLVRGWYIVCYTLAIYLLNLFLAFLTPKFDPSVE 85
>UniRef50_Q9ZWI7 Cluster: Protein RER1C; n=4; core
eudicotyledons|Rep: Protein RER1C - Arabidopsis thaliana
(Mouse-ear cress)
Length = 212
Score = 53.6 bits (123), Expect = 1e-05
Identities = 23/42 (54%), Positives = 34/42 (80%), Gaps = 2/42 (4%)
Frame = -2
Query: 380 MCFVSIFFI--FYLQGWYIITYALGIYHLNLFIAFLTPKIDP 261
+C V I+ + ++++G+YIITYA+GIY LNL IAFL+P+ DP
Sbjct: 67 LCVVLIYIVRVYFVEGFYIITYAIGIYLLNLIIAFLSPQEDP 108
>UniRef50_Q54D10 Cluster: Putative uncharacterized protein; n=1;
Dictyostelium discoideum AX4|Rep: Putative
uncharacterized protein - Dictyostelium discoideum AX4
Length = 188
Score = 52.8 bits (121), Expect = 2e-05
Identities = 25/53 (47%), Positives = 34/53 (64%)
Frame = -2
Query: 341 GWYIITYALGIYHLNLFIAFLTPKIDPAMDFDGKSSC*YDIDRVGFS*KQYLR 183
GWY+ITYALGI+ L FIAFL+PK DP ++ D S ++R K ++R
Sbjct: 61 GWYVITYALGIFLLTRFIAFLSPKWDPELEEDSGDSLPTTLNRNDDEAKPFIR 113
>UniRef50_A2YTT4 Cluster: Putative uncharacterized protein; n=4;
Oryza sativa|Rep: Putative uncharacterized protein -
Oryza sativa subsp. indica (Rice)
Length = 253
Score = 52.0 bits (119), Expect = 4e-05
Identities = 23/47 (48%), Positives = 32/47 (68%)
Frame = -2
Query: 401 RLL*FTSMCFVSIFFIFYLQGWYIITYALGIYHLNLFIAFLTPKIDP 261
R L F + +++ G+YI+TYALGIY LNL IAFL+P++DP
Sbjct: 58 RWLAFAGVAAAYALRVWFAGGYYIVTYALGIYILNLLIAFLSPQVDP 104
>UniRef50_Q5KPE9 Cluster: ER to Golgi transport-related protein,
putative; n=2; Dikarya|Rep: ER to Golgi
transport-related protein, putative - Cryptococcus
neoformans (Filobasidiella neoformans)
Length = 265
Score = 52.0 bits (119), Expect = 4e-05
Identities = 21/33 (63%), Positives = 26/33 (78%)
Frame = -2
Query: 344 QGWYIITYALGIYHLNLFIAFLTPKIDPAMDFD 246
QGWYI+ YAL IY LNLF+AFL P+ DP++ D
Sbjct: 113 QGWYIVCYALAIYILNLFLAFLQPRFDPSLAED 145
>UniRef50_Q00W53 Cluster: Golgi proteins involved in ER retention;
n=1; Ostreococcus tauri|Rep: Golgi proteins involved in
ER retention - Ostreococcus tauri
Length = 206
Score = 51.6 bits (118), Expect = 5e-05
Identities = 21/43 (48%), Positives = 31/43 (72%)
Frame = -2
Query: 389 FTSMCFVSIFFIFYLQGWYIITYALGIYHLNLFIAFLTPKIDP 261
+ + FV ++L+G+YI+TY LGIY+LNL I FL+P+ DP
Sbjct: 50 YAFVAFVYAVRAYFLKGYYIVTYGLGIYNLNLLIGFLSPQRDP 92
>UniRef50_A2ZNC4 Cluster: Putative uncharacterized protein; n=1;
Oryza sativa (japonica cultivar-group)|Rep: Putative
uncharacterized protein - Oryza sativa subsp. japonica
(Rice)
Length = 202
Score = 49.6 bits (113), Expect = 2e-04
Identities = 19/32 (59%), Positives = 26/32 (81%)
Frame = -2
Query: 356 IFYLQGWYIITYALGIYHLNLFIAFLTPKIDP 261
++ +QG+YI+TY LGIY LNL I FL+P +DP
Sbjct: 119 VYLVQGFYIVTYGLGIYLLNLLIGFLSPMVDP 150
>UniRef50_P25560 Cluster: Protein RER1; n=8; Ascomycota|Rep: Protein
RER1 - Saccharomyces cerevisiae (Baker's yeast)
Length = 188
Score = 48.0 bits (109), Expect = 6e-04
Identities = 18/37 (48%), Positives = 28/37 (75%)
Frame = -2
Query: 344 QGWYIITYALGIYHLNLFIAFLTPKIDPAMDFDGKSS 234
+GWY+I Y LG++ LN F+AFLTPK D ++ D +++
Sbjct: 61 EGWYVICYGLGLFLLNQFLAFLTPKFDMSLQQDEENN 97
>UniRef50_Q86FF2 Cluster: Clone ZZD1243 mRNA sequence; n=2;
Schistosoma japonicum|Rep: Clone ZZD1243 mRNA sequence -
Schistosoma japonicum (Blood fluke)
Length = 196
Score = 47.6 bits (108), Expect = 8e-04
Identities = 21/45 (46%), Positives = 33/45 (73%), Gaps = 1/45 (2%)
Frame = -2
Query: 389 FTSMCF-VSIFFIFYLQGWYIITYALGIYHLNLFIAFLTPKIDPA 258
F M F + + I +QG++I++Y L IY L+LFI+F++PK+DPA
Sbjct: 43 FALMLFAIYVLRIASIQGFHIVSYTLAIYLLSLFISFISPKVDPA 87
>UniRef50_O48671 Cluster: Protein RER1B; n=11; Magnoliophyta|Rep:
Protein RER1B - Arabidopsis thaliana (Mouse-ear cress)
Length = 195
Score = 47.2 bits (107), Expect = 0.001
Identities = 18/35 (51%), Positives = 27/35 (77%)
Frame = -2
Query: 356 IFYLQGWYIITYALGIYHLNLFIAFLTPKIDPAMD 252
++ + G+YII+Y LGIY LNL I FL+P +DP ++
Sbjct: 55 VYSIHGFYIISYGLGIYLLNLLIGFLSPLVDPELE 89
>UniRef50_Q7RNJ3 Cluster: Drosophila melanogaster RE24638p; n=7;
Plasmodium|Rep: Drosophila melanogaster RE24638p -
Plasmodium yoelii yoelii
Length = 211
Score = 45.6 bits (103), Expect = 0.003
Identities = 17/48 (35%), Positives = 32/48 (66%)
Frame = -2
Query: 416 VFSKSRLL*FTSMCFVSIFFIFYLQGWYIITYALGIYHLNLFIAFLTP 273
++ K+R + + + ++Y+ G+Y+++YAL I+ LNLF+ FLTP
Sbjct: 28 LYLKTRWFSLLGLFIIYVLRVYYVTGFYVVSYALSIFLLNLFLRFLTP 75
>UniRef50_A2FCE5 Cluster: Putative uncharacterized protein; n=1;
Trichomonas vaginalis G3|Rep: Putative uncharacterized
protein - Trichomonas vaginalis G3
Length = 178
Score = 38.7 bits (86), Expect = 0.38
Identities = 17/75 (22%), Positives = 39/75 (52%)
Frame = -2
Query: 479 RVLLNYKLQTLNYQNL*CTFNVFSKSRLL*FTSMCFVSIFFIFYLQGWYIITYALGIYHL 300
+++++ + + +++L + R F M F+ + +F +Y I Y +G+Y L
Sbjct: 11 KIVIDKQEALMRFESLLYQIKPYLWQRWCAFGFMMFLFLLRVFTWHAYYYIVYIIGLYLL 70
Query: 299 NLFIAFLTPKIDPAM 255
+ + F++PK DP +
Sbjct: 71 SCVVEFISPKRDPEL 85
>UniRef50_A2F502 Cluster: Formin Homology 2 Domain containing protein;
n=2; Trichomonas vaginalis G3|Rep: Formin Homology 2
Domain containing protein - Trichomonas vaginalis G3
Length = 1139
Score = 37.5 bits (83), Expect = 0.88
Identities = 22/61 (36%), Positives = 23/61 (37%)
Frame = -3
Query: 1465 GGXPXPXXXXPXAGGXPKRXGPRXRXLKXXXPXVFXXPAXKGXXGXK*PXPPPGXGGXGP 1286
G P P P AGG P P + P V P G G P PPPG G P
Sbjct: 611 GLVPPPPPPPPGAGGIPPPPPPPGAGIPPPPPGVPGIPPPPGAPGL--PPPPPGVPGIPP 668
Query: 1285 P 1283
P
Sbjct: 669 P 669
>UniRef50_Q8SRC9 Cluster: PROTEIN INVOLVED IN RETRIEVAL OF ER
MEMBRANE PROTEINS FROM THE EARLY GOLGI COMPARTMENT; n=1;
Encephalitozoon cuniculi|Rep: PROTEIN INVOLVED IN
RETRIEVAL OF ER MEMBRANE PROTEINS FROM THE EARLY GOLGI
COMPARTMENT - Encephalitozoon cuniculi
Length = 166
Score = 36.7 bits (81), Expect = 1.5
Identities = 19/38 (50%), Positives = 25/38 (65%), Gaps = 6/38 (15%)
Frame = -2
Query: 365 IFFIFY-LQGW-----YIITYALGIYHLNLFIAFLTPK 270
+ F+FY ++ W Y+ITY LGIY L+ I FLTPK
Sbjct: 27 VLFLFYCIRIWSTGAFYLITYCLGIYLLHALILFLTPK 64
>UniRef50_Q02202 Cluster: Protein ECM9; n=2; Saccharomyces
cerevisiae|Rep: Protein ECM9 - Saccharomyces cerevisiae
(Baker's yeast)
Length = 377
Score = 36.3 bits (80), Expect = 2.0
Identities = 19/64 (29%), Positives = 34/64 (53%), Gaps = 2/64 (3%)
Frame = -2
Query: 851 YPSKYCLKALTKINISLKIVMSFYLVCTRH--FNLHLHTFYLHSLNNSRSINQNLSMSFI 678
+P CL +TK NI+L+I M L+ R+ N ++ ++ +NN+ + + S +
Sbjct: 289 WPPYLCLLQITKFNITLRIEMDSVLLTWRNEILNFEENSGHIKMINNTPIVPEKFSNDLL 348
Query: 677 TKVN 666
T VN
Sbjct: 349 TSVN 352
>UniRef50_Q2AGZ0 Cluster: Hydrophobe/amphiphile efflux-3 HAE3
precursor; n=1; Halothermothrix orenii H 168|Rep:
Hydrophobe/amphiphile efflux-3 HAE3 precursor -
Halothermothrix orenii H 168
Length = 764
Score = 35.5 bits (78), Expect = 3.6
Identities = 20/43 (46%), Positives = 28/43 (65%), Gaps = 4/43 (9%)
Frame = -3
Query: 442 TKICNVLLMFSVKADYYNLLPCVL--LASFLFFIYRD--GILL 326
T + N +L S+KAD Y LLP VL +A LF ++RD G++L
Sbjct: 194 TPVLNNVLAESMKADLYKLLPLVLFIIALILFLVFRDFKGVIL 236
>UniRef50_Q8IU42 Cluster: Formin homology protein A; n=2;
Dictyostelium discoideum|Rep: Formin homology protein A -
Dictyostelium discoideum (Slime mold)
Length = 1218
Score = 35.1 bits (77), Expect = 4.7
Identities = 21/62 (33%), Positives = 21/62 (33%)
Frame = -3
Query: 1468 GGGXPXPXXXXPXAGGXPKRXGPRXRXLKXXXPXVFXXPAXKGXXGXK*PXPPPGXGGXG 1289
GGG P P P GG P P P P G P PPPG G
Sbjct: 675 GGGPPPPPPPPPMTGGGPPPPPPPPPMTGGGPPP----PPPPPGGGPPPPPPPPGAKAGG 730
Query: 1288 PP 1283
PP
Sbjct: 731 PP 732
>UniRef50_A0CS82 Cluster: Chromosome undetermined scaffold_26, whole
genome shotgun sequence; n=1; Paramecium
tetraurelia|Rep: Chromosome undetermined scaffold_26,
whole genome shotgun sequence - Paramecium tetraurelia
Length = 193
Score = 34.7 bits (76), Expect = 6.2
Identities = 18/59 (30%), Positives = 30/59 (50%)
Frame = -2
Query: 449 LNYQNL*CTFNVFSKSRLL*FTSMCFVSIFFIFYLQGWYIITYALGIYHLNLFIAFLTP 273
+NYQ + ++ KSR + + + I + G+YI+TY +Y L L I + TP
Sbjct: 21 VNYQKKIDRYILYKKSRWFFNLLLMLLYAYRIQNIGGFYIVTYIYCVYQLQLLIDYFTP 79
>UniRef50_UPI0000499927 Cluster: RER1 protein; n=1; Entamoeba
histolytica HM-1:IMSS|Rep: RER1 protein - Entamoeba
histolytica HM-1:IMSS
Length = 174
Score = 34.3 bits (75), Expect = 8.2
Identities = 20/48 (41%), Positives = 28/48 (58%), Gaps = 1/48 (2%)
Frame = -2
Query: 413 FSKSRLL*FTSMCFVSIFF-IFYLQGWYIITYALGIYHLNLFIAFLTP 273
F+K+R F +C V + + + +Y+ YA GIY L FIAFLTP
Sbjct: 33 FTKTRWSLFI-ICLVWFMYRMVHYHKYYVYAYASGIYILFQFIAFLTP 79
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,257,653,067
Number of Sequences: 1657284
Number of extensions: 24374530
Number of successful extensions: 50609
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 43143
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 50001
length of database: 575,637,011
effective HSP length: 104
effective length of database: 403,279,475
effective search space used: 156069156825
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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