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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 030725E6_D06_e524_08.seq
         (1528 letters)

Database: uniref50 
           1,657,284 sequences; 575,637,011 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_P91682 Cluster: Protein smoothened precursor; n=6; Soph...    56   2e-06
UniRef50_Q175G5 Cluster: Putative uncharacterized protein; n=3; ...    50   1e-04
UniRef50_Q7PPM7 Cluster: ENSANGP00000013048; n=1; Anopheles gamb...    50   2e-04
UniRef50_UPI0000D56D95 Cluster: PREDICTED: similar to CG11561-PA...    40   0.13 
UniRef50_UPI0000F2E8E6 Cluster: PREDICTED: hypothetical protein;...    37   1.6  
UniRef50_UPI000155C0BA Cluster: PREDICTED: similar to chromosome...    36   3.7  
UniRef50_Q3JVE2 Cluster: Putative uncharacterized protein; n=3; ...    36   3.7  
UniRef50_UPI0000F32FE2 Cluster: UPI0000F32FE2 related cluster; n...    35   4.9  
UniRef50_A3SHS6 Cluster: Transcriptional Regulator, TetR family ...    35   6.5  
UniRef50_Q4H2S9 Cluster: Smoothened; n=1; Ciona intestinalis|Rep...    35   6.5  
UniRef50_UPI0000EBE059 Cluster: PREDICTED: hypothetical protein;...    34   8.6  

>UniRef50_P91682 Cluster: Protein smoothened precursor; n=6;
           Sophophora|Rep: Protein smoothened precursor -
           Drosophila melanogaster (Fruit fly)
          Length = 1036

 Score = 56.4 bits (130), Expect = 2e-06
 Identities = 30/49 (61%), Positives = 34/49 (69%), Gaps = 1/49 (2%)
 Frame = +3

Query: 390 LRHVSVESRXNXLDXQLSVKIAEMKTKVA-RRRNKHGKAKRKTRAVXTR 533
           +RHVSVESR N +D Q+SVKIAEMKTKVA R R KHG +    R    R
Sbjct: 676 VRHVSVESRRNSVDSQVSVKIAEMKTKVASRSRGKHGGSSSNRRTQRRR 724



 Score = 39.5 bits (88), Expect = 0.23
 Identities = 26/74 (35%), Positives = 39/74 (52%)
 Frame = +2

Query: 86  KHELIXXXXXXXXXXXXXXXLSISLNGSRXDPVGLCIDHTSPIDYPEDAKHESGELSSSW 265
           KH++I               LSI+L  +  DPVGL  D  + ++       E+ ++SS+W
Sbjct: 583 KHKVIAQTWAKRKDFEDKGRLSITLYNTHTDPVGLNFD-VNDLN-----SSETNDISSTW 636

Query: 266 AANLPRFVRRRDAL 307
           AA LP+ V+RR AL
Sbjct: 637 AAYLPQCVKRRMAL 650


>UniRef50_Q175G5 Cluster: Putative uncharacterized protein; n=3;
           Endopterygota|Rep: Putative uncharacterized protein -
           Aedes aegypti (Yellowfever mosquito)
          Length = 967

 Score = 50.4 bits (115), Expect = 1e-04
 Identities = 24/31 (77%), Positives = 27/31 (87%)
 Frame = +3

Query: 390 LRHVSVESRXNXLDXQLSVKIAEMKTKVARR 482
           +RHVSVESR N +D Q+SVKIAEMKTKVA R
Sbjct: 624 VRHVSVESRRNSIDSQVSVKIAEMKTKVASR 654



 Score = 46.8 bits (106), Expect = 0.002
 Identities = 31/77 (40%), Positives = 37/77 (48%)
 Frame = +2

Query: 77  RAHKHELIXXXXXXXXXXXXXXXLSISLNGSRXDPVGLCIDHTSPIDYPEDAKHESGELS 256
           +  KH++I               LSIS + S  DPVGL  D  S I         S + S
Sbjct: 532 KLQKHKVIAQAFAKRKEFQNQGRLSISFHNSHTDPVGLKFDINSAIG--------SHDFS 583

Query: 257 SSWAANLPRFVRRRDAL 307
           S+WA NLPRFV RR AL
Sbjct: 584 STWANNLPRFVNRRYAL 600


>UniRef50_Q7PPM7 Cluster: ENSANGP00000013048; n=1; Anopheles gambiae
           str. PEST|Rep: ENSANGP00000013048 - Anopheles gambiae
           str. PEST
          Length = 1038

 Score = 49.6 bits (113), Expect = 2e-04
 Identities = 23/34 (67%), Positives = 29/34 (85%)
 Frame = +3

Query: 390 LRHVSVESRXNXLDXQLSVKIAEMKTKVARRRNK 491
           +RHVSVESR N +D Q+SVKIAE+KTKVA R ++
Sbjct: 646 VRHVSVESRRNSIDSQVSVKIAEVKTKVASRSSR 679



 Score = 48.8 bits (111), Expect = 4e-04
 Identities = 32/77 (41%), Positives = 38/77 (49%)
 Frame = +2

Query: 77  RAHKHELIXXXXXXXXXXXXXXXLSISLNGSRXDPVGLCIDHTSPIDYPEDAKHESGELS 256
           +  KH++I               LSIS + S  DPVGL  D TS I         S + S
Sbjct: 554 KLQKHKVIAQAFAKRKEFQNQGRLSISFHNSHTDPVGLKFDITSAIG--------SHDFS 605

Query: 257 SSWAANLPRFVRRRDAL 307
           S+WA NLPRFV RR AL
Sbjct: 606 STWANNLPRFVNRRCAL 622


>UniRef50_UPI0000D56D95 Cluster: PREDICTED: similar to CG11561-PA;
           n=1; Tribolium castaneum|Rep: PREDICTED: similar to
           CG11561-PA - Tribolium castaneum
          Length = 846

 Score = 40.3 bits (90), Expect = 0.13
 Identities = 24/62 (38%), Positives = 35/62 (56%)
 Frame = +3

Query: 390 LRHVSVESRXNXLDXQLSVKIAEMKTKVARRRNKHGKAKRKTRAVXTRXESTPVHREPNK 569
           +RHVS+ESR N  D QLSV+I+E+  K  R+ N+  + +R      +R   T   RE + 
Sbjct: 634 VRHVSIESRCNSGDSQLSVQISEL--KATRKVNRRHRRRRHDFRRDSRLSKTGNKRESSA 691

Query: 570 PL 575
            L
Sbjct: 692 SL 693



 Score = 34.3 bits (75), Expect = 8.6
 Identities = 16/41 (39%), Positives = 23/41 (56%), Gaps = 1/41 (2%)
 Frame = +2

Query: 191 CIDHTSPIDYPEDAKHE-SGELSSSWAANLPRFVRRRDALV 310
           C ++T P+    D     S   S++WA NLPR + RR A+V
Sbjct: 573 CQNYTDPVGLHFDLNSAVSDSFSTAWAVNLPRLLNRRGAIV 613


>UniRef50_UPI0000F2E8E6 Cluster: PREDICTED: hypothetical protein;
           n=1; Monodelphis domestica|Rep: PREDICTED: hypothetical
           protein - Monodelphis domestica
          Length = 206

 Score = 36.7 bits (81), Expect = 1.6
 Identities = 18/49 (36%), Positives = 24/49 (48%)
 Frame = -3

Query: 230 RPPGSRWATCGRYRARPGPXGSRSAISTSVPAPRTPPCAGTRAGSARAC 84
           +PPG   +  GR   RPG   +  A + ++ APR  P AG   G  R C
Sbjct: 131 KPPGKPGSGPGRRSLRPGAEEAPVAEAPTLQAPRAEPMAGPAPGGGRRC 179


>UniRef50_UPI000155C0BA Cluster: PREDICTED: similar to chromosome 20
           open reading frame 118; n=1; Ornithorhynchus
           anatinus|Rep: PREDICTED: similar to chromosome 20 open
           reading frame 118 - Ornithorhynchus anatinus
          Length = 274

 Score = 35.5 bits (78), Expect = 3.7
 Identities = 23/62 (37%), Positives = 29/62 (46%), Gaps = 4/62 (6%)
 Frame = -3

Query: 227 PPGSRWATCGRYRARPGPXGSRSAISTSVPAPRTPP--CAGTRAGSAR--ACGRDESPSL 60
           P  + W  CG   ARP    +R  ++     PR PP  C   R G +R  ACGRDE+   
Sbjct: 173 PHAAPWGGCGVDSARPPRSNARPRLT-----PRGPPNLCPDARPGGSRPSACGRDEASRS 227

Query: 59  VP 54
            P
Sbjct: 228 EP 229


>UniRef50_Q3JVE2 Cluster: Putative uncharacterized protein; n=3;
           Burkholderia|Rep: Putative uncharacterized protein -
           Burkholderia pseudomallei (strain 1710b)
          Length = 1059

 Score = 35.5 bits (78), Expect = 3.7
 Identities = 18/39 (46%), Positives = 20/39 (51%), Gaps = 3/39 (7%)
 Frame = -3

Query: 188 ARPGPXGSRSAISTSVPAPRTPPCAG---TRAGSARACG 81
           ARP        +S S+PAPR PPCA    T   S R CG
Sbjct: 116 ARPPSRSLPRPLSASLPAPRHPPCASISLTSCNSVRTCG 154


>UniRef50_UPI0000F32FE2 Cluster: UPI0000F32FE2 related cluster; n=1;
           Bos taurus|Rep: UPI0000F32FE2 UniRef100 entry - Bos
           Taurus
          Length = 706

 Score = 35.1 bits (77), Expect = 4.9
 Identities = 22/56 (39%), Positives = 24/56 (42%)
 Frame = -3

Query: 230 RPPGSRWATCGRYRARPGPXGSRSAISTSVPAPRTPPCAGTRAGSARACGRDESPS 63
           R PG      GR   RPGP   RS      P+PR  P  G  A S    GR  SP+
Sbjct: 567 RTPGPHGQNAGR-AWRPGPV-VRSGAGPGGPSPRPVPRPGVLAASLHGAGRPRSPT 620


>UniRef50_A3SHS6 Cluster: Transcriptional Regulator, TetR family
           protein; n=4; Proteobacteria|Rep: Transcriptional
           Regulator, TetR family protein - Roseovarius nubinhibens
           ISM
          Length = 197

 Score = 34.7 bits (76), Expect = 6.5
 Identities = 19/39 (48%), Positives = 20/39 (51%)
 Frame = +1

Query: 88  ARADPARVPAQGGVRGAGTLVDIAERLPXGPGRALYRPH 204
           A AD A    + G RG G L   AE LP  PGRA  R H
Sbjct: 91  AYADHANFAYERGFRGCGLLNAAAELLPGDPGRAAVRRH 129


>UniRef50_Q4H2S9 Cluster: Smoothened; n=1; Ciona intestinalis|Rep:
           Smoothened - Ciona intestinalis (Transparent sea squirt)
          Length = 691

 Score = 34.7 bits (76), Expect = 6.5
 Identities = 17/44 (38%), Positives = 22/44 (50%)
 Frame = +2

Query: 200 HTSPIDYPEDAKHESGELSSSWAANLPRFVRRRDALVLPTHTHH 331
           H  P+    D    S E+SSSW  N+P  V+RR  + LP    H
Sbjct: 565 HQDPLGMNFDLHSVSQEMSSSWVRNVPNMVKRRGGM-LPMEQPH 607


>UniRef50_UPI0000EBE059 Cluster: PREDICTED: hypothetical protein;
           n=2; Bos taurus|Rep: PREDICTED: hypothetical protein -
           Bos taurus
          Length = 834

 Score = 34.3 bits (75), Expect = 8.6
 Identities = 20/50 (40%), Positives = 27/50 (54%), Gaps = 1/50 (2%)
 Frame = -3

Query: 200 GRYRARPGPXGSR-SAISTSVPAPRTPPCAGTRAGSARACGRDESPSLVP 54
           GR R+      +R SA+S     P TPP AG R    RA GR+ +P+ +P
Sbjct: 736 GRSRSTRREGEARASAVSEDPRPPLTPPEAGDRRPKVRARGRNPAPAFLP 785


  Database: uniref50
    Posted date:  Oct 5, 2007 11:19 AM
  Number of letters in database: 575,637,011
  Number of sequences in database:  1,657,284
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 606,319,326
Number of Sequences: 1657284
Number of extensions: 9263492
Number of successful extensions: 38226
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 34983
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 38085
length of database: 575,637,011
effective HSP length: 104
effective length of database: 403,279,475
effective search space used: 162924907900
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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