BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= 030725E6_D06_e524_08.seq (1528 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC15A10.15 |sgo2||shugoshin Sgo2|Schizosaccharomyces pombe|chr... 28 3.9 SPAC23C4.10 |sec2||guanyl-nucleotide exchange factor Sec2 |Schiz... 27 6.8 >SPAC15A10.15 |sgo2||shugoshin Sgo2|Schizosaccharomyces pombe|chr 1|||Manual Length = 647 Score = 27.9 bits (59), Expect = 3.9 Identities = 15/36 (41%), Positives = 17/36 (47%) Frame = +3 Query: 465 TKVARRRNKHGKAKRKTRAVXTRXESTPVHREPNKP 572 TK RR K +RK R T EST V+ KP Sbjct: 534 TKTQSRRETKVKRRRKARIQETSEESTVVNEPNEKP 569 >SPAC23C4.10 |sec2||guanyl-nucleotide exchange factor Sec2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 527 Score = 27.1 bits (57), Expect = 6.8 Identities = 15/41 (36%), Positives = 20/41 (48%) Frame = -3 Query: 167 SRSAISTSVPAPRTPPCAGTRAGSARACGRDESPSLVPNXL 45 SRSA STS TPP +AR+ ++PS N + Sbjct: 336 SRSAYSTSANYSNTPPRNTVTPSTARSSTLSQNPSSSSNSI 376 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,474,137 Number of Sequences: 5004 Number of extensions: 33830 Number of successful extensions: 99 Number of sequences better than 10.0: 2 Number of HSP's better than 10.0 without gapping: 94 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 99 length of database: 2,362,478 effective HSP length: 76 effective length of database: 1,982,174 effective search space used: 856299168 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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