Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030725E6_D06_e524_08.seq
(1528 letters)
Database: fruitfly
53,049 sequences; 24,988,368 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
U87613-1|AAC33180.1| 1036|Drosophila melanogaster seven-pass mem... 56 1e-07
BT003246-1|AAO25003.1| 1036|Drosophila melanogaster LD34308p pro... 56 1e-07
AF030334-1|AAB84275.1| 1036|Drosophila melanogaster smoothened p... 56 1e-07
AE014134-73|AAF51518.2| 1036|Drosophila melanogaster CG11561-PA ... 56 1e-07
AY089617-1|AAL90355.1| 958|Drosophila melanogaster RE32881p pro... 33 1.4
AE014298-1748|AAF48140.5| 1040|Drosophila melanogaster CG32656-P... 33 1.4
>U87613-1|AAC33180.1| 1036|Drosophila melanogaster seven-pass
membrane protein protein.
Length = 1036
Score = 56.4 bits (130), Expect = 1e-07
Identities = 30/49 (61%), Positives = 34/49 (69%), Gaps = 1/49 (2%)
Frame = +3
Query: 390 LRHVSVESRXNXLDXQLSVKIAEMKTKVA-RRRNKHGKAKRKTRAVXTR 533
+RHVSVESR N +D Q+SVKIAEMKTKVA R R KHG + R R
Sbjct: 676 VRHVSVESRRNSVDSQVSVKIAEMKTKVASRSRGKHGGSSSNRRTQRRR 724
Score = 39.5 bits (88), Expect = 0.012
Identities = 26/74 (35%), Positives = 39/74 (52%)
Frame = +2
Query: 86 KHELIXXXXXXXXXXXXXXXLSISLNGSRXDPVGLCIDHTSPIDYPEDAKHESGELSSSW 265
KH++I LSI+L + DPVGL D + ++ E+ ++SS+W
Sbjct: 583 KHKVIAQTWAKRKDFEDKGRLSITLYNTHTDPVGLNFD-VNDLN-----SSETNDISSTW 636
Query: 266 AANLPRFVRRRDAL 307
AA LP+ V+RR AL
Sbjct: 637 AAYLPQCVKRRMAL 650
>BT003246-1|AAO25003.1| 1036|Drosophila melanogaster LD34308p
protein.
Length = 1036
Score = 56.4 bits (130), Expect = 1e-07
Identities = 30/49 (61%), Positives = 34/49 (69%), Gaps = 1/49 (2%)
Frame = +3
Query: 390 LRHVSVESRXNXLDXQLSVKIAEMKTKVA-RRRNKHGKAKRKTRAVXTR 533
+RHVSVESR N +D Q+SVKIAEMKTKVA R R KHG + R R
Sbjct: 676 VRHVSVESRRNSVDSQVSVKIAEMKTKVASRSRGKHGGSSSNRRTQRRR 724
Score = 39.5 bits (88), Expect = 0.012
Identities = 26/74 (35%), Positives = 39/74 (52%)
Frame = +2
Query: 86 KHELIXXXXXXXXXXXXXXXLSISLNGSRXDPVGLCIDHTSPIDYPEDAKHESGELSSSW 265
KH++I LSI+L + DPVGL D + ++ E+ ++SS+W
Sbjct: 583 KHKVIAQTWAKRKDFEDKGRLSITLYNTHTDPVGLNFD-VNDLN-----SSETNDISSTW 636
Query: 266 AANLPRFVRRRDAL 307
AA LP+ V+RR AL
Sbjct: 637 AAYLPQCVKRRMAL 650
>AF030334-1|AAB84275.1| 1036|Drosophila melanogaster smoothened
protein.
Length = 1036
Score = 56.4 bits (130), Expect = 1e-07
Identities = 30/49 (61%), Positives = 34/49 (69%), Gaps = 1/49 (2%)
Frame = +3
Query: 390 LRHVSVESRXNXLDXQLSVKIAEMKTKVA-RRRNKHGKAKRKTRAVXTR 533
+RHVSVESR N +D Q+SVKIAEMKTKVA R R KHG + R R
Sbjct: 676 VRHVSVESRRNSVDSQVSVKIAEMKTKVASRSRGKHGGSSSNRRTQRRR 724
Score = 39.5 bits (88), Expect = 0.012
Identities = 26/74 (35%), Positives = 39/74 (52%)
Frame = +2
Query: 86 KHELIXXXXXXXXXXXXXXXLSISLNGSRXDPVGLCIDHTSPIDYPEDAKHESGELSSSW 265
KH++I LSI+L + DPVGL D + ++ E+ ++SS+W
Sbjct: 583 KHKVIAQTWAKRKDFEDKGRLSITLYNTHTDPVGLNFD-VNDLN-----SSETNDISSTW 636
Query: 266 AANLPRFVRRRDAL 307
AA LP+ V+RR AL
Sbjct: 637 AAYLPQCVKRRMAL 650
>AE014134-73|AAF51518.2| 1036|Drosophila melanogaster CG11561-PA
protein.
Length = 1036
Score = 56.4 bits (130), Expect = 1e-07
Identities = 30/49 (61%), Positives = 34/49 (69%), Gaps = 1/49 (2%)
Frame = +3
Query: 390 LRHVSVESRXNXLDXQLSVKIAEMKTKVA-RRRNKHGKAKRKTRAVXTR 533
+RHVSVESR N +D Q+SVKIAEMKTKVA R R KHG + R R
Sbjct: 676 VRHVSVESRRNSVDSQVSVKIAEMKTKVASRSRGKHGGSSSNRRTQRRR 724
Score = 39.5 bits (88), Expect = 0.012
Identities = 26/74 (35%), Positives = 39/74 (52%)
Frame = +2
Query: 86 KHELIXXXXXXXXXXXXXXXLSISLNGSRXDPVGLCIDHTSPIDYPEDAKHESGELSSSW 265
KH++I LSI+L + DPVGL D + ++ E+ ++SS+W
Sbjct: 583 KHKVIAQTWAKRKDFEDKGRLSITLYNTHTDPVGLNFD-VNDLN-----SSETNDISSTW 636
Query: 266 AANLPRFVRRRDAL 307
AA LP+ V+RR AL
Sbjct: 637 AAYLPQCVKRRMAL 650
>AY089617-1|AAL90355.1| 958|Drosophila melanogaster RE32881p
protein.
Length = 958
Score = 32.7 bits (71), Expect = 1.4
Identities = 17/45 (37%), Positives = 22/45 (48%)
Frame = -3
Query: 188 ARPGPXGSRSAISTSVPAPRTPPCAGTRAGSARACGRDESPSLVP 54
A PG S+ + PA +PP G+ AGS G D + S VP
Sbjct: 486 AAPGAPADGSSAAPGSPAEGSPPAGGSPAGSNVPAGGDPADSNVP 530
>AE014298-1748|AAF48140.5| 1040|Drosophila melanogaster CG32656-PA
protein.
Length = 1040
Score = 32.7 bits (71), Expect = 1.4
Identities = 17/45 (37%), Positives = 22/45 (48%)
Frame = -3
Query: 188 ARPGPXGSRSAISTSVPAPRTPPCAGTRAGSARACGRDESPSLVP 54
A PG S+ + PA +PP G+ AGS G D + S VP
Sbjct: 568 AAPGAPADGSSAAPGSPAEGSPPAGGSPAGSNVPAGGDPADSNVP 612
Database: fruitfly
Posted date: Oct 23, 2007 1:17 PM
Number of letters in database: 24,988,368
Number of sequences in database: 53,049
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 27,482,352
Number of Sequences: 53049
Number of extensions: 445343
Number of successful extensions: 1657
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 1536
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1653
length of database: 24,988,368
effective HSP length: 88
effective length of database: 20,320,056
effective search space used: 8534423520
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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