BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= 030725E6_D05_e516_07.seq (1548 letters) Database: bee 438 sequences; 146,343 total letters Searching......................................................done Score E Sequences producing significant alignments: (bits) Value AB047034-1|BAB64310.1| 1598|Apis mellifera mblk-1 protein. 27 0.57 AB204559-1|BAD89804.1| 832|Apis mellifera soluble guanylyl cycl... 23 5.3 >AB047034-1|BAB64310.1| 1598|Apis mellifera mblk-1 protein. Length = 1598 Score = 26.6 bits (56), Expect = 0.57 Identities = 12/50 (24%), Positives = 24/50 (48%) Frame = +2 Query: 281 RACLQCDRTRSRRDREQARPRQDPTETHQHPHRAREALQVQAGLPQARQG 430 R + + ++ ++Q + +Q + Q H+ARE V AG+ + G Sbjct: 1203 RNAAMVQQQQQQQQQQQQQQQQQQQQQQQQQHQAREREGVGAGIAETSAG 1252 Score = 22.6 bits (46), Expect = 9.3 Identities = 14/50 (28%), Positives = 24/50 (48%), Gaps = 5/50 (10%) Frame = +2 Query: 317 RDREQARPRQDPTETHQHPHR-----AREALQVQAGLPQARQGERKAVEG 451 + ++Q + +Q P + Q P + ++ Q Q PQ +Q E AV G Sbjct: 1506 QQQQQQQQQQQPQQQSQQPQQQQPQPQQQQQQQQQQQPQQQQKEYGAVSG 1555 >AB204559-1|BAD89804.1| 832|Apis mellifera soluble guanylyl cyclase beta-3 protein. Length = 832 Score = 23.4 bits (48), Expect = 5.3 Identities = 11/33 (33%), Positives = 17/33 (51%) Frame = -2 Query: 236 AIAPNVNDVSHFVHFHVRGKWNSSMCAESSGEQ 138 +++P V + S VH NSS +E S E+ Sbjct: 734 SLSPRVENRSAIVHSEASANANSSTSSEESREE 766 Database: bee Posted date: Oct 23, 2007 1:17 PM Number of letters in database: 146,343 Number of sequences in database: 438 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 325,485 Number of Sequences: 438 Number of extensions: 6366 Number of successful extensions: 11 Number of sequences better than 10.0: 2 Number of HSP's better than 10.0 without gapping: 9 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 11 length of database: 146,343 effective HSP length: 61 effective length of database: 119,625 effective search space used: 54309750 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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