Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030725E6_D05_e516_07.seq
(1548 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g57860.1 68414.m06565 60S ribosomal protein L21 similar to 60... 168 7e-42
At1g57660.1 68414.m06543 60S ribosomal protein L21 (RPL21E) simi... 168 7e-42
At1g09690.1 68414.m01088 60S ribosomal protein L21 (RPL21C) Simi... 167 1e-41
At1g09590.1 68414.m01076 60S ribosomal protein L21 (RPL21A) Simi... 167 1e-41
At5g47430.1 68418.m05844 expressed protein 30 3.6
At2g25760.2 68415.m03092 protein kinase family protein contains ... 29 6.2
At2g25760.1 68415.m03091 protein kinase family protein contains ... 29 6.2
>At1g57860.1 68414.m06565 60S ribosomal protein L21 similar to 60S
ribosomal protein L21 GI:3885884 from [Oryza sativa]
Length = 164
Score = 168 bits (409), Expect = 7e-42
Identities = 76/145 (52%), Positives = 105/145 (72%)
Frame = +3
Query: 138 LFARRFRTHGTIPLSTYMKVYKVGDIVDIRGNGAVQKGMPHKVYHGKTGRVYNVTAHALG 317
LFAR FR G IPLSTY++ +KVGD VD++ NGA+ KGMPHK YHG+TGR++NVT A+G
Sbjct: 14 LFARPFRKKGYIPLSTYLRTFKVGDYVDVKVNGAIHKGMPHKFYHGRTGRIWNVTKRAVG 73
Query: 318 VIVNKRVRGRILPKRINIRIEHVKHSKCRQDFLKRVKENERLLKEAKAAGKVVNLKRQPA 497
V VNK++ RI+ KRI++R+EHV+ S+C ++F R K+N+ L +AKA G+ ++ KRQP
Sbjct: 74 VEVNKQIGNRIIRKRIHVRVEHVQQSRCAEEFKLRKKQNDVLKADAKARGETISTKRQPK 133
Query: 498 PPKAAHIVSGAEKPVLLAPIPYEFV 572
PK +V G + PIPY+ V
Sbjct: 134 GPKPGFMVEGMTLET-VTPIPYDVV 157
>At1g57660.1 68414.m06543 60S ribosomal protein L21 (RPL21E) similar
to 60S ribosomal protein L21 GB:Q43291 GI:2851508 from
[Arabidopsis thaliana]
Length = 164
Score = 168 bits (409), Expect = 7e-42
Identities = 76/145 (52%), Positives = 105/145 (72%)
Frame = +3
Query: 138 LFARRFRTHGTIPLSTYMKVYKVGDIVDIRGNGAVQKGMPHKVYHGKTGRVYNVTAHALG 317
LFAR FR G IPLSTY++ +KVGD VD++ NGA+ KGMPHK YHG+TGR++NVT A+G
Sbjct: 14 LFARPFRKKGYIPLSTYLRTFKVGDYVDVKVNGAIHKGMPHKFYHGRTGRIWNVTKRAVG 73
Query: 318 VIVNKRVRGRILPKRINIRIEHVKHSKCRQDFLKRVKENERLLKEAKAAGKVVNLKRQPA 497
V VNK++ RI+ KRI++R+EHV+ S+C ++F R K+N+ L +AKA G+ ++ KRQP
Sbjct: 74 VEVNKQIGNRIIRKRIHVRVEHVQQSRCAEEFKLRKKQNDVLKADAKARGETISTKRQPK 133
Query: 498 PPKAAHIVSGAEKPVLLAPIPYEFV 572
PK +V G + PIPY+ V
Sbjct: 134 GPKPGFMVEGMTLET-VTPIPYDVV 157
>At1g09690.1 68414.m01088 60S ribosomal protein L21 (RPL21C) Similar
to ribosomal protein L21 (gb|L38826). ESTs
gb|AA395597,gb|ATTS5197 come from this gene
Length = 164
Score = 167 bits (407), Expect = 1e-41
Identities = 76/145 (52%), Positives = 104/145 (71%)
Frame = +3
Query: 138 LFARRFRTHGTIPLSTYMKVYKVGDIVDIRGNGAVQKGMPHKVYHGKTGRVYNVTAHALG 317
LFAR FR G IPLSTY++ +KVGD VD++ NGA+ KGMPHK YHG+TGR++NVT A+G
Sbjct: 14 LFARPFRKKGYIPLSTYLRTFKVGDYVDVKVNGAIHKGMPHKFYHGRTGRIWNVTKRAVG 73
Query: 318 VIVNKRVRGRILPKRINIRIEHVKHSKCRQDFLKRVKENERLLKEAKAAGKVVNLKRQPA 497
V VNK++ RI+ KRI++R+EHV+ S+C ++F R K+N+ L AKA G+ ++ KRQP
Sbjct: 74 VEVNKQIGNRIIRKRIHVRVEHVQQSRCAEEFKLRKKKNDELKAAAKANGETISTKRQPK 133
Query: 498 PPKAAHIVSGAEKPVLLAPIPYEFV 572
PK +V G + PIPY+ V
Sbjct: 134 GPKPGFMVEGMTLET-VTPIPYDVV 157
>At1g09590.1 68414.m01076 60S ribosomal protein L21 (RPL21A) Similar
to L21 family of ribosomal protein; amino acid sequence
is identical to F21M12.8
Length = 164
Score = 167 bits (407), Expect = 1e-41
Identities = 76/145 (52%), Positives = 104/145 (71%)
Frame = +3
Query: 138 LFARRFRTHGTIPLSTYMKVYKVGDIVDIRGNGAVQKGMPHKVYHGKTGRVYNVTAHALG 317
LFAR FR G IPLSTY++ +KVGD VD++ NGA+ KGMPHK YHG+TGR++NVT A+G
Sbjct: 14 LFARPFRKKGYIPLSTYLRTFKVGDYVDVKVNGAIHKGMPHKFYHGRTGRIWNVTKRAVG 73
Query: 318 VIVNKRVRGRILPKRINIRIEHVKHSKCRQDFLKRVKENERLLKEAKAAGKVVNLKRQPA 497
V VNK++ RI+ KRI++R+EHV+ S+C ++F R K+N+ L AKA G+ ++ KRQP
Sbjct: 74 VEVNKQIGNRIIRKRIHVRVEHVQQSRCAEEFKLRKKKNDELKAAAKANGETISTKRQPK 133
Query: 498 PPKAAHIVSGAEKPVLLAPIPYEFV 572
PK +V G + PIPY+ V
Sbjct: 134 GPKPGFMVEGMTLET-VTPIPYDVV 157
>At5g47430.1 68418.m05844 expressed protein
Length = 879
Score = 30.3 bits (65), Expect = 3.6
Identities = 15/32 (46%), Positives = 19/32 (59%), Gaps = 3/32 (9%)
Frame = +2
Query: 299 DRTRSRRDREQARP---RQDPTETHQHPHRAR 385
DRTR RRD +++R + TE QH HR R
Sbjct: 720 DRTRDRRDEDRSRDHRHHRGETERSQHHHRKR 751
>At2g25760.2 68415.m03092 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 676
Score = 29.5 bits (63), Expect = 6.2
Identities = 12/34 (35%), Positives = 20/34 (58%)
Frame = +2
Query: 302 RTRSRRDREQARPRQDPTETHQHPHRAREALQVQ 403
R+ +RRDR++ P+Q+P Q P R Q++
Sbjct: 5 RSNARRDRDKKNPKQNPIALKQSPVRRNPRRQLK 38
>At2g25760.1 68415.m03091 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 673
Score = 29.5 bits (63), Expect = 6.2
Identities = 12/34 (35%), Positives = 20/34 (58%)
Frame = +2
Query: 302 RTRSRRDREQARPRQDPTETHQHPHRAREALQVQ 403
R+ +RRDR++ P+Q+P Q P R Q++
Sbjct: 5 RSNARRDRDKKNPKQNPIALKQSPVRRNPRRQLK 38
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 25,245,593
Number of Sequences: 28952
Number of extensions: 469918
Number of successful extensions: 1158
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 1123
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1158
length of database: 12,070,560
effective HSP length: 84
effective length of database: 9,638,592
effective search space used: 4154233152
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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