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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 030725E6_C11_e563_05.seq
         (1569 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g76700.1 68414.m08925 DNAJ heat shock N-terminal domain-conta...    32   0.90 
At2g25050.1 68415.m02996 formin homology 2 domain-containing pro...    31   2.1  
At5g13300.1 68418.m01528 ARF GTPase-activating domain-containing...    29   8.4  

>At1g76700.1 68414.m08925 DNAJ heat shock N-terminal
           domain-containing protein similar to SP|P39101 CAJ1
           protein, Saccharomyces cerevisiae; contains Pfam profile
           PF00226 DnaJ domain
          Length = 398

 Score = 32.3 bits (70), Expect = 0.90
 Identities = 23/74 (31%), Positives = 31/74 (41%), Gaps = 1/74 (1%)
 Frame = +2

Query: 518 IAYITPNYASKSSENRKIFFNRYIITSWFPRLRLHFICS-LAASEKVHITFHSYPNLKEQ 694
           I YI    A+K    + I+     I  WF R + HFI S L A+   +  F     +K Q
Sbjct: 182 IGYIYVRQAAKELGKKAIYLGVPFIAEWF-RNKGHFIKSQLTAATGAYALFQLQEEMKRQ 240

Query: 695 MNEEKKSLSXSLAE 736
           +N E       L E
Sbjct: 241 LNTEGNYTEEELEE 254


>At2g25050.1 68415.m02996 formin homology 2 domain-containing protein
            / FH2 domain-containing protein contains formin homology
            2 domain, Pfam:PF02128
          Length = 1111

 Score = 31.1 bits (67), Expect = 2.1
 Identities = 16/58 (27%), Positives = 21/58 (36%)
 Frame = +3

Query: 1263 GXPAXVXXAXRGPVPXEXXXXXLTFPXXXPRXPXXVGXRXXPXPAXTSXXXRXRXPPP 1436
            G P+        P+P       L+ P   P  P     R  P P+ TS     + PPP
Sbjct: 546  GPPSAEAAVTSSPLPPLKPLRILSRPPPPPPPPPISSLRSTPSPSSTSNSIATQGPPP 603


>At5g13300.1 68418.m01528 ARF GTPase-activating domain-containing
           protein similar to GCN4-complementing protein (GCP1)
           GI:6465806 from [Arabidopsis thaliana]
          Length = 768

 Score = 29.1 bits (62), Expect = 8.4
 Identities = 12/48 (25%), Positives = 25/48 (52%)
 Frame = +2

Query: 608 RLRLHFICSLAASEKVHITFHSYPNLKEQMNEEKKSLSXSLAEKMYIR 751
           R  +HF   L  S+K  +     P+  + ++ ++K +    AEK+++R
Sbjct: 531 RSAIHFDPGLTVSDKSRVMVTGKPSYADMISIKEKYIQAKYAEKLFVR 578


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 23,944,503
Number of Sequences: 28952
Number of extensions: 423019
Number of successful extensions: 957
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 694
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 930
length of database: 12,070,560
effective HSP length: 84
effective length of database: 9,638,592
effective search space used: 4221703296
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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