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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 030725E6_C06_e523_06.seq
         (1550 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC1442.06 |||20S proteasome component alpha 2|Schizosaccharomy...    56   1e-08
SPAC13C5.01c ||SPAC31A2.17c|20S proteasome component alpha 3|Sch...    37   0.009
SPBC106.16 |||20S proteasome component alpha 4|Schizosaccharomyc...    37   0.009
SPAC323.02c |||20S proteasome component alpha 5|Schizosaccharomy...    36   0.015
SPBC646.16 |||20S proteasome component alpha 1|Schizosaccharomyc...    30   0.99 

>SPCC1442.06 |||20S proteasome component alpha 2|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 245

 Score = 56.4 bits (130), Expect = 1e-08
 Identities = 31/55 (56%), Positives = 40/55 (72%)
 Frame = +3

Query: 36  SERYSFSLTTFSPSGKLVSXPSML*GLXADGGTSVGIXASNGVVIATENKHKSIL 200
           +++Y+FSLTTFSP+GKLV     L  + A G TSVGI A++GVV+ATE K  S L
Sbjct: 2   TDKYAFSLTTFSPNGKLVQIEYALNAVNA-GVTSVGIKATDGVVLATEKKPTSEL 55



 Score = 53.2 bits (122), Expect = 9e-08
 Identities = 27/54 (50%), Positives = 36/54 (66%), Gaps = 1/54 (1%)
 Frame = +2

Query: 254 MVYSGMXPDYRXLVXQAXKMXQ-QYYLLXHEPIPXXXMVQRVATVMXEYTQSGG 412
           MVYSGM PD+R LV ++ K+ Q  Y  + +E  P   +VQ +A+VM E TQSGG
Sbjct: 73  MVYSGMGPDFRVLVDKSRKVAQTTYKKIYNEYPPTKILVQEIASVMQESTQSGG 126


>SPAC13C5.01c ||SPAC31A2.17c|20S proteasome component alpha
           3|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 248

 Score = 36.7 bits (81), Expect = 0.009
 Identities = 17/50 (34%), Positives = 27/50 (54%)
 Frame = +2

Query: 263 SGMXPDYRXLVXQAXKMXQQYYLLXHEPIPXXXMVQRVATVMXEYTQSGG 412
           +G+  D   L+  A ++ QQY    +E +P   +V+RV  +   YTQ GG
Sbjct: 77  AGLTADANILINYARRVGQQYLQTFNEEMPCEQLVRRVCDLKQGYTQYGG 126


>SPBC106.16 |||20S proteasome component alpha 4|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 259

 Score = 36.7 bits (81), Expect = 0.009
 Identities = 17/53 (32%), Positives = 29/53 (54%)
 Frame = +2

Query: 254 MVYSGMXPDYRXLVXQAXKMXQQYYLLXHEPIPXXXMVQRVATVMXEYTQSGG 412
           + ++G+  D R L+ +A    Q + L   +P+    + + VA V  +YTQSGG
Sbjct: 72  LAFAGLNADARILIDKARVEAQNHKLNLADPVSIEYLTRYVAGVQQKYTQSGG 124


>SPAC323.02c |||20S proteasome component alpha 5|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 247

 Score = 35.9 bits (79), Expect = 0.015
 Identities = 18/58 (31%), Positives = 29/58 (50%)
 Frame = +3

Query: 45  YSFSLTTFSPSGKLVSXPSML*GLXADGGTSVGIXASNGVVIATENKHKSILYDEHSV 218
           Y   + TFSP G+L      +  +   G T++G+   + VV+  E +  S L + HSV
Sbjct: 8   YDRGVNTFSPEGRLFQVEYAIEAIKL-GSTAIGVKTKDAVVLGVEKRLTSPLMESHSV 64


>SPBC646.16 |||20S proteasome component alpha 1|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 244

 Score = 29.9 bits (64), Expect = 0.99
 Identities = 19/63 (30%), Positives = 28/63 (44%)
 Frame = +3

Query: 30  RGSERYSFSLTTFSPSGKLVSXPSML*GLXADGGTSVGIXASNGVVIATENKHKSILYDE 209
           RG +R   ++T FSP G+L             G TSVG+   N   + ++ K    L D 
Sbjct: 5   RGFDR---TITVFSPEGRLYQVEYAFKAFNNAGITSVGVTGKNCACVISQKKVPDKLIDA 61

Query: 210 HSV 218
            +V
Sbjct: 62  STV 64


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,751,459
Number of Sequences: 5004
Number of extensions: 21164
Number of successful extensions: 36
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 33
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 35
length of database: 2,362,478
effective HSP length: 76
effective length of database: 1,982,174
effective search space used: 872156560
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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