BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= 030725E6_C06_e523_06.seq (1550 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC1442.06 |||20S proteasome component alpha 2|Schizosaccharomy... 56 1e-08 SPAC13C5.01c ||SPAC31A2.17c|20S proteasome component alpha 3|Sch... 37 0.009 SPBC106.16 |||20S proteasome component alpha 4|Schizosaccharomyc... 37 0.009 SPAC323.02c |||20S proteasome component alpha 5|Schizosaccharomy... 36 0.015 SPBC646.16 |||20S proteasome component alpha 1|Schizosaccharomyc... 30 0.99 >SPCC1442.06 |||20S proteasome component alpha 2|Schizosaccharomyces pombe|chr 3|||Manual Length = 245 Score = 56.4 bits (130), Expect = 1e-08 Identities = 31/55 (56%), Positives = 40/55 (72%) Frame = +3 Query: 36 SERYSFSLTTFSPSGKLVSXPSML*GLXADGGTSVGIXASNGVVIATENKHKSIL 200 +++Y+FSLTTFSP+GKLV L + A G TSVGI A++GVV+ATE K S L Sbjct: 2 TDKYAFSLTTFSPNGKLVQIEYALNAVNA-GVTSVGIKATDGVVLATEKKPTSEL 55 Score = 53.2 bits (122), Expect = 9e-08 Identities = 27/54 (50%), Positives = 36/54 (66%), Gaps = 1/54 (1%) Frame = +2 Query: 254 MVYSGMXPDYRXLVXQAXKMXQ-QYYLLXHEPIPXXXMVQRVATVMXEYTQSGG 412 MVYSGM PD+R LV ++ K+ Q Y + +E P +VQ +A+VM E TQSGG Sbjct: 73 MVYSGMGPDFRVLVDKSRKVAQTTYKKIYNEYPPTKILVQEIASVMQESTQSGG 126 >SPAC13C5.01c ||SPAC31A2.17c|20S proteasome component alpha 3|Schizosaccharomyces pombe|chr 1|||Manual Length = 248 Score = 36.7 bits (81), Expect = 0.009 Identities = 17/50 (34%), Positives = 27/50 (54%) Frame = +2 Query: 263 SGMXPDYRXLVXQAXKMXQQYYLLXHEPIPXXXMVQRVATVMXEYTQSGG 412 +G+ D L+ A ++ QQY +E +P +V+RV + YTQ GG Sbjct: 77 AGLTADANILINYARRVGQQYLQTFNEEMPCEQLVRRVCDLKQGYTQYGG 126 >SPBC106.16 |||20S proteasome component alpha 4|Schizosaccharomyces pombe|chr 2|||Manual Length = 259 Score = 36.7 bits (81), Expect = 0.009 Identities = 17/53 (32%), Positives = 29/53 (54%) Frame = +2 Query: 254 MVYSGMXPDYRXLVXQAXKMXQQYYLLXHEPIPXXXMVQRVATVMXEYTQSGG 412 + ++G+ D R L+ +A Q + L +P+ + + VA V +YTQSGG Sbjct: 72 LAFAGLNADARILIDKARVEAQNHKLNLADPVSIEYLTRYVAGVQQKYTQSGG 124 >SPAC323.02c |||20S proteasome component alpha 5|Schizosaccharomyces pombe|chr 1|||Manual Length = 247 Score = 35.9 bits (79), Expect = 0.015 Identities = 18/58 (31%), Positives = 29/58 (50%) Frame = +3 Query: 45 YSFSLTTFSPSGKLVSXPSML*GLXADGGTSVGIXASNGVVIATENKHKSILYDEHSV 218 Y + TFSP G+L + + G T++G+ + VV+ E + S L + HSV Sbjct: 8 YDRGVNTFSPEGRLFQVEYAIEAIKL-GSTAIGVKTKDAVVLGVEKRLTSPLMESHSV 64 >SPBC646.16 |||20S proteasome component alpha 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 244 Score = 29.9 bits (64), Expect = 0.99 Identities = 19/63 (30%), Positives = 28/63 (44%) Frame = +3 Query: 30 RGSERYSFSLTTFSPSGKLVSXPSML*GLXADGGTSVGIXASNGVVIATENKHKSILYDE 209 RG +R ++T FSP G+L G TSVG+ N + ++ K L D Sbjct: 5 RGFDR---TITVFSPEGRLYQVEYAFKAFNNAGITSVGVTGKNCACVISQKKVPDKLIDA 61 Query: 210 HSV 218 +V Sbjct: 62 STV 64 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,751,459 Number of Sequences: 5004 Number of extensions: 21164 Number of successful extensions: 36 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 33 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 35 length of database: 2,362,478 effective HSP length: 76 effective length of database: 1,982,174 effective search space used: 872156560 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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