Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030725E6_C06_e523_06.seq
(1550 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g79210.1 68414.m09235 20S proteasome alpha subunit B, putativ... 71 2e-12
At1g16470.1 68414.m01970 20S proteasome alpha subunit B (PAB1) (... 71 2e-12
At2g05840.1 68415.m00632 20S proteasome alpha subunit A2 (PAA2) ... 38 0.024
At5g35590.1 68418.m04237 20S proteasome alpha subunit A1 (PAA1) ... 36 0.072
At3g14290.1 68416.m01808 20S proteasome alpha subunit E2 (PAE2) ... 36 0.095
At1g53850.1 68414.m06129 20S proteasome alpha subunit E1 (PAE1) ... 36 0.095
At3g22110.1 68416.m02791 20S proteasome alpha subunit C (PAC1) (... 35 0.13
At5g66140.1 68418.m08332 20S proteasome alpha subunit D2 (PAD2) ... 34 0.22
At3g51260.1 68416.m05611 20S proteasome alpha subunit D (PAD1) 34 0.22
At2g27020.1 68415.m03244 20S proteasome alpha subunit G (PAG1) (... 32 1.2
At5g42790.1 68418.m05212 20S proteasome alpha subunit F1 (PAF1) ... 30 3.6
At1g47250.1 68414.m05231 20S proteasome alpha subunit F2 (PAF2) ... 30 3.6
>At1g79210.1 68414.m09235 20S proteasome alpha subunit B, putative
nearly identical to SP|O23708 Proteasome subunit alpha
type 2 (EC 3.4.25.1) (20S proteasome alpha subunit B)
{Arabidopsis thaliana} and to At1g16470
Length = 235
Score = 70.9 bits (166), Expect = 2e-12
Identities = 40/62 (64%), Positives = 44/62 (70%)
Frame = +3
Query: 33 GSERYSFSLTTFSPSGKLVSXPSML*GLXADGGTSVGIXASNGVVIATENKHKSILYDEH 212
G +YSFSLTTFSPSGKLV L + + G TS+GI ASNGVVIATE K SIL DE
Sbjct: 2 GDSQYSFSLTTFSPSGKLVQIEHALTAVGS-GQTSLGIKASNGVVIATEKKLPSILVDEA 60
Query: 213 SV 218
SV
Sbjct: 61 SV 62
Score = 64.5 bits (150), Expect = 2e-10
Identities = 29/52 (55%), Positives = 36/52 (69%)
Frame = +2
Query: 257 VYSGMXPDYRXLVXQAXKMXQQYYLLXHEPIPXXXMVQRVATVMXEYTQSGG 412
VYSGM PD+R LV ++ K +QY L EPIP +V+ ATVM E+TQSGG
Sbjct: 75 VYSGMGPDFRVLVRKSRKQAEQYLRLYKEPIPVTQLVRETATVMQEFTQSGG 126
>At1g16470.1 68414.m01970 20S proteasome alpha subunit B (PAB1)
(PRC3) identical to proteasome subunit alpha type 2
SP:O23708, GI:6093778; identical to cDNA proteasome
subunit prc3 GI:2511573
Length = 235
Score = 70.9 bits (166), Expect = 2e-12
Identities = 40/62 (64%), Positives = 44/62 (70%)
Frame = +3
Query: 33 GSERYSFSLTTFSPSGKLVSXPSML*GLXADGGTSVGIXASNGVVIATENKHKSILYDEH 212
G +YSFSLTTFSPSGKLV L + + G TS+GI ASNGVVIATE K SIL DE
Sbjct: 2 GDSQYSFSLTTFSPSGKLVQIEHALTAVGS-GQTSLGIKASNGVVIATEKKLPSILVDEA 60
Query: 213 SV 218
SV
Sbjct: 61 SV 62
Score = 64.9 bits (151), Expect = 1e-10
Identities = 29/53 (54%), Positives = 37/53 (69%)
Frame = +2
Query: 254 MVYSGMXPDYRXLVXQAXKMXQQYYLLXHEPIPXXXMVQRVATVMXEYTQSGG 412
+VYSGM PD+R LV ++ K +QY L EPIP +V+ ATVM E+TQSGG
Sbjct: 74 VVYSGMGPDFRVLVRKSRKQAEQYLRLYKEPIPVTQLVRETATVMQEFTQSGG 126
>At2g05840.1 68415.m00632 20S proteasome alpha subunit A2 (PAA2)
identical to GB:AF043519
Length = 246
Score = 37.5 bits (83), Expect = 0.024
Identities = 22/64 (34%), Positives = 31/64 (48%), Gaps = 1/64 (1%)
Frame = +3
Query: 30 RGSER-YSFSLTTFSPSGKLVSXPSML*GLXADGGTSVGIXASNGVVIATENKHKSILYD 206
RGS Y +T FSP G+L + A G TS+G+ + V + T+ K L D
Sbjct: 3 RGSGAGYDRHITIFSPEGRLFQVEYAFKAVKAAGITSIGVRGKDSVCVVTQKKVPDKLLD 62
Query: 207 EHSV 218
+ SV
Sbjct: 63 QSSV 66
>At5g35590.1 68418.m04237 20S proteasome alpha subunit A1 (PAA1)
(PRC1) identical to proteasome subunit alpha type 6-1
SP:O81146 GI:12643647 from [Arabidopsis thaliana];
identical to cDNA proteasome subunit prc1 GI:2511587
Length = 246
Score = 35.9 bits (79), Expect = 0.072
Identities = 21/64 (32%), Positives = 30/64 (46%), Gaps = 1/64 (1%)
Frame = +3
Query: 30 RGSER-YSFSLTTFSPSGKLVSXPSML*GLXADGGTSVGIXASNGVVIATENKHKSILYD 206
RGS Y +T FSP G+L + G TS+G+ + V + T+ K L D
Sbjct: 3 RGSGAGYDRHITIFSPEGRLFQVEYAFKAVKTAGITSIGVRGKDSVCVVTQKKVPDKLLD 62
Query: 207 EHSV 218
+ SV
Sbjct: 63 QSSV 66
>At3g14290.1 68416.m01808 20S proteasome alpha subunit E2 (PAE2)
identical to 20S proteasome subunit PAE2 GB:AAC32061
from [Arabidopsis thaliana]
Length = 237
Score = 35.5 bits (78), Expect = 0.095
Identities = 19/58 (32%), Positives = 29/58 (50%)
Frame = +3
Query: 45 YSFSLTTFSPSGKLVSXPSML*GLXADGGTSVGIXASNGVVIATENKHKSILYDEHSV 218
Y + TFSP G+L + + G T++G+ GVV+A E + S L + SV
Sbjct: 8 YDRGVNTFSPEGRLFQVEYAIEAIKL-GSTAIGVKTKEGVVLAVEKRITSPLLEPSSV 64
>At1g53850.1 68414.m06129 20S proteasome alpha subunit E1 (PAE1)
identical to 20S proteasome subunit PAE1 GI:3421087 from
[Arabidopsis thaliana]
Length = 237
Score = 35.5 bits (78), Expect = 0.095
Identities = 19/58 (32%), Positives = 29/58 (50%)
Frame = +3
Query: 45 YSFSLTTFSPSGKLVSXPSML*GLXADGGTSVGIXASNGVVIATENKHKSILYDEHSV 218
Y + TFSP G+L + + G T++G+ GVV+A E + S L + SV
Sbjct: 8 YDRGVNTFSPEGRLFQVEYAIEAIKL-GSTAIGVKTKEGVVLAVEKRITSPLLEPSSV 64
>At3g22110.1 68416.m02791 20S proteasome alpha subunit C (PAC1)
(PRC9) identical to GB:AAC32057 from [Arabidopsis
thaliana] (Genetics (1998) 149 (2), 677-692); identical
to cDNA proteasome subunit prc9 GI:2511583
Length = 250
Score = 35.1 bits (77), Expect = 0.13
Identities = 16/50 (32%), Positives = 24/50 (48%)
Frame = +2
Query: 263 SGMXPDYRXLVXQAXKMXQQYYLLXHEPIPXXXMVQRVATVMXEYTQSGG 412
+G+ D L+ A Q+Y + EP+P +VQ + YTQ GG
Sbjct: 77 AGIMSDANILINTARVQAQRYTFMYQEPMPVEQLVQSLCDTKQGYTQFGG 126
Score = 34.3 bits (75), Expect = 0.22
Identities = 19/55 (34%), Positives = 29/55 (52%)
Frame = +3
Query: 36 SERYSFSLTTFSPSGKLVSXPSML*GLXADGGTSVGIXASNGVVIATENKHKSIL 200
S RY T FSP G+L + + + G+++GI + +GVV+ E K S L
Sbjct: 2 SRRYDSRTTIFSPEGRLYQVEYAMEAI-GNAGSAIGILSKDGVVLIGEKKVTSKL 55
>At5g66140.1 68418.m08332 20S proteasome alpha subunit D2 (PAD2)
(PRS1) (PRC6) identical to SP|O24616 Proteasome subunit
alpha type 7-2 (EC 3.4.25.1) (20S proteasome alpha
subunit D2) {Arabidopsis thaliana}
Length = 250
Score = 34.3 bits (75), Expect = 0.22
Identities = 20/58 (34%), Positives = 28/58 (48%)
Frame = +3
Query: 42 RYSFSLTTFSPSGKLVSXPSML*GLXADGGTSVGIXASNGVVIATENKHKSILYDEHS 215
RY ++T FSP G L L + G +VG+ ++ VV+A E K L D S
Sbjct: 3 RYDRAITVFSPDGHLFQVEYALEAV-RKGNAAVGVRGTDTVVLAVEKKSTPKLQDSRS 59
Score = 33.1 bits (72), Expect = 0.51
Identities = 15/50 (30%), Positives = 27/50 (54%)
Frame = +2
Query: 263 SGMXPDYRXLVXQAXKMXQQYYLLXHEPIPXXXMVQRVATVMXEYTQSGG 412
+G+ D R L+ +A Q + L +P+ + + +A + +YTQSGG
Sbjct: 75 AGLKADARVLINKARIECQSHRLTLEDPVTVEYITRYIAGLQQKYTQSGG 124
>At3g51260.1 68416.m05611 20S proteasome alpha subunit D (PAD1)
Length = 250
Score = 34.3 bits (75), Expect = 0.22
Identities = 20/58 (34%), Positives = 28/58 (48%)
Frame = +3
Query: 42 RYSFSLTTFSPSGKLVSXPSML*GLXADGGTSVGIXASNGVVIATENKHKSILYDEHS 215
RY ++T FSP G L L + G +VG+ ++ VV+A E K L D S
Sbjct: 3 RYDRAITVFSPDGHLFQVEYALEAV-RKGNAAVGVRGTDTVVLAVEKKSTPKLQDSRS 59
Score = 33.1 bits (72), Expect = 0.51
Identities = 15/50 (30%), Positives = 27/50 (54%)
Frame = +2
Query: 263 SGMXPDYRXLVXQAXKMXQQYYLLXHEPIPXXXMVQRVATVMXEYTQSGG 412
+G+ D R L+ +A Q + L +P+ + + +A + +YTQSGG
Sbjct: 75 AGLKADARVLINKARIECQSHRLTLEDPVTVEYITRYIAGLQQKYTQSGG 124
>At2g27020.1 68415.m03244 20S proteasome alpha subunit G (PAG1)
(PRC8) identical to proteasome subunit alpha type 3
SP:O23715, GI:12644056 from [Arabidopsis thaliana];
identical to cDNA proteasome subunit prc8 GI:2511591
Length = 249
Score = 31.9 bits (69), Expect = 1.2
Identities = 16/45 (35%), Positives = 24/45 (53%)
Frame = +3
Query: 45 YSFSLTTFSPSGKLVSXPSML*GLXADGGTSVGIXASNGVVIATE 179
Y S+TTFSP G++ + + GT VGI +G+V+ E
Sbjct: 8 YDLSVTTFSPDGRVFQIEYAAKAVD-NSGTVVGIKCKDGIVMGVE 51
>At5g42790.1 68418.m05212 20S proteasome alpha subunit F1 (PAF1)
(gb|AAC32062.1)
Length = 278
Score = 30.3 bits (65), Expect = 3.6
Identities = 16/53 (30%), Positives = 30/53 (56%)
Frame = +3
Query: 42 RYSFSLTTFSPSGKLVSXPSML*GLXADGGTSVGIXASNGVVIATENKHKSIL 200
+Y +TT+SP+G+L + + G ++G+ + + VV+A NK +S L
Sbjct: 5 QYDTDVTTWSPTGRLFQVEYAMEAV-KQGSAAIGLRSRSHVVLACVNKAQSEL 56
>At1g47250.1 68414.m05231 20S proteasome alpha subunit F2 (PAF2)
(PRC2B) (PRS1) identical to GB:AAC32063 from
[Arabidopsis thaliana] (Genetics 149 (2), 677-692
(1998)); identical to cDNA proteasome subunit prc2b
GI:2511585
Length = 277
Score = 30.3 bits (65), Expect = 3.6
Identities = 16/53 (30%), Positives = 30/53 (56%)
Frame = +3
Query: 42 RYSFSLTTFSPSGKLVSXPSML*GLXADGGTSVGIXASNGVVIATENKHKSIL 200
+Y +TT+SP+G+L + + G ++G+ + + VV+A NK +S L
Sbjct: 5 QYDTDVTTWSPTGRLFQVEYAMEAV-KQGSAAIGLRSRSHVVLACVNKAQSEL 56
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,277,999
Number of Sequences: 28952
Number of extensions: 114319
Number of successful extensions: 197
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 185
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 193
length of database: 12,070,560
effective HSP length: 84
effective length of database: 9,638,592
effective search space used: 4163871744
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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