Samia BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 030725E6_C03_e499_05.seq
         (1551 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC3B9.12 |||TRAPP complex subunit Trs23 |Schizosaccharomyces p...   111   3e-25
SPAC3G9.16c |bet5|SPAC688.15|TRAPP complex subunit Bet5 |Schizos...    42   2e-04
SPBC11G11.04 |trs20||TRAPP complex subunit Trs20 |Schizosaccharo...    33   0.080
SPBC16G5.03 |||ubiquitin-protein ligase E3 |Schizosaccharomyces ...    27   5.3  
SPBC1861.05 |||carbohydrate kinase|Schizosaccharomyces pombe|chr...    27   7.0  
SPAC18B11.03c |||N-acetyltransferase |Schizosaccharomyces pombe|...    27   7.0  
SPBC14F5.03c |kap123||karyopherin Kap123|Schizosaccharomyces pom...    27   9.2  

>SPBC3B9.12 |||TRAPP complex subunit Trs23 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 132

 Score =  111 bits (266), Expect = 3e-25
 Identities = 49/104 (47%), Positives = 72/104 (69%)
 Frame = +2

Query: 437 TTNEKIVLASMFYPLFALACQLSPVPKSSGIETLTSDTFKLSCFQTLTGVKFIIVTDPNM 616
           T NE +VLA   + + A++ Q+SP+P SSGI+ L + TF +   QT TG+KF++ T+   
Sbjct: 27  TPNEYLVLAGTIHGVHAISTQISPLPGSSGIQLLEAGTFNMHILQTHTGMKFVLFTEKKT 86

Query: 617 QGAEVVLKRIYELYSDYALKSPFYSLEMPIRCELFDTSLHTLLE 748
             A + L++ YELYSDY LK+PFY+LEMPI+C+LFD  L   ++
Sbjct: 87  TNARLQLQKFYELYSDYVLKNPFYTLEMPIKCQLFDEQLKRYID 130


>SPAC3G9.16c |bet5|SPAC688.15|TRAPP complex subunit Bet5
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 155

 Score = 42.3 bits (95), Expect = 2e-04
 Identities = 23/72 (31%), Positives = 40/72 (55%), Gaps = 7/72 (9%)
 Frame = +2

Query: 539 TSDTFKLSCFQTLTGVKFIIVTDPNMQGAEVVLKRIY-ELYSDYALKSPFYSLEMP---- 703
           T+  +KL  ++T T ++ I +T+P +     VL++IY  LY ++ +K P Y+   P    
Sbjct: 74  TTSKYKLHFYETPTNLRLIFITNPKIDSLTHVLQQIYTTLYVEFVVKHPLYTHVPPSAEE 133

Query: 704 --IRCELFDTSL 733
             I CE+F  +L
Sbjct: 134 GGINCEIFRITL 145


>SPBC11G11.04 |trs20||TRAPP complex subunit Trs20
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 136

 Score = 33.5 bits (73), Expect = 0.080
 Identities = 16/63 (25%), Positives = 35/63 (55%), Gaps = 1/63 (1%)
 Frame = +2

Query: 557 LSCFQTLTGVKFIIV-TDPNMQGAEVVLKRIYELYSDYALKSPFYSLEMPIRCELFDTSL 733
           +S + T + ++F+++  + +    ++  + ++ELY    L SPFY    PIR + FD  +
Sbjct: 70  ISAYVTPSNMRFMLLHQNQSADNIKLFFQELHELYIK-TLMSPFYQPNQPIRSQAFDLKV 128

Query: 734 HTL 742
            ++
Sbjct: 129 RSI 131


>SPBC16G5.03 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 268

 Score = 27.5 bits (58), Expect = 5.3
 Identities = 14/43 (32%), Positives = 26/43 (60%)
 Frame = -3

Query: 760 LLDELEQRVQRRIEQLASYRHLERVERALEGVVRVQLIDPLQD 632
           LL EL++ +Q R   ++SY + +R     EG+  VQL+  +++
Sbjct: 214 LLHELQRYLQFRPLSISSYLYSKRYLYGPEGLTMVQLLSQIEN 256


>SPBC1861.05 |||carbohydrate kinase|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 747

 Score = 27.1 bits (57), Expect = 7.0
 Identities = 13/29 (44%), Positives = 17/29 (58%)
 Frame = +2

Query: 515 KSSGIETLTSDTFKLSCFQTLTGVKFIIV 601
           KS+GIE +T +     CF  L  +K IIV
Sbjct: 620 KSTGIEEITENGILQKCFHLLPFIKNIIV 648


>SPAC18B11.03c |||N-acetyltransferase |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 440

 Score = 27.1 bits (57), Expect = 7.0
 Identities = 9/30 (30%), Positives = 19/30 (63%)
 Frame = +2

Query: 107 LCKPYSTMVIYGVYIVSKSGGLIYNYDHNI 196
           LC    T+ ++ VY+++    L++ YDH++
Sbjct: 98  LCYDDETLPLWMVYVLNDKSELVFIYDHSL 127


>SPBC14F5.03c |kap123||karyopherin Kap123|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 1067

 Score = 26.6 bits (56), Expect = 9.2
 Identities = 14/46 (30%), Positives = 24/46 (52%)
 Frame = +2

Query: 386 EIKENYPLNLKFGRARATTNEKIVLASMFYPLFALACQLSPVPKSS 523
           +I E+ P  L   R+    +  +  + +FYP+F  AC  S  P++S
Sbjct: 313 DIDEDCPARLAL-RSIDLLSTHLSPSQVFYPMFEAACAFSQSPQAS 357


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 4,922,891
Number of Sequences: 5004
Number of extensions: 88626
Number of successful extensions: 179
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 176
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 179
length of database: 2,362,478
effective HSP length: 76
effective length of database: 1,982,174
effective search space used: 872156560
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)