Samia BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 030725E6_C01_e483_05.seq
         (1486 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At4g36020.1 68417.m05128 cold-shock DNA-binding family protein c...    69   1e-11
At2g21060.1 68415.m02500 cold-shock DNA-binding family protein /...    60   3e-09
At2g17870.1 68415.m02070 cold-shock DNA-binding family protein c...    59   6e-09
At4g38680.1 68417.m05477 cold-shock DNA-binding family protein c...    59   8e-09
At5g48920.1 68418.m06052 hydroxyproline-rich glycoprotein family...    31   2.6  

>At4g36020.1 68417.m05128 cold-shock DNA-binding family protein
           contains Pfam domains, PF00313: 'Cold-shock' DNA-binding
           domain and PF00098: Zinc knuckle
          Length = 299

 Score = 68.5 bits (160), Expect = 1e-11
 Identities = 35/80 (43%), Positives = 49/80 (61%), Gaps = 1/80 (1%)
 Frame = +1

Query: 268 AEKVSGTVKWFNVKSGYGFINRNDTKEDVFVHQTAIIRNNPRKAVRSVGDGEAVEFAVVA 447
           A + +G V WFN   GYGFI  +D   ++FVHQ++I+     +  RS+  G+AVEFA+  
Sbjct: 8   AARSTGKVNWFNASKGYGFITPDDGSVELFVHQSSIV----SEGYRSLTVGDAVEFAITQ 63

Query: 448 GEKG-YEAARVTGPGGESVK 504
           G  G  +A  VT PGG S+K
Sbjct: 64  GSDGKTKAVNVTAPGGGSLK 83


>At2g21060.1 68415.m02500 cold-shock DNA-binding family protein /
           glycine-rich protein (GRP2) identical to Glycine-rich
           protein 2b (AtGRP2b) [Arabidopsis thaliana]
           SWISS-PROT:Q38896; contains Pfam domains PF00313:
           'Cold-shock' DNA-binding domain and PF00098: Zinc
           knuckle
          Length = 201

 Score = 60.5 bits (140), Expect = 3e-09
 Identities = 32/81 (39%), Positives = 49/81 (60%), Gaps = 1/81 (1%)
 Frame = +1

Query: 271 EKVSGTVKWFNVKSGYGFINRNDTKEDVFVHQTAIIRNNPRKAVRSVGDGEAVEFAVVAG 450
           ++  GTVKWF+ + G+GFI  +D  +D+FVHQ++I      +  RS+   E+VEF V   
Sbjct: 13  DRRKGTVKWFDTQKGFGFITPSDGGDDLFVHQSSI----RSEGFRSLAAEESVEFDVEVD 68

Query: 451 EKGY-EAARVTGPGGESVKGS 510
             G  +A  V+GP G  V+G+
Sbjct: 69  NSGRPKAIEVSGPDGAPVQGN 89


>At2g17870.1 68415.m02070 cold-shock DNA-binding family protein
           contains Pfam domains, PF00313: 'Cold-shock' DNA-binding
           domain and PF00098: Zinc knuckle
          Length = 301

 Score = 59.3 bits (137), Expect = 6e-09
 Identities = 32/79 (40%), Positives = 47/79 (59%), Gaps = 1/79 (1%)
 Frame = +1

Query: 268 AEKVSGTVKWFNVKSGYGFINRNDTKEDVFVHQTAIIRNNPRKAVRSVGDGEAVEFAVVA 447
           A +  G V WF+   GYGFI  +D  E++FVHQ++I+ +      RS+  GE+VE+ +  
Sbjct: 8   AARSIGKVSWFSDGKGYGFITPDDGGEELFVHQSSIVSD----GFRSLTLGESVEYEIAL 63

Query: 448 GEKG-YEAARVTGPGGESV 501
           G  G  +A  VT PGG S+
Sbjct: 64  GSDGKTKAIEVTAPGGGSL 82


>At4g38680.1 68417.m05477 cold-shock DNA-binding family protein
           contains Pfam domains PF00313: 'Cold-shock' DNA-binding
           domain and PF00098: Zinc knuckle
          Length = 203

 Score = 58.8 bits (136), Expect = 8e-09
 Identities = 32/81 (39%), Positives = 49/81 (60%), Gaps = 1/81 (1%)
 Frame = +1

Query: 271 EKVSGTVKWFNVKSGYGFINRNDTKEDVFVHQTAIIRNNPRKAVRSVGDGEAVEFAV-VA 447
           E+  G+VKWF+ + G+GFI  +D  +D+FVHQ++I      +  RS+   EAVEF V + 
Sbjct: 9   ERRKGSVKWFDTQKGFGFITPDDGGDDLFVHQSSI----RSEGFRSLAAEEAVEFEVEID 64

Query: 448 GEKGYEAARVTGPGGESVKGS 510
                +A  V+GP G  V+G+
Sbjct: 65  NNNRPKAIDVSGPDGAPVQGN 85


>At5g48920.1 68418.m06052 hydroxyproline-rich glycoprotein family
           protein contains proline-rich extensin domains,
           INTERPRO:IPR002965
          Length = 205

 Score = 30.7 bits (66), Expect = 2.6
 Identities = 14/43 (32%), Positives = 25/43 (58%), Gaps = 3/43 (6%)
 Frame = +2

Query: 557 HVKVGDVVVKEPHAE---VVSGAVDLHPIKGVHKGMRVRNRAH 676
           H+KV + +V+ PH E   VV    D+H ++ +HK  ++   +H
Sbjct: 140 HLKVQETIVQGPHGEQTRVVMLEEDIHLVEDIHKTEKLSRPSH 182


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,358,303
Number of Sequences: 28952
Number of extensions: 291984
Number of successful extensions: 858
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 841
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 857
length of database: 12,070,560
effective HSP length: 84
effective length of database: 9,638,592
effective search space used: 3951822720
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)