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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 030725E6_A08_e537_02.seq
         (1482 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBP8B7.20c |||RNA methyltransferase Nop2 |Schizosaccharomyces p...   396   e-111
SPAC23C4.17 |||tRNA |Schizosaccharomyces pombe|chr 1|||Manual          81   3e-16
SPAC17D4.04 ||SPAC458.01|tRNA |Schizosaccharomyces pombe|chr 1||...    79   1e-15
SPAC2C4.06c |||rRNA methyltransferase |Schizosaccharomyces pombe...    68   4e-12
SPAC13F5.03c |||glycerol dehydrogenase |Schizosaccharomyces pomb...    28   3.8  
SPBC337.16 |cho1||phosphatidyl-N-methylethanolamine N-methyltran...    27   8.7  

>SPBP8B7.20c |||RNA methyltransferase Nop2 |Schizosaccharomyces
            pombe|chr 2|||Manual
          Length = 608

 Score =  396 bits (976), Expect = e-111
 Identities = 186/270 (68%), Positives = 220/270 (81%), Gaps = 2/270 (0%)
 Frame = +3

Query: 81   LDPVGKWSKVGLVVYSSTVPIGATPEYLAGHYILQGASSFLPVMALAPQENERILDMCAA 260
            L+P+GKWSKVGL V+ S VPIGATPEYLAGHYILQ ASSFLPVMALAPQ NERILDM +A
Sbjct: 290  LEPIGKWSKVGLQVFESQVPIGATPEYLAGHYILQAASSFLPVMALAPQPNERILDMSSA 349

Query: 261  PGGKASHIAAIMKNTGALFANDANKERTKAIVGNFHRLGVVNTVICNYDGRQFP-DVIKG 437
            PGGK +++AA+ KNTG +FAND+NK RTKA+  N HRLGV N ++CNYDGR+FP +VI G
Sbjct: 350  PGGKVTYVAALQKNTGIIFANDSNKARTKALSANIHRLGVRNAIVCNYDGRKFPNEVIGG 409

Query: 438  FDRVLLDAPCTGTGVIAKDPSVKTTKDQKDIQRCFNLQRQLLLAAIDCCNAKSSTGSYIV 617
            FDRVLLDAPC+GTGVI KD SVKT K ++D     +LQRQLLL+AID  NA S TG +IV
Sbjct: 410  FDRVLLDAPCSGTGVIYKDQSVKTNKSERDFDTLSHLQRQLLLSAIDSVNADSKTGGFIV 469

Query: 618  YSTCSILPEENEWVVNYALKRR-NVKLVPTGLDFGTEGFVKYRHHRFHPSLKLTRRFYPH 794
            YSTCSI  +E+E V+ YALK+R NVKLV TGL+FG EGF ++R  RFHPSLKLTRR+YPH
Sbjct: 470  YSTCSITVDEDEAVIQYALKKRPNVKLVSTGLEFGREGFTRFREKRFHPSLKLTRRYYPH 529

Query: 795  THNMDGFFVAKLRKFSNVIPXPVXDDDXEE 884
             HN+DGFFVAKL+K S+ IP     DD ++
Sbjct: 530  VHNIDGFFVAKLKKISDKIPTVNVADDMKD 559


>SPAC23C4.17 |||tRNA |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 674

 Score = 81.4 bits (192), Expect = 3e-16
 Identities = 67/206 (32%), Positives = 94/206 (45%), Gaps = 35/206 (16%)
 Frame = +3

Query: 165 AGHYILQGASSFLPVMALAPQENERILDMCAAPGGKASH-IAAIMKNT------------ 305
           AG+   Q A S LP + L  + +  ILDMCAAPG K +  I A+ K              
Sbjct: 138 AGNINRQEAVSMLPPLFLDVEPHHVILDMCAAPGSKTAQLIEAVYKKANIKDAAHDSKNL 197

Query: 306 ----GALFANDANKERTKAIVGNFHRLGVVNTVICNYDGRQFPDV-IKG----------- 437
               G + ANDA+ +R + +V   +RL   N ++ N+D    P++ +KG           
Sbjct: 198 KSVEGLVIANDADPKRAQMLVHQINRLNSPNILVVNHDASTMPNIYVKGSSPSDGLNVIE 257

Query: 438 ------FDRVLLDAPCTGTGVIAKDPSVKTTKDQKDIQRCFNLQRQLLLAAIDCCNAKSS 599
                 FDR+L D PC+G G   K+ S+              LQ ++L+  +        
Sbjct: 258 EKKILKFDRILADVPCSGDGTFRKNLSLWREWSANSAFSLHPLQLRILIRGLQLLKV--- 314

Query: 600 TGSYIVYSTCSILPEENEWVVNYALK 677
            G  +VYSTCSI P ENE VV  ALK
Sbjct: 315 -GGCLVYSTCSINPIENEAVVTAALK 339



 Score = 29.9 bits (64), Expect = 0.94
 Identities = 12/19 (63%), Positives = 13/19 (68%)
 Frame = +3

Query: 780 RFYPHTHNMDGFFVAKLRK 836
           R YPH  N  GFFVA L+K
Sbjct: 411 RLYPHMQNTGGFFVAVLQK 429


>SPAC17D4.04 ||SPAC458.01|tRNA |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 654

 Score = 79.4 bits (187), Expect = 1e-15
 Identities = 63/201 (31%), Positives = 94/201 (46%), Gaps = 23/201 (11%)
 Frame = +3

Query: 165 AGHYILQGASSFLPVMALAPQENERILDMCAAPGGK-ASHIAAIMKNT-----------G 308
           AG    Q + S +P + L  + + ++LDMCAAPG K A  + A+ K T           G
Sbjct: 122 AGDINRQESVSMVPPLLLNVESHHKVLDMCAAPGSKTAQLLEALHKPTKKEDITTLLPSG 181

Query: 309 ALFANDANKERTKAIVGNFHRLGVVNTVICNYDGRQFPDV---------IKGFDRVLLDA 461
            + AND++ +R   +V    RL   N +I N+D    P+             FDR+L D 
Sbjct: 182 IVIANDSDNKRAHMLVHQIKRLNSPNVLIVNHDASFLPNFHLSSPDGKKFLKFDRILADV 241

Query: 462 PCTGTGVIAKDPSVKTTKDQKDIQRCFNLQRQLLLAAIDCCNAKSSTGSYIVYSTCSILP 641
           PC+G G   K+ ++      K        Q ++L+  +         G  +VYSTCS+ P
Sbjct: 242 PCSGDGTFRKNIALWNEWSLKTALGLHATQIKILMRGLQLL----EKGGRLVYSTCSLNP 297

Query: 642 EENEWVVNYALK--RRNVKLV 698
            ENE VV+  L   R +V+LV
Sbjct: 298 IENEAVVSAVLNATRGSVRLV 318



 Score = 33.1 bits (72), Expect = 0.10
 Identities = 13/23 (56%), Positives = 15/23 (65%)
 Frame = +3

Query: 780 RFYPHTHNMDGFFVAKLRKFSNV 848
           R YPH  N  GFFVA L K+ N+
Sbjct: 382 RIYPHFQNTGGFFVAVLEKYENL 404


>SPAC2C4.06c |||rRNA methyltransferase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 455

 Score = 67.7 bits (158), Expect = 4e-12
 Identities = 73/283 (25%), Positives = 121/283 (42%), Gaps = 18/283 (6%)
 Frame = +3

Query: 114 LVVYSSTVPIGATPEYLAGHYILQGASSFLPVMALAPQENE--RILDMCAAPGGKASHIA 287
           L+    + PI     Y  G  I+Q  +S  P   LA        I+D CAAPG K +H+A
Sbjct: 170 LIAIDPSFPIVENSLYKEGKVIIQDKASCFPAAVLAGLTGHVGDIIDGCAAPGNKTTHLA 229

Query: 288 AIMKNTGALFANDANKERTKAIVGNFHRLGVVNTVICNYD---GRQFPDVIKGFDRVLLD 458
           A    +  +FA + + +R + +       G  N  I + D        D+ +    +LLD
Sbjct: 230 ACFPKS-HIFAFERDAKRVQTLRKMVGISGANNVTIEHQDFTLTDPKSDLYRNVTHILLD 288

Query: 459 APCTGTGVIAKDPSV-----KTTKDQKDIQRCFNLQRQLLLAAIDCCNAKSSTGSYIVYS 623
             C+G+G++++   +       T+D + ++   + Q  +L  A+   N +     ++ YS
Sbjct: 289 PSCSGSGIVSRQDYLLGNEQDVTEDTERLENLCSFQSTILKHALQFPNCR-----HVTYS 343

Query: 624 TCSILPEENEWVVNYALKR----RNVKLVPTGLDFGTEGFVKYRHHRFHPSL-KLTRRFY 788
           TCS+   ENE VV   L +    +   L  T  ++ T G  +Y      PS+ +   R  
Sbjct: 344 TCSVHRLENEQVVCEVLSQEPDWKCNSLTKTLPNWKTRGIPEY---CAQPSMAEGMIRCK 400

Query: 789 PHTHNMDGFFVAKL---RKFSNVIPXPVXDDDXEEIEGKPSDK 908
           P      GFFVA L   ++          DDD ++ + K   K
Sbjct: 401 PGAGGTIGFFVANLYHPQREQETFKMYKNDDDTKKRKRKKKKK 443


>SPAC13F5.03c |||glycerol dehydrogenase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 450

 Score = 27.9 bits (59), Expect = 3.8
 Identities = 18/49 (36%), Positives = 26/49 (53%), Gaps = 4/49 (8%)
 Frame = -3

Query: 313 KAPVFFIIAAICDAL----PPGAAHISNILSFS*GAKAITGKKLEAPCK 179
           +APV F+I+ I DAL       +   SN  SF+ G  +I G+ +   CK
Sbjct: 220 RAPVRFLISGIGDALSTWVETESVIRSNSTSFAGGVASIAGRYIARACK 268


>SPBC337.16 |cho1||phosphatidyl-N-methylethanolamine
           N-methyltransferase |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 221

 Score = 26.6 bits (56), Expect = 8.7
 Identities = 11/38 (28%), Positives = 22/38 (57%)
 Frame = +3

Query: 351 IVGNFHRLGVVNTVICNYDGRQFPDVIKGFDRVLLDAP 464
           ++ + ++LG+V T + +Y G   P+ + GF   + D P
Sbjct: 125 VLSSMYKLGLVGTYLGDYFGFLLPERVSGFPFNVNDNP 162


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 4,851,200
Number of Sequences: 5004
Number of extensions: 96373
Number of successful extensions: 258
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 238
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 250
length of database: 2,362,478
effective HSP length: 76
effective length of database: 1,982,174
effective search space used: 826566558
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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