BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= 030725E6_A08_e537_02.seq (1482 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBP8B7.20c |||RNA methyltransferase Nop2 |Schizosaccharomyces p... 396 e-111 SPAC23C4.17 |||tRNA |Schizosaccharomyces pombe|chr 1|||Manual 81 3e-16 SPAC17D4.04 ||SPAC458.01|tRNA |Schizosaccharomyces pombe|chr 1||... 79 1e-15 SPAC2C4.06c |||rRNA methyltransferase |Schizosaccharomyces pombe... 68 4e-12 SPAC13F5.03c |||glycerol dehydrogenase |Schizosaccharomyces pomb... 28 3.8 SPBC337.16 |cho1||phosphatidyl-N-methylethanolamine N-methyltran... 27 8.7 >SPBP8B7.20c |||RNA methyltransferase Nop2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 608 Score = 396 bits (976), Expect = e-111 Identities = 186/270 (68%), Positives = 220/270 (81%), Gaps = 2/270 (0%) Frame = +3 Query: 81 LDPVGKWSKVGLVVYSSTVPIGATPEYLAGHYILQGASSFLPVMALAPQENERILDMCAA 260 L+P+GKWSKVGL V+ S VPIGATPEYLAGHYILQ ASSFLPVMALAPQ NERILDM +A Sbjct: 290 LEPIGKWSKVGLQVFESQVPIGATPEYLAGHYILQAASSFLPVMALAPQPNERILDMSSA 349 Query: 261 PGGKASHIAAIMKNTGALFANDANKERTKAIVGNFHRLGVVNTVICNYDGRQFP-DVIKG 437 PGGK +++AA+ KNTG +FAND+NK RTKA+ N HRLGV N ++CNYDGR+FP +VI G Sbjct: 350 PGGKVTYVAALQKNTGIIFANDSNKARTKALSANIHRLGVRNAIVCNYDGRKFPNEVIGG 409 Query: 438 FDRVLLDAPCTGTGVIAKDPSVKTTKDQKDIQRCFNLQRQLLLAAIDCCNAKSSTGSYIV 617 FDRVLLDAPC+GTGVI KD SVKT K ++D +LQRQLLL+AID NA S TG +IV Sbjct: 410 FDRVLLDAPCSGTGVIYKDQSVKTNKSERDFDTLSHLQRQLLLSAIDSVNADSKTGGFIV 469 Query: 618 YSTCSILPEENEWVVNYALKRR-NVKLVPTGLDFGTEGFVKYRHHRFHPSLKLTRRFYPH 794 YSTCSI +E+E V+ YALK+R NVKLV TGL+FG EGF ++R RFHPSLKLTRR+YPH Sbjct: 470 YSTCSITVDEDEAVIQYALKKRPNVKLVSTGLEFGREGFTRFREKRFHPSLKLTRRYYPH 529 Query: 795 THNMDGFFVAKLRKFSNVIPXPVXDDDXEE 884 HN+DGFFVAKL+K S+ IP DD ++ Sbjct: 530 VHNIDGFFVAKLKKISDKIPTVNVADDMKD 559 >SPAC23C4.17 |||tRNA |Schizosaccharomyces pombe|chr 1|||Manual Length = 674 Score = 81.4 bits (192), Expect = 3e-16 Identities = 67/206 (32%), Positives = 94/206 (45%), Gaps = 35/206 (16%) Frame = +3 Query: 165 AGHYILQGASSFLPVMALAPQENERILDMCAAPGGKASH-IAAIMKNT------------ 305 AG+ Q A S LP + L + + ILDMCAAPG K + I A+ K Sbjct: 138 AGNINRQEAVSMLPPLFLDVEPHHVILDMCAAPGSKTAQLIEAVYKKANIKDAAHDSKNL 197 Query: 306 ----GALFANDANKERTKAIVGNFHRLGVVNTVICNYDGRQFPDV-IKG----------- 437 G + ANDA+ +R + +V +RL N ++ N+D P++ +KG Sbjct: 198 KSVEGLVIANDADPKRAQMLVHQINRLNSPNILVVNHDASTMPNIYVKGSSPSDGLNVIE 257 Query: 438 ------FDRVLLDAPCTGTGVIAKDPSVKTTKDQKDIQRCFNLQRQLLLAAIDCCNAKSS 599 FDR+L D PC+G G K+ S+ LQ ++L+ + Sbjct: 258 EKKILKFDRILADVPCSGDGTFRKNLSLWREWSANSAFSLHPLQLRILIRGLQLLKV--- 314 Query: 600 TGSYIVYSTCSILPEENEWVVNYALK 677 G +VYSTCSI P ENE VV ALK Sbjct: 315 -GGCLVYSTCSINPIENEAVVTAALK 339 Score = 29.9 bits (64), Expect = 0.94 Identities = 12/19 (63%), Positives = 13/19 (68%) Frame = +3 Query: 780 RFYPHTHNMDGFFVAKLRK 836 R YPH N GFFVA L+K Sbjct: 411 RLYPHMQNTGGFFVAVLQK 429 >SPAC17D4.04 ||SPAC458.01|tRNA |Schizosaccharomyces pombe|chr 1|||Manual Length = 654 Score = 79.4 bits (187), Expect = 1e-15 Identities = 63/201 (31%), Positives = 94/201 (46%), Gaps = 23/201 (11%) Frame = +3 Query: 165 AGHYILQGASSFLPVMALAPQENERILDMCAAPGGK-ASHIAAIMKNT-----------G 308 AG Q + S +P + L + + ++LDMCAAPG K A + A+ K T G Sbjct: 122 AGDINRQESVSMVPPLLLNVESHHKVLDMCAAPGSKTAQLLEALHKPTKKEDITTLLPSG 181 Query: 309 ALFANDANKERTKAIVGNFHRLGVVNTVICNYDGRQFPDV---------IKGFDRVLLDA 461 + AND++ +R +V RL N +I N+D P+ FDR+L D Sbjct: 182 IVIANDSDNKRAHMLVHQIKRLNSPNVLIVNHDASFLPNFHLSSPDGKKFLKFDRILADV 241 Query: 462 PCTGTGVIAKDPSVKTTKDQKDIQRCFNLQRQLLLAAIDCCNAKSSTGSYIVYSTCSILP 641 PC+G G K+ ++ K Q ++L+ + G +VYSTCS+ P Sbjct: 242 PCSGDGTFRKNIALWNEWSLKTALGLHATQIKILMRGLQLL----EKGGRLVYSTCSLNP 297 Query: 642 EENEWVVNYALK--RRNVKLV 698 ENE VV+ L R +V+LV Sbjct: 298 IENEAVVSAVLNATRGSVRLV 318 Score = 33.1 bits (72), Expect = 0.10 Identities = 13/23 (56%), Positives = 15/23 (65%) Frame = +3 Query: 780 RFYPHTHNMDGFFVAKLRKFSNV 848 R YPH N GFFVA L K+ N+ Sbjct: 382 RIYPHFQNTGGFFVAVLEKYENL 404 >SPAC2C4.06c |||rRNA methyltransferase |Schizosaccharomyces pombe|chr 1|||Manual Length = 455 Score = 67.7 bits (158), Expect = 4e-12 Identities = 73/283 (25%), Positives = 121/283 (42%), Gaps = 18/283 (6%) Frame = +3 Query: 114 LVVYSSTVPIGATPEYLAGHYILQGASSFLPVMALAPQENE--RILDMCAAPGGKASHIA 287 L+ + PI Y G I+Q +S P LA I+D CAAPG K +H+A Sbjct: 170 LIAIDPSFPIVENSLYKEGKVIIQDKASCFPAAVLAGLTGHVGDIIDGCAAPGNKTTHLA 229 Query: 288 AIMKNTGALFANDANKERTKAIVGNFHRLGVVNTVICNYD---GRQFPDVIKGFDRVLLD 458 A + +FA + + +R + + G N I + D D+ + +LLD Sbjct: 230 ACFPKS-HIFAFERDAKRVQTLRKMVGISGANNVTIEHQDFTLTDPKSDLYRNVTHILLD 288 Query: 459 APCTGTGVIAKDPSV-----KTTKDQKDIQRCFNLQRQLLLAAIDCCNAKSSTGSYIVYS 623 C+G+G++++ + T+D + ++ + Q +L A+ N + ++ YS Sbjct: 289 PSCSGSGIVSRQDYLLGNEQDVTEDTERLENLCSFQSTILKHALQFPNCR-----HVTYS 343 Query: 624 TCSILPEENEWVVNYALKR----RNVKLVPTGLDFGTEGFVKYRHHRFHPSL-KLTRRFY 788 TCS+ ENE VV L + + L T ++ T G +Y PS+ + R Sbjct: 344 TCSVHRLENEQVVCEVLSQEPDWKCNSLTKTLPNWKTRGIPEY---CAQPSMAEGMIRCK 400 Query: 789 PHTHNMDGFFVAKL---RKFSNVIPXPVXDDDXEEIEGKPSDK 908 P GFFVA L ++ DDD ++ + K K Sbjct: 401 PGAGGTIGFFVANLYHPQREQETFKMYKNDDDTKKRKRKKKKK 443 >SPAC13F5.03c |||glycerol dehydrogenase |Schizosaccharomyces pombe|chr 1|||Manual Length = 450 Score = 27.9 bits (59), Expect = 3.8 Identities = 18/49 (36%), Positives = 26/49 (53%), Gaps = 4/49 (8%) Frame = -3 Query: 313 KAPVFFIIAAICDAL----PPGAAHISNILSFS*GAKAITGKKLEAPCK 179 +APV F+I+ I DAL + SN SF+ G +I G+ + CK Sbjct: 220 RAPVRFLISGIGDALSTWVETESVIRSNSTSFAGGVASIAGRYIARACK 268 >SPBC337.16 |cho1||phosphatidyl-N-methylethanolamine N-methyltransferase |Schizosaccharomyces pombe|chr 2|||Manual Length = 221 Score = 26.6 bits (56), Expect = 8.7 Identities = 11/38 (28%), Positives = 22/38 (57%) Frame = +3 Query: 351 IVGNFHRLGVVNTVICNYDGRQFPDVIKGFDRVLLDAP 464 ++ + ++LG+V T + +Y G P+ + GF + D P Sbjct: 125 VLSSMYKLGLVGTYLGDYFGFLLPERVSGFPFNVNDNP 162 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 4,851,200 Number of Sequences: 5004 Number of extensions: 96373 Number of successful extensions: 258 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 238 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 250 length of database: 2,362,478 effective HSP length: 76 effective length of database: 1,982,174 effective search space used: 826566558 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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