Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030725E6_A07_e529_01.seq
(1524 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC26F1.07 |||2-methylbutyraldehyde reductase |Schizosaccharomy... 151 3e-37
SPBC8E4.04 |||aldo/keto reductase involved in pentose catabolism... 138 2e-33
SPAC2F3.05c |||xylose and arabinose reductase |Schizosaccharomyc... 97 4e-21
SPAP32A8.02 |||xylose and arabinose reductase |Schizosaccharomyc... 96 1e-20
SPAC19G12.09 |||NADH/NADPH dependent indole-3-acetaldehyde reduc... 91 3e-19
SPBC28F2.05c |||xylose and arabinose reductase |Schizosaccharomy... 63 1e-10
SPCC737.06c |||glutamate-cysteine ligase regulatory subunit |Sch... 46 1e-05
SPAC1F7.12 |yak3|yakC, SPAC21E11.01|aldose reductase YakC|Schizo... 37 0.006
SPCC18.04 |pof6||F-box protein Pof6|Schizosaccharomyces pombe|ch... 27 6.8
SPBC3E7.11c |||DNAJ protein Caj1/Djp1-type|Schizosaccharomyces p... 27 9.0
>SPAC26F1.07 |||2-methylbutyraldehyde reductase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 321
Score = 151 bits (365), Expect = 3e-37
Identities = 78/194 (40%), Positives = 117/194 (60%), Gaps = 2/194 (1%)
Frame = +2
Query: 155 REDLFITSKLWNTFHRPDLXRGALEVSLRNLNIDYLDLYLIHWPQAYKEDDAIFPTDADG 334
R+D+++TSKLW H P+ ALE +L++L +DYLD YLIHWP ++K + FP D DG
Sbjct: 77 RKDIWVTSKLWCNAHAPEAVPKALEKTLKDLKLDYLDEYLIHWPVSFKTGEDKFPKDKDG 136
Query: 335 KIQFSDVDYVDTWKALEPLVCHGLVRSLGVSNFNSKQLQRILDNASVKPVTNQVECHPXL 514
+ + +TWKA+E L+ G VR +G+SNFN L+RIL A VKP +Q+E HP L
Sbjct: 137 NLIYEKNPIEETWKAMEKLLETGKVRHIGLSNFNDTNLERILKVAKVKPAVHQMELHPFL 196
Query: 515 NQKRLKQFCEDRDIMITAYSPLGSPDRPWAKPGDPQLMEDPKLKEIADRLGK--TVAQVL 688
Q + + I +TAYSP G+ + + + P+L+E +++IA G+ T A +
Sbjct: 197 PQTEFVEKHKKLGIHVTAYSPFGNQNTIY-ESKIPKLIEHETIQKIAKSKGEGVTGATIA 255
Query: 689 IRYQIDRGNIXIPE 730
+ + I RG IP+
Sbjct: 256 VSWAITRGTSVIPK 269
>SPBC8E4.04 |||aldo/keto reductase involved in pentose catabolism
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 325
Score = 138 bits (333), Expect = 2e-33
Identities = 73/194 (37%), Positives = 114/194 (58%), Gaps = 2/194 (1%)
Frame = +2
Query: 155 REDLFITSKLWNTFHRPDLXRGALEVSLRNLNIDYLDLYLIHWPQAYKEDDAIFPTDADG 334
R D+++TSKLW HR L ALE +L++LN++Y+D YLIHWP A P + G
Sbjct: 76 RTDIWVTSKLWCNAHRAGLVPLALEKTLQDLNLEYIDAYLIHWPFALLSGPEELPRNEKG 135
Query: 335 KIQFSDVDYVDTWKALEPLVCHGLVRSLGVSNFNSKQLQRILDNASVKPVTNQVECHPXL 514
++ + DV +TW+A+E L+ G VR +G+SNFN++ L R+L A VKP +Q+E HP L
Sbjct: 136 ELIYEDVPIEETWQAMEELLETGKVRYIGISNFNNEYLDRVLKIAKVKPTIHQMELHPYL 195
Query: 515 NQKRLKQFCEDRDIMITAYSPLGSPDRPWAKPGDPQLMEDPKLKEIADRLGK--TVAQVL 688
Q + + I ++AYSPL + + + +L+E L +IA+ G+ T A +
Sbjct: 196 PQTEYLEKHKKLQIHVSAYSPLANQNDAY-NSDISKLIEHKTLVDIANARGEGITPANIA 254
Query: 689 IRYQIDRGNIXIPE 730
I + + RG +P+
Sbjct: 255 ISWAVKRGTSVLPK 268
>SPAC2F3.05c |||xylose and arabinose reductase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 275
Score = 97.5 bits (232), Expect = 4e-21
Identities = 63/194 (32%), Positives = 103/194 (53%), Gaps = 1/194 (0%)
Frame = +2
Query: 152 KREDLFITSKLWNTFHRPDLXRGALEVSLRNLNIDYLDLYLIHWPQAYKEDDAIFPTDAD 331
KRED++ TSKL N +++ S++ + Y+DL+L+H P
Sbjct: 70 KREDIWFTSKL-NDLSGYKSTLSSIDASVKACGLGYIDLFLLHSPYG------------- 115
Query: 332 GKIQFSDVDYVDTWKALEPLVCHGLVRSLGVSNFNSKQLQRILDN-ASVKPVTNQVECHP 508
D +++WKALE V G +R++GVSNF +Q +LD+ + P NQ+E HP
Sbjct: 116 --------DRIESWKALEKGVEEGKLRAIGVSNFGPHHIQELLDSHPKIIPCVNQIELHP 167
Query: 509 XLNQKRLKQFCEDRDIMITAYSPLGSPDRPWAKPGDPQLMEDPKLKEIADRLGKTVAQVL 688
+Q+++ +CE + I + AY+PL + K G+ QL+ IA + K+ AQ++
Sbjct: 168 FCSQQKVVDYCESKGIQLAAYAPLVHGE----KFGNKQLL------AIASKYNKSEAQIM 217
Query: 689 IRYQIDRGNIXIPE 730
IRY + RG I +P+
Sbjct: 218 IRYCLQRGFIVLPK 231
Score = 32.3 bits (70), Expect = 0.18
Identities = 14/25 (56%), Positives = 18/25 (72%)
Frame = +3
Query: 726 PKSVTKSRIASNFDVFDFKLSLQDV 800
PKS T RI N DVFDF++S +D+
Sbjct: 230 PKSSTPRRIKENGDVFDFEISKEDM 254
>SPAP32A8.02 |||xylose and arabinose reductase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 283
Score = 96.3 bits (229), Expect = 1e-20
Identities = 72/192 (37%), Positives = 98/192 (51%), Gaps = 1/192 (0%)
Frame = +2
Query: 152 KREDLFITSKLWNTFHRPDLXRGALEVSLRNLNIDYLDLYLIHWPQAYKEDDAIFPTDAD 331
KR D+F+TSKL N R A+ SL +L Y+DL+LI P A
Sbjct: 75 KRTDIFLTSKLANCSDYYS-TRAAIRSSLHHLGT-YIDLFLIQSP-------------AG 119
Query: 332 GKIQFSDVDYVDTWKALEPLVCHGLVRSLGVSNFNSKQLQRIL-DNASVKPVTNQVECHP 508
GK + +WKA+E V G +RS+GVSN+ K LQ + N P NQ+E HP
Sbjct: 120 GK-----KSRIASWKAMEEFVDSGDIRSVGVSNYGVKHLQELYASNPKFYPCVNQIELHP 174
Query: 509 XLNQKRLKQFCEDRDIMITAYSPLGSPDRPWAKPGDPQLMEDPKLKEIADRLGKTVAQVL 688
L+Q + ++C+ DI I AYSPL R + D KL IA +L +VAQ+L
Sbjct: 175 FLSQDDIVKYCQSHDIAIEAYSPLTHGIR----------LNDEKLVPIAKKLNISVAQLL 224
Query: 689 IRYQIDRGNIXI 724
IR+ + +G I I
Sbjct: 225 IRWSLQKGYIPI 236
>SPAC19G12.09 |||NADH/NADPH dependent indole-3-acetaldehyde
reductase AKR3C2|Schizosaccharomyces pombe|chr
1|||Manual
Length = 284
Score = 91.5 bits (217), Expect = 3e-19
Identities = 67/192 (34%), Positives = 95/192 (49%), Gaps = 2/192 (1%)
Frame = +2
Query: 155 REDLFITSKLWNTFHRPDLXRGALEVSLRNLNIDYLDLYLIHWPQAYKEDDAIFPTDADG 334
R LFITSK+ H D AL SLR L DYLDLYL+H P + E
Sbjct: 71 RSKLFITSKV---MHNVDNIPEALNESLRKLGTDYLDLYLLHSPIPFYEK---------- 117
Query: 335 KIQFSDVDYVDTWKALEPLVCHGLVRSLGVSNFNSKQLQRILDNASVKPVTNQVECHPXL 514
KI S+ WKA+E + GLV S+GVSNF L+ +L +++ P NQ+E HP +
Sbjct: 118 KIPISE-----GWKAMETALGTGLVHSVGVSNFRIPDLEELLKTSTITPRVNQIEFHPQV 172
Query: 515 NQ--KRLKQFCEDRDIMITAYSPLGSPDRPWAKPGDPQLMEDPKLKEIADRLGKTVAQVL 688
+ K L +FC+ + I++ Y PL P + + E K E + T Q+L
Sbjct: 173 YKAAKPLVEFCQSKGIIVEGYGPLS----PLVRDAQGPVAEFTKSLESKYHVSDT--QIL 226
Query: 689 IRYQIDRGNIXI 724
+++ +G I I
Sbjct: 227 LKWAYSKGVIPI 238
>SPBC28F2.05c |||xylose and arabinose reductase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 276
Score = 62.9 bits (146), Expect = 1e-10
Identities = 51/187 (27%), Positives = 87/187 (46%)
Frame = +2
Query: 152 KREDLFITSKLWNTFHRPDLXRGALEVSLRNLNIDYLDLYLIHWPQAYKEDDAIFPTDAD 331
KRED+ + +K+ ++ + A+E SLR LD+ LIH P+ +P
Sbjct: 68 KREDITLITKIPDSLQGFERTWKAVEQSLRRTGRPKLDVVLIHSPK--------WP---- 115
Query: 332 GKIQFSDVDYVDTWKALEPLVCHGLVRSLGVSNFNSKQLQRILDNASVKPVTNQVECHPX 511
V +++W+AL G + +GVSN+N L+ I+ P NQVE
Sbjct: 116 -------VRRIESWRALLQHQKEGRINKIGVSNYNIHHLEEIISLGLPLPAINQVEFSAF 168
Query: 512 LNQKRLKQFCEDRDIMITAYSPLGSPDRPWAKPGDPQLMEDPKLKEIADRLGKTVAQVLI 691
N+ +C + I++ A+SPL R + D +L +++ + KT A +L+
Sbjct: 169 NNRPTFLSYCFNHGILVQAFSPLTRGYR----------LSDIRLLDLSLKYNKTPANILL 218
Query: 692 RYQIDRG 712
RY + +G
Sbjct: 219 RYCLQKG 225
>SPCC737.06c |||glutamate-cysteine ligase regulatory subunit
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 287
Score = 46.4 bits (105), Expect = 1e-05
Identities = 20/46 (43%), Positives = 30/46 (65%)
Frame = +2
Query: 359 YVDTWKALEPLVCHGLVRSLGVSNFNSKQLQRILDNASVKPVTNQV 496
+VDTWK LE V G + +LGVS F +LQR++ + +V P + Q+
Sbjct: 158 WVDTWKLLEEKVGEGKIGTLGVSEFGVNELQRLISSVNVVPESTQI 203
>SPAC1F7.12 |yak3|yakC, SPAC21E11.01|aldose reductase
YakC|Schizosaccharomyces pombe|chr 1|||Manual
Length = 340
Score = 37.1 bits (82), Expect = 0.006
Identities = 40/136 (29%), Positives = 65/136 (47%), Gaps = 7/136 (5%)
Frame = +2
Query: 197 HRPDLXRGALEVSLRNLNIDYLDLYLIHWPQAYKEDDAIFPTDADGKIQFSDVDYVD-TW 373
+ PD AL++SL+ L ID +DLY +H +FS ++
Sbjct: 99 NEPDYIEKALDLSLKRLGIDCIDLYYVH--------------------RFSGETPIEKIM 138
Query: 374 KALEPLVCHGLVRSLGVSNFNSKQLQRILDNASVKPVTN-QVECHP-XLNQKR----LKQ 535
AL+ V G +R +G+S ++ ++R A+V PV+ QVE P L +R + +
Sbjct: 139 GALKKCVEAGKIRYIGLSECSANTIRRA---AAVYPVSAVQVEYSPFSLEIERPEIGVMK 195
Query: 536 FCEDRDIMITAYSPLG 583
C + +I I Y+PLG
Sbjct: 196 ACRENNITIVCYAPLG 211
>SPCC18.04 |pof6||F-box protein Pof6|Schizosaccharomyces pombe|chr
3|||Manual
Length = 872
Score = 27.1 bits (57), Expect = 6.8
Identities = 7/19 (36%), Positives = 15/19 (78%)
Frame = +2
Query: 335 KIQFSDVDYVDTWKALEPL 391
+++F+ +DY+ W+AL P+
Sbjct: 164 RVEFARLDYIKVWRALAPI 182
>SPBC3E7.11c |||DNAJ protein Caj1/Djp1-type|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 355
Score = 26.6 bits (56), Expect = 9.0
Identities = 11/20 (55%), Positives = 15/20 (75%)
Frame = +2
Query: 620 QLMEDPKLKEIADRLGKTVA 679
Q++ DPKL+E D+LGK A
Sbjct: 60 QVLSDPKLREKYDKLGKVGA 79
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 4,164,103
Number of Sequences: 5004
Number of extensions: 70063
Number of successful extensions: 162
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 147
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 155
length of database: 2,362,478
effective HSP length: 76
effective length of database: 1,982,174
effective search space used: 854316994
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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