Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030725E6_A06_e521_02.seq
(1525 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g05630.1 68415.m00599 autophagy 8d (APG8d) identical to autop... 158 8e-39
At4g16520.2 68417.m02501 autophagy 8f (APG8f) identical to autop... 154 2e-37
At4g16520.1 68417.m02500 autophagy 8f (APG8f) identical to autop... 154 2e-37
At2g45170.2 68415.m05624 autophagy 8e (APG8e) identical to autop... 151 1e-36
At2g45170.1 68415.m05623 autophagy 8e (APG8e) identical to autop... 151 1e-36
At3g60640.1 68416.m06785 autophagy 8g (APG8g) identical to autop... 150 3e-36
At4g21980.1 68417.m03182 autophagy 8a (APG8a) identical to autop... 145 6e-35
At1g62040.1 68414.m06997 autophagy 8c (APG8c) identical to autop... 144 1e-34
At4g04620.2 68417.m00676 autophagy 8b (APG8b) identical to autop... 143 3e-34
At4g04620.1 68417.m00675 autophagy 8b (APG8b) identical to autop... 143 3e-34
At3g15580.1 68416.m01974 autophagy 8i (APG8i) identical to autop... 120 3e-27
At3g06420.1 68416.m00740 autophagy 8h (APG8h) identical to autop... 118 1e-26
At2g43690.1 68415.m05431 lectin protein kinase, putative similar... 30 3.5
At3g60120.1 68416.m06713 glycosyl hydrolase family 1 protein con... 30 4.6
At3g19170.1 68416.m02434 peptidase M16 family protein / insulina... 30 4.6
At3g47400.1 68416.m05154 pectinesterase family protein similar t... 29 6.1
>At2g05630.1 68415.m00599 autophagy 8d (APG8d) identical to
autophagy 8d [Arabidopsis thaliana] GI:19912157;
contains Pfam profile PF02991: Microtubule associated
protein 1A/1B, light chain 3
Length = 120
Score = 158 bits (384), Expect = 8e-39
Identities = 67/115 (58%), Positives = 91/115 (79%)
Frame = +1
Query: 178 YKEEHSFEKRKTEGEKIRRKYPDRVPVIVEKAPKARLGDLDKKKYLVPSDLTVGQFYFLI 357
+K EH EKR+ E +IR KYPDR+PVIVE+A K+ + D+D+KKYLVP+DLTVGQF +++
Sbjct: 6 FKHEHPLEKRQAEAARIREKYPDRIPVIVERAEKSDVPDIDRKKYLVPADLTVGQFVYVV 65
Query: 358 RKRIHLRPEDALFFFVNNVIPPTSATMGSLYQEHHDEDFFLYIAFSDENVYGY*F 522
RKRI L PE A+F FV N++PPT+A M ++Y+EH DED FLY+++S EN +G F
Sbjct: 66 RKRIKLSPEKAIFIFVKNILPPTAAIMSAIYEEHKDEDGFLYMSYSGENTFGIFF 120
>At4g16520.2 68417.m02501 autophagy 8f (APG8f) identical to
autophagy 8f [Arabidopsis thaliana] GI:19912161;
contains Pfam profile PF02991: Microtubule associated
protein 1A/1B, light chain 3
Length = 121
Score = 154 bits (373), Expect = 2e-37
Identities = 67/116 (57%), Positives = 88/116 (75%)
Frame = +1
Query: 169 KFQYKEEHSFEKRKTEGEKIRRKYPDRVPVIVEKAPKARLGDLDKKKYLVPSDLTVGQFY 348
K +K+EH EKR+ E +IR KYPDR+PVIVEKA K+ + +DKKKYLVP+DLTVGQF
Sbjct: 3 KSSFKQEHDLEKRRAEAARIREKYPDRIPVIVEKAEKSDIPTIDKKKYLVPADLTVGQFV 62
Query: 349 FLIRKRIHLRPEDALFFFVNNVIPPTSATMGSLYQEHHDEDFFLYIAFSDENVYGY 516
++IRKRI L E A+F FV+NV+PP A M S+Y+E D+D FLY+ +S EN +G+
Sbjct: 63 YVIRKRIKLSAEKAIFIFVDNVLPPAGALMSSVYEEKKDDDGFLYVTYSGENTFGF 118
>At4g16520.1 68417.m02500 autophagy 8f (APG8f) identical to
autophagy 8f [Arabidopsis thaliana] GI:19912161;
contains Pfam profile PF02991: Microtubule associated
protein 1A/1B, light chain 3
Length = 121
Score = 154 bits (373), Expect = 2e-37
Identities = 67/116 (57%), Positives = 88/116 (75%)
Frame = +1
Query: 169 KFQYKEEHSFEKRKTEGEKIRRKYPDRVPVIVEKAPKARLGDLDKKKYLVPSDLTVGQFY 348
K +K+EH EKR+ E +IR KYPDR+PVIVEKA K+ + +DKKKYLVP+DLTVGQF
Sbjct: 3 KSSFKQEHDLEKRRAEAARIREKYPDRIPVIVEKAEKSDIPTIDKKKYLVPADLTVGQFV 62
Query: 349 FLIRKRIHLRPEDALFFFVNNVIPPTSATMGSLYQEHHDEDFFLYIAFSDENVYGY 516
++IRKRI L E A+F FV+NV+PP A M S+Y+E D+D FLY+ +S EN +G+
Sbjct: 63 YVIRKRIKLSAEKAIFIFVDNVLPPAGALMSSVYEEKKDDDGFLYVTYSGENTFGF 118
>At2g45170.2 68415.m05624 autophagy 8e (APG8e) identical to
autophagy 8e [Arabidopsis thaliana] GI:19912159;
contains Pfam profile PF02991: Microtubule associated
protein 1A/1B, light chain 3
Length = 122
Score = 151 bits (366), Expect = 1e-36
Identities = 68/112 (60%), Positives = 86/112 (76%)
Frame = +1
Query: 178 YKEEHSFEKRKTEGEKIRRKYPDRVPVIVEKAPKARLGDLDKKKYLVPSDLTVGQFYFLI 357
+K + FEKRK E +IR KYPDR+PVIVEKA K+ + ++DKKKYLVPSDLTVGQF ++I
Sbjct: 7 FKMDDDFEKRKAEAGRIREKYPDRIPVIVEKAEKSEVPNIDKKKYLVPSDLTVGQFVYVI 66
Query: 358 RKRIHLRPEDALFFFVNNVIPPTSATMGSLYQEHHDEDFFLYIAFSDENVYG 513
RKRI L E A+F FV+NV+PPT M S+Y++ DED FLYI +S EN +G
Sbjct: 67 RKRIKLSAEKAIFIFVDNVLPPTGELMSSVYEDKKDEDGFLYITYSGENTFG 118
>At2g45170.1 68415.m05623 autophagy 8e (APG8e) identical to
autophagy 8e [Arabidopsis thaliana] GI:19912159;
contains Pfam profile PF02991: Microtubule associated
protein 1A/1B, light chain 3
Length = 122
Score = 151 bits (366), Expect = 1e-36
Identities = 68/112 (60%), Positives = 86/112 (76%)
Frame = +1
Query: 178 YKEEHSFEKRKTEGEKIRRKYPDRVPVIVEKAPKARLGDLDKKKYLVPSDLTVGQFYFLI 357
+K + FEKRK E +IR KYPDR+PVIVEKA K+ + ++DKKKYLVPSDLTVGQF ++I
Sbjct: 7 FKMDDDFEKRKAEAGRIREKYPDRIPVIVEKAEKSEVPNIDKKKYLVPSDLTVGQFVYVI 66
Query: 358 RKRIHLRPEDALFFFVNNVIPPTSATMGSLYQEHHDEDFFLYIAFSDENVYG 513
RKRI L E A+F FV+NV+PPT M S+Y++ DED FLYI +S EN +G
Sbjct: 67 RKRIKLSAEKAIFIFVDNVLPPTGELMSSVYEDKKDEDGFLYITYSGENTFG 118
>At3g60640.1 68416.m06785 autophagy 8g (APG8g) identical to
autophagy 8g [Arabidopsis thaliana] GI:19912163;
contains Pfam profile PF02991: Microtubule associated
protein 1A/1B, light chain 3; supporting cDNA
gi|19912162|dbj|AB073181.1|
Length = 121
Score = 150 bits (363), Expect = 3e-36
Identities = 65/112 (58%), Positives = 88/112 (78%)
Frame = +1
Query: 178 YKEEHSFEKRKTEGEKIRRKYPDRVPVIVEKAPKARLGDLDKKKYLVPSDLTVGQFYFLI 357
++++H FEKRK E +IR KY DRVPVIVEK+ K+ + ++DKKKYLVP+DLTVGQF ++I
Sbjct: 6 FRQDHDFEKRKAEALRIREKYSDRVPVIVEKSEKSDIPNIDKKKYLVPADLTVGQFVYVI 65
Query: 358 RKRIHLRPEDALFFFVNNVIPPTSATMGSLYQEHHDEDFFLYIAFSDENVYG 513
RKRI L E A+F FV+NV+PPT A M ++Y E+ +ED FLY+ +S EN +G
Sbjct: 66 RKRIQLSAEKAIFIFVDNVLPPTGAMMSTIYDENKEEDGFLYVTYSGENTFG 117
>At4g21980.1 68417.m03182 autophagy 8a (APG8a) identical to
autophagy 8a [Arabidopsis thaliana] GI:19912151;
contains Pfam profile PF02991: Microtubule associated
protein 1A/1B, light chain 3
Length = 122
Score = 145 bits (352), Expect = 6e-35
Identities = 64/115 (55%), Positives = 86/115 (74%)
Frame = +1
Query: 169 KFQYKEEHSFEKRKTEGEKIRRKYPDRVPVIVEKAPKARLGDLDKKKYLVPSDLTVGQFY 348
K +K + E R +E +IR KYPDR+PVIVEKA ++ + D+DKKKYLVP+DLTVGQF
Sbjct: 3 KSSFKISNPLEARMSESSRIREKYPDRIPVIVEKAGQSDVPDIDKKKYLVPADLTVGQFV 62
Query: 349 FLIRKRIHLRPEDALFFFVNNVIPPTSATMGSLYQEHHDEDFFLYIAFSDENVYG 513
+++RKRI L E A+F FV N +PPT+A M ++Y+EH DED FLY+ +S EN +G
Sbjct: 63 YVVRKRIKLGAEKAIFVFVKNTLPPTAALMSAIYEEHKDEDGFLYMTYSGENTFG 117
>At1g62040.1 68414.m06997 autophagy 8c (APG8c) identical to
autophagy 8c [Arabidopsis thaliana] GI:19912155;
contains Pfam profile PF02991: Microtubule associated
protein 1A/1B, light chain 3
Length = 119
Score = 144 bits (350), Expect = 1e-34
Identities = 62/112 (55%), Positives = 86/112 (76%)
Frame = +1
Query: 178 YKEEHSFEKRKTEGEKIRRKYPDRVPVIVEKAPKARLGDLDKKKYLVPSDLTVGQFYFLI 357
+K EH E+R+ E +IR KYPDR+PVIVE+A ++ + ++DKKKYLVP+DLTVGQF +++
Sbjct: 6 FKLEHPLERRQIESSRIREKYPDRIPVIVERAERSDVPNIDKKKYLVPADLTVGQFVYVV 65
Query: 358 RKRIHLRPEDALFFFVNNVIPPTSATMGSLYQEHHDEDFFLYIAFSDENVYG 513
RKRI L E A+F FV N +PPT+A M ++Y E+ DED FLY+ +S EN +G
Sbjct: 66 RKRIKLSAEKAIFVFVKNTLPPTAAMMSAIYDENKDEDGFLYMTYSGENTFG 117
>At4g04620.2 68417.m00676 autophagy 8b (APG8b) identical to
autophagy 8b [Arabidopsis thaliana] GI:19912153;
contains Pfam profile PF02991: Microtubule associated
protein 1A/1B, light chain 3
Length = 122
Score = 143 bits (346), Expect = 3e-34
Identities = 64/119 (53%), Positives = 87/119 (73%)
Frame = +1
Query: 169 KFQYKEEHSFEKRKTEGEKIRRKYPDRVPVIVEKAPKARLGDLDKKKYLVPSDLTVGQFY 348
K +K + E R E +IR KYP+RVPVIVEKA ++ + D+DKKKYLVP+DLT+GQF
Sbjct: 3 KNSFKLSNPLEMRMAESTRIRAKYPERVPVIVEKAGQSDVPDIDKKKYLVPADLTIGQFV 62
Query: 349 FLIRKRIHLRPEDALFFFVNNVIPPTSATMGSLYQEHHDEDFFLYIAFSDENVYGY*FF 525
+++RKRI L E A+F FV N +PPT+A M ++Y+EH DED FLY+ +S EN +G F+
Sbjct: 63 YVVRKRIKLGAEKAIFVFVKNTLPPTAALMSAIYEEHKDEDGFLYMTYSGENTFGGSFY 121
>At4g04620.1 68417.m00675 autophagy 8b (APG8b) identical to
autophagy 8b [Arabidopsis thaliana] GI:19912153;
contains Pfam profile PF02991: Microtubule associated
protein 1A/1B, light chain 3
Length = 122
Score = 143 bits (346), Expect = 3e-34
Identities = 64/119 (53%), Positives = 87/119 (73%)
Frame = +1
Query: 169 KFQYKEEHSFEKRKTEGEKIRRKYPDRVPVIVEKAPKARLGDLDKKKYLVPSDLTVGQFY 348
K +K + E R E +IR KYP+RVPVIVEKA ++ + D+DKKKYLVP+DLT+GQF
Sbjct: 3 KNSFKLSNPLEMRMAESTRIRAKYPERVPVIVEKAGQSDVPDIDKKKYLVPADLTIGQFV 62
Query: 349 FLIRKRIHLRPEDALFFFVNNVIPPTSATMGSLYQEHHDEDFFLYIAFSDENVYGY*FF 525
+++RKRI L E A+F FV N +PPT+A M ++Y+EH DED FLY+ +S EN +G F+
Sbjct: 63 YVVRKRIKLGAEKAIFVFVKNTLPPTAALMSAIYEEHKDEDGFLYMTYSGENTFGGSFY 121
>At3g15580.1 68416.m01974 autophagy 8i (APG8i) identical to
autophagy 8i [Arabidopsis thaliana] GI:19912167;
contains Pfam profile PF02991: Microtubule associated
protein 1A/1B, light chain 3; supporting cDNA
gi|21636957|gb|AF492760.1|
Length = 115
Score = 120 bits (289), Expect = 3e-27
Identities = 51/112 (45%), Positives = 78/112 (69%)
Frame = +1
Query: 178 YKEEHSFEKRKTEGEKIRRKYPDRVPVIVEKAPKARLGDLDKKKYLVPSDLTVGQFYFLI 357
+KE+++ ++R E +I KYP R+PVI EK K L ++KKK+LVP D++VGQF +++
Sbjct: 4 FKEQYTLDERLAESREIIAKYPTRIPVIAEKYCKTDLPAIEKKKFLVPRDMSVGQFIYIL 63
Query: 358 RKRIHLRPEDALFFFVNNVIPPTSATMGSLYQEHHDEDFFLYIAFSDENVYG 513
R+HL P ALF FVNN +P T+A M S+Y+ + D+D F+Y+ +S E +G
Sbjct: 64 SARLHLSPGKALFVFVNNTLPQTAALMDSVYESYKDDDGFVYMCYSSEKTFG 115
>At3g06420.1 68416.m00740 autophagy 8h (APG8h) identical to
autophagy 8h [Arabidopsis thaliana] GI:19912165;
contains Pfam profile PF02991: Microtubule associated
protein 1A/1B, light chain 3; supporting cDNA
gi|19912164|dbj|AB073182.1|
Length = 119
Score = 118 bits (283), Expect = 1e-26
Identities = 53/112 (47%), Positives = 74/112 (66%)
Frame = +1
Query: 178 YKEEHSFEKRKTEGEKIRRKYPDRVPVIVEKAPKARLGDLDKKKYLVPSDLTVGQFYFLI 357
+K++ S ++R E I KYPDR+PVI+EK A L D++K KYLVP D+TVG F ++
Sbjct: 8 FKDQFSSDERLKESNNIIAKYPDRIPVIIEKYSNADLPDMEKNKYLVPRDMTVGHFIHML 67
Query: 358 RKRIHLRPEDALFFFVNNVIPPTSATMGSLYQEHHDEDFFLYIAFSDENVYG 513
KR+ L P ALF FV+N +P T++ M SLY +ED FLY+ +S E +G
Sbjct: 68 SKRMQLDPSKALFVFVHNTLPQTASRMDSLYNTFKEEDGFLYMCYSTEKTFG 119
>At2g43690.1 68415.m05431 lectin protein kinase, putative similar to
receptor-like kinase LECRK1 [Arabidopsis thaliana]
gi|2150023|gb|AAB58725
Length = 664
Score = 30.3 bits (65), Expect = 3.5
Identities = 13/43 (30%), Positives = 23/43 (53%)
Frame = -3
Query: 269 FSTITGTRSGYFLRIFSPSVFLFSKECSSLYWNFIFVFFYAII 141
F+T+T T+ + + F+ F F + +F F FF+AI+
Sbjct: 40 FTTLTNTKKHAYGQAFNDEPFPFKNSVNGNMTSFSFTFFFAIV 82
>At3g60120.1 68416.m06713 glycosyl hydrolase family 1 protein
contains Pfam PF00232 : Glycosyl hydrolase family 1
domain; similar to anther-specific protein ATA27
(GI:2746341) [Arabidopsis thaliana]
Length = 534
Score = 29.9 bits (64), Expect = 4.6
Identities = 17/61 (27%), Positives = 27/61 (44%)
Frame = -2
Query: 270 FLYNYWNTIRIFPANFLTLRLPFLKRMXXXXXXXXXXXFLRNNLTKKLLFSSTQSTATCF 91
F +Y N ++ FP FLKR L++ + KK +T S+A+CF
Sbjct: 460 FYVDYNNGLKRFPKMSAMWFKEFLKREEEIEDSEEEEYVLKSTMNKKRFLLATGSSASCF 519
Query: 90 L 88
+
Sbjct: 520 I 520
>At3g19170.1 68416.m02434 peptidase M16 family protein / insulinase
family protein contains Pfam domain, PF05193: Peptidase
M16 inactive domain
Length = 1080
Score = 29.9 bits (64), Expect = 4.6
Identities = 19/57 (33%), Positives = 30/57 (52%), Gaps = 1/57 (1%)
Frame = +1
Query: 163 KMKFQYKEEHSFE-KRKTEGEKIRRKYPDRVPVIVEKAPKARLGDLDKKKYLVPSDL 330
K+K EE E R TE K++++ PD P + P LGD+ K+ VP+++
Sbjct: 586 KVKAAMTEEDLAELARATEELKLKQETPDP-PEALRCVPSLNLGDIPKEPTYVPTEV 641
>At3g47400.1 68416.m05154 pectinesterase family protein similar to
pectinesterase (EC 3.1.1.11) from Vitis vinifera
GI:15081598, Lycopersicon esculentum SP|Q43143
SP|P14280; contains Pfam profile PF01095 pectinesterase
Length = 594
Score = 29.5 bits (63), Expect = 6.1
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +1
Query: 853 PWPXPTXSPXXTSPLSPAGVIA 918
P P PT SP TSP SP V A
Sbjct: 50 PSPTPTSSPSTTSPPSPGPVAA 71
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 26,007,732
Number of Sequences: 28952
Number of extensions: 501846
Number of successful extensions: 1087
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 1036
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1084
length of database: 12,070,560
effective HSP length: 84
effective length of database: 9,638,592
effective search space used: 4077124416
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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