BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= 030723E4_H08_e352_16.seq (1512 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC4F10.07c |atg13|apg13, mug78|autophagy associated protein At... 31 0.31 SPBC216.07c |tor2|SPBC646.01c|phosphatidylinositol kinase Tor2|S... 31 0.55 SPAC29A4.11 |rga3||GTPase activating protein Rga3|Schizosaccharo... 28 3.9 SPAC16C9.06c |upf1||ATP-dependent RNA helicase Upf1|Schizosaccha... 27 8.9 SPAC1093.03 |||inositol polyphosphate phosphatase |Schizosacchar... 27 8.9 >SPAC4F10.07c |atg13|apg13, mug78|autophagy associated protein Atg13 |Schizosaccharomyces pombe|chr 1|||Manual Length = 758 Score = 31.5 bits (68), Expect = 0.31 Identities = 19/84 (22%), Positives = 36/84 (42%), Gaps = 1/84 (1%) Frame = +2 Query: 38 TSGIPRAAGNSARGSPLTHTLRTTRARPLVTYRGSRPPPPNTLQRAAPLXXXXXXXXXXV 217 T + GNS+ HTL T R+RP + + + P P ++ +A+ + Sbjct: 555 TEAVKHNLGNSSHSIMRHHTLGTLRSRPSFSEKSTFPAPLTSISQASTFQGDNRSPSTVI 614 Query: 218 -HSNGPAPPSSLFNGWCSLLHNKR 286 H+ P ++ + + LH+ R Sbjct: 615 PHTQTEVPSANDTSKQLASLHDMR 638 >SPBC216.07c |tor2|SPBC646.01c|phosphatidylinositol kinase Tor2|Schizosaccharomyces pombe|chr 2|||Manual Length = 2337 Score = 30.7 bits (66), Expect = 0.55 Identities = 16/43 (37%), Positives = 25/43 (58%) Frame = -3 Query: 412 LRVFEIKCSMYYLEEKNNNKKPRRTASLNTELVFGRGPL*TET 284 L F C + YLE NN+ + R+TAS+ +F R P+ ++T Sbjct: 476 LNEFVKDCVVVYLE--NNDPEVRKTASITCSQLFARDPILSQT 516 >SPAC29A4.11 |rga3||GTPase activating protein Rga3|Schizosaccharomyces pombe|chr 1|||Manual Length = 969 Score = 27.9 bits (59), Expect = 3.9 Identities = 17/55 (30%), Positives = 21/55 (38%) Frame = +3 Query: 120 PLSHTVVRDRRPPIHSNGRRPSAHAQTDAAPRFTPTGRRPPVHSSTGGVPYYTTN 284 P + DR PI NG S H T F+P+ R HS P T+ Sbjct: 323 PYKTMSLTDRAEPIVMNGHMRSLHNATSPFRPFSPSYRSSDTHSPRTRSPNVQTH 377 >SPAC16C9.06c |upf1||ATP-dependent RNA helicase Upf1|Schizosaccharomyces pombe|chr 1|||Manual Length = 925 Score = 26.6 bits (56), Expect = 8.9 Identities = 16/60 (26%), Positives = 27/60 (45%), Gaps = 4/60 (6%) Frame = +1 Query: 187 LTLKQTPLLGSLQRAGAPQFTLQRVVFPITQQTFPFI----GAPFQIPTQYSKRPCAAVF 354 L KQ L+G Q+ G + + ++Q F + +PF++ QY PC + F Sbjct: 580 LGAKQVVLVGDHQQLGPVVMNKKVALASLSQSLFERLIILGNSPFRLVVQYRMHPCLSEF 639 >SPAC1093.03 |||inositol polyphosphate phosphatase |Schizosaccharomyces pombe|chr 1|||Manual Length = 832 Score = 26.6 bits (56), Expect = 8.9 Identities = 11/24 (45%), Positives = 17/24 (70%) Frame = +3 Query: 240 PVHSSTGGVPYYTTNVSVYRGPLP 311 P+ S+T G+P YT+ V +RG +P Sbjct: 271 PISSTTPGIPCYTSYVQ-HRGSIP 293 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 4,313,312 Number of Sequences: 5004 Number of extensions: 78682 Number of successful extensions: 162 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 150 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 162 length of database: 2,362,478 effective HSP length: 76 effective length of database: 1,982,174 effective search space used: 846388298 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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