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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 030723E4_H08_e352_16.seq
         (1512 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC4F10.07c |atg13|apg13, mug78|autophagy associated protein At...    31   0.31 
SPBC216.07c |tor2|SPBC646.01c|phosphatidylinositol kinase Tor2|S...    31   0.55 
SPAC29A4.11 |rga3||GTPase activating protein Rga3|Schizosaccharo...    28   3.9  
SPAC16C9.06c |upf1||ATP-dependent RNA helicase Upf1|Schizosaccha...    27   8.9  
SPAC1093.03 |||inositol polyphosphate phosphatase |Schizosacchar...    27   8.9  

>SPAC4F10.07c |atg13|apg13, mug78|autophagy associated protein Atg13
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 758

 Score = 31.5 bits (68), Expect = 0.31
 Identities = 19/84 (22%), Positives = 36/84 (42%), Gaps = 1/84 (1%)
 Frame = +2

Query: 38  TSGIPRAAGNSARGSPLTHTLRTTRARPLVTYRGSRPPPPNTLQRAAPLXXXXXXXXXXV 217
           T  +    GNS+      HTL T R+RP  + + + P P  ++ +A+            +
Sbjct: 555 TEAVKHNLGNSSHSIMRHHTLGTLRSRPSFSEKSTFPAPLTSISQASTFQGDNRSPSTVI 614

Query: 218 -HSNGPAPPSSLFNGWCSLLHNKR 286
            H+    P ++  +   + LH+ R
Sbjct: 615 PHTQTEVPSANDTSKQLASLHDMR 638


>SPBC216.07c |tor2|SPBC646.01c|phosphatidylinositol kinase
           Tor2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 2337

 Score = 30.7 bits (66), Expect = 0.55
 Identities = 16/43 (37%), Positives = 25/43 (58%)
 Frame = -3

Query: 412 LRVFEIKCSMYYLEEKNNNKKPRRTASLNTELVFGRGPL*TET 284
           L  F   C + YLE  NN+ + R+TAS+    +F R P+ ++T
Sbjct: 476 LNEFVKDCVVVYLE--NNDPEVRKTASITCSQLFARDPILSQT 516


>SPAC29A4.11 |rga3||GTPase activating protein
           Rga3|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 969

 Score = 27.9 bits (59), Expect = 3.9
 Identities = 17/55 (30%), Positives = 21/55 (38%)
 Frame = +3

Query: 120 PLSHTVVRDRRPPIHSNGRRPSAHAQTDAAPRFTPTGRRPPVHSSTGGVPYYTTN 284
           P     + DR  PI  NG   S H  T     F+P+ R    HS     P   T+
Sbjct: 323 PYKTMSLTDRAEPIVMNGHMRSLHNATSPFRPFSPSYRSSDTHSPRTRSPNVQTH 377


>SPAC16C9.06c |upf1||ATP-dependent RNA helicase
           Upf1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 925

 Score = 26.6 bits (56), Expect = 8.9
 Identities = 16/60 (26%), Positives = 27/60 (45%), Gaps = 4/60 (6%)
 Frame = +1

Query: 187 LTLKQTPLLGSLQRAGAPQFTLQRVVFPITQQTFPFI----GAPFQIPTQYSKRPCAAVF 354
           L  KQ  L+G  Q+ G      +  +  ++Q  F  +     +PF++  QY   PC + F
Sbjct: 580 LGAKQVVLVGDHQQLGPVVMNKKVALASLSQSLFERLIILGNSPFRLVVQYRMHPCLSEF 639


>SPAC1093.03 |||inositol polyphosphate phosphatase
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 832

 Score = 26.6 bits (56), Expect = 8.9
 Identities = 11/24 (45%), Positives = 17/24 (70%)
 Frame = +3

Query: 240 PVHSSTGGVPYYTTNVSVYRGPLP 311
           P+ S+T G+P YT+ V  +RG +P
Sbjct: 271 PISSTTPGIPCYTSYVQ-HRGSIP 293


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 4,313,312
Number of Sequences: 5004
Number of extensions: 78682
Number of successful extensions: 162
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 150
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 162
length of database: 2,362,478
effective HSP length: 76
effective length of database: 1,982,174
effective search space used: 846388298
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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