Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030723E4_H07_e344_15.seq
(1463 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g18020.1 68415.m02094 60S ribosomal protein L8 (RPL8A) 246 2e-65
At4g36130.1 68417.m05142 60S ribosomal protein L8 (RPL8C) riboso... 241 6e-64
At3g51190.1 68416.m05604 60S ribosomal protein L8 (RPL8B) riboso... 236 2e-62
At4g14250.1 68417.m02198 UBX domain-containing protein low simil... 46 6e-05
At2g44065.2 68415.m05480 ribosomal protein L2 family protein sim... 46 8e-05
At2g44065.1 68415.m05479 ribosomal protein L2 family protein sim... 46 8e-05
At4g35110.2 68417.m04989 expressed protein 30 4.4
At4g35110.1 68417.m04988 expressed protein 30 4.4
>At2g18020.1 68415.m02094 60S ribosomal protein L8 (RPL8A)
Length = 258
Score = 246 bits (603), Expect = 2e-65
Identities = 112/158 (70%), Positives = 134/158 (84%)
Frame = +2
Query: 128 MGRVIRAQRKGAGSVFVSHTKKRKGAPKLRSLDYAERHGYIKGVVKDIIHDPGRGAPLAV 307
MGRVIRAQRKGAGSVF SHT RKG K RSLD+ ER+GY+KGVV +IIHDPGRGAPLA
Sbjct: 1 MGRVIRAQRKGAGSVFKSHTHHRKGPAKFRSLDFGERNGYLKGVVTEIIHDPGRGAPLAR 60
Query: 308 VHFRDPYKFKTRKELFIAPEGLYTGQFVYCGKKATLEVGNVMPVGAMPEGTIVCNLEEKM 487
V FR P++FK +KELF+A EG+YTGQF+YCGKKATL VGNV+P+ ++PEG +VCN+E +
Sbjct: 61 VTFRHPFRFKKQKELFVAAEGMYTGQFLYCGKKATLVVGNVLPLRSIPEGAVVCNVEHHV 120
Query: 488 GDRGRLARASGNFATVIGHNPDAKRTRVKLPSGAKKSV 601
GDRG LARASG++A VI HNPD+ TR+KLPSG+KK V
Sbjct: 121 GDRGVLARASGDYAIVIAHNPDSDTTRIKLPSGSKKIV 158
Score = 158 bits (383), Expect = 1e-38
Identities = 70/94 (74%), Positives = 79/94 (84%)
Frame = +3
Query: 591 KKVLPSSNRGMVGIVAGGGRIDKPILKAGRAYHKYKVKRNCWPYVRGVAMNPVEHPHGGG 770
KK++PS R M+G VAGGGR +KP+LKAG AYHKY+VKRN WP VRGVAMNPVEHPHGGG
Sbjct: 155 KKIVPSGCRAMIGQVAGGGRTEKPMLKAGNAYHKYRVKRNSWPKVRGVAMNPVEHPHGGG 214
Query: 771 NHQHIGKASTVKRGTSAGRKVGLIAARXTGXIRG 872
NHQHIG ASTV+R G+KVGLIAAR TG +RG
Sbjct: 215 NHQHIGHASTVRRDAPPGQKVGLIAARRTGRLRG 248
>At4g36130.1 68417.m05142 60S ribosomal protein L8 (RPL8C) ribosomal
protein L8, cytosolic, tomato, PIR1:R5TOL8
Length = 258
Score = 241 bits (591), Expect = 6e-64
Identities = 109/158 (68%), Positives = 132/158 (83%)
Frame = +2
Query: 128 MGRVIRAQRKGAGSVFVSHTKKRKGAPKLRSLDYAERHGYIKGVVKDIIHDPGRGAPLAV 307
MGRVIRAQRKGAGSVF SHT RKG K RSLD+ ER+GY+KGVV +IIHDPGRGAPLA
Sbjct: 1 MGRVIRAQRKGAGSVFKSHTHHRKGPAKFRSLDFGERNGYLKGVVTEIIHDPGRGAPLAR 60
Query: 308 VHFRDPYKFKTRKELFIAPEGLYTGQFVYCGKKATLEVGNVMPVGAMPEGTIVCNLEEKM 487
V FR P++FK +KELF+A EG+YTGQF+YCGKKATL VGNV+P+ ++PEG ++CN+E +
Sbjct: 61 VAFRHPFRFKKQKELFVAAEGMYTGQFLYCGKKATLVVGNVLPLRSIPEGAVICNVEHHV 120
Query: 488 GDRGRLARASGNFATVIGHNPDAKRTRVKLPSGAKKSV 601
GDRG ARASG++A VI HNPD +R+KLPSG+KK V
Sbjct: 121 GDRGVFARASGDYAIVIAHNPDNDTSRIKLPSGSKKIV 158
Score = 162 bits (394), Expect = 5e-40
Identities = 71/94 (75%), Positives = 80/94 (85%)
Frame = +3
Query: 591 KKVLPSSNRGMVGIVAGGGRIDKPILKAGRAYHKYKVKRNCWPYVRGVAMNPVEHPHGGG 770
KK++PS R M+G VAGGGR +KP+LKAG AYHKY+VKRNCWP VRGVAMNPVEHPHGGG
Sbjct: 155 KKIVPSGCRAMIGQVAGGGRTEKPMLKAGNAYHKYRVKRNCWPKVRGVAMNPVEHPHGGG 214
Query: 771 NHQHIGKASTVKRGTSAGRKVGLIAARXTGXIRG 872
NHQHIG ASTV+R G+KVGLIAAR TG +RG
Sbjct: 215 NHQHIGHASTVRRDAPPGKKVGLIAARRTGRLRG 248
>At3g51190.1 68416.m05604 60S ribosomal protein L8 (RPL8B) ribosomal
protein L8, cytosolic - Arabidopsis thaliana, PIR:T04582
Length = 260
Score = 236 bits (578), Expect = 2e-62
Identities = 107/157 (68%), Positives = 132/157 (84%), Gaps = 1/157 (0%)
Frame = +2
Query: 128 MGRVIRAQRKGA-GSVFVSHTKKRKGAPKLRSLDYAERHGYIKGVVKDIIHDPGRGAPLA 304
MGRVIRAQRKGA GSVF SHT RKG K RSLDY ER+GY+KG+V +IIHDPGRGAPLA
Sbjct: 1 MGRVIRAQRKGAAGSVFKSHTHHRKGPAKFRSLDYGERNGYLKGLVTEIIHDPGRGAPLA 60
Query: 305 VVHFRDPYKFKTRKELFIAPEGLYTGQFVYCGKKATLEVGNVMPVGAMPEGTIVCNLEEK 484
V FR P+++ +KELF+A EG+YTGQ++YCGKKA L VGNV+P+G++PEG ++CN+E
Sbjct: 61 RVAFRHPFRYMKQKELFVAAEGMYTGQYLYCGKKANLMVGNVLPLGSIPEGAVICNVELH 120
Query: 485 MGDRGRLARASGNFATVIGHNPDAKRTRVKLPSGAKK 595
+GDRG LARASG++A VI HNP++ TRVKLPSG+KK
Sbjct: 121 VGDRGALARASGDYAIVIAHNPESNTTRVKLPSGSKK 157
Score = 161 bits (392), Expect = 8e-40
Identities = 73/94 (77%), Positives = 78/94 (82%)
Frame = +3
Query: 591 KKVLPSSNRGMVGIVAGGGRIDKPILKAGRAYHKYKVKRNCWPYVRGVAMNPVEHPHGGG 770
KK+LPS+ R M+G VAGGGR +KP LKAG AYHKYK KRNCWP VRGVAMNPVEHPHGGG
Sbjct: 156 KKILPSACRAMIGQVAGGGRTEKPFLKAGNAYHKYKAKRNCWPVVRGVAMNPVEHPHGGG 215
Query: 771 NHQHIGKASTVKRGTSAGRKVGLIAARXTGXIRG 872
NHQHIG ASTV+R SAG KVG IAAR TG RG
Sbjct: 216 NHQHIGHASTVRRDKSAGAKVGQIAARRTGRRRG 249
>At4g14250.1 68417.m02198 UBX domain-containing protein low
similarity to 60S ribosomal protein L2 [Nicotiana
tabacum] GI:9230281; contains Pfam profile PF00789: UBX
domain
Length = 724
Score = 46.0 bits (104), Expect = 6e-05
Identities = 29/60 (48%), Positives = 34/60 (56%), Gaps = 1/60 (1%)
Frame = +3
Query: 591 KKVLPSSNRGMVGIVAGGGRIDKPILKAGRAYHKYKVKRNCWPYVRGVAM-NPVEHPHGG 767
KK + S R M+G +A G + K K +KRN W VRGVAM NPVEHPHGG
Sbjct: 389 KKTVLSGCRVMIGQIASSG-----LTK------KLMIKRNMWAKVRGVAMMNPVEHPHGG 437
>At2g44065.2 68415.m05480 ribosomal protein L2 family protein
similar to ribosomal protein L2 [Gossypium arboreum]
GI:17644114; contains Pfam profile PF03947: Ribosomal
Proteins L2, C-terminal domain
Length = 214
Score = 45.6 bits (103), Expect = 8e-05
Identities = 23/64 (35%), Positives = 36/64 (56%)
Frame = +2
Query: 419 VGNVMPVGAMPEGTIVCNLEEKMGDRGRLARASGNFATVIGHNPDAKRTRVKLPSGAKKS 598
+G+ MP+G M GTI+ N+E G ++ RA+G A ++ P + +KLPSG K
Sbjct: 59 IGSSMPLGMMRIGTIIHNIEMNPGQGAKMVRAAGTNAKIL-KEPAKGKCLIKLPSGDTKW 117
Query: 599 VTIK 610
+ K
Sbjct: 118 INAK 121
Score = 41.5 bits (93), Expect = 0.001
Identities = 23/52 (44%), Positives = 31/52 (59%)
Frame = +3
Query: 615 RGMVGIVAGGGRIDKPILKAGRAYHKYKVKRNCWPYVRGVAMNPVEHPHGGG 770
R +G V+ K + KAG++ ++ R P VRGVAMNP +HPHGGG
Sbjct: 123 RATIGTVSNPSHGTKKLYKAGQS--RWLGIR---PKVRGVAMNPCDHPHGGG 169
>At2g44065.1 68415.m05479 ribosomal protein L2 family protein
similar to ribosomal protein L2 [Gossypium arboreum]
GI:17644114; contains Pfam profile PF03947: Ribosomal
Proteins L2, C-terminal domain
Length = 214
Score = 45.6 bits (103), Expect = 8e-05
Identities = 23/64 (35%), Positives = 36/64 (56%)
Frame = +2
Query: 419 VGNVMPVGAMPEGTIVCNLEEKMGDRGRLARASGNFATVIGHNPDAKRTRVKLPSGAKKS 598
+G+ MP+G M GTI+ N+E G ++ RA+G A ++ P + +KLPSG K
Sbjct: 59 IGSSMPLGMMRIGTIIHNIEMNPGQGAKMVRAAGTNAKIL-KEPAKGKCLIKLPSGDTKW 117
Query: 599 VTIK 610
+ K
Sbjct: 118 INAK 121
Score = 41.5 bits (93), Expect = 0.001
Identities = 23/52 (44%), Positives = 31/52 (59%)
Frame = +3
Query: 615 RGMVGIVAGGGRIDKPILKAGRAYHKYKVKRNCWPYVRGVAMNPVEHPHGGG 770
R +G V+ K + KAG++ ++ R P VRGVAMNP +HPHGGG
Sbjct: 123 RATIGTVSNPSHGTKKLYKAGQS--RWLGIR---PKVRGVAMNPCDHPHGGG 169
>At4g35110.2 68417.m04989 expressed protein
Length = 386
Score = 29.9 bits (64), Expect = 4.4
Identities = 16/61 (26%), Positives = 29/61 (47%), Gaps = 1/61 (1%)
Frame = -3
Query: 735 QHHVHMASSCVSLCICDKHAQPSR-WACQYDHHPRQYQPCLCCLMVTLFLAPDGSFTRVR 559
++ V SS + + +KH ++ + D +Y CLC LM L P G ++V+
Sbjct: 216 KYRVRSGSSTILSAVIEKHGDIAQNCKLESDSMRSRYLECLCSLMQELRSTPVGQLSKVK 275
Query: 558 L 556
+
Sbjct: 276 V 276
>At4g35110.1 68417.m04988 expressed protein
Length = 386
Score = 29.9 bits (64), Expect = 4.4
Identities = 16/61 (26%), Positives = 29/61 (47%), Gaps = 1/61 (1%)
Frame = -3
Query: 735 QHHVHMASSCVSLCICDKHAQPSR-WACQYDHHPRQYQPCLCCLMVTLFLAPDGSFTRVR 559
++ V SS + + +KH ++ + D +Y CLC LM L P G ++V+
Sbjct: 216 KYRVRSGSSTILSAVIEKHGDIAQNCKLESDSMRSRYLECLCSLMQELRSTPVGQLSKVK 275
Query: 558 L 556
+
Sbjct: 276 V 276
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 25,026,346
Number of Sequences: 28952
Number of extensions: 523884
Number of successful extensions: 1249
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 1178
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1247
length of database: 12,070,560
effective HSP length: 84
effective length of database: 9,638,592
effective search space used: 3884352576
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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