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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 030723E4_H05_e328_15.seq
         (1464 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_52294| Best HMM Match : No HMM Matches (HMM E-Value=.)              83   7e-16
SB_43960| Best HMM Match : Toxin_19 (HMM E-Value=0.56)                 31   2.3  
SB_5592| Best HMM Match : No HMM Matches (HMM E-Value=.)               31   3.1  
SB_56370| Best HMM Match : No HMM Matches (HMM E-Value=.)              30   4.0  
SB_55739| Best HMM Match : Protamine_P2 (HMM E-Value=1.9)              29   7.1  
SB_1121| Best HMM Match : No HMM Matches (HMM E-Value=.)               29   9.3  
SB_9843| Best HMM Match : LIM (HMM E-Value=8.4e-07)                    29   9.3  

>SB_52294| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 394

 Score = 82.6 bits (195), Expect = 7e-16
 Identities = 52/139 (37%), Positives = 67/139 (48%), Gaps = 10/139 (7%)
 Frame = +3

Query: 51  AAGNSARGRPAYD-NRG--KREFDRRSGSDKTGVKPIDKREGAGPHNWGTLKDEIDE--- 212
           A G   RGR  +  NRG  KREF+RRSGSD++ VKP DKREG G  NWG   D  +E   
Sbjct: 151 ARGTGGRGRGGFGANRGGRKREFERRSGSDRSSVKPQDKREGGGQFNWGNPADTEEEGVV 210

Query: 213 INKXXXXXXXX----XXKAPEAGAGDGQQXXXXXXXXXXXXXXXXXXXXYKALRNAQRTM 380
            N+               +P+  A +GQ+                    +K L+N  R  
Sbjct: 211 YNQEKPDVEGSPEPGSPGSPKPDAEEGQEQNEEGDAEKEEEQKEMSLEEWKELQNKYRAK 270

Query: 381 PNYNLRKAGEGEDLSQWKN 437
            ++ LRK GEGE  +QWKN
Sbjct: 271 MSFELRKPGEGEKKNQWKN 289


>SB_43960| Best HMM Match : Toxin_19 (HMM E-Value=0.56)
          Length = 166

 Score = 31.1 bits (67), Expect = 2.3
 Identities = 20/52 (38%), Positives = 26/52 (50%), Gaps = 1/52 (1%)
 Frame = -1

Query: 162 HVYRLVSRQFC-PNRTCGRTRACLDCHKQAALVPNXLQPGGXH*XLERPPPR 10
           ++ R  SR  C P++ C +      C KQA +  N   PG     LERPPPR
Sbjct: 12  NIVRCQSRSQCGPHQDCNKMLGL--CLKQANVPSNSCSPGDPL-VLERPPPR 60


>SB_5592| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 1092

 Score = 30.7 bits (66), Expect = 3.1
 Identities = 12/35 (34%), Positives = 21/35 (60%)
 Frame = +1

Query: 307 HPLRKNHGNSPWMSIKLSEMHNVRCLTII*ERPVK 411
           HPL  +H N+P +++K    H+  C+    ERP++
Sbjct: 740 HPLAHDHRNNPRLAVKAQTYHHYLCIPA--ERPIE 772


>SB_56370| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 548

 Score = 30.3 bits (65), Expect = 4.0
 Identities = 13/38 (34%), Positives = 18/38 (47%), Gaps = 4/38 (10%)
 Frame = +2

Query: 527 CGSSEACVGNRVHVQRSSTSWRNR----RAWQRPWART 628
           C   EA   N+  ++ S T W  +    R W+ PW RT
Sbjct: 265 CNKEEAACANKTSMEGSMTIWLPQGWALRKWRHPWQRT 302


>SB_55739| Best HMM Match : Protamine_P2 (HMM E-Value=1.9)
          Length = 678

 Score = 29.5 bits (63), Expect = 7.1
 Identities = 20/58 (34%), Positives = 26/58 (44%)
 Frame = +3

Query: 36  SGIPRAAGNSARGRPAYDNRGKREFDRRSGSDKTGVKPIDKREGAGPHNWGTLKDEID 209
           SGI        RG   YD R KR    R   D+ G    D+R+  G  + G L+D+ D
Sbjct: 192 SGIRIRVRKKERGGKEYDTRDKRSHRGRKWRDERGNTSRDQRD-EGLSDDGKLRDDDD 248


>SB_1121| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 219

 Score = 29.1 bits (62), Expect = 9.3
 Identities = 16/56 (28%), Positives = 22/56 (39%)
 Frame = -1

Query: 687 HSSALQDVPPAGALHAFPDRVRAHGLCHARLFLHDVLDR*T*TRFPTHASDDPHVE 520
           H S      P   LH  PD +  H   ++ L +HD +   T    P H  D  H +
Sbjct: 136 HDSIPTPTTPYSPLH-IPDSISTHTTPYSPLHIHDSISTPTTPYSPLHIHDSIHTQ 190


>SB_9843| Best HMM Match : LIM (HMM E-Value=8.4e-07)
          Length = 2128

 Score = 29.1 bits (62), Expect = 9.3
 Identities = 18/46 (39%), Positives = 24/46 (52%), Gaps = 8/46 (17%)
 Frame = +3

Query: 69   RGRPAYDNRGKR---EFDRRSGS-----DKTGVKPIDKREGAGPHN 182
            R R  YD RGKR   E+DRR        D+ G +P D+ +  GP +
Sbjct: 1766 RPRDEYDRRGKRPRDEYDRRGERPRDEYDRRGERPRDEYDRRGPRD 1811


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 28,125,456
Number of Sequences: 59808
Number of extensions: 504266
Number of successful extensions: 1159
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 1041
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1154
length of database: 16,821,457
effective HSP length: 85
effective length of database: 11,737,777
effective search space used: 4718586354
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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