Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030723E4_H03_e312_15.seq
(1528 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g64330.1 68414.m07290 myosin heavy chain-related similar to m... 40 0.003
At1g68790.1 68414.m07863 expressed protein 38 0.017
At5g52550.1 68418.m06525 expressed protein 37 0.031
At4g21270.1 68417.m03074 kinesin-like protein A (KATA) 36 0.093
At3g10880.1 68416.m01310 hypothetical protein 33 0.66
At4g05190.1 68417.m00781 kinesin-like protein A, putative kinesi... 32 0.87
At1g64880.1 68414.m07355 ribosomal protein S5 family protein con... 32 0.87
At5g43310.1 68418.m05293 COP1-interacting protein-related contai... 30 3.5
At5g35520.1 68418.m04224 kinetochore protein-related contains Pf... 30 3.5
At4g12770.1 68417.m02004 auxilin-related low similarity to SP|Q2... 30 3.5
At3g49305.1 68416.m05389 hypothetical protein contains proline-r... 30 4.6
At1g76810.1 68414.m08938 eukaryotic translation initiation facto... 30 4.6
At5g20960.2 68418.m02492 aldehyde oxidase 1 (AAO1) identical to ... 29 8.1
At5g20960.1 68418.m02491 aldehyde oxidase 1 (AAO1) identical to ... 29 8.1
At2g44790.1 68415.m05574 uclacyanin II strong similarity to ucla... 29 8.1
At1g67230.1 68414.m07652 expressed protein 29 8.1
>At1g64330.1 68414.m07290 myosin heavy chain-related similar to
myosin heavy chain (GI:1850913) [Entamoeba histolytica];
similar to Intracellular protein transport protein USO1
(Swiss-Prot:P25386) [Saccharomyces cerevisiae]
Length = 555
Score = 40.3 bits (90), Expect = 0.003
Identities = 43/183 (23%), Positives = 81/183 (44%), Gaps = 1/183 (0%)
Frame = +1
Query: 301 TMEESSFTIPADDNGDTELDPRIQVELEKLNAATDEINKLELELDESMKTFHLLLKETSR 480
T E + G+TE D +KL E + LE EL K L+E +R
Sbjct: 180 TSENKELNEKLEVAGETESDLN-----QKLEDVKKERDGLEAELASKAKDHESTLEEVNR 234
Query: 481 RLQALSKRLGACVEKSRPYYDAVGVAANARSECQRAAVQFQRA-SELHAAAKETVTLAEQ 657
LQ A +E+ + A+ N ++ Q+A ++ + A + L K+ L E+
Sbjct: 235 -LQGQKNETEAELEREKQEKPAL---LNQINDVQKALLEQEAAYNTLSQEHKQINGLFEE 290
Query: 658 RFVSKQDEWQFDNNWQEVLNHAIIKXMDAEKRKAESGREHQKKATAYIAAEKKVTTLEDD 837
R + + +E+L + K + E+R E+G++ + +A + E+ V +L ++
Sbjct: 291 REATIKKLTDDYKQAREMLEEYMSKMEETERRMQETGKDVASRESAIVDLEETVESLRNE 350
Query: 838 LKR 846
++R
Sbjct: 351 VER 353
>At1g68790.1 68414.m07863 expressed protein
Length = 1085
Score = 37.9 bits (84), Expect = 0.017
Identities = 41/177 (23%), Positives = 80/177 (45%), Gaps = 8/177 (4%)
Frame = +1
Query: 346 DTELDPRIQVELEKLNAATDEINKLELELDESMKTFHLLLKETSRRLQALSKRLGACVEK 525
+ +LD R++ EK A E KL +E + ++ KE R+L+ + +G K
Sbjct: 421 EKDLDARLKTVKEKEKALKAEEKKLHMENERLLED-----KECLRKLKDEIEEIGTETTK 475
Query: 526 S----RPYYDAVGVAANARSECQRAAVQFQRASELHAAAKETVTLAEQRFVSKQDEWQFD 693
R ++++ + R E R +Q + ++ +E L ++R KQD+ +F+
Sbjct: 476 QESRIREEHESLRITKEERVEFLR--LQSELKQQIDKVKQEEELLLKEREELKQDKERFE 533
Query: 694 NNWQEVLNHAIIKXMDAEKRKAESGREHQKKATAYIAAEKKV----TTLEDDLKRSI 852
W E L+ K + + + E E++K I+ + ++ T D+LKR +
Sbjct: 534 KEW-EALDK---KRANITREQNEVAEENEKLRNLQISEKHRLKREEMTSRDNLKREL 586
>At5g52550.1 68418.m06525 expressed protein
Length = 360
Score = 37.1 bits (82), Expect = 0.031
Identities = 31/120 (25%), Positives = 59/120 (49%), Gaps = 5/120 (4%)
Frame = +1
Query: 334 DDNGDTELDPRIQVELEKLNAATDEINKLELELDESMKTFHLLLKETSRRLQALSKRL-- 507
+D+ D + + ELE++ A ++ N+LE + S L K+ R+L+ KRL
Sbjct: 72 EDSADAAKKKQERDELERIKQAENKKNRLEKSIATSAAIMAELEKKKLRKLEE-QKRLAE 130
Query: 508 -GACV--EKSRPYYDAVGVAANARSECQRAAVQFQRASELHAAAKETVTLAEQRFVSKQD 678
GA + +K R A+ A R+E ++ Q ++ +L AA +E A ++ +++
Sbjct: 131 EGAAIAEKKKRRLEKAIATTAAIRAELEKKK-QMKKEGQLDAAVEEDSAYAAKKKQEREE 189
>At4g21270.1 68417.m03074 kinesin-like protein A (KATA)
Length = 793
Score = 35.5 bits (78), Expect = 0.093
Identities = 46/167 (27%), Positives = 76/167 (45%), Gaps = 10/167 (5%)
Frame = +1
Query: 376 ELEKLNAATDEINKLELELDESMKTFHLLLKETSRRLQALSKRLGACVEKSRPYYDA-VG 552
E K++ DEI L L E K K ++ + KRL CV+ + + V
Sbjct: 62 ECGKVDFTKDEILAL---LSERAKAGKFDTKAKIEQMTDIIKRLKVCVKWFQQADETHVQ 118
Query: 553 VAANARSECQRAAVQFQRASELHAAAKE-----TVTLAEQRFVSKQDEW-QFDNNWQEVL 714
N + + + ++ EL A KE T++ E+ VS ++ + +++ Q+ +
Sbjct: 119 EKENLKVSLESSEQKYNH-KELEARTKEEELQATISKLEENVVSLHEKLAKEESSTQDAI 177
Query: 715 N-HAIIKX--MDAEKRKAESGREHQKKATAYIAAEKKVTTLEDDLKR 846
H K + AEK +A G E K +AA++KVT+LED KR
Sbjct: 178 ECHRREKEARVAAEKVQASLGEELDKVKEEKMAAKQKVTSLEDMYKR 224
>At3g10880.1 68416.m01310 hypothetical protein
Length = 278
Score = 32.7 bits (71), Expect = 0.66
Identities = 28/90 (31%), Positives = 47/90 (52%), Gaps = 2/90 (2%)
Frame = +1
Query: 352 ELDPRIQVELE-KLNAATDEINKLELELDESMKTFHLLLKETSRRLQALSKRLGACVEKS 528
E + R V+LE KL+ + +I KLE ELDE + + E S+ ++ LS+ EKS
Sbjct: 124 ETEKRYVVDLESKLSDSVYKIEKLESELDEVKECLGVSEAEVSKLMEMLSECKN---EKS 180
Query: 529 RPYYD-AVGVAANARSECQRAAVQFQRASE 615
+ D A + + R+E + +Q ++ E
Sbjct: 181 KLQTDNADDLLDSLRAELRSREIQIEQMEE 210
>At4g05190.1 68417.m00781 kinesin-like protein A, putative kinesin
like protein A, Arabidopsis thaliana, gb:Q07970
Length = 790
Score = 32.3 bits (70), Expect = 0.87
Identities = 44/167 (26%), Positives = 74/167 (44%), Gaps = 17/167 (10%)
Frame = +1
Query: 397 ATDEINKLELELDESMKTFHLLLK----ETSRRLQALS---KRLGACVEKSRPYYDA-VG 552
+T+E K+E DE + + K +T +++ ++ K+L CV + + V
Sbjct: 56 STEECGKVEFTKDEVLALLNERAKAGKFDTKGKIEQMTDIIKKLKVCVRWYQQVDETHVQ 115
Query: 553 VAANARSECQRAAVQFQRASELHAAAKE-----TVTLAEQRFVSKQDEWQFD--NNWQEV 711
N S Q A ++ EL A KE T+T ++ S Q++ + + +
Sbjct: 116 DKENLSSSLQSAEKRYSD-KELDAKTKEEELRATITEMKENIESLQEKLSKEKLSKLDAI 174
Query: 712 LNHAIIKX--MDAEKRKAESGREHQKKATAYIAAEKKVTTLEDDLKR 846
NH K + AEK + E K +AA++KVT+LED KR
Sbjct: 175 ENHRREKDCRVVAEKLQVSLREELDKVKEEKMAAKQKVTSLEDMYKR 221
>At1g64880.1 68414.m07355 ribosomal protein S5 family protein
contains similarity to 30S ribosomal protein S5
GI:6969105 from [Campylobacter jejuni]
Length = 515
Score = 32.3 bits (70), Expect = 0.87
Identities = 26/73 (35%), Positives = 35/73 (47%), Gaps = 4/73 (5%)
Frame = +1
Query: 334 DDNGDTELDP---RIQVELEKLNAATDEINKLELELDESMKTFHLLLKETSRRLQALSKR 504
DD+ + E D R + LEKLNA KLE++L E TF K ++ L++
Sbjct: 285 DDDDEEEFDDMKERDDILLEKLNAID---KKLEIKLSELDHTFGKKGKRLEEEIRELAED 341
Query: 505 LGACVEKSR-PYY 540
A EK R P Y
Sbjct: 342 RNALTEKKRQPLY 354
>At5g43310.1 68418.m05293 COP1-interacting protein-related contains
similarity to COP1-Interacting Protein 7 (CIP7)
[Arabidopsis thaliana] GI:3327868
Length = 1237
Score = 30.3 bits (65), Expect = 3.5
Identities = 19/67 (28%), Positives = 28/67 (41%)
Frame = +1
Query: 700 WQEVLNHAIIKXMDAEKRKAESGREHQKKATAYIAAEKKVTTLEDDLKRSIXNRASTSXK 879
W EVLN + K + + R ++ + + EK + E D NR STS
Sbjct: 134 WSEVLNGILYKQVKDDHRAKKADSAEGSRPLLQLNEEKAIVLYEPDSHPKQANR-STSSD 192
Query: 880 EALRRTP 900
E + TP
Sbjct: 193 ENSKYTP 199
Score = 30.3 bits (65), Expect = 3.5
Identities = 14/41 (34%), Positives = 25/41 (60%)
Frame = +1
Query: 712 LNHAIIKXMDAEKRKAESGREHQKKATAYIAAEKKVTTLED 834
L A +K MD K+K E+G+ + +AT +A + ++T+ D
Sbjct: 278 LMDACVKFMDLWKKKHETGQWVEIEATEVMATQPNISTMND 318
>At5g35520.1 68418.m04224 kinetochore protein-related contains Pfam
PF05859: Mis12 protein
Length = 285
Score = 30.3 bits (65), Expect = 3.5
Identities = 25/104 (24%), Positives = 45/104 (43%), Gaps = 2/104 (1%)
Frame = +1
Query: 310 ESSFTIPADDNGDTELDPRIQVELEKLNAATDEINKLELELDESMKTFHLLLKETSRRLQ 489
E S ++ D D ELD + +KLN + K +ELD ++ R L+
Sbjct: 103 EESSSVHQDGLYDLELDAELDSLRDKLNV----VGKRSVELDSELQALERSSVSRERSLR 158
Query: 490 ALSKRLGACVEKSRP--YYDAVGVAANARSECQRAAVQFQRASE 615
+++ L E S + + +A+ R+ +R + +ASE
Sbjct: 159 LVNEALELYDESSMDEIFKEMTKMASELRASVERLKTRRMKASE 202
>At4g12770.1 68417.m02004 auxilin-related low similarity to
SP|Q27974 Auxilin {Bos taurus}
Length = 909
Score = 30.3 bits (65), Expect = 3.5
Identities = 26/96 (27%), Positives = 40/96 (41%)
Frame = +1
Query: 370 QVELEKLNAATDEINKLELELDESMKTFHLLLKETSRRLQALSKRLGACVEKSRPYYDAV 549
+ E+EK A E + E + E + LL ++ R ++ A ++ AV
Sbjct: 517 EAEMEKTQAREREEREREQKRIERERE-RLLARQAVERATREARERAATEAHAKVQRAAV 575
Query: 550 GVAANARSECQRAAVQFQRASELHAAAKETVTLAEQ 657
G +AR +RAAVQ A AA AE+
Sbjct: 576 GKVTDARERAERAAVQRAHAEARERAAAGAREKAEK 611
>At3g49305.1 68416.m05389 hypothetical protein contains proline-rich
extensin domains, INTERPRO:IPR002965
Length = 78
Score = 29.9 bits (64), Expect = 4.6
Identities = 14/43 (32%), Positives = 19/43 (44%), Gaps = 2/43 (4%)
Frame = +2
Query: 959 YSNRLSXRGIXXGSPETRGXPREGPXPV--VXKPGXGXRXPPP 1081
++N+ S + P P GP P+ KPG G PPP
Sbjct: 32 HTNQYSLNNLEESIPTIMDYPEPGPNPIHDPTKPGYGRPPPPP 74
>At1g76810.1 68414.m08938 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to IF2
protein [Drosophila melanogaster] GI:7108770; contains
Pfam profile PF03144: Elongation factor Tu domain 2
Length = 1294
Score = 29.9 bits (64), Expect = 4.6
Identities = 15/48 (31%), Positives = 24/48 (50%)
Frame = +1
Query: 742 AEKRKAESGREHQKKATAYIAAEKKVTTLEDDLKRSIXNRASTSXKEA 885
A+K+K + +E +KKA A AA V E+ + S+ K+A
Sbjct: 353 AKKKKKKKEKEKEKKAAAAAAATSSVEVKEEKQEESVTEPLQPKKKDA 400
>At5g20960.2 68418.m02492 aldehyde oxidase 1 (AAO1) identical to
aldehyde oxidase AAO1 from Arabidopsis thaliana
[gi:3172023] isoform contains a GA-donor splice site at
intron 10
Length = 1368
Score = 29.1 bits (62), Expect = 8.1
Identities = 15/54 (27%), Positives = 26/54 (48%)
Frame = +1
Query: 295 SSTMEESSFTIPADDNGDTELDPRIQVELEKLNAATDEINKLELELDESMKTFH 456
++T+ ++ P D G + I+ L+ DEI K+ L ES++ FH
Sbjct: 936 TNTVSRTALRAPGDVQGSYIGEAIIEKVASYLSVDVDEIRKVNLHTYESLRLFH 989
>At5g20960.1 68418.m02491 aldehyde oxidase 1 (AAO1) identical to
aldehyde oxidase AAO1 from Arabidopsis thaliana
[gi:3172023] isoform contains a GA-donor splice site at
intron 10
Length = 1368
Score = 29.1 bits (62), Expect = 8.1
Identities = 15/54 (27%), Positives = 26/54 (48%)
Frame = +1
Query: 295 SSTMEESSFTIPADDNGDTELDPRIQVELEKLNAATDEINKLELELDESMKTFH 456
++T+ ++ P D G + I+ L+ DEI K+ L ES++ FH
Sbjct: 936 TNTVSRTALRAPGDVQGSYIGEAIIEKVASYLSVDVDEIRKVNLHTYESLRLFH 989
>At2g44790.1 68415.m05574 uclacyanin II strong similarity to
uclacyanin II GI:3399769 from [Arabidopsis thaliana];
contains Pfam profile PF02298: Plastocyanin-like domain;
identical to cDNA uclacyanin II GI:3399768
Length = 202
Score = 29.1 bits (62), Expect = 8.1
Identities = 13/31 (41%), Positives = 14/31 (45%), Gaps = 2/31 (6%)
Frame = +2
Query: 995 GSPETRGXPREG--PXPVVXKPGXGXRXPPP 1081
G+P T P G P P PG G PPP
Sbjct: 143 GTPTTPESPPSGGSPTPTTPTPGAGSTSPPP 173
>At1g67230.1 68414.m07652 expressed protein
Length = 1132
Score = 29.1 bits (62), Expect = 8.1
Identities = 37/181 (20%), Positives = 76/181 (41%), Gaps = 11/181 (6%)
Frame = +1
Query: 355 LDPRIQVELEK-LNAATDEINKLELELDESMKTFHLLLKETSRRLQALSKRLGAC----V 519
++ + ++LEK L E +++ D + + L++ + + +L A
Sbjct: 125 IEKQCALDLEKALKELRAENAEIKFTADSKLTEANALVRSVEEKSLEVEAKLRAVDAKLA 184
Query: 520 EKSRPYYDAVGVAANARSECQRAAVQFQRASELHAAAKETVTLAEQRFVSKQDEWQFDNN 699
E SR D A E + +++Q +R S + + TL++QR ++ E +
Sbjct: 185 EVSRKSSDVERKAKEV--EARESSLQRERFSYIAEREADEATLSKQREDLREWERKLQEG 242
Query: 700 WQEVLNHAII------KXMDAEKRKAESGREHQKKATAYIAAEKKVTTLEDDLKRSIXNR 861
+ V +I + +++K + G+E ++ AA V LEDD+ I +
Sbjct: 243 EERVAKSQMIVKQREDRANESDKIIKQKGKELEEAQKKIDAANLAVKKLEDDVSSRIKDL 302
Query: 862 A 864
A
Sbjct: 303 A 303
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 23,186,440
Number of Sequences: 28952
Number of extensions: 413802
Number of successful extensions: 999
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 955
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 996
length of database: 12,070,560
effective HSP length: 84
effective length of database: 9,638,592
effective search space used: 4086763008
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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