BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= 030723E4_H01_e296_15.seq (1581 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyce... 34 0.062 SPAC4H3.14c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 33 0.11 SPAC4F10.06 |||BUD22 family protein|Schizosaccharomyces pombe|ch... 31 0.58 SPAC823.05c |tlg2||SNARE Tlg2|Schizosaccharomyces pombe|chr 1|||... 30 1.0 SPBC24C6.10c |||conserved eukaryotic protein|Schizosaccharomyces... 29 1.3 SPAC27D7.02c |||GRIP domain protein|Schizosaccharomyces pombe|ch... 29 1.8 SPBC16G5.12c |top3||DNA topoisomerase III|Schizosaccharomyces po... 29 1.8 SPAPB18E9.02c |ppk18||serine/threonine protein kinase Ppk18 |Sch... 29 2.3 SPBC3E7.11c |||DNAJ protein Caj1/Djp1-type|Schizosaccharomyces p... 28 3.1 SPAC806.02c |||Par A family ATPase iron cluster assembly protein... 28 4.1 SPAC23C11.04c |pnk1||DNA kinase/phosphatase Pnk1|Schizosaccharom... 27 5.4 SPCC364.01 |||sequence orphan|Schizosaccharomyces pombe|chr 3|||... 27 5.4 SPCC162.08c |nup211||nuclear pore complex associated protein|Sch... 27 9.4 >SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyces pombe|chr 1|||Manual Length = 1957 Score = 33.9 bits (74), Expect = 0.062 Identities = 24/128 (18%), Positives = 52/128 (40%) Frame = +1 Query: 70 IRHEXADKLSTRVQGTAKQIEDLEKMAADNMQLTRDAKMKVGQANTDAREAEKQVSKGLE 249 I++E +KL G+ K + M +++ + TDA + + Sbjct: 343 IKNEKLEKLLRNTIGSLKDSRTSNSQLEEEMVELKESNRTIHSQLTDAESKLSSFEQENK 402 Query: 250 DLKIIMDELQNLPTLDDAALDRLQDSLDTAEKALKTVDLDGKIKSLTEAKNDHQRWMKEY 429 LK +DE QN + D + ++ L+ A +L GK+ + ++ + +K++ Sbjct: 403 SLKGSIDEYQNNLSSKDKMVKQVSSQLEEARSSL--AHATGKLAEINSERDFQNKKIKDF 460 Query: 430 QDEHDHLK 453 + L+ Sbjct: 461 EKIEQDLR 468 Score = 28.7 bits (61), Expect = 2.3 Identities = 23/123 (18%), Positives = 60/123 (48%), Gaps = 1/123 (0%) Frame = +1 Query: 121 KQIEDLEKMAADNMQLTRDAKMKVGQANTDAREAEKQVSKGLEDLKIIMDELQNLPTLDD 300 K ED++++ A+ LT+D+K + Q+ T + + +S +L+ D+ N+ + ++ Sbjct: 756 KSSEDVKRLTANVETLTQDSK-AMKQSFTSLVNSYQSISNLYHELR---DDHVNMQSQNN 811 Query: 301 AALD-RLQDSLDTAEKALKTVDLDGKIKSLTEAKNDHQRWMKEYQDEHDHLKIEVENIKD 477 L+ + D + + L ++ L + + + E ++ + L ++++N++ Sbjct: 812 TLLESESKLKTDCENLTQQNMTLIDNVQKLMHKHVNQESKVSELKEVNGKLSLDLKNLRS 871 Query: 478 ILN 486 LN Sbjct: 872 SLN 874 Score = 27.5 bits (58), Expect = 5.4 Identities = 20/82 (24%), Positives = 42/82 (51%), Gaps = 6/82 (7%) Frame = +1 Query: 118 AKQIEDLEKMAADNMQLTRD-----AKMKVGQANTDAREAEKQ-VSKGLEDLKIIMDELQ 279 A ++DL++ + QLT + ++ +N +A E+ ++ L D+K + + Sbjct: 1133 ANFVDDLKEKSDALEQLTNEKNELIVSLEQSNSNNEALVEERSDLANRLSDMKKSLSDSD 1192 Query: 280 NLPTLDDAALDRLQDSLDTAEK 345 N+ ++ + L R+ D LDT +K Sbjct: 1193 NVISVIRSDLVRVNDELDTLKK 1214 >SPAC4H3.14c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 345 Score = 33.1 bits (72), Expect = 0.11 Identities = 37/149 (24%), Positives = 70/149 (46%), Gaps = 9/149 (6%) Frame = +1 Query: 73 RHEXADKLSTRVQGT-AKQIEDL-EKMAADNMQLTRDAKMKVGQANTDAREAEKQVSKGL 246 R + A K ++Q +QI DL E+ +++ R + + +AN++ + EKQ Sbjct: 129 RKQRAAKEEQKIQEEFERQITDLLEEQQQLKLEIER-LEAETERANSETEQYEKQKEALE 187 Query: 247 EDLKIIMDE-LQNLPTLDDAALDRLQDSLDTAEKALKTVDLDGKI------KSLTEAKND 405 E+ + + +E L++ P L DA + LQD+ + E+ L D KI S+ + ++ Sbjct: 188 EEYEELRNECLKHDPQL-DAEIRTLQDTFEEVERTLTKQVSDAKIADKPLKDSMFNSNSE 246 Query: 406 HQRWMKEYQDEHDHLKIEVENIKDILNQL 492 ++ M + I E I+ + QL Sbjct: 247 KEKIMHALEKAEKDADIYSEFIQQYMEQL 275 >SPAC4F10.06 |||BUD22 family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 388 Score = 30.7 bits (66), Expect = 0.58 Identities = 33/129 (25%), Positives = 51/129 (39%), Gaps = 11/129 (8%) Frame = +1 Query: 97 STRVQGTAKQIEDLEKMAADNMQLTRDAK-MKVGQANTDAREAEKQVSKGLEDLKIIMDE 273 +T VQ + + M DN + K M N +E+ Q + + II DE Sbjct: 191 NTHVQNSTDGVNQDTGMILDNTEDKEINKSMSYSMKNEGVKESSLQNATLINRKSIIDDE 250 Query: 274 L----------QNLPTLDDAALDRLQDSLDTAEKALKTVDLDGKIKSLTEAKNDHQRWMK 423 + NLP L LD ++ + EK L+ VD+ K K+ + W K Sbjct: 251 MLEIPLGKHNNTNLPALTAGFLDPVESDDEFVEKELEEVDIP-KRKNRRGQRARQAIWEK 309 Query: 424 EYQDEHDHL 450 +Y +HL Sbjct: 310 KYGKGANHL 318 >SPAC823.05c |tlg2||SNARE Tlg2|Schizosaccharomyces pombe|chr 1|||Manual Length = 301 Score = 29.9 bits (64), Expect = 1.0 Identities = 17/60 (28%), Positives = 29/60 (48%), Gaps = 1/60 (1%) Frame = +1 Query: 64 QXIRHEXA-DKLSTRVQGTAKQIEDLEKMAADNMQLTRDAKMKVGQANTDAREAEKQVSK 240 Q IRHE A K++ + A+ +DL+ + + L + Q A+ AEK++ K Sbjct: 208 QAIRHERAVAKIAEGIIELAQMFQDLQVLVIEQGALVDRIDFNIEQTQVHAKSAEKELIK 267 >SPBC24C6.10c |||conserved eukaryotic protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 374 Score = 29.5 bits (63), Expect = 1.3 Identities = 13/28 (46%), Positives = 20/28 (71%) Frame = -2 Query: 776 LNIFYNCLFFCKDQVSVNFKITQMFSTE 693 L+IFY+C FF K+Q ++ F I +F +E Sbjct: 79 LSIFYDCFFFLKEQQTLKF-IIIVFQSE 105 >SPAC27D7.02c |||GRIP domain protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 750 Score = 29.1 bits (62), Expect = 1.8 Identities = 28/142 (19%), Positives = 65/142 (45%), Gaps = 4/142 (2%) Frame = +1 Query: 79 EXADKLSTRVQGTAKQIEDLEKMAAD---NMQLTRDAKMKVGQANTDAREAE-KQVSKGL 246 + + KL V+ + +DL++ + N+ + ++K +++ +AE + + Sbjct: 530 QDSQKLRLLVEQLELERQDLKQAGENHYSNLSSDYETQIKSLESSLTNSQAECVSFQEKI 589 Query: 247 EDLKIIMDELQNLPTLDDAALDRLQDSLDTAEKALKTVDLDGKIKSLTEAKNDHQRWMKE 426 +L +DEL+ + L S + + ++V+ D + SL KN++Q +K Sbjct: 590 NELNSQIDELKLKLNEANKKYQELAISFENSNVKTQSVEPDNGL-SLEALKNENQTLLKN 648 Query: 427 YQDEHDHLKIEVENIKDILNQL 492 +D + ++ K++ NQL Sbjct: 649 LEDSTARYEHLQKSFKNVFNQL 670 >SPBC16G5.12c |top3||DNA topoisomerase III|Schizosaccharomyces pombe|chr 2|||Manual Length = 622 Score = 29.1 bits (62), Expect = 1.8 Identities = 24/89 (26%), Positives = 39/89 (43%) Frame = +1 Query: 55 PGLQXIRHEXADKLSTRVQGTAKQIEDLEKMAADNMQLTRDAKMKVGQANTDAREAEKQV 234 P +Q IR + + + + AK+ D+ K AAD + + ++G T + + Q Sbjct: 138 PSIQVIRADFNNLERSHIISAAKRPRDVSKNAADAVDARIELDFRLGAIFTRLQTIQLQK 197 Query: 235 SKGLEDLKIIMDELQNLPTLDDAALDRLQ 321 S + KII PTL +DR Q Sbjct: 198 SFDILQNKIISYGPCQFPTL-GFVVDRWQ 225 >SPAPB18E9.02c |ppk18||serine/threonine protein kinase Ppk18 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1316 Score = 28.7 bits (61), Expect = 2.3 Identities = 21/63 (33%), Positives = 26/63 (41%), Gaps = 4/63 (6%) Frame = +3 Query: 117 SETNRRFGENGGR*YAAYPRRQDEGGS----SEH*RKGGRETSIQRSRRFKDYNGRTPEL 284 +E+N N G + E GS S H R R TS +RSRRF + N E Sbjct: 968 AESNMSSSVNSGEEVSKDNNVSQERGSQFLRSSHGRSRERSTSARRSRRFSEANSEFDEF 1027 Query: 285 AYF 293 F Sbjct: 1028 GPF 1030 >SPBC3E7.11c |||DNAJ protein Caj1/Djp1-type|Schizosaccharomyces pombe|chr 2|||Manual Length = 355 Score = 28.3 bits (60), Expect = 3.1 Identities = 11/36 (30%), Positives = 19/36 (52%) Frame = +1 Query: 331 DTAEKALKTVDLDGKIKSLTEAKNDHQRWMKEYQDE 438 DT + VD+ +L +A+ ++W KE +DE Sbjct: 240 DTWNTVVSAVDVQSSAAALAKAEEGEEQWSKEKRDE 275 >SPAC806.02c |||Par A family ATPase iron cluster assembly protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 608 Score = 27.9 bits (59), Expect = 4.1 Identities = 11/36 (30%), Positives = 18/36 (50%) Frame = +1 Query: 367 DGKIKSLTEAKNDHQRWMKEYQDEHDHLKIEVENIK 474 DGKI K+D W Y+ +H H E+ +++ Sbjct: 551 DGKIVVYQREKHDEALWHVAYEQDHAHGVYEINSLE 586 >SPAC23C11.04c |pnk1||DNA kinase/phosphatase Pnk1|Schizosaccharomyces pombe|chr 1|||Manual Length = 421 Score = 27.5 bits (58), Expect = 5.4 Identities = 10/33 (30%), Positives = 17/33 (51%) Frame = -3 Query: 439 VRLDIPSSIFGGHSWLPSNS*FYHPNRQFLTPS 341 ++ + P F GHS++P N +HP + S Sbjct: 211 IKFETPEQFFLGHSFVPPNFESFHPKNYLVRNS 243 >SPCC364.01 |||sequence orphan|Schizosaccharomyces pombe|chr 3|||Manual Length = 320 Score = 27.5 bits (58), Expect = 5.4 Identities = 16/49 (32%), Positives = 27/49 (55%), Gaps = 1/49 (2%) Frame = +1 Query: 220 AEKQVSKGLEDL-KIIMDELQNLPTLDDAALDRLQDSLDTAEKALKTVD 363 AEK ++ L+ +I+ + +N L D +L RL SLD + + T+D Sbjct: 35 AEKLKARKLQKAAEIVQKQKENRKILKDVSLKRLSKSLDRSLPSENTID 83 >SPCC162.08c |nup211||nuclear pore complex associated protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 1837 Score = 26.6 bits (56), Expect = 9.4 Identities = 15/56 (26%), Positives = 28/56 (50%) Frame = +1 Query: 91 KLSTRVQGTAKQIEDLEKMAADNMQLTRDAKMKVGQANTDAREAEKQVSKGLEDLK 258 +L T++Q TAK+ + ++ + + K +V QANT ++ E+LK Sbjct: 1370 ELETKLQETAKETDTFKQQVNSLNEEVENLKKEVEQANTKNTRLAAAWNEKCENLK 1425 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 4,363,491 Number of Sequences: 5004 Number of extensions: 81978 Number of successful extensions: 233 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 219 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 233 length of database: 2,362,478 effective HSP length: 76 effective length of database: 1,982,174 effective search space used: 891978300 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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