BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= 030723E4_G07_e343_13.seq (1550 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC17G9.07 |rps2402|rps24-2|40S ribosomal protein S24|Schizosac... 155 1e-38 SPAC17G6.06 |rps2401|rps24-1, rps24|40S ribosomal protein S24|Sc... 150 6e-37 SPCC417.06c |ppk35|mug27|serine/threonine protein kinase Ppk35|S... 28 4.0 SPAC5D6.12 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||... 27 5.3 SPAC22A12.11 |dak1||dihydroxyacetone kinase Dak1|Schizosaccharom... 27 5.3 SPAC10F6.17c ||SPAC56E4.01c|mitochondrial pyruvate dehydrogenase... 27 5.3 SPBC4.02c |||conserved fungal protein|Schizosaccharomyces pombe|... 27 5.3 SPBC15C4.06c ||SPBC21H7.01c|ubiquitin-protein ligase E3 |Schizos... 27 9.2 >SPBC17G9.07 |rps2402|rps24-2|40S ribosomal protein S24|Schizosaccharomyces pombe|chr 2|||Manual Length = 134 Score = 155 bits (377), Expect = 1e-38 Identities = 79/122 (64%), Positives = 93/122 (76%), Gaps = 1/122 (0%) Frame = -3 Query: 519 MSEGTATIRTRKFMTNRLLARKQMVCDVLHPGKPTVSKTEIREKLAKMYKVTPDVVFVFG 340 MSE TIRTRKFMTNRLL RKQMV D+LHPGK +SK EIREKLA+MYK + V FG Sbjct: 1 MSEAV-TIRTRKFMTNRLLQRKQMVVDILHPGKANLSKNEIREKLAQMYKTDSECVQAFG 59 Query: 339 FKTNFGGGKSTGFALIYDTLDLAKKFEPKHRLARHGLYEK-KRPTRKQRKERKNRMKKVR 163 +T+FGGG+STGFALIYD+ + KKFEP +RL R G E ++ R+QRK+RKNR KKV Sbjct: 60 LRTHFGGGRSTGFALIYDSTESMKKFEPHYRLVRVGQAEPIQKVARQQRKQRKNRGKKVF 119 Query: 162 GT 157 GT Sbjct: 120 GT 121 >SPAC17G6.06 |rps2401|rps24-1, rps24|40S ribosomal protein S24|Schizosaccharomyces pombe|chr 1|||Manual Length = 134 Score = 150 bits (363), Expect = 6e-37 Identities = 74/122 (60%), Positives = 91/122 (74%), Gaps = 1/122 (0%) Frame = -3 Query: 519 MSEGTATIRTRKFMTNRLLARKQMVCDVLHPGKPTVSKTEIREKLAKMYKVTPDVVFVFG 340 MSE TIRTRKFMTNRLL RKQMV D+LHPGK +SK ++REKL +MYK V FG Sbjct: 1 MSEAV-TIRTRKFMTNRLLQRKQMVVDILHPGKANISKNDLREKLGQMYKTDASAVQAFG 59 Query: 339 FKTNFGGGKSTGFALIYDTLDLAKKFEPKHRLARHGLYEK-KRPTRKQRKERKNRMKKVR 163 +T++GGG++TGFALIYD ++ KKFEP +RL R G E ++ R+QRK+RKNR KKV Sbjct: 60 LRTHYGGGRTTGFALIYDDVEAMKKFEPHYRLVRVGHAEPIQKVARQQRKQRKNRGKKVF 119 Query: 162 GT 157 GT Sbjct: 120 GT 121 >SPCC417.06c |ppk35|mug27|serine/threonine protein kinase Ppk35|Schizosaccharomyces pombe|chr 3|||Manual Length = 624 Score = 27.9 bits (59), Expect = 4.0 Identities = 12/33 (36%), Positives = 18/33 (54%) Frame = -3 Query: 267 KFEPKHRLARHGLYEKKRPTRKQRKERKNRMKK 169 K+ P R G + +KR RK++ E+KN K Sbjct: 539 KYRPNARKPLVGRHREKRQLRKEKPEKKNNSTK 571 >SPAC5D6.12 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 314 Score = 27.5 bits (58), Expect = 5.3 Identities = 12/20 (60%), Positives = 15/20 (75%) Frame = +2 Query: 509 PSLIFDYFRDFGVKTHVSPS 568 P L+FDYF D G K HV+P+ Sbjct: 53 PDLLFDYFID-GHKIHVNPN 71 >SPAC22A12.11 |dak1||dihydroxyacetone kinase Dak1|Schizosaccharomyces pombe|chr 1|||Manual Length = 580 Score = 27.5 bits (58), Expect = 5.3 Identities = 11/28 (39%), Positives = 17/28 (60%) Frame = -3 Query: 402 EIREKLAKMYKVTPDVVFVFGFKTNFGG 319 +++E LAK YK+ P +F F T+ G Sbjct: 286 KVKEALAKEYKINPVRIFAGPFTTSLNG 313 >SPAC10F6.17c ||SPAC56E4.01c|mitochondrial pyruvate dehydrogenase |Schizosaccharomyces pombe|chr 1|||Manual Length = 444 Score = 27.5 bits (58), Expect = 5.3 Identities = 15/42 (35%), Positives = 20/42 (47%), Gaps = 1/42 (2%) Frame = +1 Query: 331 RLETEHENY-VWRHFVHFSELFTDLSFADGRFSWMQDIAYHL 453 RLE EH V R+ L +F D R+ W Q+I+ L Sbjct: 261 RLEVEHPGEEVLRNNRILGRLMPSRAFGDARYKWSQEISERL 302 >SPBC4.02c |||conserved fungal protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 456 Score = 27.5 bits (58), Expect = 5.3 Identities = 12/37 (32%), Positives = 17/37 (45%) Frame = -3 Query: 831 TKILPLTIFHSPFRXRNCWXGRSVRAXSLLRQLAKGG 721 T L T+F +P N W +++R LA GG Sbjct: 49 TNSLLQTLFQTPLPNANIWSNQAIRILMAFNILANGG 85 >SPBC15C4.06c ||SPBC21H7.01c|ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 556 Score = 26.6 bits (56), Expect = 9.2 Identities = 11/26 (42%), Positives = 18/26 (69%) Frame = -1 Query: 227 MKRRGRHANSVRNVRTE*RRYEVPRN 150 +KRR R N++R + E RRY +P++ Sbjct: 28 VKRRKRLFNAIRKLWNERRRYRMPKD 53 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 4,596,963 Number of Sequences: 5004 Number of extensions: 84531 Number of successful extensions: 223 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 203 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 221 length of database: 2,362,478 effective HSP length: 76 effective length of database: 1,982,174 effective search space used: 872156560 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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