SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 030723E4_G07_e343_13.seq
         (1550 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g04920.1 68416.m00534 40S ribosomal protein S24 (RPS24A) simi...   170   2e-42
At5g28060.1 68418.m03382 40S ribosomal protein S24 (RPS24B) 40S ...   166   4e-41
At1g18370.1 68414.m02295 kinesin motor family protein (NACK1) si...    31   2.0  
At3g45910.1 68416.m04968 hypothetical protein                          30   3.6  
At5g07570.1 68418.m00867 glycine/proline-rich protein contains s...    29   6.2  
At2g41790.1 68415.m05165 peptidase M16 family protein / insulina...    29   8.2  

>At3g04920.1 68416.m00534 40S ribosomal protein S24 (RPS24A) similar
           to ribosomal protein S19 GB:445612 [Solanum tuberosum]
           and similar to ribosomal protein S24 GB:4506703 [Homo
           sapiens]
          Length = 133

 Score =  170 bits (414), Expect = 2e-42
 Identities = 81/121 (66%), Positives = 99/121 (81%), Gaps = 1/121 (0%)
 Frame = -3

Query: 519 MSEGTATIRTRKFMTNRLLARKQMVCDVLHPGKPTVSKTEIREKLAKMYKVT-PDVVFVF 343
           M+E   TIRTRKFMTNRLL+RKQ V DVLHPG+  VSK E++EKLA+MY+V  P+ +FVF
Sbjct: 1   MAEKAVTIRTRKFMTNRLLSRKQFVIDVLHPGRANVSKAELKEKLARMYEVKDPNAIFVF 60

Query: 342 GFKTNFGGGKSTGFALIYDTLDLAKKFEPKHRLARHGLYEKKRPTRKQRKERKNRMKKVR 163
            F+T+FGGGKS+GF LIYDT++ AKKFEPK+RL R+GL  K   +RKQ KERKNR KK+R
Sbjct: 61  KFRTHFGGGKSSGFGLIYDTVESAKKFEPKYRLIRNGLDTKIEKSRKQIKERKNRAKKIR 120

Query: 162 G 160
           G
Sbjct: 121 G 121


>At5g28060.1 68418.m03382 40S ribosomal protein S24 (RPS24B) 40S
           ribosomal protein S19, Cyanophora paradoxa,
           EMBL:CPA245654
          Length = 133

 Score =  166 bits (403), Expect = 4e-41
 Identities = 79/121 (65%), Positives = 97/121 (80%), Gaps = 1/121 (0%)
 Frame = -3

Query: 519 MSEGTATIRTRKFMTNRLLARKQMVCDVLHPGKPTVSKTEIREKLAKMYKVT-PDVVFVF 343
           M+E   TIRTR FMTNRLLARKQ V DVLHPG+  VSK E++EKLA+MY+V  P+ +F F
Sbjct: 1   MAEKAVTIRTRNFMTNRLLARKQFVIDVLHPGRANVSKAELKEKLARMYEVKDPNAIFCF 60

Query: 342 GFKTNFGGGKSTGFALIYDTLDLAKKFEPKHRLARHGLYEKKRPTRKQRKERKNRMKKVR 163
            F+T+FGGGKS+G+ LIYDT++ AKKFEPK+RL R+GL  K   +RKQ KERKNR KK+R
Sbjct: 61  KFRTHFGGGKSSGYGLIYDTVENAKKFEPKYRLIRNGLDTKIEKSRKQIKERKNRAKKIR 120

Query: 162 G 160
           G
Sbjct: 121 G 121


>At1g18370.1 68414.m02295 kinesin motor family protein (NACK1)
           similar to kinesin heavy chain isolog GB:AAB63609
           GI:2262101 from [Arabidopsis thaliana]
          Length = 974

 Score = 31.1 bits (67), Expect = 2.0
 Identities = 21/74 (28%), Positives = 34/74 (45%)
 Frame = -3

Query: 570 KLGDTCVFTPKSRK*SKMSEGTATIRTRKFMTNRLLARKQMVCDVLHPGKPTVSKTEIRE 391
           K+  T   TP     S+  +   T+R R      LLA+ Q+  + ++     VSK +++E
Sbjct: 12  KMDRTPAVTPGGSSRSREEKIVVTVRLRPMNKRELLAKDQVAWECVN-DHTIVSKPQVQE 70

Query: 390 KLAKMYKVTPDVVF 349
           +L      T D VF
Sbjct: 71  RLHHQSSFTFDKVF 84


>At3g45910.1 68416.m04968 hypothetical protein 
          Length = 200

 Score = 30.3 bits (65), Expect = 3.6
 Identities = 17/49 (34%), Positives = 27/49 (55%), Gaps = 2/49 (4%)
 Frame = +2

Query: 470 LLVINLRVRIVAVPSLIFDYFRDFGVKTHVS--PSFTGKKKKKNSRGGP 610
           +L  +L V+  A+   + D  R FGV+ H+S  P+ T K+   N +G P
Sbjct: 75  ILDFDLFVQSNALNGGVIDMLRRFGVEVHISQPPTKTKKRSNANRKGFP 123


>At5g07570.1 68418.m00867 glycine/proline-rich protein contains
           similarity to flagelliform silk protein [Nephila
           clavipes] gi|7106224|gb|AAF36090
          Length = 1504

 Score = 29.5 bits (63), Expect = 6.2
 Identities = 13/26 (50%), Positives = 16/26 (61%)
 Frame = -3

Query: 360 DVVFVFGFKTNFGGGKSTGFALIYDT 283
           DV FV GF T+F  G   GFA+ + T
Sbjct: 251 DVGFVVGFVTSFAAGLDVGFAIDFAT 276


>At2g41790.1 68415.m05165 peptidase M16 family protein / insulinase
           family protein contains Pfam domain, PF05193: Peptidase
           M16 inactive domain; similar to insulin-degrading enzyme
           (Insulysin, Insulinase, Insulin protease) [Mouse]
           SWISS-PROT:Q9JHR7
          Length = 970

 Score = 29.1 bits (62), Expect = 8.2
 Identities = 16/55 (29%), Positives = 26/55 (47%), Gaps = 2/55 (3%)
 Frame = +1

Query: 301 KSGRFATTEVRLETEHENYVWRHFVHFSELFTDLSFADGRFSWMQ--DIAYHLLA 459
           K  RFA  +  +  E++NY +R   H +  +  L   D  + W +  D+  HL A
Sbjct: 615 KPDRFAVIKETVTKEYQNYKFRQPYHQAMYYCSLILQDQTWPWTEELDVLSHLEA 669


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 25,023,507
Number of Sequences: 28952
Number of extensions: 472702
Number of successful extensions: 1116
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 1060
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1109
length of database: 12,070,560
effective HSP length: 84
effective length of database: 9,638,592
effective search space used: 4163871744
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -