Samia BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 030723E4_G06_e335_14.seq
         (1525 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC1250.05 |rpl3002|rpl30-2, rpl30|60S ribosomal protein L30|Sc...   163   4e-41
SPAC9G1.03c |rpl3001|rpl30-1, rpl30|60S ribosomal protein L30|Sc...   160   5e-40
SPAC1834.01 |sup45||translation release factor eRF1|Schizosaccha...    29   1.3  
SPAC2F3.06c |kap104||karyopherin Kap104|Schizosaccharomyces pomb...    27   6.8  
SPAC977.14c |||aldo/keto reductase, unknown biological role|Schi...    27   9.0  
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr...    27   9.0  
SPCC1223.01 ||SPCC285.18|ubiquitin-protein ligase E3 |Schizosacc...    27   9.0  

>SPAC1250.05 |rpl3002|rpl30-2, rpl30|60S ribosomal protein
           L30|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 117

 Score =  163 bits (397), Expect = 4e-41
 Identities = 75/109 (68%), Positives = 91/109 (83%)
 Frame = +3

Query: 108 TAIIMVAAKKQKKTIESINSRLALVMKSGKYCLGYKQTLKTLRQGKAKLVIIARNAPPLR 287
           TA +   +KK KK+ ++INS+LAL MKSGKY LGYK TLKTLR GKAKL++IA NAPPLR
Sbjct: 7   TAPVAAVSKKGKKSGDTINSKLALTMKSGKYVLGYKSTLKTLRSGKAKLILIAANAPPLR 66

Query: 288 KSEIEYYALLAKTGVHHYSGNNIELGTACGKYYRVCTLAITDPGDSDII 434
           KSE+EYYA+L++  VHHYSGNNI+LGTACGK +RV  LA+ D GDSDI+
Sbjct: 67  KSELEYYAMLSRCSVHHYSGNNIDLGTACGKLFRVGVLAVIDAGDSDIL 115


>SPAC9G1.03c |rpl3001|rpl30-1, rpl30|60S ribosomal protein
           L30|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 109

 Score =  160 bits (388), Expect = 5e-40
 Identities = 73/104 (70%), Positives = 87/104 (83%)
 Frame = +3

Query: 123 VAAKKQKKTIESINSRLALVMKSGKYCLGYKQTLKTLRQGKAKLVIIARNAPPLRKSEIE 302
           V  KK KK+ ++IN++LAL MKSGKY LGYK TLKTLR GKAKL++IA N PPLRKSE+E
Sbjct: 4   VVTKKSKKSGDTINAKLALTMKSGKYVLGYKSTLKTLRSGKAKLILIAGNCPPLRKSELE 63

Query: 303 YYALLAKTGVHHYSGNNIELGTACGKYYRVCTLAITDPGDSDII 434
           YYA+L+K  VHHY+G NI+LGTACGK +RV  LAITD GDSDI+
Sbjct: 64  YYAMLSKANVHHYAGTNIDLGTACGKLFRVGVLAITDAGDSDIL 107


>SPAC1834.01 |sup45||translation release factor
           eRF1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 433

 Score = 29.5 bits (63), Expect = 1.3
 Identities = 13/42 (30%), Positives = 24/42 (57%)
 Frame = +3

Query: 135 KQKKTIESINSRLALVMKSGKYCLGYKQTLKTLRQGKAKLVI 260
           ++KK I+     ++L   SGKYC G   T+  L++G  + ++
Sbjct: 279 QEKKLIQRFFDEISL--DSGKYCFGVVDTMNALQEGAVETLL 318


>SPAC2F3.06c |kap104||karyopherin Kap104|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 910

 Score = 27.1 bits (57), Expect = 6.8
 Identities = 14/22 (63%), Positives = 15/22 (68%)
 Frame = -3

Query: 272 IPGDDHQLSFPLSECLQSLFVA 207
           IP DD  L FPL ECL S+ VA
Sbjct: 597 IPDDDPNL-FPLFECLSSVAVA 617


>SPAC977.14c |||aldo/keto reductase, unknown biological
           role|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 351

 Score = 26.6 bits (56), Expect = 9.0
 Identities = 14/45 (31%), Positives = 26/45 (57%)
 Frame = +3

Query: 243 KAKLVIIARNAPPLRKSEIEYYALLAKTGVHHYSGNNIELGTACG 377
           ++ +VI+++   P+RK  I+ +  L+  GVH    ++ EL   CG
Sbjct: 87  RSSIVILSKCFFPVRKDLIKIFGDLSSRGVHFL--DSPELANQCG 129


>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1275

 Score = 26.6 bits (56), Expect = 9.0
 Identities = 19/63 (30%), Positives = 31/63 (49%), Gaps = 5/63 (7%)
 Frame = +2

Query: 521 PIRPIVSRITIHWPSFYNVVTGKTLALPNLIALQ-----HIPLSPAGVIAKRPAPIALPN 685
           P  P+ S ++ H   +    +    +L N I+L      ++PLSP    A+ P+PI L +
Sbjct: 172 PRPPLPSSVSSHSSPYSTTSSTSLYSLYNDISLSCSPEPYLPLSPTRSPARTPSPIRLYS 231

Query: 686 SCA 694
           S A
Sbjct: 232 SDA 234


>SPCC1223.01 ||SPCC285.18|ubiquitin-protein ligase E3
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 732

 Score = 26.6 bits (56), Expect = 9.0
 Identities = 12/25 (48%), Positives = 15/25 (60%)
 Frame = +1

Query: 628 PPFASWRNSEEARTDRPSQQLRSLN 702
           PP AS RN  E  +  PS+Q  S+N
Sbjct: 353 PPGASGRNRRERTSSTPSEQSTSVN 377


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 4,671,677
Number of Sequences: 5004
Number of extensions: 87676
Number of successful extensions: 165
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 159
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 165
length of database: 2,362,478
effective HSP length: 76
effective length of database: 1,982,174
effective search space used: 854316994
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)