Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030723E4_G04_e319_14.seq
(1562 letters)
Database: nematostella
59,808 sequences; 16,821,457 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SB_55337| Best HMM Match : No HMM Matches (HMM E-Value=.) 208 7e-54
SB_46720| Best HMM Match : No HMM Matches (HMM E-Value=.) 201 1e-51
SB_39426| Best HMM Match : C4 (HMM E-Value=0) 167 2e-41
SB_5177| Best HMM Match : C4 (HMM E-Value=1.4e-32) 99 5e-21
SB_7057| Best HMM Match : No HMM Matches (HMM E-Value=.) 30 5.8
SB_41466| Best HMM Match : Collagen (HMM E-Value=9.2) 29 7.7
>SB_55337| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1853
Score = 208 bits (509), Expect = 7e-54
Identities = 87/145 (60%), Positives = 110/145 (75%)
Frame = +1
Query: 193 GFYFTIHSQTRLIPQCPAGTSSLWEGFSLIHIVANGKAHGQDLGAPGSCLRKFSTMPYMF 372
GF+ T HSQT +P+CP LWEG+SL+++ N +AHGQDLG+PGSC+++FSTMP++F
Sbjct: 1574 GFFLTRHSQTVRVPRCPEDRPPLWEGYSLLYVQGNERAHGQDLGSPGSCIQRFSTMPFLF 1633
Query: 373 CNLNNVCDFAQREDYSFWLSTPEPMPMAMTPIQARDVGTYISRCQVCEAPTRTIAVHSQS 552
CN+NNVC+ A R DYSFWLSTPEP+P M P+Q +V YI RC VCE + +AVHSQS
Sbjct: 1634 CNINNVCNLASRNDYSFWLSTPEPIP--MMPVQETNVQPYIGRCAVCEVSSHVMAVHSQS 1691
Query: 553 NTVPTCPNGWEELWIGYSFLMHTAG 627
TVP+CP+GW LW GYSFLM G
Sbjct: 1692 ITVPSCPSGWSPLWQGYSFLMPYIG 1716
Score = 163 bits (395), Expect = 5e-40
Identities = 72/124 (58%), Positives = 88/124 (70%), Gaps = 2/124 (1%)
Frame = +1
Query: 490 YISRCQVCEAPTRTIAVHSQSNTVPTCPNGWEELWIGYSFLMHT-AGADASGQSLISPGS 666
YI RC VCE + +AVHSQS TVP+CP+GW LW GYSFLMHT AG D SGQ L SPGS
Sbjct: 1714 YIGRCAVCEVSSHVMAVHSQSITVPSCPSGWSPLWQGYSFLMHTSAGNDGSGQLLSSPGS 1773
Query: 667 CLREFRNRPFIECNGLGRCNYFATAVSYWXSTIDDNKMFLKPEQKTLKADRV-TKVSRCA 843
CL +FR PFIEC+G G C+Y+ + S+W +T+D NK F P +TLKA + +VSRC
Sbjct: 1774 CLEDFRAHPFIECHGRGTCHYYGSTYSFWLATVDQNKQFRIPRPETLKAGNLRERVSRCQ 1833
Query: 844 VCMR 855
VC +
Sbjct: 1834 VCQK 1837
Score = 85.0 bits (201), Expect = 1e-16
Identities = 42/113 (37%), Positives = 63/113 (55%), Gaps = 5/113 (4%)
Frame = +1
Query: 208 IHSQTRLIPQCPAGTSSLWEGFS-LIHIVANGKAHGQDLGAPGSCLRKFSTMPYMFCNLN 384
+HSQ+ +P CP+G S LW+G+S L+H A GQ L +PGSCL F P++ C+
Sbjct: 1730 VHSQSITVPSCPSGWSPLWQGYSFLMHTSAGNDGSGQLLSSPGSCLEDFRAHPFIECHGR 1789
Query: 385 NVCDFAQREDYSFWLSTPEPMPMAMTP----IQARDVGTYISRCQVCEAPTRT 531
C + YSFWL+T + P ++A ++ +SRCQVC+ R+
Sbjct: 1790 GTCHY-YGSTYSFWLATVDQNKQFRIPRPETLKAGNLRERVSRCQVCQKNIRS 1841
Score = 70.9 bits (166), Expect = 3e-12
Identities = 42/104 (40%), Positives = 52/104 (50%), Gaps = 1/104 (0%)
Frame = +1
Query: 541 HSQSNTVPTCPNGWEELWIGYSFLMHTAGADASGQSLISPGSCLREFRNRPFIECNGLGR 720
HSQ+ VP CP LW GYS L A GQ L SPGSC++ F PF+ CN
Sbjct: 1580 HSQTVRVPRCPEDRPPLWEGYSLLYVQGNERAHGQDLGSPGSCIQRFSTMPFLFCNINNV 1639
Query: 721 CNYFA-TAVSYWXSTIDDNKMFLKPEQKTLKADRVTKVSRCAVC 849
CN + S+W ST + M P Q+T + + RCAVC
Sbjct: 1640 CNLASRNDYSFWLSTPEPIPMM--PVQET---NVQPYIGRCAVC 1678
>SB_46720| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1705
Score = 201 bits (491), Expect = 1e-51
Identities = 101/223 (45%), Positives = 137/223 (61%), Gaps = 2/223 (0%)
Frame = +1
Query: 193 GFYFTIHSQTRLIPQCPAGTSSLWEGFSLIHIVANGKAHGQDLGAPGSCLRKFSTMPYMF 372
GFY HSQT P+CP LW+G+SL+++ + +HGQDLG GSCL++F+TMPY++
Sbjct: 1460 GFYIVKHSQTTTPPECPPTYDKLWDGYSLLYVQGHDVSHGQDLGQAGSCLKRFTTMPYLY 1519
Query: 373 CNLNNVCDFAQREDYSFWLSTPEPMPMAMTPIQARDVGTYISRCQVCEAPTRTIAVHSQS 552
CN+ C++A R DYS+WLST +P M PIQA V YISRC VCE+ +AVHS+
Sbjct: 1520 CNIFGKCNYASRNDYSYWLSTDNQVP--MMPIQASAVEPYISRCTVCESHGPVMAVHSK- 1576
Query: 553 NTVPTCPNGWEELWIGYSFLMHT-AGADASGQSLISPGSCLREFRNRPFIECNGLGRCNY 729
+ HT AG +GQSL S GSCL FR PF EC+G G C+Y
Sbjct: 1577 ----------------HDLTNHTGAGGSGTGQSLGSSGSCLESFRPNPFTECHGRGTCHY 1620
Query: 730 FATAVSYWXSTIDDNKMFLKPEQKTLKA-DRVTKVSRCAVCMR 855
+A S+W +T+ N F + +TLKA + +++VSRC VC+R
Sbjct: 1621 YANKYSFWLATV--NTPFQTQQSETLKAGNLLSRVSRCQVCIR 1661
>SB_39426| Best HMM Match : C4 (HMM E-Value=0)
Length = 188
Score = 167 bits (406), Expect = 2e-41
Identities = 81/147 (55%), Positives = 100/147 (68%), Gaps = 2/147 (1%)
Frame = +1
Query: 421 FWLSTPEPMPMAMTPIQARDVGTYISRCQVCEAPTRTIAVHSQSNTVPTCPNGWEELWIG 600
+WLST +PM PIQA V YISRC VCE+ +AVHSQS T+PTCP GW LW G
Sbjct: 2 YWLSTDNQVPMM--PIQASAVEPYISRCTVCESHGPVMAVHSQSTTLPTCPGGWSSLWSG 59
Query: 601 YSFLMHT-AGADASGQSLISPGSCLREFRNRPFIECNGLGRCNYFATAVSYWXSTIDDNK 777
YSFLMHT AG +GQSL S GSCL FR PFIEC+G G C+Y+A S+W +T+ N
Sbjct: 60 YSFLMHTGAGGSGTGQSLGSSGSCLESFRPNPFIECHGRGTCHYYANKYSFWLATV--NT 117
Query: 778 MFLKPEQKTLKA-DRVTKVSRCAVCMR 855
F + +TLKA + +++VSRC VC+R
Sbjct: 118 PFQTQQSETLKAGNLLSRVSRCQVCIR 144
Score = 89.4 bits (212), Expect = 7e-18
Identities = 45/113 (39%), Positives = 65/113 (57%), Gaps = 3/113 (2%)
Frame = +1
Query: 184 KSRGFYFTIHSQTRLIPQCPAGTSSLWEGFS-LIHIVANGKAHGQDLGAPGSCLRKFSTM 360
+S G +HSQ+ +P CP G SSLW G+S L+H A G GQ LG+ GSCL F
Sbjct: 31 ESHGPVMAVHSQSTTLPTCPGGWSSLWSGYSFLMHTGAGGSGTGQSLGSSGSCLESFRPN 90
Query: 361 PYMFCNLNNVCDFAQREDYSFWLSTPE-PMPMAMT-PIQARDVGTYISRCQVC 513
P++ C+ C + + YSFWL+T P + ++A ++ + +SRCQVC
Sbjct: 91 PFIECHGRGTCHYYANK-YSFWLATVNTPFQTQQSETLKAGNLLSRVSRCQVC 142
>SB_5177| Best HMM Match : C4 (HMM E-Value=1.4e-32)
Length = 176
Score = 99 bits (238), Expect = 5e-21
Identities = 38/76 (50%), Positives = 54/76 (71%)
Frame = +1
Query: 193 GFYFTIHSQTRLIPQCPAGTSSLWEGFSLIHIVANGKAHGQDLGAPGSCLRKFSTMPYMF 372
GFY HSQT P+CP LW+G+SL+++ + +HGQDLG GSCL++F+TMPY++
Sbjct: 64 GFYIVKHSQTTTPPECPPTYDKLWDGYSLLYVQGHDVSHGQDLGQAGSCLKRFTTMPYLY 123
Query: 373 CNLNNVCDFAQREDYS 420
CN+ C++A R DYS
Sbjct: 124 CNIFGKCNYASRNDYS 139
Score = 70.1 bits (164), Expect = 4e-12
Identities = 32/67 (47%), Positives = 42/67 (62%), Gaps = 1/67 (1%)
Frame = +1
Query: 532 IAVHSQSNTVPTCPNGWEELWIGYSFLMHTAGADAS-GQSLISPGSCLREFRNRPFIECN 708
I HSQ+ T P CP +++LW GYS L++ G D S GQ L GSCL+ F P++ CN
Sbjct: 67 IVKHSQTTTPPECPPTYDKLWDGYS-LLYVQGHDVSHGQDLGQAGSCLKRFTTMPYLYCN 125
Query: 709 GLGRCNY 729
G+CNY
Sbjct: 126 IFGKCNY 132
>SB_7057| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 214
Score = 29.9 bits (64), Expect = 5.8
Identities = 13/34 (38%), Positives = 17/34 (50%)
Frame = +1
Query: 502 CQVCEAPTRTIAVHSQSNTVPTCPNGWEELWIGY 603
C VC P+R+ + S V CP GW + GY
Sbjct: 112 CAVCYVPSRSTQLMIPS--VARCPTGWSREYYGY 143
>SB_41466| Best HMM Match : Collagen (HMM E-Value=9.2)
Length = 288
Score = 29.5 bits (63), Expect = 7.7
Identities = 13/39 (33%), Positives = 18/39 (46%)
Frame = +1
Query: 502 CQVCEAPTRTIAVHSQSNTVPTCPNGWEELWIGYSFLMH 618
C VC P+R+ + + V CP GW + GY H
Sbjct: 151 CAVCYVPSRSTQLMIPA--VARCPAGWSREYYGYLMTSH 187
Database: nematostella
Posted date: Oct 22, 2007 1:22 PM
Number of letters in database: 16,821,457
Number of sequences in database: 59,808
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 35,773,383
Number of Sequences: 59808
Number of extensions: 715286
Number of successful extensions: 1696
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 1464
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1680
length of database: 16,821,457
effective HSP length: 85
effective length of database: 11,737,777
effective search space used: 5105932995
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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