BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= 030723E4_G04_e319_14.seq (1562 letters) Database: mosquito 2352 sequences; 563,979 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AF313909-1|AAL99382.1| 1024|Anopheles gambiae collagen IV alpha ... 269 2e-73 AF316638-1|AAG45166.1| 211|Anopheles gambiae glutathione S-tran... 25 5.9 >AF313909-1|AAL99382.1| 1024|Anopheles gambiae collagen IV alpha 1 chain protein. Length = 1024 Score = 269 bits (659), Expect = 2e-73 Identities = 120/224 (53%), Positives = 157/224 (70%), Gaps = 3/224 (1%) Frame = +1 Query: 193 GFYFTIHSQTRLIPQCPAGTSSLWEGFSLIHIVANGKAHGQDLGAPGSCLRKFSTMPYMF 372 G HSQ+ +P C G LW+G+SL+++ N H QDLG+ GSC+RKFST+P + Sbjct: 802 GILLVRHSQSDEVPVCEPGHLKLWDGYSLLYVDGNDYPHNQDLGSAGSCVRKFSTLPILA 861 Query: 373 CNLNNVCDFAQREDYSFWLSTPEPMPMAMTPIQARDVGTYISRCQVCEAPTRTIAVHSQS 552 C NNVC++A R D +FWLST P+P M P+ ++ YISRC VCEAPT IAVHSQ+ Sbjct: 862 CGQNNVCNYASRNDRTFWLSTSAPIP--MMPVTENEMRPYISRCTVCEAPTNVIAVHSQT 919 Query: 553 NTVPTCPNGWEELWIGYSFLMHTA-GADASGQSLISPGSCLREFRNRPFIECN-GLGRCN 726 +P CPNGW+ LWIGYSFLMHTA G GQSL PGSCL +FR PFIECN G G C+ Sbjct: 920 LHIPECPNGWDGLWIGYSFLMHTAVGHGGGGQSLSGPGSCLEDFRATPFIECNGGKGHCH 979 Query: 727 YFATAVSYWXSTIDDNKMFLKPEQKTLKA-DRVTKVSRCAVCMR 855 Y+ T S+W +++D++ F +PEQ+TLKA + +++VSRC VC+R Sbjct: 980 YYETQTSFWLVSLEDHQQFQRPEQQTLKAGNLLSRVSRCQVCIR 1023 Score = 25.0 bits (52), Expect = 5.9 Identities = 9/22 (40%), Positives = 14/22 (63%) Frame = +3 Query: 75 HEAYKXIQGRVGLKGFEGLQGI 140 ++ K QGR G+ G G+QG+ Sbjct: 213 NDGLKGFQGRKGMMGAPGIQGV 234 >AF316638-1|AAG45166.1| 211|Anopheles gambiae glutathione S-transferase D12 protein. Length = 211 Score = 25.0 bits (52), Expect = 5.9 Identities = 13/41 (31%), Positives = 19/41 (46%) Frame = +1 Query: 553 NTVPTCPNGWEELWIGYSFLMHTAGADASGQSLISPGSCLR 675 +T+PT + LW Y+ L++ A A SL C R Sbjct: 49 HTIPTLVDNGHILWESYAILIYLAEKYALDDSLYPKDVCER 89 Database: mosquito Posted date: Oct 23, 2007 1:18 PM Number of letters in database: 563,979 Number of sequences in database: 2352 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,192,125 Number of Sequences: 2352 Number of extensions: 22895 Number of successful extensions: 67 Number of sequences better than 10.0: 2 Number of HSP's better than 10.0 without gapping: 56 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 59 length of database: 563,979 effective HSP length: 67 effective length of database: 406,395 effective search space used: 184096935 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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