BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= 030723E4_G03_e311_13.seq (1629 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC5D6.12 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||... 29 2.4 SPAC4G9.19 |||DNAJ domain protein DNAJB family|Schizosaccharomyc... 28 3.2 SPBC28F2.12 |rpb1||DNA-directed RNA polymerase II large subunit|... 28 4.2 SPBC405.04c |ypt7||GTPase Ypt7|Schizosaccharomyces pombe|chr 2||... 27 7.4 SPAC31A2.09c |apm4||AP-2 adaptor complex subunit Apm4 |Schizosac... 27 9.8 >SPAC5D6.12 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 314 Score = 28.7 bits (61), Expect = 2.4 Identities = 17/54 (31%), Positives = 24/54 (44%) Frame = +3 Query: 498 QVIPVPQQHEEKTIVYVLSKKQDQPQDILVPQIENKPPTKPEVYFVKYKNKEDS 659 Q P+ Q E + +Y S D P+ + PP P+V+F K K DS Sbjct: 102 QKYPLSDQVENSSNIYDPSSPPDSPRKQQT-HLGTIPPEHPDVFFHNLKKKLDS 154 >SPAC4G9.19 |||DNAJ domain protein DNAJB family|Schizosaccharomyces pombe|chr 1|||Manual Length = 270 Score = 28.3 bits (60), Expect = 3.2 Identities = 12/36 (33%), Positives = 20/36 (55%) Frame = +3 Query: 375 FVHTAPEEPEVPTQRKPIVLPPAQKHYKIIFVKTPS 482 F+ + ++PTQ+KP LPP ++ + TPS Sbjct: 234 FLILREQSKQIPTQQKPSSLPPPERALPAPTMPTPS 269 >SPBC28F2.12 |rpb1||DNA-directed RNA polymerase II large subunit|Schizosaccharomyces pombe|chr 2|||Manual Length = 1752 Score = 27.9 bits (59), Expect = 4.2 Identities = 17/43 (39%), Positives = 22/43 (51%) Frame = +3 Query: 186 YPSSVNIGYGSSNLASPIYSSFSSGDSLSPNYNEYQNQYLTTA 314 + S+++ GYG L SP YS S G S SP Y Y T+ Sbjct: 1543 FSSAMSPGYG---LTSPSYSPSSPGYSTSPAYMPSSPSYSPTS 1582 >SPBC405.04c |ypt7||GTPase Ypt7|Schizosaccharomyces pombe|chr 2|||Manual Length = 205 Score = 27.1 bits (57), Expect = 7.4 Identities = 11/31 (35%), Positives = 19/31 (61%) Frame = +1 Query: 745 QTDSVLAVHRDLSDPVHRDLSDPVHRDLSAQ 837 +T + LA+ SD + D +DP+H D+ +Q Sbjct: 169 ETVAKLALENMDSDDIAADFTDPIHLDMESQ 199 >SPAC31A2.09c |apm4||AP-2 adaptor complex subunit Apm4 |Schizosaccharomyces pombe|chr 1|||Manual Length = 446 Score = 26.6 bits (56), Expect = 9.8 Identities = 13/68 (19%), Positives = 32/68 (47%), Gaps = 1/68 (1%) Frame = +3 Query: 381 HTAPEEPEVPTQRKPIVLPPAQKHYKIIFVKTPSESRTP-QVIPVPQQHEEKTIVYVLSK 557 H PE + + +PP +++ ++ P +++P+ +Q ++ I+Y +S Sbjct: 262 HQCVRLPEFENEHRITFIPP-DGEVELMSYRSHENINIPFRIVPIVEQLSKQKIIYRISI 320 Query: 558 KQDQPQDI 581 + D P + Sbjct: 321 RADYPHKL 328 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 4,246,301 Number of Sequences: 5004 Number of extensions: 76163 Number of successful extensions: 243 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 227 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 243 length of database: 2,362,478 effective HSP length: 76 effective length of database: 1,982,174 effective search space used: 923693084 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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