BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= 030723E4_G03_e311_13.seq (1629 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g67710.1 68414.m07727 two-component responsive regulator fami... 30 3.8 At1g28290.1 68414.m03472 pollen Ole e 1 allergen and extensin fa... 30 5.0 At2g27380.1 68415.m03302 proline-rich family protein contains pr... 29 6.6 At5g05840.1 68418.m00642 expressed protein contains Pfam profile... 29 8.7 At1g78700.1 68414.m09173 brassinosteroid signalling positive reg... 29 8.7 >At1g67710.1 68414.m07727 two-component responsive regulator family protein / response regulator family protein contains Pfam profile: PF00072 response regulator receiver domain; contains similarity to response regulator proteins from [Arabidopsis thaliana] Length = 521 Score = 30.3 bits (65), Expect = 3.8 Identities = 20/69 (28%), Positives = 31/69 (44%), Gaps = 3/69 (4%) Frame = +3 Query: 426 IVLPPAQKHYKIIFVKTPSESRTPQVIP---VPQQHEEKTIVYVLSKKQDQPQDILVPQI 596 +++PP K +I F S S ++P VP+ E K ++Y S Q QP + Sbjct: 321 VIMPPKTKKTRIGFDPPISSSAFDSLLPWNDVPEVLESKPVLYENSFLQQQPLPSQSSYV 380 Query: 597 ENKPPTKPE 623 N P+ E Sbjct: 381 ANSAPSLME 389 >At1g28290.1 68414.m03472 pollen Ole e 1 allergen and extensin family protein similar to arabinogalactan protein [Daucus carota] GI:11322245; contains Pfam profile PF01190: Pollen proteins Ole e I family Length = 359 Score = 29.9 bits (64), Expect = 5.0 Identities = 26/83 (31%), Positives = 35/83 (42%), Gaps = 3/83 (3%) Frame = +3 Query: 387 APEEPEVPTQRKPIVLPPAQKHYKI-IFVKTPSESRTPQVIPV--PQQHEEKTIVYVLSK 557 AP +P KP V PPA+ K ++ T + + P PV P K V +K Sbjct: 98 APVKPPTKPPVKPPVSPPAKPPVKPPVYPPTKAPVKPPTKPPVKPPVYPPTKAPVKPPTK 157 Query: 558 KQDQPQDILVPQIENKPPTKPEV 626 +P + KPPTKP V Sbjct: 158 PPVKPPVYPPTKAPVKPPTKPPV 180 >At2g27380.1 68415.m03302 proline-rich family protein contains proline-rich extensin domains, INTERPRO:IPR002965 Length = 761 Score = 29.5 bits (63), Expect = 6.6 Identities = 27/84 (32%), Positives = 34/84 (40%), Gaps = 8/84 (9%) Frame = +3 Query: 393 EEPEVPTQRKPIVLPPAQK--------HYKIIFVKTPSESRTPQVIPVPQQHEEKTIVYV 548 ++P PT PI PP QK K+ VK P+ +P V P P H+ T +Y Sbjct: 392 QKPPTPTYSPPIKPPPLQKPPTPTYSPPIKLPPVKPPTPIYSPPVKP-PPVHKPPTPIY- 449 Query: 549 LSKKQDQPQDILVPQIENKPPTKP 620 S P P PP KP Sbjct: 450 -SPPVKPPPVHKPPTPTYSPPIKP 472 >At5g05840.1 68418.m00642 expressed protein contains Pfam profile PF04788: Protein of unknown function (DUF620); expression supported by MPSS Length = 439 Score = 29.1 bits (62), Expect = 8.7 Identities = 11/38 (28%), Positives = 21/38 (55%), Gaps = 1/38 (2%) Frame = +1 Query: 769 HRDLSDPVHRDLSD-PVHRDLSAQVHRDYSXLVCRDRS 879 H D +P+H+D+ D P+ ++ + + Y V DR+ Sbjct: 91 HNDYENPIHKDIKDQPLEMSMAQYIVKQYIAAVGGDRA 128 >At1g78700.1 68414.m09173 brassinosteroid signalling positive regulator-related contains similarity to BZR1 protein [Arabidopsis thaliana] gi|20270971|gb|AAM18490 Length = 325 Score = 29.1 bits (62), Expect = 8.7 Identities = 32/114 (28%), Positives = 45/114 (39%), Gaps = 5/114 (4%) Frame = +3 Query: 183 YYPS---SVNIGYGSSNLASPIYSSFSSGDSLSPNYNEYQNQYLTTAAXXXXXXXXXXXX 353 Y PS S N GSSN SP SSF++ S ++L+T + Sbjct: 96 YQPSPCASYNPSPGSSNFMSPASSSFANLTSGDGQSLIPWLKHLSTTSSSSASSSSRLPN 155 Query: 354 XXXXXXXFVHTAPEEPEV--PTQRKPIVLPPAQKHYKIIFVKTPSESRTPQVIP 509 + +AP P + PT R P + Q+ FV + S T Q+IP Sbjct: 156 YLYIPGGSI-SAPVTPPLSSPTARTPRMNTDWQQLNNSFFVSSTPPSPTRQIIP 208 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 23,031,318 Number of Sequences: 28952 Number of extensions: 431758 Number of successful extensions: 1126 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 1048 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1115 length of database: 12,070,560 effective HSP length: 85 effective length of database: 9,609,640 effective search space used: 4391605480 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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