Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030723E4_G02_e303_14.seq
(1491 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g73050.1 68414.m08447 (R)-mandelonitrile lyase, putative / (R... 54 2e-07
At5g51930.1 68418.m06442 glucose-methanol-choline (GMC) oxidored... 54 3e-07
At1g14185.1 68414.m01678 glucose-methanol-choline (GMC) oxidored... 52 7e-07
At1g12570.1 68414.m01459 glucose-methanol-choline (GMC) oxidored... 50 4e-06
At3g56060.1 68416.m06229 glucose-methanol-choline (GMC) oxidored... 48 2e-05
At1g14190.1 68414.m01679 glucose-methanol-choline (GMC) oxidored... 47 3e-05
At1g72970.1 68414.m08439 glucose-methanol-choline (GMC) oxidored... 45 1e-04
At5g51950.1 68418.m06447 glucose-methanol-choline (GMC) oxidored... 44 2e-04
At1g78580.1 68414.m09158 alpha, alpha-trehalose-phosphate syntha... 33 0.64
At5g48130.1 68418.m05945 phototropic-responsive NPH3 family prot... 30 3.4
At1g67120.1 68414.m07636 midasin-related similar to Midasin (MID... 30 3.4
At3g16950.1 68416.m02166 dihydrolipoamide dehydrogenase 1, plast... 29 5.9
At2g46240.1 68415.m05750 IQ domain-containing protein / BAG doma... 29 5.9
At1g63360.1 68414.m07162 disease resistance protein (CC-NBS-LRR ... 29 7.9
>At1g73050.1 68414.m08447 (R)-mandelonitrile lyase, putative /
(R)-oxynitrilase, putative similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|52707];
contains Pfam protile PF00732 GMC oxidoreductase
Length = 552
Score = 54.0 bits (124), Expect = 2e-07
Identities = 43/128 (33%), Positives = 65/128 (50%), Gaps = 12/128 (9%)
Frame = +3
Query: 84 GKXIGVGRFSHTINNGQRHSTLTALLNKSKKPNLFVLKNAIVTKIL------TENNTVLG 245
G IG F T G+RHS+ LL ++ N+ V A V ++L + V
Sbjct: 207 GTKIGGSTFDRT---GRRHSSAD-LLRYARSSNIRVAVYATVERVLLASSPSVSGSNVSA 262
Query: 246 VKVIKQGKELKFF-----TNKEVVISAGTFNTAKLLFLSGIGPKDHLDSLGIDVVQDLP- 407
+ V+ + + +F EV++SAG + +LLFLSGIGP+ +L + GI V D P
Sbjct: 263 IGVVYRDQLGRFHHALIRDRGEVILSAGALGSPQLLFLSGIGPRSYLSTWGIPVALDQPH 322
Query: 408 VGDNLQDH 431
VGD + D+
Sbjct: 323 VGDFVYDN 330
>At5g51930.1 68418.m06442 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 582
Score = 53.6 bits (123), Expect = 3e-07
Identities = 67/267 (25%), Positives = 119/267 (44%), Gaps = 10/267 (3%)
Frame = +3
Query: 129 GQRHSTLTALLNKSKKPNLFVLKNAIVTKIL--TENNTVL--GVKVIKQGKELKFFTNKE 296
G RH T LL + + V +A V K+L TE VL + L E
Sbjct: 235 GHRH-TAANLLEYANPNRIVVYLHASVHKVLFTTEAYEVLFEDANGVFHKANLANKATNE 293
Query: 297 VVISAGTFNTAKLLFLSGIGPKDHLDSLGID-VVQDLP-VGDNLQDHVMILTYIAVDNGT 470
V++SAG + +LL LSG+GP HL++ G++ +V D P VG + D+ M ++A+ +
Sbjct: 294 VILSAGALGSPQLLMLSGVGPAVHLEAHGVNPLVLDQPMVGQGMADNPM--NFVAIPSPQ 351
Query: 471 CIPDESTEAMDVIRYLYNRSGSLSRHESMAAYLPLNKDVPNLPEYGFYPVCIPQYTSFYQ 650
+ +A+ + ++ S E ++ L L+ D+ G + + TS
Sbjct: 352 PVELSLIQAVGITKF-------DSYIEGLSG-LSLSFDITRRFFDGV--LNLLNETSHTT 401
Query: 651 SCMIIGFNSDICTKLHDIIQNYEVLSIATLLLK----PKSRGKVRXKSTDPEEKPLIYSG 818
S I+ + + K D+ EV L+ + P S+G ++ ++T+P + P +
Sbjct: 402 SRKILTQSIAVLLKSFDV--KLEVRMNGGLIFQKVDGPASKGHMKLRNTNPRDNPSVTFN 459
Query: 819 XLDNPEDXDPIPRLIKTAWXLANTAYF 899
PED + + + T + N+ F
Sbjct: 460 YYQEPEDLNKCVKGLNTIIRMINSKAF 486
>At1g14185.1 68414.m01678 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 503
Score = 52.4 bits (120), Expect = 7e-07
Identities = 43/131 (32%), Positives = 66/131 (50%), Gaps = 10/131 (7%)
Frame = +3
Query: 78 TRGKXIGVGRFSHTINNGQRHSTLTALLNKSKKPNLFVLKNAIVTKILTENNTV--LGVK 251
T+G IG F G+RH T LL K + VL NA V I+ + N +GV+
Sbjct: 186 TQGTKIGGSIFDQC---GKRH-TSADLLGYGKPNCITVLLNATVKSIIFDANKTRAVGVR 241
Query: 252 VIKQ----GKELKFFTNK---EVVISAGTFNTAKLLFLSGIGPKDHLDSLGIDVVQDL-P 407
++ K K + EV+++AG + ++L LSGIGP++HL+ I V+ +L
Sbjct: 242 FMESDGNSSKSYKVHVEQHRGEVILAAGALGSPQILLLSGIGPENHLNDFDIPVIVNLKE 301
Query: 408 VGDNLQDHVMI 440
VG + D+ I
Sbjct: 302 VGKQMSDNPAI 312
Score = 34.3 bits (75), Expect = 0.21
Identities = 13/46 (28%), Positives = 26/46 (56%)
Frame = +3
Query: 726 SIATLLLKPKSRGKVRXKSTDPEEKPLIYSGXLDNPEDXDPIPRLI 863
+I+ + PKS+G+++ +T+P E P + L+N D D ++
Sbjct: 356 AISAKIAFPKSKGRLKLNNTNPRENPSVTFNYLENKADLDACQEMV 401
>At1g12570.1 68414.m01459 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 572
Score = 50.0 bits (114), Expect = 4e-06
Identities = 43/129 (33%), Positives = 63/129 (48%), Gaps = 13/129 (10%)
Frame = +3
Query: 105 RFSHTI--NNGQRHSTLTALLNKSKKPNLFVLKNAIVTKIL--TENNTV-LGVKVIKQGK 269
+F TI NG RH T LL + + VL +A V +IL T T + V+ + +
Sbjct: 198 KFGGTIFDRNGNRH-TAADLLEYADPKGITVLLHATVHRILFRTRGTTKPIANGVVYRDR 256
Query: 270 E-------LKFFTNKEVVISAGTFNTAKLLFLSGIGPKDHLDSLGIDVVQDLP-VGDNLQ 425
LK E+++SAGT + +LL LSG+GP L + I VV D P VG +
Sbjct: 257 TGQAHRAYLKEGALSEIILSAGTLGSPQLLMLSGVGPSAQLQAQNITVVMDQPHVGQGMY 316
Query: 426 DHVMILTYI 452
D+ M ++
Sbjct: 317 DNPMNAVFV 325
>At3g56060.1 68416.m06229 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 577
Score = 47.6 bits (108), Expect = 2e-05
Identities = 46/187 (24%), Positives = 84/187 (44%), Gaps = 2/187 (1%)
Frame = +3
Query: 285 TNKEVVISAGTFNTAKLLFLSGIGPKDHLDSLGID-VVQDLP-VGDNLQDHVMILTYIAV 458
T EV++SAG + +LL LSG+GP HL + ++ V+ D P VG + D+ M + +
Sbjct: 271 TMSEVILSAGAIASPQLLMLSGVGPAAHLAAYRVNPVIVDQPMVGQGMGDNPM--NPVFI 328
Query: 459 DNGTCIPDESTEAMDVIRYLYNRSGSLSRHESMAAYLPLNKDVPNLPEYGFYPVCIPQYT 638
+ + +A+ + ++ G + S++ V NL + P +
Sbjct: 329 PSPEPVEVSLVQAVGITKFGSYIEGGSALSLSISLTRSFFDGVLNLLKKTKLPT--QSIS 386
Query: 639 SFYQSCMIIGFNSDICTKLHDIIQNYEVLSIATLLLKPKSRGKVRXKSTDPEEKPLIYSG 818
F++S + ++ TK IIQ P SRG + ++T+P++ P +
Sbjct: 387 KFFKS---LDLTLNVTTKAGVIIQKVN---------GPLSRGHLELRNTNPDDNPSVTFN 434
Query: 819 XLDNPED 839
+PED
Sbjct: 435 YFKDPED 441
>At1g14190.1 68414.m01679 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 501
Score = 47.2 bits (107), Expect = 3e-05
Identities = 42/132 (31%), Positives = 65/132 (49%), Gaps = 11/132 (8%)
Frame = +3
Query: 78 TRGKXIGVGRFSHTINNGQRHSTLTALLNKSKKPNLFVLKNAIVTKIL--TENNT-VLGV 248
T+G IG + G+RH T LL K + VL N V I+ + N T +GV
Sbjct: 165 TQGTKIGGSIYDQC---GKRH-TSADLLGFGKPNCITVLLNTTVKSIIFDSSNKTRAVGV 220
Query: 249 KVIKQ----GKELKFFTNK---EVVISAGTFNTAKLLFLSGIGPKDHLDSLGIDVVQDL- 404
+ ++ K K + EV+++AG + ++L LSGIGP++HL I V+ +L
Sbjct: 221 RFMESDGNSSKSYKVHVEQHRGEVILAAGALGSPQILLLSGIGPENHLKDFDIPVIVNLK 280
Query: 405 PVGDNLQDHVMI 440
VG + D+ I
Sbjct: 281 EVGRKMSDNPAI 292
Score = 37.9 bits (84), Expect = 0.017
Identities = 16/41 (39%), Positives = 25/41 (60%)
Frame = +3
Query: 723 LSIATLLLKPKSRGKVRXKSTDPEEKPLIYSGXLDNPEDXD 845
+SIA + PKS+G+++ ST+P E P + L+N D D
Sbjct: 335 ISIAAKIAFPKSKGRLKLNSTNPRENPSVKFNYLENKADLD 375
>At1g72970.1 68414.m08439 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 594
Score = 45.2 bits (102), Expect = 1e-04
Identities = 40/113 (35%), Positives = 58/113 (51%), Gaps = 10/113 (8%)
Frame = +3
Query: 129 GQRHSTLTALLNKSKKPNLFVLKNAIVTKILTENN----TVLGV--KVIKQGKELKFFTN 290
G+RH T LL + L VL A V KI+ + + V GV K K + +N
Sbjct: 225 GRRH-TAAELLAYANPQKLRVLIYATVQKIVFDTSGTRPRVTGVIFKDEKGNQHQALLSN 283
Query: 291 K---EVVISAGTFNTAKLLFLSGIGPKDHLDSLGIDVV-QDLPVGDNLQDHVM 437
+ EV++S+G + ++L LSGIGPK L L I VV ++ VG + D+ M
Sbjct: 284 RKGSEVILSSGAIGSPQMLMLSGIGPKKELQRLKIPVVLENEHVGKGMADNPM 336
>At5g51950.1 68418.m06447 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 586
Score = 44.4 bits (100), Expect = 2e-04
Identities = 42/120 (35%), Positives = 57/120 (47%), Gaps = 12/120 (10%)
Frame = +3
Query: 129 GQRHSTLTALLNKSKKPNLFVLKNAIVTKILTENN-----TVLGV-----KVIKQGKELK 278
G RH T LL + N+ V +A V KIL GV + EL+
Sbjct: 208 GHRH-TAADLLEYANPGNIVVYLHASVHKILFTTKGRPRPKAYGVIFQDANGVLHKAELE 266
Query: 279 FFTNKEVVISAGTFNTAKLLFLSGIGPKDHLDSLGI-DVVQDLP-VGDNLQDHVMILTYI 452
+ EV++SAG + +LL LSGIGP HL + GI +V D P VG + D+ M +I
Sbjct: 267 KNSMNEVILSAGAIGSPQLLMLSGIGPAAHLAAHGIKPLVLDHPMVGQGMGDNPMNAIFI 326
>At1g78580.1 68414.m09158 alpha, alpha-trehalose-phosphate synthase,
UDP-forming, putative / trehalose-6-phosphate synthase,
putative / UDP-glucose-glucosephosphate
glucosyltransferase, putative similar to
trehalose-6-phosphate synthase SL-TPS/P [Selaginella
lepidophylla] GI:4100325; contains Pfam profiles
PF00982: Glycosyltransferase family 20, PF02358:
Trehalose-phosphatase
Length = 942
Score = 32.7 bits (71), Expect = 0.64
Identities = 20/86 (23%), Positives = 42/86 (48%)
Frame = +3
Query: 561 AYLPLNKDVPNLPEYGFYPVCIPQYTSFYQSCMIIGFNSDICTKLHDIIQNYEVLSIATL 740
A L ++K P LP++ I +Y+ +I+GFN+ + + + + + + L
Sbjct: 564 AQLRISKVPPELPQHD----AIQRYSKSNNRLLILGFNATLTEPVDNQGRRGDQIKEMDL 619
Query: 741 LLKPKSRGKVRXKSTDPEEKPLIYSG 818
L P+ +G ++ +DP ++ SG
Sbjct: 620 NLHPELKGPLKALCSDPSTTIVVLSG 645
>At5g48130.1 68418.m05945 phototropic-responsive NPH3 family protein
contains NPH3 family domain, Pfam:PF03000
Length = 625
Score = 30.3 bits (65), Expect = 3.4
Identities = 21/75 (28%), Positives = 36/75 (48%), Gaps = 2/75 (2%)
Frame = +3
Query: 231 NTVLGVKVIKQGKELKFFTNKEVVISAGTFNTAKLLFLSGIGP-KDHLDSLG-IDVVQDL 404
+T+L +KV+ KE + V + +FN K L + G K+ D L I++ Q+
Sbjct: 23 STLLKIKVLSWSKETGLPASVHVRVCNKSFNLHKSLLCAKSGYFKEREDQLSEIEIPQEF 82
Query: 405 PVGDNLQDHVMILTY 449
P G + +M+ Y
Sbjct: 83 PGGAETFEKIMLFIY 97
>At1g67120.1 68414.m07636 midasin-related similar to Midasin
(MIDAS-containing protein) (Swiss-Prot:Q12019)
[Saccharomyces cerevisiae]; similar to Midasin
(MIDAS-containing protein) (Swiss-Prot:Q9NU22) [Homo
sapiens]; contains Prosite PS00017: ATP/GTP-binding site
motif A (P-loop)
Length = 5336
Score = 30.3 bits (65), Expect = 3.4
Identities = 22/82 (26%), Positives = 36/82 (43%), Gaps = 1/82 (1%)
Frame = +3
Query: 684 CTKLHDIIQNYEVLSIATLLLKPKSRGKVRXKSTDPEEKPLIYSGXLDNPEDXDPIPRL- 860
C + + I + EV S +L + R +TD +E IY D +P PR+
Sbjct: 1953 CQFMQEAIHDLEVESFLNVLYIQRMR-----TATDRKEVLRIYKAIFDKTPSINPYPRVQ 2007
Query: 861 IKTAWXLANTAYFKGRMRKSXI 926
+ A+ + TA K + +S I
Sbjct: 2008 LNPAYLVVGTAAIKRNLNQSNI 2029
>At3g16950.1 68416.m02166 dihydrolipoamide dehydrogenase 1,
plastidic / lipoamide dehydrogenase 1 (PTLPD1) identical
to plastidic lipoamide dehydrogenase from Arabidopsis
thaliana [gi:7159282]
Length = 570
Score = 29.5 bits (63), Expect = 5.9
Identities = 17/46 (36%), Positives = 26/46 (56%)
Frame = +3
Query: 180 NLFVLKNAIVTKILTENNTVLGVKVIKQGKELKFFTNKEVVISAGT 317
NL AI ILT +VLG + +K GK+ T K+++I+ G+
Sbjct: 182 NLTNSMKAIGVDILTGFGSVLGPQKVKYGKD-NIITAKDIIIATGS 226
>At2g46240.1 68415.m05750 IQ domain-containing protein / BAG
domain-containing protein contains Pfam profiles
PF00612: IQ calmodulin-binding motif, PF02179: BAG
(Apoptosis regulator Bcl-2 protein) domain
Length = 1043
Score = 29.5 bits (63), Expect = 5.9
Identities = 17/51 (33%), Positives = 24/51 (47%), Gaps = 2/51 (3%)
Frame = +3
Query: 513 YLYNRSGSLSRHESMAAYLPLNKDVP--NLPEYGFYPVCIPQYTSFYQSCM 659
Y Y G+ S+H +M A P + N P Y +P C P ++Q CM
Sbjct: 19 YYYQGFGNNSQHMAMDAPPPCHGSCVHGNFPAY--WPPCYPPQVPYHQCCM 67
>At1g63360.1 68414.m07162 disease resistance protein (CC-NBS-LRR
class), putative domain signature CC-NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 884
Score = 29.1 bits (62), Expect = 7.9
Identities = 10/30 (33%), Positives = 19/30 (63%)
Frame = +3
Query: 690 KLHDIIQNYEVLSIATLLLKPKSRGKVRXK 779
K+H ++ +YE + + TLLL + G +R +
Sbjct: 526 KIHHLVGSYECMELTTLLLGKREYGSIRSQ 555
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,386,041
Number of Sequences: 28952
Number of extensions: 351391
Number of successful extensions: 933
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 897
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 933
length of database: 12,070,560
effective HSP length: 84
effective length of database: 9,638,592
effective search space used: 3971099904
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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