BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= 030723E4_F06_e334_12.seq (1590 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC306.02c |||Rab GTPase binding |Schizosaccharomyces pombe|chr... 46 1e-05 SPBC543.07 |pek1|skh1, mkk1|MAP kinase kinase Pek1 |Schizosaccha... 29 1.8 SPBC26H8.03 |cho2||phosphatidylethanolamine N-methyltransferase ... 27 7.2 SPBP35G2.10 |mit1||SHREC complex subunit Mit1|Schizosaccharomyce... 27 7.2 SPCC285.11 |ucp10||UBA/UAS domain protein Ucp10|Schizosaccharomy... 27 9.5 SPAC11E3.05 |||ubiquitin-protein ligase E3|Schizosaccharomyces p... 27 9.5 >SPCC306.02c |||Rab GTPase binding |Schizosaccharomyces pombe|chr 3|||Manual Length = 171 Score = 46.0 bits (104), Expect = 1e-05 Identities = 29/99 (29%), Positives = 47/99 (47%), Gaps = 3/99 (3%) Frame = +3 Query: 99 FQANYLMVFLGLFAYCLITSPLLLIAM---VASFFGYRKLTSGPNTWKIGSWELTKTQQY 269 F +NYL + L Y LI +PLLLI + V +G RKL ++ ++ Y Sbjct: 61 FSSNYLAIIAMLVIYALIRNPLLLIVIGIGVGGVYGIRKLQGA--DLQLSHRVISNQNLY 118 Query: 270 AVAAAGSMGICWLAGAGAVLFWVLGATVTVVALHASFFD 386 + A + + A + W++GA+ V HA+FF+ Sbjct: 119 VILACVLIPLGLFASPIETIIWLVGASCVCVFGHAAFFE 157 >SPBC543.07 |pek1|skh1, mkk1|MAP kinase kinase Pek1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 363 Score = 29.1 bits (62), Expect = 1.8 Identities = 17/50 (34%), Positives = 23/50 (46%) Frame = +3 Query: 333 WVLGATVTVVALHASFFDAESLPPSDDPEQFPMMEQV*HSPLPLFMPPPG 482 W LG T+ VAL+ F E PP P ++ + + P PL PG Sbjct: 263 WSLGLTLMEVALNRFPFPPEGSPP---PMPIELLSYIINMPPPLLPQEPG 309 >SPBC26H8.03 |cho2||phosphatidylethanolamine N-methyltransferase Cho2|Schizosaccharomyces pombe|chr 2|||Manual Length = 905 Score = 27.1 bits (57), Expect = 7.2 Identities = 14/32 (43%), Positives = 18/32 (56%) Frame = +3 Query: 237 GSWELTKTQQYAVAAAGSMGICWLAGAGAVLF 332 G +EL Y+V AG G+ L G+ AVLF Sbjct: 287 GVFELAPHPMYSVGYAGYYGMSLLTGSYAVLF 318 >SPBP35G2.10 |mit1||SHREC complex subunit Mit1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1418 Score = 27.1 bits (57), Expect = 7.2 Identities = 10/29 (34%), Positives = 17/29 (58%), Gaps = 1/29 (3%) Frame = +1 Query: 445 DTVPCRC-SCRHLGVYCRSTVSTLKLCII 528 D V CRC +HL +C ++ L++C + Sbjct: 1307 DNVRCRCCGIKHLPAHCPLSIVPLEICFL 1335 >SPCC285.11 |ucp10||UBA/UAS domain protein Ucp10|Schizosaccharomyces pombe|chr 3|||Manual Length = 427 Score = 26.6 bits (56), Expect = 9.5 Identities = 16/51 (31%), Positives = 24/51 (47%) Frame = +3 Query: 69 NSARGXT*XYFQANYLMVFLGLFAYCLITSPLLLIAMVASFFGYRKLTSGP 221 NS G + FQ+ + ++F GL +I S + LI+ FG K P Sbjct: 52 NSKLGLSFGVFQSVFSLLFSGLHKLWMILSRVPLISTFIPIFGTTKRVLSP 102 >SPAC11E3.05 |||ubiquitin-protein ligase E3|Schizosaccharomyces pombe|chr 1|||Manual Length = 1323 Score = 26.6 bits (56), Expect = 9.5 Identities = 13/26 (50%), Positives = 15/26 (57%) Frame = -1 Query: 156 MLSNSKQINPETPLNNWLENIXMXPL 79 M ++K NP TPL NWL I M L Sbjct: 634 MTRSTKSTNPMTPL-NWLRGISMGRL 658 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 4,512,585 Number of Sequences: 5004 Number of extensions: 84390 Number of successful extensions: 178 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 175 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 177 length of database: 2,362,478 effective HSP length: 76 effective length of database: 1,982,174 effective search space used: 897924822 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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