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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 030723E4_F06_e334_12.seq
         (1590 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC306.02c |||Rab GTPase binding |Schizosaccharomyces pombe|chr...    46   1e-05
SPBC543.07 |pek1|skh1, mkk1|MAP kinase kinase Pek1 |Schizosaccha...    29   1.8  
SPBC26H8.03 |cho2||phosphatidylethanolamine N-methyltransferase ...    27   7.2  
SPBP35G2.10 |mit1||SHREC complex subunit Mit1|Schizosaccharomyce...    27   7.2  
SPCC285.11 |ucp10||UBA/UAS domain protein Ucp10|Schizosaccharomy...    27   9.5  
SPAC11E3.05 |||ubiquitin-protein ligase E3|Schizosaccharomyces p...    27   9.5  

>SPCC306.02c |||Rab GTPase binding |Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 171

 Score = 46.0 bits (104), Expect = 1e-05
 Identities = 29/99 (29%), Positives = 47/99 (47%), Gaps = 3/99 (3%)
 Frame = +3

Query: 99  FQANYLMVFLGLFAYCLITSPLLLIAM---VASFFGYRKLTSGPNTWKIGSWELTKTQQY 269
           F +NYL +   L  Y LI +PLLLI +   V   +G RKL       ++    ++    Y
Sbjct: 61  FSSNYLAIIAMLVIYALIRNPLLLIVIGIGVGGVYGIRKLQGA--DLQLSHRVISNQNLY 118

Query: 270 AVAAAGSMGICWLAGAGAVLFWVLGATVTVVALHASFFD 386
            + A   + +   A     + W++GA+   V  HA+FF+
Sbjct: 119 VILACVLIPLGLFASPIETIIWLVGASCVCVFGHAAFFE 157


>SPBC543.07 |pek1|skh1, mkk1|MAP kinase kinase Pek1
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 363

 Score = 29.1 bits (62), Expect = 1.8
 Identities = 17/50 (34%), Positives = 23/50 (46%)
 Frame = +3

Query: 333 WVLGATVTVVALHASFFDAESLPPSDDPEQFPMMEQV*HSPLPLFMPPPG 482
           W LG T+  VAL+   F  E  PP   P    ++  + + P PL    PG
Sbjct: 263 WSLGLTLMEVALNRFPFPPEGSPP---PMPIELLSYIINMPPPLLPQEPG 309


>SPBC26H8.03 |cho2||phosphatidylethanolamine N-methyltransferase
           Cho2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 905

 Score = 27.1 bits (57), Expect = 7.2
 Identities = 14/32 (43%), Positives = 18/32 (56%)
 Frame = +3

Query: 237 GSWELTKTQQYAVAAAGSMGICWLAGAGAVLF 332
           G +EL     Y+V  AG  G+  L G+ AVLF
Sbjct: 287 GVFELAPHPMYSVGYAGYYGMSLLTGSYAVLF 318


>SPBP35G2.10 |mit1||SHREC complex subunit Mit1|Schizosaccharomyces
            pombe|chr 2|||Manual
          Length = 1418

 Score = 27.1 bits (57), Expect = 7.2
 Identities = 10/29 (34%), Positives = 17/29 (58%), Gaps = 1/29 (3%)
 Frame = +1

Query: 445  DTVPCRC-SCRHLGVYCRSTVSTLKLCII 528
            D V CRC   +HL  +C  ++  L++C +
Sbjct: 1307 DNVRCRCCGIKHLPAHCPLSIVPLEICFL 1335


>SPCC285.11 |ucp10||UBA/UAS domain protein Ucp10|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 427

 Score = 26.6 bits (56), Expect = 9.5
 Identities = 16/51 (31%), Positives = 24/51 (47%)
 Frame = +3

Query: 69  NSARGXT*XYFQANYLMVFLGLFAYCLITSPLLLIAMVASFFGYRKLTSGP 221
           NS  G +   FQ+ + ++F GL    +I S + LI+     FG  K    P
Sbjct: 52  NSKLGLSFGVFQSVFSLLFSGLHKLWMILSRVPLISTFIPIFGTTKRVLSP 102


>SPAC11E3.05 |||ubiquitin-protein ligase E3|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1323

 Score = 26.6 bits (56), Expect = 9.5
 Identities = 13/26 (50%), Positives = 15/26 (57%)
 Frame = -1

Query: 156 MLSNSKQINPETPLNNWLENIXMXPL 79
           M  ++K  NP TPL NWL  I M  L
Sbjct: 634 MTRSTKSTNPMTPL-NWLRGISMGRL 658


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 4,512,585
Number of Sequences: 5004
Number of extensions: 84390
Number of successful extensions: 178
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 175
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 177
length of database: 2,362,478
effective HSP length: 76
effective length of database: 1,982,174
effective search space used: 897924822
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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