Samia BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 030723E4_E12_e381_10.seq
         (1548 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC20G4.06c |adf1|cof1|cofilin|Schizosaccharomyces pombe|chr 1|...   101   2e-22
SPCC126.06 |twf1||twinfilin|Schizosaccharomyces pombe|chr 3|||Ma...    36   0.020
SPAC17H9.11 |||cofilin/tropomyosin family protein|Schizosaccharo...    29   1.3  
SPAC29A4.11 |rga3||GTPase activating protein Rga3|Schizosaccharo...    28   3.0  
SPBC20F10.03 |||conserved eukaryotic protein|Schizosaccharomyces...    28   4.0  
SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase Lkh1|Schi...    28   4.0  
SPBP35G2.03c |sgo1||shugoshin Sgo1|Schizosaccharomyces pombe|chr...    27   5.3  
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M...    27   5.3  
SPAC1093.03 |||inositol polyphosphate phosphatase |Schizosacchar...    27   5.3  
SPCC1450.04 |tef5||translation elongation factor EF-1 beta subun...    27   7.0  

>SPAC20G4.06c |adf1|cof1|cofilin|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 137

 Score =  101 bits (243), Expect = 2e-22
 Identities = 56/140 (40%), Positives = 82/140 (58%)
 Frame = +3

Query: 141 SGVTVSDACKTTYEEIKKDKKHRYVVFYIRDEKQIDVETVGGRNAEYDSFLEDLQKGGTG 320
           SGV VS  C   ++E+K  K  RYVVF + D K   V      + ++D+FL DL +    
Sbjct: 4   SGVKVSPECLEAFQELKLGKSLRYVVFKMNDTKTEIVVEKKSTDKDFDTFLGDLPEK--- 60

Query: 321 ECRYGLFDFEYTHQCQGTSEASKKQKLFLMSWCPDTAKVKKKMLYSSSFDALKKSLVGVQ 500
           +CRY ++DFE+ +  +G      + K+  +SW PD A +K KM+YSSS D L+++  G+ 
Sbjct: 61  DCRYAIYDFEF-NLGEGV-----RNKIIFISWSPDVAPIKSKMVYSSSKDTLRRAFTGIG 114

Query: 501 KYIQATDLSEASQEAVEEKL 560
             IQATD SE + E V EK+
Sbjct: 115 TDIQATDFSEVAYETVLEKV 134


>SPCC126.06 |twf1||twinfilin|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 328

 Score = 35.5 bits (78), Expect = 0.020
 Identities = 27/86 (31%), Positives = 44/86 (51%)
 Frame = +3

Query: 207 RYVVFYIRDEKQIDVETVGGRNAEYDSFLEDLQKGGTGECRYGLFDFEYTHQCQGTSEAS 386
           R  +  I +E   DV+T+  ++   +S   D +K    EC  G  +  +        + S
Sbjct: 26  RAAIISISNENSFDVKTMVEKSESIES---DFKK--VRECLLGSEEPAFVL----VYDDS 76

Query: 387 KKQKLFLMSWCPDTAKVKKKMLYSSS 464
           KK  L L+S+ P+ A V++KMLY+SS
Sbjct: 77  KKNLLQLISYVPENANVRRKMLYASS 102



 Score = 28.7 bits (61), Expect = 2.3
 Identities = 16/56 (28%), Positives = 32/56 (57%), Gaps = 5/56 (8%)
 Frame = +3

Query: 396 KLFLMSWCPDTAKVKKKMLYSSS----FDALKKSL-VGVQKYIQATDLSEASQEAV 548
           K+  +  CP  A VK +M+YSSS     D++K  L + +   I++ D ++ +++ +
Sbjct: 244 KILFIYICPMQATVKHRMVYSSSKLGLLDSIKAELGIVIDGKIESNDAADITEKEI 299


>SPAC17H9.11 |||cofilin/tropomyosin family
           protein|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 141

 Score = 29.5 bits (63), Expect = 1.3
 Identities = 17/63 (26%), Positives = 30/63 (47%)
 Frame = +3

Query: 372 TSEASKKQKLFLMSWCPDTAKVKKKMLYSSSFDALKKSLVGVQKYIQATDLSEASQEAVE 551
           T++      LF++ W P        M+Y+S+     + +  V K  +A D  + + EAV+
Sbjct: 79  TTDGRLSTPLFMIYWRPSATPNDLSMIYASA-KVWFQDVSQVHKVFEARDSEDITSEAVD 137

Query: 552 EKL 560
           E L
Sbjct: 138 EFL 140


>SPAC29A4.11 |rga3||GTPase activating protein
           Rga3|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 969

 Score = 28.3 bits (60), Expect = 3.0
 Identities = 17/55 (30%), Positives = 21/55 (38%)
 Frame = +2

Query: 650 PLSHTVVRDRRPPIHSNGRRPSAHAQTDAAXRFTPTGRRPPVHSSTGGVPYYTTN 814
           P     + DR  PI  NG   S H  T     F+P+ R    HS     P   T+
Sbjct: 323 PYKTMSLTDRAEPIVMNGHMRSLHNATSPFRPFSPSYRSSDTHSPRTRSPNVQTH 377


>SPBC20F10.03 |||conserved eukaryotic protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 446

 Score = 27.9 bits (59), Expect = 4.0
 Identities = 14/42 (33%), Positives = 23/42 (54%)
 Frame = -2

Query: 461 ARVQHLLLDLGGVWAPRH*EQFLFLARLRCTLALMRVFKIEE 336
           A ++ LLL +     PR  ++ +   R+ CT  L  VF++EE
Sbjct: 93  ASLEELLLKIFN--GPRSDKELILTIRIMCTFCLASVFQVEE 132


>SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase
           Lkh1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 690

 Score = 27.9 bits (59), Expect = 4.0
 Identities = 16/54 (29%), Positives = 25/54 (46%)
 Frame = -3

Query: 415 HDIRNNFCFLLASDVPWH*CVYSKSKRPYLHSPVPPFCRSSRKLSYSALRPPTV 254
           HD+ ++  +++ S   +H C  ++S  P  HS  PP            LRPP V
Sbjct: 107 HDVASHPSYMVQSPTSYHACSNNQSPFP--HSHHPPLHNPLPVSCQPVLRPPPV 158


>SPBP35G2.03c |sgo1||shugoshin Sgo1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 319

 Score = 27.5 bits (58), Expect = 5.3
 Identities = 27/136 (19%), Positives = 61/136 (44%), Gaps = 1/136 (0%)
 Frame = +3

Query: 168 KTTYEEIKKDKKHRYVVFYIRDEKQIDVETVGGRNAEYDSFLEDLQKGGTGECRYGLFDF 347
           K + + ++ + K ++     +D K+ D + +  +  EY SF ++L+   TG+  Y   DF
Sbjct: 108 KCSSDFLEANVKSQFTTCENKDSKE-DYQILHNKRLEYVSFNDELKSLETGQPLYCFQDF 166

Query: 348 EYTHQCQGTSEASKKQKLFLMSWCPDTAKVKKKMLYSSSFDALKKSLVGVQKYIQATDLS 527
           +   +  G    S+K    ++      A V K      ++   +K++    K ++  +  
Sbjct: 167 Q--KKVHGPPALSEKPGKCILK-DKTNAHVNKIPQDEVNYSLPQKNITIFSKELKENEFE 223

Query: 528 EASQ-EAVEEKLRATD 572
             ++ E  EEK + ++
Sbjct: 224 SINEGETEEEKAKTSN 239


>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 309

 Score = 27.5 bits (58), Expect = 5.3
 Identities = 24/86 (27%), Positives = 34/86 (39%), Gaps = 4/86 (4%)
 Frame = +2

Query: 527 GGVAGGRRGETPRH*PSVNPMHLALPSTPPSHT---RYERRVRGPLSHTVVRDRRPPIHS 697
           GGV G R+  TP+   +  P+    PS P S     + E R   P    +     P +  
Sbjct: 56  GGVVGERKSNTPKS-FAAPPVPTGAPSLPTSSNNTQQAEERPSMPALGGLFAGGMPKLRH 114

Query: 698 NGRRPSAHAQTDAAXRFTPTGR-RPP 772
            G+  ++ A   A    TP    RPP
Sbjct: 115 IGKSSASAAPPSAPAPPTPQSELRPP 140


>SPAC1093.03 |||inositol polyphosphate phosphatase
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 832

 Score = 27.5 bits (58), Expect = 5.3
 Identities = 11/25 (44%), Positives = 18/25 (72%)
 Frame = +2

Query: 770 PVHSSTGGVPYYTTNVSVYRGPLPI 844
           P+ S+T G+P YT+ V  +RG +P+
Sbjct: 271 PISSTTPGIPCYTSYVQ-HRGSIPL 294


>SPCC1450.04 |tef5||translation elongation factor EF-1 beta subunit
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 214

 Score = 27.1 bits (57), Expect = 7.0
 Identities = 10/26 (38%), Positives = 19/26 (73%)
 Frame = +3

Query: 462 SFDALKKSLVGVQKYIQATDLSEASQ 539
           S +AL++ L G + Y+Q+TD++  S+
Sbjct: 188 SLEALQEELEGFEDYVQSTDIAAMSK 213


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 4,705,642
Number of Sequences: 5004
Number of extensions: 87222
Number of successful extensions: 278
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 261
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 273
length of database: 2,362,478
effective HSP length: 76
effective length of database: 1,982,174
effective search space used: 870174386
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)