Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030723E4_E08_e349_10.seq
(1528 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_Q9VKC5 Cluster: CG6770-PA; n=9; Arthropoda|Rep: CG6770-... 122 3e-26
UniRef50_A6YPH3 Cluster: DNA-binding nuclear protein p8; n=3; Pa... 110 8e-23
UniRef50_Q6WLC3 Cluster: P8 protein; n=2; Coelomata|Rep: P8 prot... 71 8e-11
UniRef50_A7T1V1 Cluster: Predicted protein; n=1; Nematostella ve... 69 3e-10
UniRef50_Q9WTK0 Cluster: Nuclear protein 1; n=7; Theria|Rep: Nuc... 57 1e-06
UniRef50_O60356 Cluster: Nuclear protein 1; n=5; Catarrhini|Rep:... 56 2e-06
UniRef50_Q98TC8 Cluster: XP8; n=5; Eumetazoa|Rep: XP8 - Xenopus ... 55 4e-06
UniRef50_UPI0000498F17 Cluster: beta-galactosidase; n=3; Eukaryo... 53 2e-05
UniRef50_Q37953 Cluster: LacZ protein; n=1; Phage M13mp18|Rep: L... 49 3e-04
UniRef50_P00722 Cluster: Beta-galactosidase; n=35; root|Rep: Bet... 49 3e-04
UniRef50_UPI0001555AE4 Cluster: PREDICTED: similar to Gene produ... 49 4e-04
UniRef50_Q8GEG0 Cluster: Putative uncharacterized protein; n=1; ... 47 0.001
UniRef50_UPI0000F2C4D2 Cluster: PREDICTED: similar to 4930579G22... 46 0.002
UniRef50_Q47336 Cluster: LacZ-alpha peptide; n=2; cellular organ... 43 0.024
UniRef50_A6NF83 Cluster: Uncharacterized protein ENSP00000370281... 40 0.17
UniRef50_UPI0000E46543 Cluster: PREDICTED: hypothetical protein;... 39 0.30
UniRef50_Q4Z0C1 Cluster: Putative uncharacterized protein; n=3; ... 37 1.2
UniRef50_UPI000155F4F8 Cluster: PREDICTED: hypothetical protein;... 35 6.5
UniRef50_Q5BQT9 Cluster: SJCHGC09812 protein; n=1; Schistosoma j... 35 6.5
>UniRef50_Q9VKC5 Cluster: CG6770-PA; n=9; Arthropoda|Rep: CG6770-PA
- Drosophila melanogaster (Fruit fly)
Length = 69
Score = 122 bits (293), Expect = 3e-26
Identities = 51/62 (82%), Positives = 56/62 (90%)
Frame = +1
Query: 142 MSEAFFDEYDYYNFDHDKHIFTGHGGKQRTKREATEHTNHFDPSGHSRKIVTKLVNTENN 321
MSEA FDEY++YNFDHDKHIF+GH GKQR KREA EHTNHFDPSGHSRKI+TKL+NT NN
Sbjct: 1 MSEAHFDEYEHYNFDHDKHIFSGHSGKQRNKREANEHTNHFDPSGHSRKILTKLMNTNNN 60
Query: 322 KK 327
K
Sbjct: 61 NK 62
>UniRef50_A6YPH3 Cluster: DNA-binding nuclear protein p8; n=3;
Pancrustacea|Rep: DNA-binding nuclear protein p8 -
Triatoma infestans (Assassin bug)
Length = 73
Score = 110 bits (265), Expect = 8e-23
Identities = 46/62 (74%), Positives = 55/62 (88%)
Frame = +1
Query: 142 MSEAFFDEYDYYNFDHDKHIFTGHGGKQRTKREATEHTNHFDPSGHSRKIVTKLVNTENN 321
MSE+ FDEY++YNFDHDKH+++G GKQR+K+EA+EHTNHFDPSGHSRKIVTKL NTE
Sbjct: 1 MSESHFDEYEHYNFDHDKHMYSGASGKQRSKKEASEHTNHFDPSGHSRKIVTKLQNTEKK 60
Query: 322 KK 327
K
Sbjct: 61 TK 62
>UniRef50_Q6WLC3 Cluster: P8 protein; n=2; Coelomata|Rep: P8 protein
- Branchiostoma belcheri tsingtauense
Length = 79
Score = 70.9 bits (166), Expect = 8e-11
Identities = 32/61 (52%), Positives = 42/61 (68%), Gaps = 1/61 (1%)
Frame = +1
Query: 154 FFDEYDYYNFDHDKHIFTGHG-GKQRTKREATEHTNHFDPSGHSRKIVTKLVNTENNKKS 330
++DEY+YYNFD DK + +G G GK RTKRE H+ FDP G+ R VTK NTE +K+
Sbjct: 15 YYDEYEYYNFDTDKIVASGQGSGKGRTKREQELHSTRFDPCGNVRITVTKFQNTERKRKN 74
Query: 331 N 333
+
Sbjct: 75 S 75
>UniRef50_A7T1V1 Cluster: Predicted protein; n=1; Nematostella
vectensis|Rep: Predicted protein - Nematostella
vectensis
Length = 66
Score = 68.9 bits (161), Expect = 3e-10
Identities = 31/59 (52%), Positives = 41/59 (69%)
Frame = +1
Query: 148 EAFFDEYDYYNFDHDKHIFTGHGGKQRTKREATEHTNHFDPSGHSRKIVTKLVNTENNK 324
E +FDEYDYYNFD +++ G+ K R+KREA +TN F P GH RK++ K NTE N+
Sbjct: 2 EPYFDEYDYYNFD--RNVVEGNTRKGRSKREACLNTNRFCPGGHERKVLEKYRNTEKNR 58
>UniRef50_Q9WTK0 Cluster: Nuclear protein 1; n=7; Theria|Rep:
Nuclear protein 1 - Mus musculus (Mouse)
Length = 80
Score = 57.2 bits (132), Expect = 1e-06
Identities = 28/64 (43%), Positives = 35/64 (54%)
Frame = +1
Query: 133 NVTMSEAFFDEYDYYNFDHDKHIFTGHGGKQRTKREATEHTNHFDPSGHSRKIVTKLVNT 312
N+ + DEYD Y+ H G G K RTKREA +TN P GH RK++TK N+
Sbjct: 16 NLEDEDGILDEYDQYSLAHP--CVVGGGRKGRTKREAAANTNRPSPGGHERKLLTKFQNS 73
Query: 313 ENNK 324
E K
Sbjct: 74 ERKK 77
>UniRef50_O60356 Cluster: Nuclear protein 1; n=5; Catarrhini|Rep:
Nuclear protein 1 - Homo sapiens (Human)
Length = 82
Score = 56.0 bits (129), Expect = 2e-06
Identities = 28/65 (43%), Positives = 40/65 (61%), Gaps = 1/65 (1%)
Frame = +1
Query: 148 EAFFDEYDYYNFDHDKHIFTGHGGKQ-RTKREATEHTNHFDPSGHSRKIVTKLVNTENNK 324
++ DE D Y+ H + G GG++ RTKREA +TN P GH RK+VTKL N+E K
Sbjct: 21 DSSLDESDLYSLAHS---YLGGGGRKGRTKREAAANTNRPSPGGHERKLVTKLQNSERKK 77
Query: 325 KSNKQ 339
+ ++
Sbjct: 78 RGARR 82
>UniRef50_Q98TC8 Cluster: XP8; n=5; Eumetazoa|Rep: XP8 - Xenopus
laevis (African clawed frog)
Length = 82
Score = 55.2 bits (127), Expect = 4e-06
Identities = 27/58 (46%), Positives = 35/58 (60%)
Frame = +1
Query: 154 FFDEYDYYNFDHDKHIFTGHGGKQRTKREATEHTNHFDPSGHSRKIVTKLVNTENNKK 327
+FDEY+YYN D++ K RTK+EA +TN P GH RKI +KL +E KK
Sbjct: 20 YFDEYEYYNLT-DRYSLPTAARKGRTKKEAEANTNRESPCGHERKISSKLQRSECKKK 76
>UniRef50_UPI0000498F17 Cluster: beta-galactosidase; n=3;
Eukaryota|Rep: beta-galactosidase - Entamoeba histolytica
HM-1:IMSS
Length = 86
Score = 52.8 bits (121), Expect = 2e-05
Identities = 29/68 (42%), Positives = 32/68 (47%), Gaps = 1/68 (1%)
Frame = +1
Query: 838 HWPAFYNVVTGKTLAYPTXSPCSTFPFRQL-X**RXGPTDRXSQQFAQPXWXMANWXRYI 1014
HWP+FYNVVTGKTLA P P TDR SQQ W MA RY
Sbjct: 5 HWPSFYNVVTGKTLALPNLIALQHIPLSPAGVISEEARTDRPSQQLRSLKWRMAPEWRYF 64
Query: 1015 LLKXXLNF 1038
LL + +
Sbjct: 65 LLTQSVRY 72
>UniRef50_Q37953 Cluster: LacZ protein; n=1; Phage M13mp18|Rep: LacZ
protein - Phage M13mp18
Length = 102
Score = 49.2 bits (112), Expect = 3e-04
Identities = 24/41 (58%), Positives = 25/41 (60%)
Frame = +2
Query: 875 PWRTQLXRLAAHSPFASWXNSEXAPPIAXPNSLRSLXGXWQ 997
P TQL RLAAH PFASW NSE A LRSL G W+
Sbjct: 38 PGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWR 78
Score = 35.9 bits (79), Expect = 2.8
Identities = 17/25 (68%), Positives = 17/25 (68%)
Frame = +3
Query: 840 LAGVLQRRDWENPGVPNLXALQHIP 914
LA VLQRRDWENPGV L L P
Sbjct: 26 LAVVLQRRDWENPGVTQLNRLAAHP 50
>UniRef50_P00722 Cluster: Beta-galactosidase; n=35; root|Rep:
Beta-galactosidase - Escherichia coli (strain K12)
Length = 1024
Score = 49.2 bits (112), Expect = 3e-04
Identities = 24/41 (58%), Positives = 25/41 (60%)
Frame = +2
Query: 875 PWRTQLXRLAAHSPFASWXNSEXAPPIAXPNSLRSLXGXWQ 997
P TQL RLAAH PFASW NSE A LRSL G W+
Sbjct: 20 PGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWR 60
Score = 35.9 bits (79), Expect = 2.8
Identities = 17/25 (68%), Positives = 17/25 (68%)
Frame = +3
Query: 840 LAGVLQRRDWENPGVPNLXALQHIP 914
LA VLQRRDWENPGV L L P
Sbjct: 8 LAVVLQRRDWENPGVTQLNRLAAHP 32
>UniRef50_UPI0001555AE4 Cluster: PREDICTED: similar to Gene product
with similarity to Rat P8; n=1; Ornithorhynchus
anatinus|Rep: PREDICTED: similar to Gene product with
similarity to Rat P8 - Ornithorhynchus anatinus
Length = 176
Score = 48.8 bits (111), Expect = 4e-04
Identities = 22/43 (51%), Positives = 30/43 (69%), Gaps = 1/43 (2%)
Frame = +1
Query: 202 FTGHGGKQ-RTKREATEHTNHFDPSGHSRKIVTKLVNTENNKK 327
F+G G ++ R+K+EA HTN P GH RK++TKL NTE K+
Sbjct: 130 FSGGGARKGRSKKEAAIHTNRPSPGGHERKLLTKLQNTEKKKQ 172
>UniRef50_Q8GEG0 Cluster: Putative uncharacterized protein; n=1;
Erwinia amylovora|Rep: Putative uncharacterized protein
- Erwinia amylovora (Fire blight bacteria)
Length = 123
Score = 47.2 bits (107), Expect = 0.001
Identities = 23/41 (56%), Positives = 24/41 (58%)
Frame = +2
Query: 875 PWRTQLXRLAAHSPFASWXNSEXAPPIAXPNSLRSLXGXWQ 997
P TQL RLAAH PFASW NSE A LR L G W+
Sbjct: 80 PGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRXLNGEWR 120
Score = 35.9 bits (79), Expect = 2.8
Identities = 17/25 (68%), Positives = 17/25 (68%)
Frame = +3
Query: 840 LAGVLQRRDWENPGVPNLXALQHIP 914
LA VLQRRDWENPGV L L P
Sbjct: 68 LAVVLQRRDWENPGVTQLNRLAAHP 92
>UniRef50_UPI0000F2C4D2 Cluster: PREDICTED: similar to 4930579G22Rik
protein; n=1; Monodelphis domestica|Rep: PREDICTED:
similar to 4930579G22Rik protein - Monodelphis domestica
Length = 112
Score = 46.4 bits (105), Expect = 0.002
Identities = 25/64 (39%), Positives = 35/64 (54%), Gaps = 1/64 (1%)
Frame = +1
Query: 148 EAFFDEYDYYNFDHDKHIFTGHG-GKQRTKREATEHTNHFDPSGHSRKIVTKLVNTENNK 324
E +D YDYYN ++ G G K RT+RE TN P+GH RKI KL N++ +
Sbjct: 41 EEHYDCYDYYNLR--EYPIRGPGWSKGRTRRERELRTNRPVPAGHERKIAQKLYNSQRKR 98
Query: 325 KSNK 336
+ +
Sbjct: 99 RQRQ 102
>UniRef50_Q47336 Cluster: LacZ-alpha peptide; n=2; cellular
organisms|Rep: LacZ-alpha peptide - Escherichia coli
Length = 90
Score = 42.7 bits (96), Expect = 0.024
Identities = 22/36 (61%), Positives = 22/36 (61%)
Frame = +2
Query: 875 PWRTQLXRLAAHSPFASWXNSEXAPPIAXPNSLRSL 982
P TQL RLAAH PFASW NSE A LRSL
Sbjct: 34 PGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSL 69
Score = 35.9 bits (79), Expect = 2.8
Identities = 17/25 (68%), Positives = 17/25 (68%)
Frame = +3
Query: 840 LAGVLQRRDWENPGVPNLXALQHIP 914
LA VLQRRDWENPGV L L P
Sbjct: 22 LAVVLQRRDWENPGVTQLNRLAAHP 46
>UniRef50_A6NF83 Cluster: Uncharacterized protein ENSP00000370281;
n=11; Eutheria|Rep: Uncharacterized protein
ENSP00000370281 - Homo sapiens (Human)
Length = 97
Score = 39.9 bits (89), Expect = 0.17
Identities = 21/67 (31%), Positives = 31/67 (46%)
Frame = +1
Query: 136 VTMSEAFFDEYDYYNFDHDKHIFTGHGGKQRTKREATEHTNHFDPSGHSRKIVTKLVNTE 315
++ E +D DYY G K RT+RE TN P GH RK+ KL+N +
Sbjct: 22 ISYEEELYDCLDYYYLRDFPACGAGRS-KGRTRREQALRTNWPAPGGHERKVAQKLLNGQ 80
Query: 316 NNKKSNK 336
++ +
Sbjct: 81 RKRRQRQ 87
>UniRef50_UPI0000E46543 Cluster: PREDICTED: hypothetical protein;
n=1; Strongylocentrotus purpuratus|Rep: PREDICTED:
hypothetical protein - Strongylocentrotus purpuratus
Length = 70
Score = 39.1 bits (87), Expect = 0.30
Identities = 22/63 (34%), Positives = 37/63 (58%), Gaps = 1/63 (1%)
Frame = +1
Query: 142 MSEAFFDEYDYYNFDHDKHIFTGHGGKQRTKRE-ATEHTNHFDPSGHSRKIVTKLVNTEN 318
MSE FD+ ++YN+D I +G GK +K+E AT+ N+ + +R + ++ N+E
Sbjct: 1 MSEDHFDDLEHYNYD---PIHSGASGKGLSKKEKATKEHNNPHTTKEARLVEDQITNSEQ 57
Query: 319 NKK 327
KK
Sbjct: 58 KKK 60
>UniRef50_Q4Z0C1 Cluster: Putative uncharacterized protein; n=3;
Plasmodium (Vinckeia)|Rep: Putative uncharacterized
protein - Plasmodium berghei
Length = 275
Score = 37.1 bits (82), Expect = 1.2
Identities = 16/16 (100%), Positives = 16/16 (100%)
Frame = +1
Query: 787 RGGARYPIRPIVSRIT 834
RGGARYPIRPIVSRIT
Sbjct: 260 RGGARYPIRPIVSRIT 275
>UniRef50_UPI000155F4F8 Cluster: PREDICTED: hypothetical protein;
n=1; Equus caballus|Rep: PREDICTED: hypothetical protein
- Equus caballus
Length = 197
Score = 34.7 bits (76), Expect = 6.5
Identities = 16/39 (41%), Positives = 22/39 (56%)
Frame = +1
Query: 220 KQRTKREATEHTNHFDPSGHSRKIVTKLVNTENNKKSNK 336
K R++RE TN P GH RKI KLVN + ++ +
Sbjct: 149 KGRSRRERELRTNRPVPVGHERKIAQKLVNGQRKRRQRQ 187
>UniRef50_Q5BQT9 Cluster: SJCHGC09812 protein; n=1; Schistosoma
japonicum|Rep: SJCHGC09812 protein - Schistosoma
japonicum (Blood fluke)
Length = 62
Score = 34.7 bits (76), Expect = 6.5
Identities = 20/52 (38%), Positives = 29/52 (55%), Gaps = 1/52 (1%)
Frame = +1
Query: 175 YNFDHDKHIFTGHGGKQRTKREATEHTNHFDPSGHSRKI-VTKLVNTENNKK 327
YN++ DK I G GGK RTK++A +H H + +I V VN E ++
Sbjct: 2 YNYEEDK-IAGGKGGKNRTKKDAEDH--HKKSENRTNRIHVDHQVNNEEKQR 50
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,074,099,610
Number of Sequences: 1657284
Number of extensions: 18875122
Number of successful extensions: 40215
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 38612
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 40179
length of database: 575,637,011
effective HSP length: 104
effective length of database: 403,279,475
effective search space used: 162924907900
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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