Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030723E4_E02_e301_10.seq
(1558 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g03190.1 68416.m00315 glutathione S-transferase, putative ide... 40 0.003
At5g17220.1 68418.m02018 glutathione S-transferase, putative 38 0.014
At1g57720.1 68414.m06549 elongation factor 1B-gamma, putative / ... 38 0.018
At2g30870.1 68415.m03762 glutathione S-transferase, putative sup... 37 0.041
At1g09640.1 68414.m01081 elongation factor 1B-gamma, putative / ... 33 0.51
At3g62760.1 68416.m07050 glutathione S-transferase, putative Glu... 31 1.6
At1g49860.1 68414.m05590 glutathione S-transferase, putative sim... 31 1.6
At1g49660.1 68414.m05569 expressed protein 31 1.6
At4g02520.1 68417.m00345 glutathione S-transferase, putative 30 3.6
At2g47730.1 68415.m05960 glutathione S-transferase 6 (GST6) iden... 30 3.6
At2g02930.1 68415.m00241 glutathione S-transferase, putative 30 3.6
At5g53460.1 68418.m06644 glutamate synthase [NADH], chloroplast,... 30 4.7
At2g20980.1 68415.m02483 expressed protein 30 4.7
>At3g03190.1 68416.m00315 glutathione S-transferase, putative
identical to glutathione S-transferase GB:AAB09584 from
[Arabidopsis thaliana]
Length = 214
Score = 40.3 bits (90), Expect = 0.003
Identities = 30/85 (35%), Positives = 44/85 (51%), Gaps = 6/85 (7%)
Frame = +2
Query: 155 VVFYYFPVKALGEGPRMLLAYGGQ--EFEDKRISMDQWPEIKP----KTPFGQMPLLEID 316
VV Y +KA R+LL + + EFE + +D+ + KP + PFGQ+P +E
Sbjct: 2 VVKVYGQIKAANP-QRVLLCFLEKDIEFEVIHVDLDKLEQKKPQHLLRQPFGQVPAIEDG 60
Query: 317 GKQYAQSIAISRYLGRKYGLAGADL 391
+ +S AI+RY KY G DL
Sbjct: 61 YLKLFESRAIARYYATKYADQGTDL 85
>At5g17220.1 68418.m02018 glutathione S-transferase, putative
Length = 214
Score = 38.3 bits (85), Expect = 0.014
Identities = 25/70 (35%), Positives = 38/70 (54%), Gaps = 6/70 (8%)
Frame = +2
Query: 200 RMLLAY--GGQEFEDKRISMDQWPEIKP----KTPFGQMPLLEIDGKQYAQSIAISRYLG 361
R+LL + G EFE I +D + + KP + PFGQ+P +E + +S AI+RY
Sbjct: 16 RVLLCFLEKGIEFEIIHIDLDTFEQKKPEHLLRQPFGQVPAIEDGDFKLFESRAIARYYA 75
Query: 362 RKYGLAGADL 391
K+ G +L
Sbjct: 76 TKFADQGTNL 85
>At1g57720.1 68414.m06549 elongation factor 1B-gamma, putative /
eEF-1B gamma, putative similar to elongation factor 1B
gamma GI:3868758 from [Oryza sativa]
Length = 413
Score = 37.9 bits (84), Expect = 0.018
Identities = 25/66 (37%), Positives = 37/66 (56%), Gaps = 4/66 (6%)
Frame = +2
Query: 263 PEIKPKTPFGQMPLLEIDGKQYAQSIAISRYLGRKYG---LAGADLEEDFEIDQNVEFLN 433
PE P G++P+LE +S AI+RY+ RK G L G+ L E I+Q ++F +
Sbjct: 43 PEFLKMNPIGKVPVLETPEGPIFESNAIARYVSRKNGDNSLNGSSLIEYAHIEQWIDFSS 102
Query: 434 -DIRAN 448
+I AN
Sbjct: 103 LEIDAN 108
>At2g30870.1 68415.m03762 glutathione S-transferase, putative
supported by cDNA GI:443698 GB:D17673
Length = 215
Score = 36.7 bits (81), Expect = 0.041
Identities = 27/84 (32%), Positives = 41/84 (48%), Gaps = 5/84 (5%)
Frame = +2
Query: 155 VVFYYFPVKALGEGPRMLLAYGGQEFEDKRISM----DQWPEIKPKTPFGQMPLLEIDGK 322
V+ Y P+ A + + L G FE + + + PE PFG++P+L +DG
Sbjct: 2 VLTIYAPLFASSKRAVVTLVEKGVSFETVNVDLMKGEQRQPEYLAIQPFGKIPVL-VDGD 60
Query: 323 -QYAQSIAISRYLGRKYGLAGADL 391
+ +S AI RY+ KY G DL
Sbjct: 61 YKIFESRAIMRYIAEKYRSQGPDL 84
>At1g09640.1 68414.m01081 elongation factor 1B-gamma, putative /
eEF-1B gamma, putative Similar to elongation factor
1-gamma (gb|EF1G_XENLA). ESTs
gb|T20564,gb|T45940,gb|T04527 come from this gene
Length = 414
Score = 33.1 bits (72), Expect = 0.51
Identities = 20/58 (34%), Positives = 31/58 (53%), Gaps = 3/58 (5%)
Frame = +2
Query: 263 PEIKPKTPFGQMPLLEIDGKQYAQSIAISRYLGRKYG---LAGADLEEDFEIDQNVEF 427
P P G++P+LE +S AI+RY+ R G L G+ L E +I+Q ++F
Sbjct: 43 PAFLKMNPIGKVPVLETPEGSVFESNAIARYVSRLNGDNSLNGSSLIEYAQIEQWIDF 100
>At3g62760.1 68416.m07050 glutathione S-transferase, putative
Glutathione transferase III(b) - Zea mays, EMBL:AJ010296
Length = 219
Score = 31.5 bits (68), Expect = 1.6
Identities = 15/43 (34%), Positives = 22/43 (51%)
Frame = +2
Query: 263 PEIKPKTPFGQMPLLEIDGKQYAQSIAISRYLGRKYGLAGADL 391
P PFG++P L+ D +S AI+ Y+ K+ G DL
Sbjct: 43 PSFLSMNPFGKVPALQDDDLTLFESRAITAYIAEKHRDKGTDL 85
>At1g49860.1 68414.m05590 glutathione S-transferase, putative
similar to GI:860955 from [Hyoscyamus muticus] (Plant
Physiol. 109 (1), 253-260 (1995))
Length = 254
Score = 31.5 bits (68), Expect = 1.6
Identities = 15/39 (38%), Positives = 23/39 (58%)
Frame = +2
Query: 284 PFGQMPLLEIDGKQYAQSIAISRYLGRKYGLAGADLEED 400
PFG++P+LE + + AI+RYL +Y G +L D
Sbjct: 53 PFGEVPVLEDGDLKLFEPKAITRYLAEQYKDVGTNLLPD 91
>At1g49660.1 68414.m05569 expressed protein
Length = 319
Score = 31.5 bits (68), Expect = 1.6
Identities = 16/44 (36%), Positives = 26/44 (59%)
Frame = -1
Query: 667 LFKCRFLKHRLQVIEHTGEDEIAPSQLSKSDVAAVLLEYFIQFL 536
L KC + + ++V+E GED + Q KSD A L+ F++F+
Sbjct: 275 LEKCEW-EGTVEVVEEEGEDHVFHLQNPKSDKALKFLKKFVEFI 317
>At4g02520.1 68417.m00345 glutathione S-transferase, putative
Length = 212
Score = 30.3 bits (65), Expect = 3.6
Identities = 13/38 (34%), Positives = 22/38 (57%)
Frame = +2
Query: 278 KTPFGQMPLLEIDGKQYAQSIAISRYLGRKYGLAGADL 391
+ PFGQ+P E + +S AI++Y+ +Y G +L
Sbjct: 49 RNPFGQVPAFEDGDLKLFESRAITQYIAHRYENQGTNL 86
>At2g47730.1 68415.m05960 glutathione S-transferase 6 (GST6)
identical to GB:X95295. Based on identical cDNA hits,
the translation is now 40 AAs longer at the N-terminal,
and start of exon2 is also corrected.
Length = 263
Score = 30.3 bits (65), Expect = 3.6
Identities = 15/36 (41%), Positives = 20/36 (55%)
Frame = +2
Query: 284 PFGQMPLLEIDGKQYAQSIAISRYLGRKYGLAGADL 391
PFGQ+P LE +S AI++YL +Y G L
Sbjct: 99 PFGQIPALEDGDLTLFESRAITQYLAEEYSEKGEKL 134
>At2g02930.1 68415.m00241 glutathione S-transferase, putative
Length = 212
Score = 30.3 bits (65), Expect = 3.6
Identities = 13/38 (34%), Positives = 22/38 (57%)
Frame = +2
Query: 278 KTPFGQMPLLEIDGKQYAQSIAISRYLGRKYGLAGADL 391
+ PFGQ+P E + +S AI++Y+ +Y G +L
Sbjct: 49 RNPFGQVPAFEDGDLKLFESRAITQYIAHRYENQGTNL 86
>At5g53460.1 68418.m06644 glutamate synthase [NADH], chloroplast,
putative similar to SP|Q03460 Glutamate synthase [NADH],
chloroplast precursor (EC 1.4.1.14) (NADH- GOGAT)
{Medicago sativa}
Length = 2208
Score = 29.9 bits (64), Expect = 4.7
Identities = 17/52 (32%), Positives = 27/52 (51%), Gaps = 5/52 (9%)
Frame = +1
Query: 163 LLFPREGARRGSADVASLRRAGIRR-----QTHINGPMA*NQAQDPVRSNAV 303
L FP G GSA+ ++L G + H+N P+A + Q+ R+N+V
Sbjct: 915 LAFPTRGYAPGSAEASALTNPGNYHWRKNGEIHLNDPLAIAKLQEAARTNSV 966
>At2g20980.1 68415.m02483 expressed protein
Length = 396
Score = 29.9 bits (64), Expect = 4.7
Identities = 27/100 (27%), Positives = 45/100 (45%), Gaps = 4/100 (4%)
Frame = +2
Query: 320 KQYAQSIAISRYLGRKYGLAGADLEEDFEIDQNVEFLNDI-RANAAAVHYEKDEAVK--- 487
K+ Q + + G + L+GAD Q + FLN++ R A+ +K EAV
Sbjct: 291 KKTTQPVRVLSVEGLRKALSGADKVTPNVHSQGIRFLNEMARQKASKNVNKKSEAVNKST 350
Query: 488 AKKLAEFRETKYQFLIEKLNEILQKNGGHIALGKLTWGDF 607
K+ A +ET+ ++ +K I+ GK+ DF
Sbjct: 351 EKRKASTKETQVNGEPKRKKTEHRKETPEISTGKMMLLDF 390
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 21,044,046
Number of Sequences: 28952
Number of extensions: 391689
Number of successful extensions: 961
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 928
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 960
length of database: 12,070,560
effective HSP length: 84
effective length of database: 9,638,592
effective search space used: 4183148928
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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