BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 030723E4_D09_e356_07.seq
(1490 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1D4.14 |tho2|SPAC22F3.14c|THO complex subunit Tho2 |Schizosa... 29 2.2
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 28 3.8
SPAP11E10.01 |||ornithine cyclodeaminase family |Schizosaccharom... 27 5.0
SPCC70.04c |||sequence orphan|Schizosaccharomyces pombe|chr 3|||... 27 5.0
SPCC1739.06c |||uroporphyrin methyltransferase |Schizosaccharomy... 27 6.7
SPBC336.05c |||S-adenosylmethionine-dependentmethyltransferase|S... 27 6.7
>SPAC1D4.14 |tho2|SPAC22F3.14c|THO complex subunit Tho2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1628
Score = 28.7 bits (61), Expect = 2.2
Identities = 19/49 (38%), Positives = 26/49 (53%)
Frame = +3
Query: 696 QKAKRTNQSLYYSETKX*LKIDLVLEEETGYSELMVXSIFAFRDTCQRD 842
+K RTN SL Y + K +L+ EE G+S LM+ A TC R+
Sbjct: 152 RKIVRTNTSLLYRQKK----FNLLREESEGFSHLMINFFDAL--TCLRN 194
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 27.9 bits (59), Expect = 3.8
Identities = 13/44 (29%), Positives = 15/44 (34%)
Frame = +2
Query: 1358 GGXXPXXPXTXGPPPLGXXRPXXXGXXXPPPPGXAXXPPXXPXP 1489
GG P P + P P PPPP + PP P
Sbjct: 1679 GGMAPAHPVSTPPVRPQSAAPPQMSAPTPPPPPMSVPPPPSAPP 1722
Score = 27.5 bits (58), Expect = 5.0
Identities = 13/43 (30%), Positives = 15/43 (34%), Gaps = 3/43 (6%)
Frame = +2
Query: 1370 PXXPXTXGPPPLGXXRPXXXGXXXPPPPGX---AXXPPXXPXP 1489
P P + PP + P PPPP PP P P
Sbjct: 1691 PVRPQSAAPPQMSAPTPPPPPMSVPPPPSAPPMPAGPPSAPPP 1733
Score = 26.6 bits (56), Expect = 8.8
Identities = 14/40 (35%), Positives = 15/40 (37%)
Frame = +2
Query: 1370 PXXPXTXGPPPLGXXRPXXXGXXXPPPPGXAXXPPXXPXP 1489
P P + PPP P PPPP A P P P
Sbjct: 1708 PPPPMSVPPPPSAPPMP-AGPPSAPPPPLPASSAPSVPNP 1746
>SPAP11E10.01 |||ornithine cyclodeaminase family
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 330
Score = 27.5 bits (58), Expect = 5.0
Identities = 11/24 (45%), Positives = 15/24 (62%)
Frame = +3
Query: 384 ILIITSNKMIEDFLHIHCKVGVLR 455
+LII S K+ E +H HC V +R
Sbjct: 135 LLIIGSGKVAEKLIHAHCSVRPIR 158
>SPCC70.04c |||sequence orphan|Schizosaccharomyces pombe|chr
3|||Manual
Length = 244
Score = 27.5 bits (58), Expect = 5.0
Identities = 11/19 (57%), Positives = 14/19 (73%)
Frame = -2
Query: 166 FKYFYHLI*LTINYNYKIN 110
F F+HL+ LT NY+Y IN
Sbjct: 145 FGLFHHLMFLTTNYSYTIN 163
>SPCC1739.06c |||uroporphyrin methyltransferase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 496
Score = 27.1 bits (57), Expect = 6.7
Identities = 11/23 (47%), Positives = 14/23 (60%)
Frame = -1
Query: 572 YGHGGGEYIICYKHIYHNTIIQG 504
YG GG EY+ +H Y T+I G
Sbjct: 350 YGRGGEEYLFFTQHGYVPTVIPG 372
>SPBC336.05c |||S-adenosylmethionine-
dependentmethyltransferase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 378
Score = 27.1 bits (57), Expect = 6.7
Identities = 15/53 (28%), Positives = 26/53 (49%)
Frame = -2
Query: 664 VWEATVKIKHFFHLQTLLVSTITNLWEKSKRMDMAVVNILFVINIFTIILLSR 506
+WE++ I+H FH + + +KSK +A+ N F I ++ SR
Sbjct: 317 IWESSPLIRHAFHYDDSIYLSYCPELKKSKHKGIALAN-SFSFRIAKVLKKSR 368
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 4,478,590
Number of Sequences: 5004
Number of extensions: 80505
Number of successful extensions: 164
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 140
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 159
length of database: 2,362,478
effective HSP length: 76
effective length of database: 1,982,174
effective search space used: 832513080
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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