BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= 030723E4_D06_e332_08.seq (1553 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC3C7.11c |cnx1|cal1, cal1|calnexin |Schizosaccharomyces pombe... 86 1e-17 SPBP4H10.19c |||calreticulin/calnexin homolog|Schizosaccharomyce... 39 0.002 >SPAC3C7.11c |cnx1|cal1, cal1|calnexin |Schizosaccharomyces pombe|chr 1|||Manual Length = 560 Score = 85.8 bits (203), Expect = 1e-17 Identities = 51/139 (36%), Positives = 74/139 (53%), Gaps = 8/139 (5%) Frame = +3 Query: 264 EADKGLQTSEDARFYALSRKF-KPFSNEGKPLVVQFSVKHEQDIDCGGGYLKVFDCRLDQ 440 + + GL ++A +A+S +F +P + K LVVQ+ V E+ ++CGG YLK+ Sbjct: 87 KGEAGLVMKDEAAHHAISYEFDEPINEPEKDLVVQYEVNPEEGLNCGGAYLKLL-AEPTH 145 Query: 441 KDMHGETPYEIMFGPDICGPGTKKVHVIFSYK-------GKNHLIKKDIRCKDDVYTHLY 599 +M Y IMFGPD CG +VH IF +K + HL + T+LY Sbjct: 146 GEMSNSIDYRIMFGPDKCGV-NDRVHFIFKHKNPLTGEYSEKHLDSRPASLLKPGITNLY 204 Query: 600 TLIVKPDNTYEVLSDNEKV 656 TLIVKPD T+EV + + V Sbjct: 205 TLIVKPDQTFEVRINGDVV 223 Score = 29.9 bits (64), Expect = 0.99 Identities = 21/85 (24%), Positives = 32/85 (37%), Gaps = 1/85 (1%) Frame = +2 Query: 731 PED-LGPQTHXSPDPEDNETRXLGQTLEXHFGPLMXVSPEXLDDEXXXXXEPPYDXXPXL 907 PED L + PDPE + + + P ++P+ ++ +PP P Sbjct: 286 PEDWLEDEPLYIPDPEAQKPEDWDDEEDGDWIPSEIINPKCIEGAGCGEWKPPMIRNPNY 345 Query: 908 XGRLGPXQIXTPAXKGPWXHPXXXP 982 G P I P G W +P P Sbjct: 346 RGPWSPPMIPNPEFIGEW-YPRKIP 369 >SPBP4H10.19c |||calreticulin/calnexin homolog|Schizosaccharomyces pombe|chr 2|||Manual Length = 381 Score = 39.1 bits (87), Expect = 0.002 Identities = 48/178 (26%), Positives = 77/178 (43%), Gaps = 7/178 (3%) Frame = +3 Query: 144 VFFEEKFSDD--SWESNWVYSEHPGKEFGKFKLTAGKFYNDAEADKGLQTSEDARFYAL- 314 VF E+ S+D +W S W K+ G + L + + GL T + + + L Sbjct: 32 VFSEQFTSEDITTWRSRW--RAPVNKDLGVWDLVEAPG-SHLRDEYGLITLKSNKPHILI 88 Query: 315 SRKFKPFSNEGK--PLVVQFSVKHEQDIDCGGGYLKVFDCRLDQKDMHGETPYEIMFGPD 488 S P + + P+V+ F VK + CG Y+ + + + K++ E P I FG Sbjct: 89 SNLENPTTRQSSSVPIVLSFQVKPTKPWTCGHAYVSLVH-QSNPKNVSKEPPSVIRFGVK 147 Query: 489 ICGPGTKKVHVIFSYKGK--NHLIKKDIRCKDDVYTHLYTLIVKPDNTYEVLSDNEKV 656 CG I SY GK HL + T +YTL+++ NT V+ ++ V Sbjct: 148 KCGMFDYISLSIISYDGKVSCHLYDAPPSGLVEGRTSMYTLLLQ--NTTVVIRRDQSV 203 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 4,187,431 Number of Sequences: 5004 Number of extensions: 75086 Number of successful extensions: 206 Number of sequences better than 10.0: 2 Number of HSP's better than 10.0 without gapping: 197 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 201 length of database: 2,362,478 effective HSP length: 76 effective length of database: 1,982,174 effective search space used: 874138734 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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