Samia BLAST Result against nematostella

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 030723E4_D06_e332_08.seq
         (1553 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_30637| Best HMM Match : No HMM Matches (HMM E-Value=.)             144   2e-34
SB_49315| Best HMM Match : Calreticulin (HMM E-Value=0)                67   3e-11
SB_37182| Best HMM Match : DUF225 (HMM E-Value=1)                      30   5.7  

>SB_30637| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 1137

 Score =  144 bits (348), Expect = 2e-34
 Identities = 67/95 (70%), Positives = 74/95 (77%), Gaps = 1/95 (1%)
 Frame = +3

Query: 135  NCEVFFEEKFSDDSWESNWVYSEHPGKEFGKFKLTAGKFYNDAEADKGLQTSEDARFYAL 314
            N  V F EKF D SWE  WV S   G + GKFK TAGKFY DAEADKG+QTSEDA+FY +
Sbjct: 753  NPVVHFLEKFEDKSWEDRWVSSTSKGAQQGKFKWTAGKFYGDAEADKGIQTSEDAKFYGI 812

Query: 315  SRKF-KPFSNEGKPLVVQFSVKHEQDIDCGGGYLK 416
            S KF KPF+NEGK LV+QFSVKHEQ+IDCGGGY K
Sbjct: 813  SAKFEKPFTNEGKTLVIQFSVKHEQNIDCGGGYAK 847



 Score = 63.7 bits (148), Expect = 4e-10
 Identities = 50/162 (30%), Positives = 65/162 (40%)
 Frame = +3

Query: 507  KKVHVIFSYKGKNHLIKKDIRCKDDVYTHLYTLIVKPDNTYEVLSDNEKV*IW*IRSRLG 686
            K + + FS K + ++       KDD  THLYTLIV+PDNTYEV  DNEKV    +     
Sbjct: 825  KTLVIQFSVKHEQNIDCGGGYAKDDEMTHLYTLIVRPDNTYEVKIDNEKVESGELEKDWD 884

Query: 687  LPTXEEDQGSXSKXNLKIWDHXXXXXXXXXXXXXXDWDKPLSXISDP*XP*ALXIWMMRX 866
                +  +   +K   + WD               DWDKP   I DP        W    
Sbjct: 885  FLPPKTIKDPEAK-KPEDWD-DKKMIDDPEDKKPEDWDKP-QNIPDPDAK-KPEDWDDEI 940

Query: 867  XXXWNHPMXXTPDYXGXWAXXKLXPLLXRXXGXTPXXXXPEY 992
               W  PM   P+Y G W   ++     +     P    PEY
Sbjct: 941  DGEWEPPMIDNPEYKGEWKPKQIDNPNYKGEWVHPEIDNPEY 982



 Score = 44.8 bits (101), Expect = 2e-04
 Identities = 19/25 (76%), Positives = 20/25 (80%)
 Frame = +1

Query: 652 RFESGELEADWDFLPXKKIKDPXAK 726
           + ESGELE DWDFLP K IKDP AK
Sbjct: 873 KVESGELEKDWDFLPPKTIKDPEAK 897



 Score = 41.1 bits (92), Expect = 0.002
 Identities = 19/43 (44%), Positives = 19/43 (44%)
 Frame = +2

Query: 842  PEXLDDEXXXXXEPPYDXXPXLXGRLGPXQIXTPAXKGPWXHP 970
            PE  DDE     EPP    P   G   P QI  P  KG W HP
Sbjct: 933  PEDWDDEIDGEWEPPMIDNPEYKGEWKPKQIDNPNYKGEWVHP 975


>SB_49315| Best HMM Match : Calreticulin (HMM E-Value=0)
          Length = 1086

 Score = 67.3 bits (157), Expect = 3e-11
 Identities = 39/87 (44%), Positives = 50/87 (57%), Gaps = 10/87 (11%)
 Frame = +3

Query: 426 CRLDQKDMHGETPYEIMFGPDICGPGTKKVHVIFSYKG-KNHLIKKDIRCK--------- 575
           C L Q+    +TPY IMFGPD CG   +K+H IF +K  KN  I++    K         
Sbjct: 28  CHLFQESFGDKTPYTIMFGPDKCGED-RKLHFIFRHKNPKNGTIEEKHAKKPTGNYNSVF 86

Query: 576 DDVYTHLYTLIVKPDNTYEVLSDNEKV 656
           D   THL+TL+V+PDNT+EV  D E V
Sbjct: 87  DGKKTHLFTLVVRPDNTFEVFIDQESV 113


>SB_37182| Best HMM Match : DUF225 (HMM E-Value=1)
          Length = 1282

 Score = 29.9 bits (64), Expect = 5.7
 Identities = 12/32 (37%), Positives = 21/32 (65%), Gaps = 1/32 (3%)
 Frame = -2

Query: 400 PQSMSCSCFTLNCTTKGLP-SLLNGLNFLDRA 308
           P + SC CF + C+ + +P S+L  +++L RA
Sbjct: 150 PIACSCRCFPITCSCRCVPQSMLMSMSYLSRA 181


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 32,602,192
Number of Sequences: 59808
Number of extensions: 593907
Number of successful extensions: 1204
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 1093
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1200
length of database: 16,821,457
effective HSP length: 85
effective length of database: 11,737,777
effective search space used: 5070719664
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)