BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= 030723E4_D06_e332_08.seq (1553 letters) Database: mosquito 2352 sequences; 563,979 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AF457551-1|AAL68781.1| 406|Anopheles gambiae calreticulin protein. 331 3e-92 DQ137801-1|AAZ78362.1| 622|Anopheles gambiae male-specific doub... 28 0.63 AJ010193-1|CAA09032.1| 684|Anopheles gambiae prophenoloxidase p... 25 4.5 AJ535206-1|CAD59406.1| 1376|Anopheles gambiae SMC4 protein protein. 25 7.8 >AF457551-1|AAL68781.1| 406|Anopheles gambiae calreticulin protein. Length = 406 Score = 331 bits (814), Expect = 3e-92 Identities = 162/286 (56%), Positives = 186/286 (65%) Frame = +3 Query: 135 NCEVFFEEKFSDDSWESNWVYSEHPGKEFGKFKLTAGKFYNDAEADKGLQTSEDARFYAL 314 N +V+FEE F DDSW+ WV SEH G E+GKF TAGKFYNDAEADKGLQTS+DARFYAL Sbjct: 15 NAKVYFEEGFKDDSWQKTWVQSEHKGVEYGKFVHTAGKFYNDAEADKGLQTSQDARFYAL 74 Query: 315 SRKFKPFSNEGKPLVVQFSVKHEQDIDCGGGYLKVFDCRLDQKDMHGETPYEIMFGPDIC 494 S KF PFSN+ LV+QFSVKHEQ+IDCGGGYLKVFDC +DQKD+HGETPY +MFGPDIC Sbjct: 75 SNKFTPFSNKDDTLVIQFSVKHEQNIDCGGGYLKVFDCSVDQKDLHGETPYLVMFGPDIC 134 Query: 495 GPGTKKVHVIFSYKGKNHLIKKDIRCKDDVYTHLYTLIVKPDNTYEVLSDNEKV*IW*IR 674 GPGTKKVHVIFSYKGKNHLI KDIRCKDDV+TH YTL+V+ DNTYEVL DNEKV + Sbjct: 135 GPGTKKVHVIFSYKGKNHLINKDIRCKDDVFTHFYTLVVRADNTYEVLIDNEKVESGSLE 194 Query: 675 SRLGLPTXEEDQGSXSKXNLKIWDHXXXXXXXXXXXXXXDWDKPLSXISDP*XP*ALXIW 854 ++ + +K + WD DWDKP I DP W Sbjct: 195 DDWDFLPPKKIKDPEAK-KPEDWD-DRATIADPDDTKPEDWDKP-EHIPDPDAT-KPDDW 250 Query: 855 MMRXXXXWNHPMXXTPDYXGXWAXXKLXPLLXRXXGXTPXXXXPEY 992 W PM P+Y G W ++ + P PEY Sbjct: 251 DDEMDGEWEPPMIDNPEYKGEWKPKQIDNPAYKGVWVHPEIDNPEY 296 Score = 44.8 bits (101), Expect = 7e-06 Identities = 19/25 (76%), Positives = 20/25 (80%) Frame = +1 Query: 652 RFESGELEADWDFLPXKKIKDPXAK 726 + ESG LE DWDFLP KKIKDP AK Sbjct: 187 KVESGSLEDDWDFLPPKKIKDPEAK 211 Score = 43.6 bits (98), Expect = 2e-05 Identities = 26/80 (32%), Positives = 32/80 (40%) Frame = +2 Query: 731 PEDLGPQTHXSPDPEDNETRXLGQTLEXHFGPLMXVSPEXLDDEXXXXXEPPYDXXPXLX 910 PED + + DP+D + + H P+ DDE EPP P Sbjct: 213 PEDWDDRATIA-DPDDTKPEDWDKP--EHIPDPDATKPDDWDDEMDGEWEPPMIDNPEYK 269 Query: 911 GRLGPXQIXTPAXKGPWXHP 970 G P QI PA KG W HP Sbjct: 270 GEWKPKQIDNPAYKGVWVHP 289 >DQ137801-1|AAZ78362.1| 622|Anopheles gambiae male-specific doublesex protein protein. Length = 622 Score = 28.3 bits (60), Expect = 0.63 Identities = 13/27 (48%), Positives = 16/27 (59%) Frame = +2 Query: 731 PEDLGPQTHXSPDPEDNETRXLGQTLE 811 PEDL TH SP+ EDN ++ LE Sbjct: 405 PEDLHTTTHKSPEREDNPSQPYEAYLE 431 >AJ010193-1|CAA09032.1| 684|Anopheles gambiae prophenoloxidase protein. Length = 684 Score = 25.4 bits (53), Expect = 4.5 Identities = 9/30 (30%), Positives = 18/30 (60%) Frame = +3 Query: 516 HVIFSYKGKNHLIKKDIRCKDDVYTHLYTL 605 H+++ +G N +++KD R + Y H T+ Sbjct: 213 HLVYPARGPNRIVRKDRRGELFYYMHQQTM 242 >AJ535206-1|CAD59406.1| 1376|Anopheles gambiae SMC4 protein protein. Length = 1376 Score = 24.6 bits (51), Expect = 7.8 Identities = 7/22 (31%), Positives = 16/22 (72%) Frame = -1 Query: 260 VIVELSSSQFELAEFFSGVFTI 195 +I+ L S+ FEL+++ G++ + Sbjct: 1243 IIISLRSNMFELSDYLVGIYKV 1264 Database: mosquito Posted date: Oct 23, 2007 1:18 PM Number of letters in database: 563,979 Number of sequences in database: 2352 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,099,364 Number of Sequences: 2352 Number of extensions: 19461 Number of successful extensions: 30 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 26 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 30 length of database: 563,979 effective HSP length: 67 effective length of database: 406,395 effective search space used: 182877750 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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